Epic ID

LAB 3646

Performing Lab

National Jewish Medical and Research Center

Collect

Requires special handling: 20 mL green top [heparinized - Sodium or Lithium] - whole blood. (Dark Green)

SCHEDULE W/LAB PRIOR TO COLLECTION for Bactericidal and Chemotaxis testing.

Call 720-848-7029.

Specimen Preparation

Green tops with gel (PST) acceptable if they are NOT centrifuged (per NJH Immunology Lab).

Unacceptable Conditions

Refrigerated/frozen samples. Samples >24hours old.

Storage/Transport Temperature

Internal: Deliver to lab immediatley. Mon-Thurs only.
Offsite: 20 mL whole blood in plain green top tube Deliver to lab same day. Mon-Thurs only.

Stability (from collection to initiation)

Ambient specimen must be received by Reference lab within 24 hours of collection [testing is performed on live neutrophils.]

Performed

Upon receipt

Remarks

Various neutrophil function assays available:
Bactericidal - Needs to be scheduled PRIOR to collection.

Chemotaxis - Needs to be scheduled PRIOR to collection.

Please contact the Clinical Lab - Senoduts @ 720-848-7043
M-Th to schedule testing.

Nitroblue Tetrazolium [NBT] dye reduction

Dihydrorhodamine [DHR] (oxidative metabolism).

Performed

Upon receipt

Methodology

Various methods of stimulating neutrophils at various stages of their phogacytic process are used to determine the host neutrophil's phagocytic ability.

Reported

Varies

Synonyms

  • CHEMOTAXIS
  • NBT - NitroblueTetrazolium
  • DHR - Dihydrorhodamine
  • NEUTFUNC
  • NFT

Epic ID

LAB 3646

CPT Codes

Varies depending on function type:
86344 Bactericidal
86155 x2 Chemotaxis
82657 x2 DHR
86384 NBT

Collection

Epic ID

LAB 3646

Performing Lab

National Jewish Medical and Research Center

Collect

Requires special handling: 20 mL green top [heparinized - Sodium or Lithium] - whole blood. (Dark Green)

SCHEDULE W/LAB PRIOR TO COLLECTION for Bactericidal and Chemotaxis testing.

Call 720-848-7029.

Specimen Preparation

Green tops with gel (PST) acceptable if they are NOT centrifuged (per NJH Immunology Lab).

Unacceptable Conditions

Refrigerated/frozen samples. Samples >24hours old.

Storage/Transport Temperature

Internal: Deliver to lab immediatley. Mon-Thurs only.
Offsite: 20 mL whole blood in plain green top tube Deliver to lab same day. Mon-Thurs only.

Stability (from collection to initiation)

Ambient specimen must be received by Reference lab within 24 hours of collection [testing is performed on live neutrophils.]

Performed

Upon receipt

Remarks

Various neutrophil function assays available:
Bactericidal - Needs to be scheduled PRIOR to collection.

Chemotaxis - Needs to be scheduled PRIOR to collection.

Please contact the Clinical Lab - Senoduts @ 720-848-7043
M-Th to schedule testing.

Nitroblue Tetrazolium [NBT] dye reduction

Dihydrorhodamine [DHR] (oxidative metabolism).

Ordering

Performed

Upon receipt

Methodology

Various methods of stimulating neutrophils at various stages of their phogacytic process are used to determine the host neutrophil's phagocytic ability.

Reported

Varies

Synonyms

  • CHEMOTAXIS
  • NBT - NitroblueTetrazolium
  • DHR - Dihydrorhodamine
  • NEUTFUNC
  • NFT

Epic ID

LAB 3646
Result Interpretation
Administrative

CPT Codes

Varies depending on function type:
86344 Bactericidal
86155 x2 Chemotaxis
82657 x2 DHR
86384 NBT