Yes, testing of UCSF patients requires approval by Microbiology in consultation with Infectious Disease or Neurology. UCSF outpatient testing requires insurance preauthorization. Please follow this link for an insurance preauthorization template (Microsoft Word format).
Requests from outside UCSF require an institutional account prior to sample submission. See https://nextgendiagnostics.ucsf.edu for details.
In select cases, plasma samples may be sent to Karius for testing (see 'Additional Information' below).
Available Stat
No
Performing Lab
Microbiology
Methodology
mNGS
Reported
1-2 weeks
Additional Information
Note, CSF is the only sample type accepted from outside UCSF
Test is interpreted by the Microbiology lab director and discussed with treating physicians as part of the Clinical Microbial Sequencing Board.
With approval from the Microbiology lab, plasma samples in select cases may be sent to Karius for testing. Order as "Microbiology - Test Not Listed (Special Sendout Test) (aka P319)."
For Karius collection and processing instructions, click here.
Synonyms
Metagenomic next-generation sequencing, deep sequencing
Sample Type
CSF, EDTA plasma (EDTA whole blood must be centrifuged and plasma separated from red cells within 6 hours of collection).
Collect
CSF: Sterile black-top tube
Plasma: Lavender or pearl top. Needs a dedicated tube. A shared sample is unacceptable.
Preferred Volume
2ml
Minimum Volume
1ml
Remarks
Lumbar puncture (CSF)
Stability (from collection to initiation)
CSF: Room temperature 6 hours or refrigerated 6 days, but prefer to freeze within 6 hours; stable frozen at -25C or -70C for 1 month.
Plasma: Room temperature up to 6 hours for whole blood; plasma frozen at -25C or -70C for 1 month.
Unacceptable Conditions
Samples submitted in incorrect tube, grossly hemolyzed samples, plasma sample shared with other tests.
Test Code
P710
Performing Lab
Microbiology
Specimen Preparation
UCSF: Freeze at -70ºC on receipt at China Basin.
Other sites: Freeze CSF and ship to China Basin microbiology. Aliquot (if necessary) only under sterile conditions.
Additional Processing Instructions
With approval from the Microbiology lab, plasma samples in select cases may be sent to Karius for testing. Order as "Microbiology - Test Not Listed (Special Sendout Test) (aka P319)."
For Karius collection and processing instructions, click here.
Preferred Volume
2ml
Minimum Volume
1ml
Unacceptable Conditions
Samples submitted in incorrect tube, grossly hemolyzed samples, plasma sample shared with other tests.
Stability (from collection to initiation)
CSF: Room temperature 6 hours or refrigerated 6 days, but prefer to freeze within 6 hours; stable frozen at -25C or -70C for 1 month.
Plasma: Room temperature up to 6 hours for whole blood; plasma frozen at -25C or -70C for 1 month.
Reference Interval
No microorganisms detected
Additional Information
Note, CSF is the only sample type accepted from outside UCSF
Test is interpreted by the Microbiology lab director and discussed with treating physicians as part of the Clinical Microbial Sequencing Board.
With approval from the Microbiology lab, plasma samples in select cases may be sent to Karius for testing. Order as "Microbiology - Test Not Listed (Special Sendout Test) (aka P319)."
For Karius collection and processing instructions, click here.
CPT Codes
81479
LDT or Modified FDA
Yes
Approval Required
Yes, testing of UCSF patients requires approval by Microbiology in consultation with Infectious Disease or Neurology. UCSF outpatient testing requires insurance preauthorization. Please follow this link for an insurance preauthorization template (Microsoft Word format).
Requests from outside UCSF require an institutional account prior to sample submission. See https://nextgendiagnostics.ucsf.edu for details.
In select cases, plasma samples may be sent to Karius for testing (see 'Additional Information' below).
Available Stat
No
Test Code
P710
Performing Lab
Microbiology
Methodology
mNGS
Remarks
Lumbar puncture (CSF)
Collect
CSF: Sterile black-top tube
Plasma: Lavender or pearl top. Needs a dedicated tube. A shared sample is unacceptable.
Sample Type
CSF, EDTA plasma (EDTA whole blood must be centrifuged and plasma separated from red cells within 6 hours of collection).
Preferred Volume
2ml
Minimum Volume
1ml
Unacceptable Conditions
Samples submitted in incorrect tube, grossly hemolyzed samples, plasma sample shared with other tests.
Specimen Preparation
UCSF: Freeze at -70ºC on receipt at China Basin.
Other sites: Freeze CSF and ship to China Basin microbiology. Aliquot (if necessary) only under sterile conditions.
Additional Processing Instructions
With approval from the Microbiology lab, plasma samples in select cases may be sent to Karius for testing. Order as "Microbiology - Test Not Listed (Special Sendout Test) (aka P319)."
For Karius collection and processing instructions, click here.
Reference Interval
No microorganisms detected
Synonyms
Metagenomic next-generation sequencing, deep sequencing
Stability (from collection to initiation)
CSF: Room temperature 6 hours or refrigerated 6 days, but prefer to freeze within 6 hours; stable frozen at -25C or -70C for 1 month.
Plasma: Room temperature up to 6 hours for whole blood; plasma frozen at -25C or -70C for 1 month.
Reported
1-2 weeks
Additional Information
Note, CSF is the only sample type accepted from outside UCSF
Test is interpreted by the Microbiology lab director and discussed with treating physicians as part of the Clinical Microbial Sequencing Board.
With approval from the Microbiology lab, plasma samples in select cases may be sent to Karius for testing. Order as "Microbiology - Test Not Listed (Special Sendout Test) (aka P319)."
For Karius collection and processing instructions, click here.
CPT Codes
81479
LDT or Modified FDA
Yes
Ordering
Approval Required
Yes, testing of UCSF patients requires approval by Microbiology in consultation with Infectious Disease or Neurology. UCSF outpatient testing requires insurance preauthorization. Please follow this link for an insurance preauthorization template (Microsoft Word format).
Requests from outside UCSF require an institutional account prior to sample submission. See https://nextgendiagnostics.ucsf.edu for details.
In select cases, plasma samples may be sent to Karius for testing (see 'Additional Information' below).
Available Stat
No
Performing Lab
Microbiology
Methodology
mNGS
Reported
1-2 weeks
Additional Information
Note, CSF is the only sample type accepted from outside UCSF
Test is interpreted by the Microbiology lab director and discussed with treating physicians as part of the Clinical Microbial Sequencing Board.
With approval from the Microbiology lab, plasma samples in select cases may be sent to Karius for testing. Order as "Microbiology - Test Not Listed (Special Sendout Test) (aka P319)."
For Karius collection and processing instructions, click here.
Synonyms
Metagenomic next-generation sequencing, deep sequencing
Collection
Sample Type
CSF, EDTA plasma (EDTA whole blood must be centrifuged and plasma separated from red cells within 6 hours of collection).
Collect
CSF: Sterile black-top tube
Plasma: Lavender or pearl top. Needs a dedicated tube. A shared sample is unacceptable.
Preferred Volume
2ml
Minimum Volume
1ml
Remarks
Lumbar puncture (CSF)
Stability (from collection to initiation)
CSF: Room temperature 6 hours or refrigerated 6 days, but prefer to freeze within 6 hours; stable frozen at -25C or -70C for 1 month.
Plasma: Room temperature up to 6 hours for whole blood; plasma frozen at -25C or -70C for 1 month.
Unacceptable Conditions
Samples submitted in incorrect tube, grossly hemolyzed samples, plasma sample shared with other tests.
Processing
Test Code
P710
Performing Lab
Microbiology
Specimen Preparation
UCSF: Freeze at -70ºC on receipt at China Basin.
Other sites: Freeze CSF and ship to China Basin microbiology. Aliquot (if necessary) only under sterile conditions.
Additional Processing Instructions
With approval from the Microbiology lab, plasma samples in select cases may be sent to Karius for testing. Order as "Microbiology - Test Not Listed (Special Sendout Test) (aka P319)."
For Karius collection and processing instructions, click here.
Preferred Volume
2ml
Minimum Volume
1ml
Unacceptable Conditions
Samples submitted in incorrect tube, grossly hemolyzed samples, plasma sample shared with other tests.
Stability (from collection to initiation)
CSF: Room temperature 6 hours or refrigerated 6 days, but prefer to freeze within 6 hours; stable frozen at -25C or -70C for 1 month.
Plasma: Room temperature up to 6 hours for whole blood; plasma frozen at -25C or -70C for 1 month.
Result Interpretation
Reference Interval
No microorganisms detected
Additional Information
Note, CSF is the only sample type accepted from outside UCSF
Test is interpreted by the Microbiology lab director and discussed with treating physicians as part of the Clinical Microbial Sequencing Board.
With approval from the Microbiology lab, plasma samples in select cases may be sent to Karius for testing. Order as "Microbiology - Test Not Listed (Special Sendout Test) (aka P319)."
For Karius collection and processing instructions, click here.
Administrative
CPT Codes
81479
LDT or Modified FDA
Yes
Complete View
Approval Required
Yes, testing of UCSF patients requires approval by Microbiology in consultation with Infectious Disease or Neurology. UCSF outpatient testing requires insurance preauthorization. Please follow this link for an insurance preauthorization template (Microsoft Word format).
Requests from outside UCSF require an institutional account prior to sample submission. See https://nextgendiagnostics.ucsf.edu for details.
In select cases, plasma samples may be sent to Karius for testing (see 'Additional Information' below).
Available Stat
No
Test Code
P710
Performing Lab
Microbiology
Methodology
mNGS
Remarks
Lumbar puncture (CSF)
Collect
CSF: Sterile black-top tube
Plasma: Lavender or pearl top. Needs a dedicated tube. A shared sample is unacceptable.
Sample Type
CSF, EDTA plasma (EDTA whole blood must be centrifuged and plasma separated from red cells within 6 hours of collection).
Preferred Volume
2ml
Minimum Volume
1ml
Unacceptable Conditions
Samples submitted in incorrect tube, grossly hemolyzed samples, plasma sample shared with other tests.
Specimen Preparation
UCSF: Freeze at -70ºC on receipt at China Basin.
Other sites: Freeze CSF and ship to China Basin microbiology. Aliquot (if necessary) only under sterile conditions.
Additional Processing Instructions
With approval from the Microbiology lab, plasma samples in select cases may be sent to Karius for testing. Order as "Microbiology - Test Not Listed (Special Sendout Test) (aka P319)."
For Karius collection and processing instructions, click here.
Reference Interval
No microorganisms detected
Synonyms
Metagenomic next-generation sequencing, deep sequencing
Stability (from collection to initiation)
CSF: Room temperature 6 hours or refrigerated 6 days, but prefer to freeze within 6 hours; stable frozen at -25C or -70C for 1 month.
Plasma: Room temperature up to 6 hours for whole blood; plasma frozen at -25C or -70C for 1 month.
Reported
1-2 weeks
Additional Information
Note, CSF is the only sample type accepted from outside UCSF
Test is interpreted by the Microbiology lab director and discussed with treating physicians as part of the Clinical Microbial Sequencing Board.
With approval from the Microbiology lab, plasma samples in select cases may be sent to Karius for testing. Order as "Microbiology - Test Not Listed (Special Sendout Test) (aka P319)."
For Karius collection and processing instructions, click here.
