Smears will be performed on respiratory samples. Separate charges will be billed for AFB smear and AFB culture. Additional charges will be billed for decontamination of samples from nonsterile sites.
Mycobacterium tuberculosis complex DNA is automatically performed on respiratory specimens with positive smears, and billed separately.
Synonyms
TB culture
AFB culture
tuberculosis
atypical mycobacteria
MAC
mycobacteriaum avium intercellulare complex
MAI
Sample Type
Sputum, BAL, bronchial wash
Collect
Urine cup or sterilecontainer
Amount to Collect
Sputum: 5 mL
BAL or Bronchial wash: 10 mL
Preferred Volume
Sputum: 5 mL
?BAL or Bronchial wash: 10 mL
Minimum Volume
Sputum: 1 mL
?BAL or Bronchial wash: 2 mL
Remarks
Sputum: Collect 3 separate sputum samples in 8-12 hour intervals, including at least one early morning sample.
Sputum samples collected < 8 hours apart are pooled & tested as a single specimen.
Stability (from collection to initiation)
Refrigerated 3 days.
Test Code
P285R
Test Group
Mycobacteria
Performing Lab
Microbiology
Specimen Preparation
Sputum: Before accessioning, check if a sample was submitted earlier. If earlier sample was collected < 8 hours apart from the sample just received, call China Basin to check if earlier sample has been processed.
1. If earlier sample has not been processed, TND as duplicate and enter code AFPOOL. Inform submitting location that the sample is being pooled with the prior sample, and to collect an additional sputum. Affix a note to the sample stating “Pool sample for AFB with (accession # of earlier sample)".
2. If earlier sample has been processed, TND as duplicate and freetext “Collect sputa for AFB culture 8 hours apart".
Bronchial lavage/wash: Aliquot the maximum amount of specimen available (>10 mL) into a blue top centrifuge tube.
Bronchial brush:
1. Use sterile forceps to transfer brush to a 50 ml blue-topped centrifuge tube.
2. Add NPC 67 Neutralizing buffer to make a total volume of 2 ml. 3. Vortex thoroughly to expel specimen from the brush and then remove brush from tube.
Preferred Volume
Sputum: 5 mL
?BAL or Bronchial wash: 10 mL
Minimum Volume
Sputum: 1 mL
?BAL or Bronchial wash: 2 mL
Stability (from collection to initiation)
Refrigerated 3 days.
Reference Interval
No AFB isolated
Critical Values
Inpatient results only. After hours outpatient results will be phoned the following morning.
First positive AFB smear, first positive AFB culture if smear negative or no smear, or first isolate of M. tuberculosis; Repeat call only for positive sample from different site or > 2 months since initial call.
CPT Codes
87116, 87206
Available Stat
No
Test Code
P285R
Test Group
Mycobacteria
Performing Lab
Microbiology
Performed
Daily, day shift
Methodology
Culture
Remarks
Sputum: Collect 3 separate sputum samples in 8-12 hour intervals, including at least one early morning sample.
Sputum samples collected < 8 hours apart are pooled & tested as a single specimen.
Collect
Urine cup or sterilecontainer
Amount to Collect
Sputum: 5 mL
BAL or Bronchial wash: 10 mL
Sample Type
Sputum, BAL, bronchial wash
Preferred Volume
Sputum: 5 mL
?BAL or Bronchial wash: 10 mL
Minimum Volume
Sputum: 1 mL
?BAL or Bronchial wash: 2 mL
Specimen Preparation
Sputum: Before accessioning, check if a sample was submitted earlier. If earlier sample was collected < 8 hours apart from the sample just received, call China Basin to check if earlier sample has been processed.
1. If earlier sample has not been processed, TND as duplicate and enter code AFPOOL. Inform submitting location that the sample is being pooled with the prior sample, and to collect an additional sputum. Affix a note to the sample stating “Pool sample for AFB with (accession # of earlier sample)".
2. If earlier sample has been processed, TND as duplicate and freetext “Collect sputa for AFB culture 8 hours apart".
Bronchial lavage/wash: Aliquot the maximum amount of specimen available (>10 mL) into a blue top centrifuge tube.
Bronchial brush:
1. Use sterile forceps to transfer brush to a 50 ml blue-topped centrifuge tube.
2. Add NPC 67 Neutralizing buffer to make a total volume of 2 ml. 3. Vortex thoroughly to expel specimen from the brush and then remove brush from tube.
Reference Interval
No AFB isolated
Critical Values
Inpatient results only. After hours outpatient results will be phoned the following morning.
First positive AFB smear, first positive AFB culture if smear negative or no smear, or first isolate of M. tuberculosis; Repeat call only for positive sample from different site or > 2 months since initial call.
Synonyms
TB culture
AFB culture
tuberculosis
atypical mycobacteria
MAC
mycobacteriaum avium intercellulare complex
MAI
Stability (from collection to initiation)
Refrigerated 3 days.
Reported
Up to 7 weeks
Reflex Testing
Smears will be performed on respiratory samples. Separate charges will be billed for AFB smear and AFB culture. Additional charges will be billed for decontamination of samples from nonsterile sites.
Mycobacterium tuberculosis complex DNA is automatically performed on respiratory specimens with positive smears, and billed separately.
CPT Codes
87116, 87206
Ordering
Available Stat
No
Performing Lab
Microbiology
Performed
Daily, day shift
Methodology
Culture
Reported
Up to 7 weeks
Reflex Testing
Smears will be performed on respiratory samples. Separate charges will be billed for AFB smear and AFB culture. Additional charges will be billed for decontamination of samples from nonsterile sites.
Mycobacterium tuberculosis complex DNA is automatically performed on respiratory specimens with positive smears, and billed separately.
Synonyms
TB culture
AFB culture
tuberculosis
atypical mycobacteria
MAC
mycobacteriaum avium intercellulare complex
MAI
Collection
Sample Type
Sputum, BAL, bronchial wash
Collect
Urine cup or sterilecontainer
Amount to Collect
Sputum: 5 mL
BAL or Bronchial wash: 10 mL
Preferred Volume
Sputum: 5 mL
?BAL or Bronchial wash: 10 mL
Minimum Volume
Sputum: 1 mL
?BAL or Bronchial wash: 2 mL
Remarks
Sputum: Collect 3 separate sputum samples in 8-12 hour intervals, including at least one early morning sample.
Sputum samples collected < 8 hours apart are pooled & tested as a single specimen.
Stability (from collection to initiation)
Refrigerated 3 days.
Processing
Test Code
P285R
Test Group
Mycobacteria
Performing Lab
Microbiology
Specimen Preparation
Sputum: Before accessioning, check if a sample was submitted earlier. If earlier sample was collected < 8 hours apart from the sample just received, call China Basin to check if earlier sample has been processed.
1. If earlier sample has not been processed, TND as duplicate and enter code AFPOOL. Inform submitting location that the sample is being pooled with the prior sample, and to collect an additional sputum. Affix a note to the sample stating “Pool sample for AFB with (accession # of earlier sample)".
2. If earlier sample has been processed, TND as duplicate and freetext “Collect sputa for AFB culture 8 hours apart".
Bronchial lavage/wash: Aliquot the maximum amount of specimen available (>10 mL) into a blue top centrifuge tube.
Bronchial brush:
1. Use sterile forceps to transfer brush to a 50 ml blue-topped centrifuge tube.
2. Add NPC 67 Neutralizing buffer to make a total volume of 2 ml. 3. Vortex thoroughly to expel specimen from the brush and then remove brush from tube.
Preferred Volume
Sputum: 5 mL
?BAL or Bronchial wash: 10 mL
Minimum Volume
Sputum: 1 mL
?BAL or Bronchial wash: 2 mL
Stability (from collection to initiation)
Refrigerated 3 days.
Result Interpretation
Reference Interval
No AFB isolated
Critical Values
Inpatient results only. After hours outpatient results will be phoned the following morning.
First positive AFB smear, first positive AFB culture if smear negative or no smear, or first isolate of M. tuberculosis; Repeat call only for positive sample from different site or > 2 months since initial call.
Administrative
CPT Codes
87116, 87206
Complete View
Available Stat
No
Test Code
P285R
Test Group
Mycobacteria
Performing Lab
Microbiology
Performed
Daily, day shift
Methodology
Culture
Remarks
Sputum: Collect 3 separate sputum samples in 8-12 hour intervals, including at least one early morning sample.
Sputum samples collected < 8 hours apart are pooled & tested as a single specimen.
Collect
Urine cup or sterilecontainer
Amount to Collect
Sputum: 5 mL
BAL or Bronchial wash: 10 mL
Sample Type
Sputum, BAL, bronchial wash
Preferred Volume
Sputum: 5 mL
?BAL or Bronchial wash: 10 mL
Minimum Volume
Sputum: 1 mL
?BAL or Bronchial wash: 2 mL
Specimen Preparation
Sputum: Before accessioning, check if a sample was submitted earlier. If earlier sample was collected < 8 hours apart from the sample just received, call China Basin to check if earlier sample has been processed.
1. If earlier sample has not been processed, TND as duplicate and enter code AFPOOL. Inform submitting location that the sample is being pooled with the prior sample, and to collect an additional sputum. Affix a note to the sample stating “Pool sample for AFB with (accession # of earlier sample)".
2. If earlier sample has been processed, TND as duplicate and freetext “Collect sputa for AFB culture 8 hours apart".
Bronchial lavage/wash: Aliquot the maximum amount of specimen available (>10 mL) into a blue top centrifuge tube.
Bronchial brush:
1. Use sterile forceps to transfer brush to a 50 ml blue-topped centrifuge tube.
2. Add NPC 67 Neutralizing buffer to make a total volume of 2 ml. 3. Vortex thoroughly to expel specimen from the brush and then remove brush from tube.
Reference Interval
No AFB isolated
Critical Values
Inpatient results only. After hours outpatient results will be phoned the following morning.
First positive AFB smear, first positive AFB culture if smear negative or no smear, or first isolate of M. tuberculosis; Repeat call only for positive sample from different site or > 2 months since initial call.
Synonyms
TB culture
AFB culture
tuberculosis
atypical mycobacteria
MAC
mycobacteriaum avium intercellulare complex
MAI
Stability (from collection to initiation)
Refrigerated 3 days.
Reported
Up to 7 weeks
Reflex Testing
Smears will be performed on respiratory samples. Separate charges will be billed for AFB smear and AFB culture. Additional charges will be billed for decontamination of samples from nonsterile sites.
Mycobacterium tuberculosis complex DNA is automatically performed on respiratory specimens with positive smears, and billed separately.
