Available Stat

No

Performing Lab

Medical genomics - Cytogenetics

Performed

Set up daily, Monday - Friday

Methodology

Fluorescent in-situ hybridization (FISH)

Reported

2-3 days

Additional Information

Direct FISH is limited to the detection of aneuploidy (increase or decrease in copy number) of chromosomes 13, 18, 21, X and Y by analyzing INTERPHASE nuclei. A normal result indicates that no numeric abnormality of chromosomes 13, 18, 21, X and Y were identified. It does not rule out the possibility of structural defects or numeric defects in other chromosomes.

DIRECT FISH results are preliminary: G-banded chromosome analysis of 15 colonies or 20 cells remains the standard of care for prenatal diagnosis and all cytogenetic studies. It is also standard of care that no irreversible therapeutic action be initiated on the basis of DIRECT FISH results alone. About one third of all chromosome abnormalities cannot be detected by DIRECT FISH, including structural abnormalities, mosaicism, and numerical abnormalities of other chromosomes.

"Direct FISH" is the UCSF Cytogenetics name for interphase fluorescence in situ hybridization (FISH) analysis for chromosomes 13, 18, 21, X, and Y. 

Each FISH test is developed and its performance characteristics determined by the UCSF Cytogenetics Laboratory as required by CLIA '88 regulations. It has not been cleared or approved for specific uses by the U.S. Food and Drug Administration. All FISH probes undergo internal validation and quality control testing at UCSF Cytogenetics Lab prior to use.

Reflex Testing

If an abnormality is detected the Director will determine the appropriate additional studies to be performed to characterize the abnormality.

Additional testing may be omitted if specifically requested when the sample is submitted for cytogenetic analysis.

Synonyms

  • AV 18/XY
  • Direct aneuploidy FISH 18/XY
  • A1321
  • BA1321

Sample Type

Heparinized whole blood, Amniotic fluid, CVS, POC

Collect

Blood: Dark green top
Amniotic fluid: Sterile screw top container
CVS or POC: 15 mL centrifuge tube with transport media (Avaialable from Cytogenetics: 353-4844)

Preferred Volume

Whole blood, child or adult: 5 mL
Whole blood, infant: 2 mL
Amniotic fluid: 10 mL
CVS: 10 mg
POC: 10 mg

Minimum Volume

Whole blood, child or adult: 2 mL
Whole blood, infant: 1 mL
Amniotic fluid: 5 mL
CVS: 5 mg
POC: 5 mg

Stability (from collection to initiation)

1-2 days

Test Code

BA1321: Blood
A1321: Amniotic fluid, CVS

Test Group

FISH

Performing Lab

Medical genomics - Cytogenetics

Specimen Preparation

Keep samples at Room temperature. DO NOT CENTRIFUGE for any reason. Send all tubes and completed paperwork asap to the Cytogenetics laboratory at China Basin.

Preferred Volume

Whole blood, child or adult: 5 mL
Whole blood, infant: 2 mL
Amniotic fluid: 10 mL
CVS: 10 mg
POC: 10 mg

Minimum Volume

Whole blood, child or adult: 2 mL
Whole blood, infant: 1 mL
Amniotic fluid: 5 mL
CVS: 5 mg
POC: 5 mg

Stability (from collection to initiation)

1-2 days

Additional Information

Direct FISH is limited to the detection of aneuploidy (increase or decrease in copy number) of chromosomes 13, 18, 21, X and Y by analyzing INTERPHASE nuclei. A normal result indicates that no numeric abnormality of chromosomes 13, 18, 21, X and Y were identified. It does not rule out the possibility of structural defects or numeric defects in other chromosomes.

DIRECT FISH results are preliminary: G-banded chromosome analysis of 15 colonies or 20 cells remains the standard of care for prenatal diagnosis and all cytogenetic studies. It is also standard of care that no irreversible therapeutic action be initiated on the basis of DIRECT FISH results alone. About one third of all chromosome abnormalities cannot be detected by DIRECT FISH, including structural abnormalities, mosaicism, and numerical abnormalities of other chromosomes.

"Direct FISH" is the UCSF Cytogenetics name for interphase fluorescence in situ hybridization (FISH) analysis for chromosomes 13, 18, 21, X, and Y. 

Each FISH test is developed and its performance characteristics determined by the UCSF Cytogenetics Laboratory as required by CLIA '88 regulations. It has not been cleared or approved for specific uses by the U.S. Food and Drug Administration. All FISH probes undergo internal validation and quality control testing at UCSF Cytogenetics Lab prior to use.

CPT Codes

88271 x2, 88275, 88291

LDT or Modified FDA

Yes

Available Stat

No

Test Code

BA1321: Blood
A1321: Amniotic fluid, CVS

Test Group

FISH

Performing Lab

Medical genomics - Cytogenetics

Performed

Set up daily, Monday - Friday

Methodology

Fluorescent in-situ hybridization (FISH)

Collect

Blood: Dark green top
Amniotic fluid: Sterile screw top container
CVS or POC: 15 mL centrifuge tube with transport media (Avaialable from Cytogenetics: 353-4844)

Sample Type

Heparinized whole blood, Amniotic fluid, CVS, POC

Preferred Volume

Whole blood, child or adult: 5 mL
Whole blood, infant: 2 mL
Amniotic fluid: 10 mL
CVS: 10 mg
POC: 10 mg

Minimum Volume

Whole blood, child or adult: 2 mL
Whole blood, infant: 1 mL
Amniotic fluid: 5 mL
CVS: 5 mg
POC: 5 mg

Specimen Preparation

Keep samples at Room temperature. DO NOT CENTRIFUGE for any reason. Send all tubes and completed paperwork asap to the Cytogenetics laboratory at China Basin.

Synonyms

  • AV 18/XY
  • Direct aneuploidy FISH 18/XY
  • A1321
  • BA1321

Stability (from collection to initiation)

1-2 days

Reported

2-3 days

Reflex Testing

If an abnormality is detected the Director will determine the appropriate additional studies to be performed to characterize the abnormality.

Additional testing may be omitted if specifically requested when the sample is submitted for cytogenetic analysis.

Additional Information

Direct FISH is limited to the detection of aneuploidy (increase or decrease in copy number) of chromosomes 13, 18, 21, X and Y by analyzing INTERPHASE nuclei. A normal result indicates that no numeric abnormality of chromosomes 13, 18, 21, X and Y were identified. It does not rule out the possibility of structural defects or numeric defects in other chromosomes.

DIRECT FISH results are preliminary: G-banded chromosome analysis of 15 colonies or 20 cells remains the standard of care for prenatal diagnosis and all cytogenetic studies. It is also standard of care that no irreversible therapeutic action be initiated on the basis of DIRECT FISH results alone. About one third of all chromosome abnormalities cannot be detected by DIRECT FISH, including structural abnormalities, mosaicism, and numerical abnormalities of other chromosomes.

"Direct FISH" is the UCSF Cytogenetics name for interphase fluorescence in situ hybridization (FISH) analysis for chromosomes 13, 18, 21, X, and Y. 

Each FISH test is developed and its performance characteristics determined by the UCSF Cytogenetics Laboratory as required by CLIA '88 regulations. It has not been cleared or approved for specific uses by the U.S. Food and Drug Administration. All FISH probes undergo internal validation and quality control testing at UCSF Cytogenetics Lab prior to use.

CPT Codes

88271 x2, 88275, 88291

LDT or Modified FDA

Yes
Ordering

Available Stat

No

Performing Lab

Medical genomics - Cytogenetics

Performed

Set up daily, Monday - Friday

Methodology

Fluorescent in-situ hybridization (FISH)

Reported

2-3 days

Additional Information

Direct FISH is limited to the detection of aneuploidy (increase or decrease in copy number) of chromosomes 13, 18, 21, X and Y by analyzing INTERPHASE nuclei. A normal result indicates that no numeric abnormality of chromosomes 13, 18, 21, X and Y were identified. It does not rule out the possibility of structural defects or numeric defects in other chromosomes.

DIRECT FISH results are preliminary: G-banded chromosome analysis of 15 colonies or 20 cells remains the standard of care for prenatal diagnosis and all cytogenetic studies. It is also standard of care that no irreversible therapeutic action be initiated on the basis of DIRECT FISH results alone. About one third of all chromosome abnormalities cannot be detected by DIRECT FISH, including structural abnormalities, mosaicism, and numerical abnormalities of other chromosomes.

"Direct FISH" is the UCSF Cytogenetics name for interphase fluorescence in situ hybridization (FISH) analysis for chromosomes 13, 18, 21, X, and Y. 

Each FISH test is developed and its performance characteristics determined by the UCSF Cytogenetics Laboratory as required by CLIA '88 regulations. It has not been cleared or approved for specific uses by the U.S. Food and Drug Administration. All FISH probes undergo internal validation and quality control testing at UCSF Cytogenetics Lab prior to use.

Reflex Testing

If an abnormality is detected the Director will determine the appropriate additional studies to be performed to characterize the abnormality.

Additional testing may be omitted if specifically requested when the sample is submitted for cytogenetic analysis.

Synonyms

  • AV 18/XY
  • Direct aneuploidy FISH 18/XY
  • A1321
  • BA1321
Collection

Sample Type

Heparinized whole blood, Amniotic fluid, CVS, POC

Collect

Blood: Dark green top
Amniotic fluid: Sterile screw top container
CVS or POC: 15 mL centrifuge tube with transport media (Avaialable from Cytogenetics: 353-4844)

Preferred Volume

Whole blood, child or adult: 5 mL
Whole blood, infant: 2 mL
Amniotic fluid: 10 mL
CVS: 10 mg
POC: 10 mg

Minimum Volume

Whole blood, child or adult: 2 mL
Whole blood, infant: 1 mL
Amniotic fluid: 5 mL
CVS: 5 mg
POC: 5 mg

Stability (from collection to initiation)

1-2 days
Processing

Test Code

BA1321: Blood
A1321: Amniotic fluid, CVS

Test Group

FISH

Performing Lab

Medical genomics - Cytogenetics

Specimen Preparation

Keep samples at Room temperature. DO NOT CENTRIFUGE for any reason. Send all tubes and completed paperwork asap to the Cytogenetics laboratory at China Basin.

Preferred Volume

Whole blood, child or adult: 5 mL
Whole blood, infant: 2 mL
Amniotic fluid: 10 mL
CVS: 10 mg
POC: 10 mg

Minimum Volume

Whole blood, child or adult: 2 mL
Whole blood, infant: 1 mL
Amniotic fluid: 5 mL
CVS: 5 mg
POC: 5 mg

Stability (from collection to initiation)

1-2 days
Result Interpretation

Additional Information

Direct FISH is limited to the detection of aneuploidy (increase or decrease in copy number) of chromosomes 13, 18, 21, X and Y by analyzing INTERPHASE nuclei. A normal result indicates that no numeric abnormality of chromosomes 13, 18, 21, X and Y were identified. It does not rule out the possibility of structural defects or numeric defects in other chromosomes.

DIRECT FISH results are preliminary: G-banded chromosome analysis of 15 colonies or 20 cells remains the standard of care for prenatal diagnosis and all cytogenetic studies. It is also standard of care that no irreversible therapeutic action be initiated on the basis of DIRECT FISH results alone. About one third of all chromosome abnormalities cannot be detected by DIRECT FISH, including structural abnormalities, mosaicism, and numerical abnormalities of other chromosomes.

"Direct FISH" is the UCSF Cytogenetics name for interphase fluorescence in situ hybridization (FISH) analysis for chromosomes 13, 18, 21, X, and Y. 

Each FISH test is developed and its performance characteristics determined by the UCSF Cytogenetics Laboratory as required by CLIA '88 regulations. It has not been cleared or approved for specific uses by the U.S. Food and Drug Administration. All FISH probes undergo internal validation and quality control testing at UCSF Cytogenetics Lab prior to use.
Administrative

CPT Codes

88271 x2, 88275, 88291

LDT or Modified FDA

Yes
Complete View

Available Stat

No

Test Code

BA1321: Blood
A1321: Amniotic fluid, CVS

Test Group

FISH

Performing Lab

Medical genomics - Cytogenetics

Performed

Set up daily, Monday - Friday

Methodology

Fluorescent in-situ hybridization (FISH)

Collect

Blood: Dark green top
Amniotic fluid: Sterile screw top container
CVS or POC: 15 mL centrifuge tube with transport media (Avaialable from Cytogenetics: 353-4844)

Sample Type

Heparinized whole blood, Amniotic fluid, CVS, POC

Preferred Volume

Whole blood, child or adult: 5 mL
Whole blood, infant: 2 mL
Amniotic fluid: 10 mL
CVS: 10 mg
POC: 10 mg

Minimum Volume

Whole blood, child or adult: 2 mL
Whole blood, infant: 1 mL
Amniotic fluid: 5 mL
CVS: 5 mg
POC: 5 mg

Specimen Preparation

Keep samples at Room temperature. DO NOT CENTRIFUGE for any reason. Send all tubes and completed paperwork asap to the Cytogenetics laboratory at China Basin.

Synonyms

  • AV 18/XY
  • Direct aneuploidy FISH 18/XY
  • A1321
  • BA1321

Stability (from collection to initiation)

1-2 days

Reported

2-3 days

Reflex Testing

If an abnormality is detected the Director will determine the appropriate additional studies to be performed to characterize the abnormality.

Additional testing may be omitted if specifically requested when the sample is submitted for cytogenetic analysis.

Additional Information

Direct FISH is limited to the detection of aneuploidy (increase or decrease in copy number) of chromosomes 13, 18, 21, X and Y by analyzing INTERPHASE nuclei. A normal result indicates that no numeric abnormality of chromosomes 13, 18, 21, X and Y were identified. It does not rule out the possibility of structural defects or numeric defects in other chromosomes.

DIRECT FISH results are preliminary: G-banded chromosome analysis of 15 colonies or 20 cells remains the standard of care for prenatal diagnosis and all cytogenetic studies. It is also standard of care that no irreversible therapeutic action be initiated on the basis of DIRECT FISH results alone. About one third of all chromosome abnormalities cannot be detected by DIRECT FISH, including structural abnormalities, mosaicism, and numerical abnormalities of other chromosomes.

"Direct FISH" is the UCSF Cytogenetics name for interphase fluorescence in situ hybridization (FISH) analysis for chromosomes 13, 18, 21, X, and Y. 

Each FISH test is developed and its performance characteristics determined by the UCSF Cytogenetics Laboratory as required by CLIA '88 regulations. It has not been cleared or approved for specific uses by the U.S. Food and Drug Administration. All FISH probes undergo internal validation and quality control testing at UCSF Cytogenetics Lab prior to use.

CPT Codes

88271 x2, 88275, 88291

LDT or Modified FDA

Yes