Compare specific DNA patterns (polymorphism) between specimens
Performing Laboratory / Facility
UCLA Medical Center Clinical Laboratory (CHS)
Performing Section
Molecular Pathology
Availability
Monday through Friday, 0700-1700
Turnaround Time
14 days after receipt of all of the specimens
Methodology
The GenePrint® 24 System from Promega uses a one-tube multiplex PCR reaction to simultaneously interrogate 24 DNA loci from a variety of human-derived biological sources.
Use
Establish and compare specific DNA patterns (polymorphisms) between individuals, tissues, or specimens for purposes of identification or lineage
Patient Preparation
Patient should receive no transfusions 90 days prior to testing.
Comment
2 different specimens from appropriate specimen types are required for this test
Additional Information
The genetic material of humans is highly polymorphic, and an individual's genotype represents a unique pattern which determines that person's identity and heredity. The only exception to this rule is identical twins, since they are derived from a single fertilized egg and hence have the same DNA profile. As a general rule, DNA is constant in all tissues of the body (even prenatal samples such as amniotic cells and chorionic villi specimens). DNA isolated from any specimen from an individual will be identical, which can prove to be very valuable in forensic evidence. DNA typing provides a valuable tool for establishing family relationships. PCR tests using a panel of DNA primers specific for several polymorphic DNA regions can produce a composite profile which is unique to an individual and can be traced through families to establish relationships. The laboratory report, in the form of a confidential letter, explains in detail all the polymorphic DNA markers used, the results (match or mismatch) with each, and the cumulative probability of paternity or nonpaternity, or identity or nonidentity of specimens.
Fresh frozen tissue can be stored in a -70°C freezer until shipped.
Shipping and Handling Instructions
Transport specimen( other then frozen tissue) at room temperature Fresh frozen tissue transport on dry ice
Causes for Rejection
If the tissue specimen thaws out during transport to the laboratory or before shipping, DNA may not be obtained from the specimen If <0.2 grams of tissue is sent to the laboratory, it may not yield enough DNA for analysis Blood samples frozen and thawed (will yield low quality DNA) Broken slides or tubes
Test Information
CPT Codes
81265
Synonyms
DNA Genetic Identity
Specimen Identity
DNA Fingerprinting
Test Includes
Compare specific DNA patterns (polymorphism) between specimens
Performing Laboratory / Facility
UCLA Medical Center Clinical Laboratory (CHS)
Performing Section
Molecular Pathology
Availability
Monday through Friday, 0700-1700
Turnaround Time
14 days after receipt of all of the specimens
Methodology
The GenePrint® 24 System from Promega uses a one-tube multiplex PCR reaction to simultaneously interrogate 24 DNA loci from a variety of human-derived biological sources.
Use
Establish and compare specific DNA patterns (polymorphisms) between individuals, tissues, or specimens for purposes of identification or lineage
Patient Preparation
Patient should receive no transfusions 90 days prior to testing.
Comment
2 different specimens from appropriate specimen types are required for this test
Additional Information
The genetic material of humans is highly polymorphic, and an individual's genotype represents a unique pattern which determines that person's identity and heredity. The only exception to this rule is identical twins, since they are derived from a single fertilized egg and hence have the same DNA profile. As a general rule, DNA is constant in all tissues of the body (even prenatal samples such as amniotic cells and chorionic villi specimens). DNA isolated from any specimen from an individual will be identical, which can prove to be very valuable in forensic evidence. DNA typing provides a valuable tool for establishing family relationships. PCR tests using a panel of DNA primers specific for several polymorphic DNA regions can produce a composite profile which is unique to an individual and can be traced through families to establish relationships. The laboratory report, in the form of a confidential letter, explains in detail all the polymorphic DNA markers used, the results (match or mismatch) with each, and the cumulative probability of paternity or nonpaternity, or identity or nonidentity of specimens.
Fresh frozen tissue can be stored in a -70°C freezer until shipped.
Shipping and Handling Instructions
Transport specimen( other then frozen tissue) at room temperature Fresh frozen tissue transport on dry ice
Causes for Rejection
If the tissue specimen thaws out during transport to the laboratory or before shipping, DNA may not be obtained from the specimen If <0.2 grams of tissue is sent to the laboratory, it may not yield enough DNA for analysis Blood samples frozen and thawed (will yield low quality DNA) Broken slides or tubes
