EPIC Code

LAB3249
HSVPCR

Performing Lab

UCHealth University of Colorado Hospital Clinical Laboratory - Molecular Diagnostics

Collect

Specimen Source 
  • Swabs from lesion/vesicle fluid/rash/eye/skin
  • Eye swab
  • Bronchoalveolar lavage (BAL) or bronchial wash
  • Tracheal aspirate
  • CSF
  • Eye fluid

Minimum volume: >0.2 mL

Specimen Preparation

Lesion/vesicle fluid: Use a flocked swab to gently collect fluid from an unroofed lesion and place swab in M6/VTM/UTM/sterile PBS.
Other swabbed sources: Flocked swab placed in M6/VTM/UTM/sterile PBS.
BAL, tracheal aspirate, CSF, eye fluid: Transfer 1 mL (Min: >0.2 mL) of fluid to a sterile container.
Tissue or amniotic fluid: ARUP

Pediatric Collection

Infant multi-site testing: Use a single flocked swab to sequentially swab sites indicated on the order, working from "top to bottom" (e.g., eye, throat, then rectal). Place swab in M6, VTM, UTM, or sterile PBS. Do not use a wooden-shafted swab.

This is only acceptable for infants. For adults, pooled sources are not accepted. Testing multiple sites on adults should be submitted with one order and specimen per site.

Unacceptable Conditions

  • Sources other than those listed cannot be performed in-house.
  • Non-swabbed sources (e.g., BAL) submitted in preservative or media.
  • ESwabs.
  • Wooden-shafted, calcium alginate, or cotton swabs.
  • Sample not properly identified, incorrect container, insufficient volume, or past stability.
  • Lab Note: Samples processed in lab using non-sterile technique—including samples previously tested with a “common needle” on an instrument (e.g., Sysmex)—are unacceptable.

Storage/Transport Temperature

Internal: Ambient
Offsite: Refrigerated

Performed

Day shift: Monday–Friday

Stability (from collection to initiation)

Room Temp: 12 hours
Refrigerated: 3 days
Frozen: ≥31 days

Remarks

Specimen source must be identified on order or requisition.

Performed

Day shift: Monday–Friday

Methodology

Qualitative Real-time PCR

Reported

1–3 days

Synonyms

  • HSVPCR
  • HSV PCR
  • Herpes PCR
  • Herpes Simplex Virus PCR
  • 2010095 Herpes Simplex Virus (HSV-1/HSV-2) Subtype by PCR (HSVTYPEPCR/HSV TYPE PCR)
  • Herpes Simplex PCR

Ordering Recommendations

For HSV PCR whole blood testing see LAB3233.

Order may route to in-house or reference lab testing based upon "Specimen Type" and "Specimen Source" selected during ordering.

EPIC Code

LAB3249
HSVPCR

Reference Interval

Note: Separate qualitative results will usually be given for both HSV type 1 and HSV type 2. Occasionally some samples do not exhibit clear typing and these samples will be resulted as "Detected - unable to subtype."

HSV Type 1Not Detected
HSV Type 2Not Detected
Result comment:
Negative, no HSV detected.

Interpretive Data

Methodology: 
The Herpes Simplex Virus (HSV) 1/2 Qual by PCR is a qualitative nucleic acid amplification test for the detection and subtyping of HSV type 1 and type 2. This test was developed and its analytical performance characteristics determined by UCHealth Molecular Diagnostics Laboratory. It has not been cleared or approved by the U.S. Food and Drug Administration. This test was performed in a CLIA-certified laboratory and is intended for clinical purposes.

CPT Codes

87529 x2
Collection

EPIC Code

LAB3249
HSVPCR

Performing Lab

UCHealth University of Colorado Hospital Clinical Laboratory - Molecular Diagnostics

Collect

Specimen Source 
  • Swabs from lesion/vesicle fluid/rash/eye/skin
  • Eye swab
  • Bronchoalveolar lavage (BAL) or bronchial wash
  • Tracheal aspirate
  • CSF
  • Eye fluid

Minimum volume: >0.2 mL

Specimen Preparation

Lesion/vesicle fluid: Use a flocked swab to gently collect fluid from an unroofed lesion and place swab in M6/VTM/UTM/sterile PBS.
Other swabbed sources: Flocked swab placed in M6/VTM/UTM/sterile PBS.
BAL, tracheal aspirate, CSF, eye fluid: Transfer 1 mL (Min: >0.2 mL) of fluid to a sterile container.
Tissue or amniotic fluid: ARUP

Pediatric Collection

Infant multi-site testing: Use a single flocked swab to sequentially swab sites indicated on the order, working from "top to bottom" (e.g., eye, throat, then rectal). Place swab in M6, VTM, UTM, or sterile PBS. Do not use a wooden-shafted swab.

This is only acceptable for infants. For adults, pooled sources are not accepted. Testing multiple sites on adults should be submitted with one order and specimen per site.

Unacceptable Conditions

  • Sources other than those listed cannot be performed in-house.
  • Non-swabbed sources (e.g., BAL) submitted in preservative or media.
  • ESwabs.
  • Wooden-shafted, calcium alginate, or cotton swabs.
  • Sample not properly identified, incorrect container, insufficient volume, or past stability.
  • Lab Note: Samples processed in lab using non-sterile technique—including samples previously tested with a “common needle” on an instrument (e.g., Sysmex)—are unacceptable.

Storage/Transport Temperature

Internal: Ambient
Offsite: Refrigerated

Performed

Day shift: Monday–Friday

Stability (from collection to initiation)

Room Temp: 12 hours
Refrigerated: 3 days
Frozen: ≥31 days

Remarks

Specimen source must be identified on order or requisition.
Ordering

Performed

Day shift: Monday–Friday

Methodology

Qualitative Real-time PCR

Reported

1–3 days

Synonyms

  • HSVPCR
  • HSV PCR
  • Herpes PCR
  • Herpes Simplex Virus PCR
  • 2010095 Herpes Simplex Virus (HSV-1/HSV-2) Subtype by PCR (HSVTYPEPCR/HSV TYPE PCR)
  • Herpes Simplex PCR

Ordering Recommendations

For HSV PCR whole blood testing see LAB3233.

Order may route to in-house or reference lab testing based upon "Specimen Type" and "Specimen Source" selected during ordering.

EPIC Code

LAB3249
HSVPCR

Result Interpretation

Reference Interval

Note: Separate qualitative results will usually be given for both HSV type 1 and HSV type 2. Occasionally some samples do not exhibit clear typing and these samples will be resulted as "Detected - unable to subtype."

HSV Type 1Not Detected
HSV Type 2Not Detected
Result comment:
Negative, no HSV detected.

Interpretive Data

Methodology: 
The Herpes Simplex Virus (HSV) 1/2 Qual by PCR is a qualitative nucleic acid amplification test for the detection and subtyping of HSV type 1 and type 2. This test was developed and its analytical performance characteristics determined by UCHealth Molecular Diagnostics Laboratory. It has not been cleared or approved by the U.S. Food and Drug Administration. This test was performed in a CLIA-certified laboratory and is intended for clinical purposes.
Administrative

CPT Codes

87529 x2

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