EPIC Code

LAB3249
HSVPCR

Performing Lab

UCHealth University of Colorado Hospital Clinical Laboratory - Molecular Diagnostics

Collect

Effective Jan 16th, 2023, this test can only be performed in-house on the following sources:

  • Swabs from lesion/vesicle fluid/rash/eye/skin
  • Bronchoalveolar lavage (BAL) or bronchial wash
  • Tracheal aspirate
  • CSF
  • Eye fluid
  • Eye swab

Minimum volume: 0.2 mL

Specimen Preparation

Lesion/vesicle fluid: Use a flocked swab to gently collect fluid from an unroofed lesion and place swab in M6/VTM/UTM/sterile PBS.
Other swabbed sources: Flocked swab placed in M6/VTM/UTM/sterile PBS.
BAL, tracheal aspirate, CSF, eye fluid: Transfer 1 mL (Min: >0.2 mL) of fluid to a sterile container.
Tissue: ARUP

Pediatric Collection

Infant multi-site testing: E.g., Eye, throat, rectal. Use single flocked swab to swab according to sites on order (working “top to bottom”). Place swab in M6/VTM/UTM/sterile PBS. Do not use wooden-shafted swab.

This is only acceptable for infants. For adults, pooled sources are not accepted. Testing multiple sites on adults should be submitted with one order and specimen per site.

Unacceptable Conditions

  • Sources other than those listed cannot be performed in-house.
  • eSwabs.
  • Wooden-shafted or cotton swabs.
  • Sample not properly identified, incorrect container, insufficient volume, past stability, or leaking container.
  • Lab Note: Samples processed in lab using non-sterile technique—including samples previously tested with a “common needle” on an instrument—are unacceptable.

Storage/Transport Temperature

Internal: Ambient
Offsite: Refrigerated

Performed

Day shift: Monday–Friday

Stability (from collection to initiation)

Room Temp: 12 hours
Refrigerated: 3 days
Frozen: ≥31 days

Remarks

Specimen source must be identified on order or requisition.

Performed

Day shift: Monday–Friday

Methodology

Qualitative Real-time PCR

Reported

1–3 days

Synonyms

  • HSVPCR
  • HSV PCR

Ordering Recommendations

For HSV PCR whole blood testing see LAB3233.

EPIC Code

LAB3249
HSVPCR

Reference Interval

HSV Type 1 Not detected
HSV Type 2 Not detected

Note: Separate qualitative results will usually be given for both HSV type 1 and HSV type 2. Occasionally some samples do not exhibit clear typing and these samples will be resulted as "positive - unable to type."

Interpretive Data

Interpretation:
Not detected = Negative, no virus detected.
Detected = Positive, virus detected.

Methodology:
This test employs real-time PCR amplification of a Herpes simplex virus-specific conserved genetic target. A positive result should be coupled with clinical indicators for diagnosis.  A "Not detected" result for this assay does not exclude Herpes simplex virus involvement in a disease process. This test was developed and its performance characteristics determined by the UCH Clinical Laboratory.  It has not been cleared or approved by the U.S. Food and Drug Administration.  The FDA has determined that such clearance or approval is not necessary.  This test is used for clinical purposes.  It should not be regarded as investigational or for research. This laboratory is certified under the Clinical Laboratory Improvement Amendment of 1988 ("CLIA") as qualified to perform high complexity clinical testing.

CPT Codes

87529 (2)

Collection

EPIC Code

LAB3249
HSVPCR

Performing Lab

UCHealth University of Colorado Hospital Clinical Laboratory - Molecular Diagnostics

Collect

Effective Jan 16th, 2023, this test can only be performed in-house on the following sources:

  • Swabs from lesion/vesicle fluid/rash/eye/skin
  • Bronchoalveolar lavage (BAL) or bronchial wash
  • Tracheal aspirate
  • CSF
  • Eye fluid
  • Eye swab

Minimum volume: 0.2 mL

Specimen Preparation

Lesion/vesicle fluid: Use a flocked swab to gently collect fluid from an unroofed lesion and place swab in M6/VTM/UTM/sterile PBS.
Other swabbed sources: Flocked swab placed in M6/VTM/UTM/sterile PBS.
BAL, tracheal aspirate, CSF, eye fluid: Transfer 1 mL (Min: >0.2 mL) of fluid to a sterile container.
Tissue: ARUP

Pediatric Collection

Infant multi-site testing: E.g., Eye, throat, rectal. Use single flocked swab to swab according to sites on order (working “top to bottom”). Place swab in M6/VTM/UTM/sterile PBS. Do not use wooden-shafted swab.

This is only acceptable for infants. For adults, pooled sources are not accepted. Testing multiple sites on adults should be submitted with one order and specimen per site.

Unacceptable Conditions

  • Sources other than those listed cannot be performed in-house.
  • eSwabs.
  • Wooden-shafted or cotton swabs.
  • Sample not properly identified, incorrect container, insufficient volume, past stability, or leaking container.
  • Lab Note: Samples processed in lab using non-sterile technique—including samples previously tested with a “common needle” on an instrument—are unacceptable.

Storage/Transport Temperature

Internal: Ambient
Offsite: Refrigerated

Performed

Day shift: Monday–Friday

Stability (from collection to initiation)

Room Temp: 12 hours
Refrigerated: 3 days
Frozen: ≥31 days

Remarks

Specimen source must be identified on order or requisition.
Ordering

Performed

Day shift: Monday–Friday

Methodology

Qualitative Real-time PCR

Reported

1–3 days

Synonyms

  • HSVPCR
  • HSV PCR

Ordering Recommendations

For HSV PCR whole blood testing see LAB3233.

EPIC Code

LAB3249
HSVPCR

Result Interpretation

Reference Interval

HSV Type 1 Not detected
HSV Type 2 Not detected

Note: Separate qualitative results will usually be given for both HSV type 1 and HSV type 2. Occasionally some samples do not exhibit clear typing and these samples will be resulted as "positive - unable to type."

Interpretive Data

Interpretation:
Not detected = Negative, no virus detected.
Detected = Positive, virus detected.

Methodology:
This test employs real-time PCR amplification of a Herpes simplex virus-specific conserved genetic target. A positive result should be coupled with clinical indicators for diagnosis.  A "Not detected" result for this assay does not exclude Herpes simplex virus involvement in a disease process. This test was developed and its performance characteristics determined by the UCH Clinical Laboratory.  It has not been cleared or approved by the U.S. Food and Drug Administration.  The FDA has determined that such clearance or approval is not necessary.  This test is used for clinical purposes.  It should not be regarded as investigational or for research. This laboratory is certified under the Clinical Laboratory Improvement Amendment of 1988 ("CLIA") as qualified to perform high complexity clinical testing.

Administrative

CPT Codes

87529 (2)

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