UCHealth North Laboratory Test Directory

Epic ID

LAB3705

Performing Lab

ARUP Laboratories

Collect

Serum separator tube (SST), red top tube

Specimen Preparation

Separate from cells ASAP or within 2 hours of collection. Transfer 3 to 4 mL to ARUP standard transport tube. (Min: 1.5 mL)

Unacceptable Conditions

Hemolyzed, hyperlipemic, icteric, heat-treated, or contaminated specimens.

Storage/Transport Temperature

Refrigerated

Stability (from collection to initiation)

Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 month

Performed

Sun-Sat

Notes

Antibodies: Mi-2, PL-7, PL12, P155/140, EJ, Ku, OJ, PM/Scl, SRP, Smith/RNP, Ro52, Ro60, Jo-1, U3 Fib, SAE1, NXP2, MDA5, TIF1-gamma, ANA, Ha, Ks, Zo

If HMGCR antibody, IgG is positive, additional testing to follow.

Referral Lab

Laboratories.

Performed

Sun-Sat

Methodology

Semi-Quantitative Enzyme-Linked Immunosorbent Assay (ELISA) / Qualitative Immunoprecipitation / Semi-Quantitative Multiplex Bead Assay / Qualitative Immunoblot / Semi-Quantitative Indirect Fluorescent Antibody (IFA) / Qualitative Particle-Based Multianalyte Technology (PMAT)

Reported

7-18 days

Additional Technical Information

Additional Technical Information

Ordering Recommendations

May be useful for differential evaluation of polymyositis, dermatomyositis, necrotizing autoimmune myopathy, or overlap syndromes associated with connective tissue disease.

Epic ID

LAB3705

Reference Interval

Components	Reference Interval
HMGCR Antibody Screen	Negative
Zo (phenylalanyl-tRNA synthetase) Ab	Negative
Ks (asparaginyl-tRNA synthetase) Ab	Negative
Ha (tyrosyl-tRNA synthetase) Ab	Negative
TIF-1 gamma (155 kDa) Ab	Negative
NXP2 (Nuclear matrix protein-2) Ab	Negative
MDA5 (CADM-140) Ab	Negative
SAE1 (SUMO activating enzyme) Ab	Negative
Antinuclear Antibody (ANA), HEp-2, IgG	Less than 1:80
Fibrillarin (U3 RNP) Ab, IgG	Negative
SSA-60 (Ro60) (ENA) Antibody, IgG	40 AU/mL or less
OJ (isoleucyl-tRNA synthetase) Antibody	Negative
SRP (Signal Recognition Particle) Ab	Negative
Ku Antibody	Negative
EJ (glycyl-tRNA synthetase) Antibody	Negative
P155/140 Antibody	Negative
PL-12 (alanyl-tRNA synthetase) Antibody	Negative
PL-7 (threonyl-tRNA synthetase) Antibody	Negative
Mi-2 (nuclear helicase protein) Antibody	Negative
PM/Scl 100 Antibody, IgG	Negative
Jo-1 (Histidyl-tRNA Synthetase) Ab, IgG	40 AU/mL or less
SSA-52 (Ro52) (ENA) Antibody, IgG	40 AU/mL or less
Smith/RNP (ENA) Ab, IgG	19 Units or less

Interpretive Data

Refer to report.

Component	Interpretation
SSA-52 (Ro52) (ENA) Antibody, IgG	29 AU/mL or less: Negative 30-40 AU/mL: Equivocal 41 AU/mL or greater: Positive
SSA-60 (Ro60) (ENA) Antibody, IgG	29 AU/mL or less: Negative 30-40 AU/mL: Equivocal 41 AU/mL or greater: Positive
Smith/RNP (ENA) Antibody, IgG	19 Units or less: Negative 20-39 Units: Weak Positive 40-80 Units: Moderate Positive 81 Units or greater: Strong Positive
Jo-1 Antibody, IgG	29 AU/mL or less: Negative 30-40 AU/mL: Equivocal 41 AU/mL or greater: Positive

CPT Codes

83516 x13; 84182 x3; 86039; 86235 x6; if reflexed, add 83516

Notes

Collection

Epic ID

LAB3705

Performing Lab

ARUP Laboratories

Collect

Serum separator tube (SST), red top tube

Specimen Preparation

Separate from cells ASAP or within 2 hours of collection. Transfer 3 to 4 mL to ARUP standard transport tube. (Min: 1.5 mL)

Unacceptable Conditions

Hemolyzed, hyperlipemic, icteric, heat-treated, or contaminated specimens.

Storage/Transport Temperature

Refrigerated

Stability (from collection to initiation)

Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 month

Performed

Sun-Sat

Notes

Referral Lab

Laboratories.

Ordering

Performed

Sun-Sat

Methodology

Reported

7-18 days

Additional Technical Information

Additional Technical Information

Ordering Recommendations

May be useful for differential evaluation of polymyositis, dermatomyositis, necrotizing autoimmune myopathy, or overlap syndromes associated with connective tissue disease.

Epic ID

LAB3705

Result Interpretation

Reference Interval

Components	Reference Interval
HMGCR Antibody Screen	Negative
Zo (phenylalanyl-tRNA synthetase) Ab	Negative
Ks (asparaginyl-tRNA synthetase) Ab	Negative
Ha (tyrosyl-tRNA synthetase) Ab	Negative
TIF-1 gamma (155 kDa) Ab	Negative
NXP2 (Nuclear matrix protein-2) Ab	Negative
MDA5 (CADM-140) Ab	Negative
SAE1 (SUMO activating enzyme) Ab	Negative
Antinuclear Antibody (ANA), HEp-2, IgG	Less than 1:80
Fibrillarin (U3 RNP) Ab, IgG	Negative
SSA-60 (Ro60) (ENA) Antibody, IgG	40 AU/mL or less
OJ (isoleucyl-tRNA synthetase) Antibody	Negative
SRP (Signal Recognition Particle) Ab	Negative
Ku Antibody	Negative
EJ (glycyl-tRNA synthetase) Antibody	Negative
P155/140 Antibody	Negative
PL-12 (alanyl-tRNA synthetase) Antibody	Negative
PL-7 (threonyl-tRNA synthetase) Antibody	Negative
Mi-2 (nuclear helicase protein) Antibody	Negative
PM/Scl 100 Antibody, IgG	Negative
Jo-1 (Histidyl-tRNA Synthetase) Ab, IgG	40 AU/mL or less
SSA-52 (Ro52) (ENA) Antibody, IgG	40 AU/mL or less
Smith/RNP (ENA) Ab, IgG	19 Units or less

Interpretive Data

Refer to report.

Component	Interpretation
SSA-52 (Ro52) (ENA) Antibody, IgG	29 AU/mL or less: Negative 30-40 AU/mL: Equivocal 41 AU/mL or greater: Positive
SSA-60 (Ro60) (ENA) Antibody, IgG	29 AU/mL or less: Negative 30-40 AU/mL: Equivocal 41 AU/mL or greater: Positive
Smith/RNP (ENA) Antibody, IgG	19 Units or less: Negative 20-39 Units: Weak Positive 40-80 Units: Moderate Positive 81 Units or greater: Strong Positive
Jo-1 Antibody, IgG	29 AU/mL or less: Negative 30-40 AU/mL: Equivocal 41 AU/mL or greater: Positive

Administrative

CPT Codes

83516 x13; 84182 x3; 86039; 86235 x6; if reflexed, add 83516

Notes

ARUP Consult®

Disease Topics

Epic ID

Performing Lab

Collect

Specimen Preparation

Unacceptable Conditions

Storage/Transport Temperature

Stability (from collection to initiation)

Performed

Notes

Referral Lab

Performed

Methodology

Reported

Additional Technical Information

Ordering Recommendations

Epic ID

Reference Interval

Interpretive Data

CPT Codes

Notes

Epic ID

Performing Lab

Collect

Specimen Preparation

Unacceptable Conditions

Storage/Transport Temperature

Stability (from collection to initiation)

Performed

Notes

Referral Lab

Performed

Methodology

Reported

Additional Technical Information

Ordering Recommendations

Epic ID

Reference Interval

Interpretive Data

CPT Codes

Notes

ARUP Consult®

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