If you have the patient’s absolute lymphocyte count (ALC), call 513-636-4685 or use the "Customized Volumes by ALC" sheet to obtain the precise amount of blood required for this assay. If you do not have an ALC available, 5-10mL blood in Sodium Heparin (Dark Green) is the general requirement and should be sufficient for patients who are not lymphopenic.
Minimum Collection Volume
Minimum volume dependent on ALC
Unacceptable Conditions
Frozen, clotted, hemolyzed or centrifuged specimens. Specimens collected in anticoagulants other than Sodium Heparin. Specimens received greater than 24 hours after collection.
Storage/Transport Temperature
CRITICAL AMBIENT
Specimen Preparation
Room Temp. Specimen must be received by our laboratory within 24 hours of collection.
Peripheral blood lymphocytes that have previously been exposed in vivo to antigens become sensitized. If they are subsequently exposed in vitro to those same antigens, they will be triggered to initiate an immune response that culminates in proliferation. This assay uses the antigens Candida Albicans and tetanus toxoid to stimulate a purified mononuclear cell preparation and test for the ability of the cells to respond by proliferating. An excess of radio-labeled thymidine is made available to the cells during the later part of the culture period. Cells undergoing DNA synthesis will incorporate the thymidine, which can then be measured using a microplate scintillation counter.
CPT Codes
86353x2
Collection
Notes
This test is New York DOH approved.
Collect
If you have the patient’s absolute lymphocyte count (ALC), call 513-636-4685 or use the "Customized Volumes by ALC" sheet to obtain the precise amount of blood required for this assay. If you do not have an ALC available, 5-10mL blood in Sodium Heparin (Dark Green) is the general requirement and should be sufficient for patients who are not lymphopenic.
Minimum Collection Volume
Minimum volume dependent on ALC
Unacceptable Conditions
Frozen, clotted, hemolyzed or centrifuged specimens. Specimens collected in anticoagulants other than Sodium Heparin. Specimens received greater than 24 hours after collection.
Storage/Transport Temperature
CRITICAL AMBIENT
Specimen Preparation
Room Temp. Specimen must be received by our laboratory within 24 hours of collection.
Peripheral blood lymphocytes that have previously been exposed in vivo to antigens become sensitized. If they are subsequently exposed in vitro to those same antigens, they will be triggered to initiate an immune response that culminates in proliferation. This assay uses the antigens Candida Albicans and tetanus toxoid to stimulate a purified mononuclear cell preparation and test for the ability of the cells to respond by proliferating. An excess of radio-labeled thymidine is made available to the cells during the later part of the culture period. Cells undergoing DNA synthesis will incorporate the thymidine, which can then be measured using a microplate scintillation counter.
