Approval Required

Yes, testing of UCSF patients requires approval by Microbiology in consultation with Infectious Disease or Neurology. UCSF outpatient testing requires insurance preauthorization.  Please follow this link for an insurance preauthorization template (Microsoft Word format).

Requests from outside UCSF require an institutional account prior to sample submission. See https://nextgendiagnostics.ucsf.edu for details.

Note, CSF is the only sample type accepted from outside UCSF.

 In select cases, plasma samples may be sent to Karius for testing (see 'Additional Information' below).

Available Stat

No

Performing Lab

Microbiology

Methodology

mNGS

Reported

1-2 weeks

Additional Information

Note, CSF is the only sample type accepted from outside UCSF

Test is interpreted by the Microbiology lab director and discussed with treating physicians as part of the Clinical Microbial Sequencing Board.

See
http://nextgendiagnostics.ucsf.edu for additional information.

With approval from the Microbiology lab, plasma samples in select cases may be sent to Karius for testing.  Order as "Microbiology - Test Not Listed (Special Sendout Test) (aka P319)."

For Karius collection and processing instructions, click here.

Synonyms

  • Metagenomic next-generation sequencing, deep sequencing

Sample Type

CSF, EDTA plasma (EDTA whole blood must be centrifuged and plasma separated from red cells within 6 hours of collection). 

Collect

CSF: Sterile black-top tube
Plasma: Lavender or pearl top

Preferred Volume

2ml

Minimum Volume

1ml

Remarks

Lumbar puncture (CSF)

Stability (from collection to initiation)

CSF: Room temperature 6 hours or refrigerated 6 days, but prefer to freeze within 6 hours; stable frozen at -25C or -70C for 1 month.

Plasma: Room temperature up to 6 hours for whole blood; plasma frozen at -70C for 1 month.

Unacceptable Conditions

Samples submitted in incorrect tube, grossly hemolyzed samples

Test Code

P710

Performing Lab

Microbiology

Specimen Preparation

UCSF: Freeze at -70ºC on receipt at China Basin. Other sites: Freeze CSF and ship to China Basin microbiology. Aliquot (if necessary) only under sterile conditions.

Additional Processing Instructions

With approval from the Microbiology lab, plasma samples in select cases may be sent to Karius for testing.  Order as "Microbiology - Test Not Listed (Special Sendout Test) (aka P319)." 

​For Karius collection and processing instructions, click here.

Preferred Volume

2ml

Minimum Volume

1ml

Unacceptable Conditions

Samples submitted in incorrect tube, grossly hemolyzed samples

Stability (from collection to initiation)

CSF: Room temperature 6 hours or refrigerated 6 days, but prefer to freeze within 6 hours; stable frozen at -25C or -70C for 1 month.

Plasma: Room temperature up to 6 hours for whole blood; plasma frozen at -70C for 1 month.

Reference Interval

No microorganisms detected

Additional Information

Note, CSF is the only sample type accepted from outside UCSF

Test is interpreted by the Microbiology lab director and discussed with treating physicians as part of the Clinical Microbial Sequencing Board.

See
http://nextgendiagnostics.ucsf.edu for additional information.

With approval from the Microbiology lab, plasma samples in select cases may be sent to Karius for testing.  Order as "Microbiology - Test Not Listed (Special Sendout Test) (aka P319)."

For Karius collection and processing instructions, click here.

CPT Codes

81479

LDT or Modified FDA

Yes

Approval Required

Yes, testing of UCSF patients requires approval by Microbiology in consultation with Infectious Disease or Neurology. UCSF outpatient testing requires insurance preauthorization.  Please follow this link for an insurance preauthorization template (Microsoft Word format).

Requests from outside UCSF require an institutional account prior to sample submission. See https://nextgendiagnostics.ucsf.edu for details.

Note, CSF is the only sample type accepted from outside UCSF.

 In select cases, plasma samples may be sent to Karius for testing (see 'Additional Information' below).

Available Stat

No

Test Code

P710

Performing Lab

Microbiology

Methodology

mNGS

Remarks

Lumbar puncture (CSF)

Collect

CSF: Sterile black-top tube
Plasma: Lavender or pearl top

Sample Type

CSF, EDTA plasma (EDTA whole blood must be centrifuged and plasma separated from red cells within 6 hours of collection). 

Preferred Volume

2ml

Minimum Volume

1ml

Unacceptable Conditions

Samples submitted in incorrect tube, grossly hemolyzed samples

Specimen Preparation

UCSF: Freeze at -70ºC on receipt at China Basin. Other sites: Freeze CSF and ship to China Basin microbiology. Aliquot (if necessary) only under sterile conditions.

Additional Processing Instructions

With approval from the Microbiology lab, plasma samples in select cases may be sent to Karius for testing.  Order as "Microbiology - Test Not Listed (Special Sendout Test) (aka P319)." 

​For Karius collection and processing instructions, click here.

Reference Interval

No microorganisms detected

Synonyms

  • Metagenomic next-generation sequencing, deep sequencing

Stability (from collection to initiation)

CSF: Room temperature 6 hours or refrigerated 6 days, but prefer to freeze within 6 hours; stable frozen at -25C or -70C for 1 month.

Plasma: Room temperature up to 6 hours for whole blood; plasma frozen at -70C for 1 month.

Reported

1-2 weeks

Additional Information

Note, CSF is the only sample type accepted from outside UCSF

Test is interpreted by the Microbiology lab director and discussed with treating physicians as part of the Clinical Microbial Sequencing Board.

See
http://nextgendiagnostics.ucsf.edu for additional information.

With approval from the Microbiology lab, plasma samples in select cases may be sent to Karius for testing.  Order as "Microbiology - Test Not Listed (Special Sendout Test) (aka P319)."

For Karius collection and processing instructions, click here.

CPT Codes

81479

LDT or Modified FDA

Yes
Ordering

Approval Required

Yes, testing of UCSF patients requires approval by Microbiology in consultation with Infectious Disease or Neurology. UCSF outpatient testing requires insurance preauthorization.  Please follow this link for an insurance preauthorization template (Microsoft Word format).

Requests from outside UCSF require an institutional account prior to sample submission. See https://nextgendiagnostics.ucsf.edu for details.

Note, CSF is the only sample type accepted from outside UCSF.

 In select cases, plasma samples may be sent to Karius for testing (see 'Additional Information' below).

Available Stat

No

Performing Lab

Microbiology

Methodology

mNGS

Reported

1-2 weeks

Additional Information

Note, CSF is the only sample type accepted from outside UCSF

Test is interpreted by the Microbiology lab director and discussed with treating physicians as part of the Clinical Microbial Sequencing Board.

See
http://nextgendiagnostics.ucsf.edu for additional information.

With approval from the Microbiology lab, plasma samples in select cases may be sent to Karius for testing.  Order as "Microbiology - Test Not Listed (Special Sendout Test) (aka P319)."

For Karius collection and processing instructions, click here.

Synonyms

  • Metagenomic next-generation sequencing, deep sequencing
Collection

Sample Type

CSF, EDTA plasma (EDTA whole blood must be centrifuged and plasma separated from red cells within 6 hours of collection). 

Collect

CSF: Sterile black-top tube
Plasma: Lavender or pearl top

Preferred Volume

2ml

Minimum Volume

1ml

Remarks

Lumbar puncture (CSF)

Stability (from collection to initiation)

CSF: Room temperature 6 hours or refrigerated 6 days, but prefer to freeze within 6 hours; stable frozen at -25C or -70C for 1 month.

Plasma: Room temperature up to 6 hours for whole blood; plasma frozen at -70C for 1 month.

Unacceptable Conditions

Samples submitted in incorrect tube, grossly hemolyzed samples
Processing

Test Code

P710

Performing Lab

Microbiology

Specimen Preparation

UCSF: Freeze at -70ºC on receipt at China Basin. Other sites: Freeze CSF and ship to China Basin microbiology. Aliquot (if necessary) only under sterile conditions.

Additional Processing Instructions

With approval from the Microbiology lab, plasma samples in select cases may be sent to Karius for testing.  Order as "Microbiology - Test Not Listed (Special Sendout Test) (aka P319)." 

​For Karius collection and processing instructions, click here.

Preferred Volume

2ml

Minimum Volume

1ml

Unacceptable Conditions

Samples submitted in incorrect tube, grossly hemolyzed samples

Stability (from collection to initiation)

CSF: Room temperature 6 hours or refrigerated 6 days, but prefer to freeze within 6 hours; stable frozen at -25C or -70C for 1 month.

Plasma: Room temperature up to 6 hours for whole blood; plasma frozen at -70C for 1 month.
Result Interpretation

Reference Interval

No microorganisms detected

Additional Information

Note, CSF is the only sample type accepted from outside UCSF

Test is interpreted by the Microbiology lab director and discussed with treating physicians as part of the Clinical Microbial Sequencing Board.

See
http://nextgendiagnostics.ucsf.edu for additional information.

With approval from the Microbiology lab, plasma samples in select cases may be sent to Karius for testing.  Order as "Microbiology - Test Not Listed (Special Sendout Test) (aka P319)."

For Karius collection and processing instructions, click here.
Administrative

CPT Codes

81479

LDT or Modified FDA

Yes
Complete View

Approval Required

Yes, testing of UCSF patients requires approval by Microbiology in consultation with Infectious Disease or Neurology. UCSF outpatient testing requires insurance preauthorization.  Please follow this link for an insurance preauthorization template (Microsoft Word format).

Requests from outside UCSF require an institutional account prior to sample submission. See https://nextgendiagnostics.ucsf.edu for details.

Note, CSF is the only sample type accepted from outside UCSF.

 In select cases, plasma samples may be sent to Karius for testing (see 'Additional Information' below).

Available Stat

No

Test Code

P710

Performing Lab

Microbiology

Methodology

mNGS

Remarks

Lumbar puncture (CSF)

Collect

CSF: Sterile black-top tube
Plasma: Lavender or pearl top

Sample Type

CSF, EDTA plasma (EDTA whole blood must be centrifuged and plasma separated from red cells within 6 hours of collection). 

Preferred Volume

2ml

Minimum Volume

1ml

Unacceptable Conditions

Samples submitted in incorrect tube, grossly hemolyzed samples

Specimen Preparation

UCSF: Freeze at -70ºC on receipt at China Basin. Other sites: Freeze CSF and ship to China Basin microbiology. Aliquot (if necessary) only under sterile conditions.

Additional Processing Instructions

With approval from the Microbiology lab, plasma samples in select cases may be sent to Karius for testing.  Order as "Microbiology - Test Not Listed (Special Sendout Test) (aka P319)." 

​For Karius collection and processing instructions, click here.

Reference Interval

No microorganisms detected

Synonyms

  • Metagenomic next-generation sequencing, deep sequencing

Stability (from collection to initiation)

CSF: Room temperature 6 hours or refrigerated 6 days, but prefer to freeze within 6 hours; stable frozen at -25C or -70C for 1 month.

Plasma: Room temperature up to 6 hours for whole blood; plasma frozen at -70C for 1 month.

Reported

1-2 weeks

Additional Information

Note, CSF is the only sample type accepted from outside UCSF

Test is interpreted by the Microbiology lab director and discussed with treating physicians as part of the Clinical Microbial Sequencing Board.

See
http://nextgendiagnostics.ucsf.edu for additional information.

With approval from the Microbiology lab, plasma samples in select cases may be sent to Karius for testing.  Order as "Microbiology - Test Not Listed (Special Sendout Test) (aka P319)."

For Karius collection and processing instructions, click here.

CPT Codes

81479

LDT or Modified FDA

Yes