BMT Post-transplant follow-up: 81267 with cell selection CD3 81268 with cell selection CD33 81268 if it is the first Post transplant add 81265
Synonyms
BMT Donor DNA Isolation
BMT Recipient pre-transplant DNA Isolation
BMT Recipient Pre-TX
Bone Marrow Chimerism
Bone Marrow Donor
Bone Marrow Engraftment Monitoring
STR
Test Includes
Recipient Pre-transplant, BMT donor, and post-transplant follow up specimen genotyping
Performing Laboratory / Facility
UCLA Medical Center Clinical Laboratory (CHS)
Performing Section
Molecular Pathology
Availability
Monday through Friday, 0700-1700
Turnaround Time
14 days after receipt all of the pretransplant recipient, donor and post-transplant specimens in the laboratory
Methodology
Polymerase Chain Reaction (PCR) analysis of polymorphic short tandem repeats to distinguish recipient from donor DNA
Use
A useful tool for determining whether or not a bone marrow transplant has engrafted is a PCR method comparing the DNA pattern from the recipient to that of the donor, and establishing a polymorphism to differentiate between the recipient and the donor DNA. By sampling the DNA from the recipient 10-30 days after transplant and comparing it to the donor, we are able to tell the degree of engraftment or nonengraftment.
See Also
87091 DNA Identity
Applies To
Short Tandem Repeat (STR) STR
Limitations
Very rarely it may be difficult or impossible to detect a polymorphic difference between the donor and recipient DNA, especially in sibling transplants. Use of the PCR method will sometimes reveal trace amounts of recipient DNA post-transplant that are of uncertain clinical significance, perhaps representing bone marrow stromal cells.
Specimen Type
Whole blood, Bone marrow, Buccal Mucosal Cells, Extracted DNA (must be extracted by CLIA-certified laboratories or laboratories meeting equivalent requirements)
Donor DNA present; recipient DNA presence excluded to a level of 5%. By observing donor and/or recipient-specific DNA patterns in the post-transplant specimen, engraftment, nonengraftment, or partial engraftment (mixed chimerisms) can be determined. In the case of a mixture, the relative proportions of donor and recipient DNA can be estimated semiquantitatively by comparing intensity of the donor and recipient DNA peaks.
Test Information
CPT Codes
BMT Post-transplant follow-up: 81267 with cell selection CD3 81268 with cell selection CD33 81268 if it is the first Post transplant add 81265
Synonyms
BMT Donor DNA Isolation
BMT Recipient pre-transplant DNA Isolation
BMT Recipient Pre-TX
Bone Marrow Chimerism
Bone Marrow Donor
Bone Marrow Engraftment Monitoring
STR
Test Includes
Recipient Pre-transplant, BMT donor, and post-transplant follow up specimen genotyping
Performing Laboratory / Facility
UCLA Medical Center Clinical Laboratory (CHS)
Performing Section
Molecular Pathology
Availability
Monday through Friday, 0700-1700
Turnaround Time
14 days after receipt all of the pretransplant recipient, donor and post-transplant specimens in the laboratory
Methodology
Polymerase Chain Reaction (PCR) analysis of polymorphic short tandem repeats to distinguish recipient from donor DNA
Use
A useful tool for determining whether or not a bone marrow transplant has engrafted is a PCR method comparing the DNA pattern from the recipient to that of the donor, and establishing a polymorphism to differentiate between the recipient and the donor DNA. By sampling the DNA from the recipient 10-30 days after transplant and comparing it to the donor, we are able to tell the degree of engraftment or nonengraftment.
See Also
87091 DNA Identity
Applies To
Short Tandem Repeat (STR) STR
Limitations
Very rarely it may be difficult or impossible to detect a polymorphic difference between the donor and recipient DNA, especially in sibling transplants. Use of the PCR method will sometimes reveal trace amounts of recipient DNA post-transplant that are of uncertain clinical significance, perhaps representing bone marrow stromal cells.
Specimen Collection and Handling
Specimen Type
Whole blood, Bone marrow, Buccal Mucosal Cells, Extracted DNA (must be extracted by CLIA-certified laboratories or laboratories meeting equivalent requirements)
Donor DNA present; recipient DNA presence excluded to a level of 5%. By observing donor and/or recipient-specific DNA patterns in the post-transplant specimen, engraftment, nonengraftment, or partial engraftment (mixed chimerisms) can be determined. In the case of a mixture, the relative proportions of donor and recipient DNA can be estimated semiquantitatively by comparing intensity of the donor and recipient DNA peaks.
