Anti-MICA antibodies detected by specialized microspheres coupled with purified MICA antigens against single MICA specificities. The specificity of the antibody is reported. Test is performed by flow cytometry.
Performing Laboratory / Facility
UCLA Immunogenetics Center
Performing Section
Immunogenetics
Availability
Monday through Friday, 0800-1700
Turnaround Time
21 days
Methodology
Patient serum is tested against a population of specialized microparticles coupled with a single purified MICA antigen. Reactions are detected by flow cytometry.
Use
Organ transplant candidates. The test identifies patients at risk for antibody-mediated damage to grafted organs or tissue.
Patient Preparation
Draw specimen before hemodialysis of patient.
Specimen Type
Blood
Container
Red
Collection Instructions
Deliver to Specimen Processing.
Volume
10 mL
Minimum Volume
Adult: 3 mL (1.5 mL serum) Pediatric: 1 mL (0.5 mL serum)
Stability
Keep blood at room temperature and transport samples as soon as possible after collection.
Shipping and Handling Instructions
Store at room temperature.
Causes for Rejection
Specimen grossly hemolyzed Improper labeling of tubes or requisition
Anti-MICA antibodies detected by specialized microspheres coupled with purified MICA antigens against single MICA specificities. The specificity of the antibody is reported. Test is performed by flow cytometry.
Performing Laboratory / Facility
UCLA Immunogenetics Center
Performing Section
Immunogenetics
Availability
Monday through Friday, 0800-1700
Turnaround Time
21 days
Methodology
Patient serum is tested against a population of specialized microparticles coupled with a single purified MICA antigen. Reactions are detected by flow cytometry.
Use
Organ transplant candidates. The test identifies patients at risk for antibody-mediated damage to grafted organs or tissue.
Patient Preparation
Draw specimen before hemodialysis of patient.
Specimen Collection and Handling
Specimen Type
Blood
Container
Red
Collection Instructions
Deliver to Specimen Processing.
Volume
10 mL
Minimum Volume
Adult: 3 mL (1.5 mL serum) Pediatric: 1 mL (0.5 mL serum)
Stability
Keep blood at room temperature and transport samples as soon as possible after collection.
Shipping and Handling Instructions
Store at room temperature.
Causes for Rejection
Specimen grossly hemolyzed Improper labeling of tubes or requisition
