Ordering Recommendations

May be useful for differential evaluation of polymyositis, dermatomyositis, necrotizing autoimmune myopathy, or overlap syndromes associated with connective tissue disease.

Available Stat

No

Performing Lab

ARUP

Performed

Sun-Sat

Methodology

Semi-Quantitative Enzyme-Linked Immunosorbent Assay (ELISA) / Qualitative Immunoprecipitation / Semi-Quantitative Multiplex Bead Assay / Qualitative Immunoblot / Semi-Quantitative Indirect Fluorescent Antibody (IFA) / Qualitative Particle-Based Multianalyte Technology (PMAT)

Reported

7-18 days

Additional Technical Information

Synonyms

  • U3RNP

Sample Type

Serum

Collect

Serum separator tube (SST), red top tube

Amount to Collect

6 mL blood

Preferred Volume

3 mL serum

Minimum Volume

1.5 mL serum

Stability (from collection to initiation)

Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 month

Storage/Transport Temperature

Refrigerated

Unacceptable Conditions

Hemolyzed, hyperlipemic, icteric, heat-treated, or contaminated specimens.

Test Code

MYOPAN

ARUP Test Code

3018867

Sendout

Yes

Performing Lab

ARUP

Specimen Preparation

Separate from cells ASAP or within 2 hours of collection. Transfer 3 to 4 mL to ARUP standard transport tube. (Min: 1.5 mL)

Additional Processing Instructions

Freeze aliquot. Transport aliquot frozen to CB. Order ARUP test code 2013961.

Preferred Volume

3 mL serum

Minimum Volume

1.5 mL serum

Unacceptable Conditions

Hemolyzed, hyperlipemic, icteric, heat-treated, or contaminated specimens.

Stability (from collection to initiation)

Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 month

Storage/Transport Temperature

Refrigerated

Reference Interval

Components
Reference Interval
HMGCR Antibody ScreenNegative
Zo (phenylalanyl-tRNA synthetase) AbNegative
Ks (asparaginyl-tRNA synthetase) AbNegative
Ha (tyrosyl-tRNA synthetase) AbNegative
TIF-1 gamma (155 kDa) AbNegative
NXP2 (Nuclear matrix protein-2) AbNegative
MDA5 (CADM-140) AbNegative
SAE1 (SUMO activating enzyme) AbNegative
Antinuclear Antibody (ANA), HEp-2, IgGLess than 1:80
Fibrillarin (U3 RNP) Ab, IgGNegative
SSA-60 (Ro60) (ENA) Antibody, IgG40 AU/mL or less
OJ (isoleucyl-tRNA synthetase) AntibodyNegative
SRP (Signal Recognition Particle) AbNegative
Ku AntibodyNegative
EJ (glycyl-tRNA synthetase) AntibodyNegative
P155/140 AntibodyNegative
PL-12 (alanyl-tRNA synthetase) AntibodyNegative
PL-7 (threonyl-tRNA synthetase) AntibodyNegative
Mi-2 (nuclear helicase protein) AntibodyNegative
PM/Scl 100 Antibody, IgGNegative
Jo-1 (Histidyl-tRNA Synthetase) Ab, IgG40 AU/mL or less
SSA-52 (Ro52) (ENA) Antibody, IgG40 AU/mL or less
Smith/RNP (ENA) Ab, IgG19 Units or less

Additional Technical Information

Interpretive Data

Refer to report.

Component
Interpretation
SSA-52 (Ro52) (ENA) Antibody, IgG29 AU/mL or less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive
SSA-60 (Ro60) (ENA) Antibody, IgG29 AU/mL or less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive
Smith/RNP (ENA) Antibody, IgG19 Units or less: Negative
20-39 Units: Weak Positive
40-80 Units: Moderate Positive
81 Units or greater:  Strong Positive
Jo-1 Antibody, IgG29 AU/mL or less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive

CPT Codes

83516 x13; 84182 x3; 86039; 86235 x6; if reflexed, add 83516

LOINC

  • 33983-8
  • 31625-7
  • 33571-1
  • 81732-0
  • 18485-3
  • 33772-5
  • 33771-7
  • 8061-4
  • 45149-2
  • 18484-6
  • 33921-8
  • 45152-6
  • 53019-6
  • 49963-2
  • 48767-8
  • 21424-7
  • 49311-4
  • 82415-1
  • 82422-7
  • 82453-2

Available Stat

No

Ordering Recommendations

May be useful for differential evaluation of polymyositis, dermatomyositis, necrotizing autoimmune myopathy, or overlap syndromes associated with connective tissue disease.

Test Code

MYOPAN

ARUP Test Code

3018867

Performing Lab

ARUP

Sendout

Yes

Performed

Sun-Sat

Methodology

Semi-Quantitative Enzyme-Linked Immunosorbent Assay (ELISA) / Qualitative Immunoprecipitation / Semi-Quantitative Multiplex Bead Assay / Qualitative Immunoblot / Semi-Quantitative Indirect Fluorescent Antibody (IFA) / Qualitative Particle-Based Multianalyte Technology (PMAT)

Collect

Serum separator tube (SST), red top tube

Amount to Collect

6 mL blood

Sample Type

Serum

Preferred Volume

3 mL serum

Minimum Volume

1.5 mL serum

Unacceptable Conditions

Hemolyzed, hyperlipemic, icteric, heat-treated, or contaminated specimens.

Specimen Preparation

Separate from cells ASAP or within 2 hours of collection. Transfer 3 to 4 mL to ARUP standard transport tube. (Min: 1.5 mL)

Additional Processing Instructions

Freeze aliquot. Transport aliquot frozen to CB. Order ARUP test code 2013961.

Reference Interval

Components
Reference Interval
HMGCR Antibody ScreenNegative
Zo (phenylalanyl-tRNA synthetase) AbNegative
Ks (asparaginyl-tRNA synthetase) AbNegative
Ha (tyrosyl-tRNA synthetase) AbNegative
TIF-1 gamma (155 kDa) AbNegative
NXP2 (Nuclear matrix protein-2) AbNegative
MDA5 (CADM-140) AbNegative
SAE1 (SUMO activating enzyme) AbNegative
Antinuclear Antibody (ANA), HEp-2, IgGLess than 1:80
Fibrillarin (U3 RNP) Ab, IgGNegative
SSA-60 (Ro60) (ENA) Antibody, IgG40 AU/mL or less
OJ (isoleucyl-tRNA synthetase) AntibodyNegative
SRP (Signal Recognition Particle) AbNegative
Ku AntibodyNegative
EJ (glycyl-tRNA synthetase) AntibodyNegative
P155/140 AntibodyNegative
PL-12 (alanyl-tRNA synthetase) AntibodyNegative
PL-7 (threonyl-tRNA synthetase) AntibodyNegative
Mi-2 (nuclear helicase protein) AntibodyNegative
PM/Scl 100 Antibody, IgGNegative
Jo-1 (Histidyl-tRNA Synthetase) Ab, IgG40 AU/mL or less
SSA-52 (Ro52) (ENA) Antibody, IgG40 AU/mL or less
Smith/RNP (ENA) Ab, IgG19 Units or less

Interpretive Data

Refer to report.

Component
Interpretation
SSA-52 (Ro52) (ENA) Antibody, IgG29 AU/mL or less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive
SSA-60 (Ro60) (ENA) Antibody, IgG29 AU/mL or less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive
Smith/RNP (ENA) Antibody, IgG19 Units or less: Negative
20-39 Units: Weak Positive
40-80 Units: Moderate Positive
81 Units or greater:  Strong Positive
Jo-1 Antibody, IgG29 AU/mL or less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive

Synonyms

  • U3RNP

Storage/Transport Temperature

Refrigerated

Stability (from collection to initiation)

Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 month

Reported

7-18 days

Additional Technical Information

CPT Codes

83516 x13; 84182 x3; 86039; 86235 x6; if reflexed, add 83516

LOINC

  • 33983-8
  • 31625-7
  • 33571-1
  • 81732-0
  • 18485-3
  • 33772-5
  • 33771-7
  • 8061-4
  • 45149-2
  • 18484-6
  • 33921-8
  • 45152-6
  • 53019-6
  • 49963-2
  • 48767-8
  • 21424-7
  • 49311-4
  • 82415-1
  • 82422-7
  • 82453-2

Notes

Antibodies: Mi-2, PL-7, PL12, P155/140, EJ, Ku, OJ, PM/Scl, SRP, Smith/RNP, Ro52, Ro60, Jo-1, U3 Fib, SAE1, NXP2, MDA5, TIF1-gamma, ANA, Ha, Ks, Zo

If HMGCR antibody, IgG is positive, additional testing to follow.
Ordering

Ordering Recommendations

May be useful for differential evaluation of polymyositis, dermatomyositis, necrotizing autoimmune myopathy, or overlap syndromes associated with connective tissue disease.

Available Stat

No

Performing Lab

ARUP

Performed

Sun-Sat

Methodology

Semi-Quantitative Enzyme-Linked Immunosorbent Assay (ELISA) / Qualitative Immunoprecipitation / Semi-Quantitative Multiplex Bead Assay / Qualitative Immunoblot / Semi-Quantitative Indirect Fluorescent Antibody (IFA) / Qualitative Particle-Based Multianalyte Technology (PMAT)

Reported

7-18 days

Additional Technical Information

Synonyms

  • U3RNP
Collection

Sample Type

Serum

Collect

Serum separator tube (SST), red top tube

Amount to Collect

6 mL blood

Preferred Volume

3 mL serum

Minimum Volume

1.5 mL serum

Stability (from collection to initiation)

Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 month

Storage/Transport Temperature

Refrigerated

Unacceptable Conditions

Hemolyzed, hyperlipemic, icteric, heat-treated, or contaminated specimens.
Processing

Test Code

MYOPAN

ARUP Test Code

3018867

Sendout

Yes

Performing Lab

ARUP

Specimen Preparation

Separate from cells ASAP or within 2 hours of collection. Transfer 3 to 4 mL to ARUP standard transport tube. (Min: 1.5 mL)

Additional Processing Instructions

Freeze aliquot. Transport aliquot frozen to CB. Order ARUP test code 2013961.

Preferred Volume

3 mL serum

Minimum Volume

1.5 mL serum

Unacceptable Conditions

Hemolyzed, hyperlipemic, icteric, heat-treated, or contaminated specimens.

Stability (from collection to initiation)

Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 month

Storage/Transport Temperature

Refrigerated
Result Interpretation

Reference Interval

Components
Reference Interval
HMGCR Antibody ScreenNegative
Zo (phenylalanyl-tRNA synthetase) AbNegative
Ks (asparaginyl-tRNA synthetase) AbNegative
Ha (tyrosyl-tRNA synthetase) AbNegative
TIF-1 gamma (155 kDa) AbNegative
NXP2 (Nuclear matrix protein-2) AbNegative
MDA5 (CADM-140) AbNegative
SAE1 (SUMO activating enzyme) AbNegative
Antinuclear Antibody (ANA), HEp-2, IgGLess than 1:80
Fibrillarin (U3 RNP) Ab, IgGNegative
SSA-60 (Ro60) (ENA) Antibody, IgG40 AU/mL or less
OJ (isoleucyl-tRNA synthetase) AntibodyNegative
SRP (Signal Recognition Particle) AbNegative
Ku AntibodyNegative
EJ (glycyl-tRNA synthetase) AntibodyNegative
P155/140 AntibodyNegative
PL-12 (alanyl-tRNA synthetase) AntibodyNegative
PL-7 (threonyl-tRNA synthetase) AntibodyNegative
Mi-2 (nuclear helicase protein) AntibodyNegative
PM/Scl 100 Antibody, IgGNegative
Jo-1 (Histidyl-tRNA Synthetase) Ab, IgG40 AU/mL or less
SSA-52 (Ro52) (ENA) Antibody, IgG40 AU/mL or less
Smith/RNP (ENA) Ab, IgG19 Units or less

Additional Technical Information

Interpretive Data

Refer to report.

Component
Interpretation
SSA-52 (Ro52) (ENA) Antibody, IgG29 AU/mL or less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive
SSA-60 (Ro60) (ENA) Antibody, IgG29 AU/mL or less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive
Smith/RNP (ENA) Antibody, IgG19 Units or less: Negative
20-39 Units: Weak Positive
40-80 Units: Moderate Positive
81 Units or greater:  Strong Positive
Jo-1 Antibody, IgG29 AU/mL or less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive

Administrative

CPT Codes

83516 x13; 84182 x3; 86039; 86235 x6; if reflexed, add 83516

LOINC

  • 33983-8
  • 31625-7
  • 33571-1
  • 81732-0
  • 18485-3
  • 33772-5
  • 33771-7
  • 8061-4
  • 45149-2
  • 18484-6
  • 33921-8
  • 45152-6
  • 53019-6
  • 49963-2
  • 48767-8
  • 21424-7
  • 49311-4
  • 82415-1
  • 82422-7
  • 82453-2
Complete View

Available Stat

No

Ordering Recommendations

May be useful for differential evaluation of polymyositis, dermatomyositis, necrotizing autoimmune myopathy, or overlap syndromes associated with connective tissue disease.

Test Code

MYOPAN

ARUP Test Code

3018867

Performing Lab

ARUP

Sendout

Yes

Performed

Sun-Sat

Methodology

Semi-Quantitative Enzyme-Linked Immunosorbent Assay (ELISA) / Qualitative Immunoprecipitation / Semi-Quantitative Multiplex Bead Assay / Qualitative Immunoblot / Semi-Quantitative Indirect Fluorescent Antibody (IFA) / Qualitative Particle-Based Multianalyte Technology (PMAT)

Collect

Serum separator tube (SST), red top tube

Amount to Collect

6 mL blood

Sample Type

Serum

Preferred Volume

3 mL serum

Minimum Volume

1.5 mL serum

Unacceptable Conditions

Hemolyzed, hyperlipemic, icteric, heat-treated, or contaminated specimens.

Specimen Preparation

Separate from cells ASAP or within 2 hours of collection. Transfer 3 to 4 mL to ARUP standard transport tube. (Min: 1.5 mL)

Additional Processing Instructions

Freeze aliquot. Transport aliquot frozen to CB. Order ARUP test code 2013961.

Reference Interval

Components
Reference Interval
HMGCR Antibody ScreenNegative
Zo (phenylalanyl-tRNA synthetase) AbNegative
Ks (asparaginyl-tRNA synthetase) AbNegative
Ha (tyrosyl-tRNA synthetase) AbNegative
TIF-1 gamma (155 kDa) AbNegative
NXP2 (Nuclear matrix protein-2) AbNegative
MDA5 (CADM-140) AbNegative
SAE1 (SUMO activating enzyme) AbNegative
Antinuclear Antibody (ANA), HEp-2, IgGLess than 1:80
Fibrillarin (U3 RNP) Ab, IgGNegative
SSA-60 (Ro60) (ENA) Antibody, IgG40 AU/mL or less
OJ (isoleucyl-tRNA synthetase) AntibodyNegative
SRP (Signal Recognition Particle) AbNegative
Ku AntibodyNegative
EJ (glycyl-tRNA synthetase) AntibodyNegative
P155/140 AntibodyNegative
PL-12 (alanyl-tRNA synthetase) AntibodyNegative
PL-7 (threonyl-tRNA synthetase) AntibodyNegative
Mi-2 (nuclear helicase protein) AntibodyNegative
PM/Scl 100 Antibody, IgGNegative
Jo-1 (Histidyl-tRNA Synthetase) Ab, IgG40 AU/mL or less
SSA-52 (Ro52) (ENA) Antibody, IgG40 AU/mL or less
Smith/RNP (ENA) Ab, IgG19 Units or less

Interpretive Data

Refer to report.

Component
Interpretation
SSA-52 (Ro52) (ENA) Antibody, IgG29 AU/mL or less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive
SSA-60 (Ro60) (ENA) Antibody, IgG29 AU/mL or less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive
Smith/RNP (ENA) Antibody, IgG19 Units or less: Negative
20-39 Units: Weak Positive
40-80 Units: Moderate Positive
81 Units or greater:  Strong Positive
Jo-1 Antibody, IgG29 AU/mL or less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive

Synonyms

  • U3RNP

Storage/Transport Temperature

Refrigerated

Stability (from collection to initiation)

Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 month

Reported

7-18 days

Additional Technical Information

CPT Codes

83516 x13; 84182 x3; 86039; 86235 x6; if reflexed, add 83516

LOINC

  • 33983-8
  • 31625-7
  • 33571-1
  • 81732-0
  • 18485-3
  • 33772-5
  • 33771-7
  • 8061-4
  • 45149-2
  • 18484-6
  • 33921-8
  • 45152-6
  • 53019-6
  • 49963-2
  • 48767-8
  • 21424-7
  • 49311-4
  • 82415-1
  • 82422-7
  • 82453-2

Notes

Antibodies: Mi-2, PL-7, PL12, P155/140, EJ, Ku, OJ, PM/Scl, SRP, Smith/RNP, Ro52, Ro60, Jo-1, U3 Fib, SAE1, NXP2, MDA5, TIF1-gamma, ANA, Ha, Ks, Zo

If HMGCR antibody, IgG is positive, additional testing to follow.

ARUP Consult®

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