Yes, testing of UCSF patients requires approval by Microbiology in consultation with Neurology (UCSF CSF mNGS) or Infectious Disease (UCSF plasma mNGS).
Sendout testing of UCSF patients to Delve Bio for CSF mNGS requires advance Neurology approval.
Outreach testing for non-UC patients by UCSF and clinical mNGS testing of body fluids and tissues other than CSF / plasma are not currently available at this time.
Available Stat
No
Performing Lab
Microbiology
Performed
Weekdays
Methodology
mNGS
Reported
1-2 weeks
Additional Information
Test results are interpreted by the Microbiology lab director and may be discussed with treating physicians as part of an ad hoc Clinical Microbial Sequencing Board (CMSB).
For inquiries, please call the Clinical Microbiology Lab at 415-353-1268.
Synonyms
metagenomic next-generation sequencing
mNGS
agnostic sequencing
untargeted sequencing
mNGS Path Dx
Sample Type
CSF, EDTA plasma (EDTA whole blood must be centrifuged and plasma separated from red cells within 6 hours of collection).
Collect
CSF: Sterile tube
Plasma: Lavender top tube. A dedicated tube is strongly preferred.
Preferred Volume
2ml
Minimum Volume
1ml
Stability (from collection to initiation)
CSF: Room temperature 6 hours or refrigerated 6 days but prefer to freeze within 6 hours; stable frozen at -25C or -70C for up to 1 year.
Plasma: Room temperature up to 6 hours for whole blood prior to centrifugation; plasma refrigerated up to 7 days; stable frozen at -25C or -70C for up to 1 year.
Unacceptable Conditions
Samples submitted in incorrect tube, grossly hemolyzed samples
Performing Lab
Microbiology
Specimen Preparation
UCSF: Freeze at -70ºC on receipt at China Basin. Other sites: Freeze sample and ship to China Basin microbiology. Aliquot (if necessary) only under sterile conditions.
Preferred Volume
2ml
Minimum Volume
1ml
Unacceptable Conditions
Samples submitted in incorrect tube, grossly hemolyzed samples
Stability (from collection to initiation)
CSF: Room temperature 6 hours or refrigerated 6 days but prefer to freeze within 6 hours; stable frozen at -25C or -70C for up to 1 year.
Plasma: Room temperature up to 6 hours for whole blood prior to centrifugation; plasma refrigerated up to 7 days; stable frozen at -25C or -70C for up to 1 year.
Reference Interval
No microorganisms detected
Additional Information
Test results are interpreted by the Microbiology lab director and may be discussed with treating physicians as part of an ad hoc Clinical Microbial Sequencing Board (CMSB).
For inquiries, please call the Clinical Microbiology Lab at 415-353-1268.
CPT Codes
87999
LDT or Modified FDA
Yes
Approval Required
Yes, testing of UCSF patients requires approval by Microbiology in consultation with Neurology (UCSF CSF mNGS) or Infectious Disease (UCSF plasma mNGS).
Sendout testing of UCSF patients to Delve Bio for CSF mNGS requires advance Neurology approval.
Outreach testing for non-UC patients by UCSF and clinical mNGS testing of body fluids and tissues other than CSF / plasma are not currently available at this time.
Available Stat
No
Performing Lab
Microbiology
Performed
Weekdays
Methodology
mNGS
Collect
CSF: Sterile tube
Plasma: Lavender top tube. A dedicated tube is strongly preferred.
Sample Type
CSF, EDTA plasma (EDTA whole blood must be centrifuged and plasma separated from red cells within 6 hours of collection).
Preferred Volume
2ml
Minimum Volume
1ml
Unacceptable Conditions
Samples submitted in incorrect tube, grossly hemolyzed samples
Specimen Preparation
UCSF: Freeze at -70ºC on receipt at China Basin. Other sites: Freeze sample and ship to China Basin microbiology. Aliquot (if necessary) only under sterile conditions.
Reference Interval
No microorganisms detected
Synonyms
metagenomic next-generation sequencing
mNGS
agnostic sequencing
untargeted sequencing
mNGS Path Dx
Stability (from collection to initiation)
CSF: Room temperature 6 hours or refrigerated 6 days but prefer to freeze within 6 hours; stable frozen at -25C or -70C for up to 1 year.
Plasma: Room temperature up to 6 hours for whole blood prior to centrifugation; plasma refrigerated up to 7 days; stable frozen at -25C or -70C for up to 1 year.
Reported
1-2 weeks
Additional Information
Test results are interpreted by the Microbiology lab director and may be discussed with treating physicians as part of an ad hoc Clinical Microbial Sequencing Board (CMSB).
For inquiries, please call the Clinical Microbiology Lab at 415-353-1268.
CPT Codes
87999
LDT or Modified FDA
Yes
Ordering
Approval Required
Yes, testing of UCSF patients requires approval by Microbiology in consultation with Neurology (UCSF CSF mNGS) or Infectious Disease (UCSF plasma mNGS).
Sendout testing of UCSF patients to Delve Bio for CSF mNGS requires advance Neurology approval.
Outreach testing for non-UC patients by UCSF and clinical mNGS testing of body fluids and tissues other than CSF / plasma are not currently available at this time.
Available Stat
No
Performing Lab
Microbiology
Performed
Weekdays
Methodology
mNGS
Reported
1-2 weeks
Additional Information
Test results are interpreted by the Microbiology lab director and may be discussed with treating physicians as part of an ad hoc Clinical Microbial Sequencing Board (CMSB).
For inquiries, please call the Clinical Microbiology Lab at 415-353-1268.
Synonyms
metagenomic next-generation sequencing
mNGS
agnostic sequencing
untargeted sequencing
mNGS Path Dx
Collection
Sample Type
CSF, EDTA plasma (EDTA whole blood must be centrifuged and plasma separated from red cells within 6 hours of collection).
Collect
CSF: Sterile tube
Plasma: Lavender top tube. A dedicated tube is strongly preferred.
Preferred Volume
2ml
Minimum Volume
1ml
Stability (from collection to initiation)
CSF: Room temperature 6 hours or refrigerated 6 days but prefer to freeze within 6 hours; stable frozen at -25C or -70C for up to 1 year.
Plasma: Room temperature up to 6 hours for whole blood prior to centrifugation; plasma refrigerated up to 7 days; stable frozen at -25C or -70C for up to 1 year.
Unacceptable Conditions
Samples submitted in incorrect tube, grossly hemolyzed samples
Processing
Performing Lab
Microbiology
Specimen Preparation
UCSF: Freeze at -70ºC on receipt at China Basin. Other sites: Freeze sample and ship to China Basin microbiology. Aliquot (if necessary) only under sterile conditions.
Preferred Volume
2ml
Minimum Volume
1ml
Unacceptable Conditions
Samples submitted in incorrect tube, grossly hemolyzed samples
Stability (from collection to initiation)
CSF: Room temperature 6 hours or refrigerated 6 days but prefer to freeze within 6 hours; stable frozen at -25C or -70C for up to 1 year.
Plasma: Room temperature up to 6 hours for whole blood prior to centrifugation; plasma refrigerated up to 7 days; stable frozen at -25C or -70C for up to 1 year.
Result Interpretation
Reference Interval
No microorganisms detected
Additional Information
Test results are interpreted by the Microbiology lab director and may be discussed with treating physicians as part of an ad hoc Clinical Microbial Sequencing Board (CMSB).
For inquiries, please call the Clinical Microbiology Lab at 415-353-1268.
Administrative
CPT Codes
87999
LDT or Modified FDA
Yes
Complete View
Approval Required
Yes, testing of UCSF patients requires approval by Microbiology in consultation with Neurology (UCSF CSF mNGS) or Infectious Disease (UCSF plasma mNGS).
Sendout testing of UCSF patients to Delve Bio for CSF mNGS requires advance Neurology approval.
Outreach testing for non-UC patients by UCSF and clinical mNGS testing of body fluids and tissues other than CSF / plasma are not currently available at this time.
Available Stat
No
Performing Lab
Microbiology
Performed
Weekdays
Methodology
mNGS
Collect
CSF: Sterile tube
Plasma: Lavender top tube. A dedicated tube is strongly preferred.
Sample Type
CSF, EDTA plasma (EDTA whole blood must be centrifuged and plasma separated from red cells within 6 hours of collection).
Preferred Volume
2ml
Minimum Volume
1ml
Unacceptable Conditions
Samples submitted in incorrect tube, grossly hemolyzed samples
Specimen Preparation
UCSF: Freeze at -70ºC on receipt at China Basin. Other sites: Freeze sample and ship to China Basin microbiology. Aliquot (if necessary) only under sterile conditions.
Reference Interval
No microorganisms detected
Synonyms
metagenomic next-generation sequencing
mNGS
agnostic sequencing
untargeted sequencing
mNGS Path Dx
Stability (from collection to initiation)
CSF: Room temperature 6 hours or refrigerated 6 days but prefer to freeze within 6 hours; stable frozen at -25C or -70C for up to 1 year.
Plasma: Room temperature up to 6 hours for whole blood prior to centrifugation; plasma refrigerated up to 7 days; stable frozen at -25C or -70C for up to 1 year.
Reported
1-2 weeks
Additional Information
Test results are interpreted by the Microbiology lab director and may be discussed with treating physicians as part of an ad hoc Clinical Microbial Sequencing Board (CMSB).
For inquiries, please call the Clinical Microbiology Lab at 415-353-1268.
