Collect

One 5.0 mL EDTA (Lavender) tube.
5.0 mL whole blood or 3.0 mL minimum.

Unacceptable Conditions

The specimen will be considered unacceptable if it is refrigerated, clotted, hemalyzed, centrifuged, received in the lab over 48 hours from collection.

Storage/Transport Temperature

Ambient.

Stability (from collection to initiation)

Will accept up to within 48 hours if there is a CBC result performed on a specimen collected the same time as the specimen.

Performed

Flow Cytometry.
Monday-Friday (Specimens must be received by 1100AM on Friday)
 

Remarks

Provide CBC results performed on a specimen collected the same time as the specimen for the lymph assay.

Notes

Specimen should be received in the lab within 24 hours of collection.  If specimen is greater than 24-48 hours, a CBC result performed on a specimen collected at the same time MUST accompany specimen.

Performed

Flow Cytometry.
Monday-Friday (Specimens must be received by 1100AM on Friday)
 

Reported

Results in 24 hours.

Synonyms

  • CD4/CD8

Reference Interval

 0-3 mo3-6 mo6-12 mo1-2 yr2-5 yr6-11 yr12-18 yr>19 year
 %/cmm%/cmm%/cmm%/cmm%/cmm%/cmm%/cmm%/cmm
CD353-842500-550051-772500-560049-761900-590053-752100-620056-751400-370060-761200-260056-841000-220061.0-88.6427-3455
CD435-641600-400035-561800-400031-561400-430032-511300-340028-47700-220031-47650-150031-52530-130033.3-65.5233-2555
CD812-28560-170012-23590-160012-24500-170014-30620-200016-30490-130018-35370-110018-35330-9209.1-38.364-1494
4/8 RATIO>1.0

 

CPT Codes

86359, 86360

Test Build Information

OrderCodeOrderNameResultCodeResultNameResultUnitsRESULT LOINCCPT
T4T8Lymphocyte 4/8 RatioCD3PQCD3%20599-786359, 86360
T4T8Lymphocyte 4/8 RatioCD3NQCD3/cmm20598-9 
T4T8Lymphocyte 4/8 RatioCD4PQCD4%8123-2 
T4T8Lymphocyte 4/8 RatioCD4NQCD4/cmm32515-9 
T4T8Lymphocyte 4/8 RatioCD8PQCD8%8101-8 
T4T8Lymphocyte 4/8 RatioCD8NQCD8/cmm14135-8 
T4T8Lymphocyte 4/8 RatioR48CD4/CD8 RATIO 54218-3 
T4T8Lymphocyte 4/8 RatioTERP1INTERPRETATION 50595-8 

REFLEX if CD3 <50%
 

Example Report

Additional Information

For additional information or questions, contact RPSInterfaceSupport@unmc.edu
 
Collection

Collect

One 5.0 mL EDTA (Lavender) tube.
5.0 mL whole blood or 3.0 mL minimum.

Unacceptable Conditions

The specimen will be considered unacceptable if it is refrigerated, clotted, hemalyzed, centrifuged, received in the lab over 48 hours from collection.

Storage/Transport Temperature

Ambient.

Stability (from collection to initiation)

Will accept up to within 48 hours if there is a CBC result performed on a specimen collected the same time as the specimen.

Performed

Flow Cytometry.
Monday-Friday (Specimens must be received by 1100AM on Friday)
 

Remarks

Provide CBC results performed on a specimen collected the same time as the specimen for the lymph assay.

Notes

Specimen should be received in the lab within 24 hours of collection.  If specimen is greater than 24-48 hours, a CBC result performed on a specimen collected at the same time MUST accompany specimen.

Ordering

Performed

Flow Cytometry.
Monday-Friday (Specimens must be received by 1100AM on Friday)
 

Reported

Results in 24 hours.

Synonyms

  • CD4/CD8
Result Interpretation

Reference Interval

 0-3 mo3-6 mo6-12 mo1-2 yr2-5 yr6-11 yr12-18 yr>19 year
 %/cmm%/cmm%/cmm%/cmm%/cmm%/cmm%/cmm%/cmm
CD353-842500-550051-772500-560049-761900-590053-752100-620056-751400-370060-761200-260056-841000-220061.0-88.6427-3455
CD435-641600-400035-561800-400031-561400-430032-511300-340028-47700-220031-47650-150031-52530-130033.3-65.5233-2555
CD812-28560-170012-23590-160012-24500-170014-30620-200016-30490-130018-35370-110018-35330-9209.1-38.364-1494
4/8 RATIO>1.0

 

Administrative

CPT Codes

86359, 86360
RPS Interface Information

Test Build Information

OrderCodeOrderNameResultCodeResultNameResultUnitsRESULT LOINCCPT
T4T8Lymphocyte 4/8 RatioCD3PQCD3%20599-786359, 86360
T4T8Lymphocyte 4/8 RatioCD3NQCD3/cmm20598-9 
T4T8Lymphocyte 4/8 RatioCD4PQCD4%8123-2 
T4T8Lymphocyte 4/8 RatioCD4NQCD4/cmm32515-9 
T4T8Lymphocyte 4/8 RatioCD8PQCD8%8101-8 
T4T8Lymphocyte 4/8 RatioCD8NQCD8/cmm14135-8 
T4T8Lymphocyte 4/8 RatioR48CD4/CD8 RATIO 54218-3 
T4T8Lymphocyte 4/8 RatioTERP1INTERPRETATION 50595-8 

REFLEX if CD3 <50%
 

Example Report

Additional Information

For additional information or questions, contact RPSInterfaceSupport@unmc.edu