Blood or Bone Marrow: 2-3 mL in a purple top EDTA tube.
Minimum Collection Volume
2 mL of whole peripheral blood or bone marrow.
If a lesser volume of blood is sent, the laboratory will attempt to perform the test requested. The laboratory cannot assure either a result and/or the ability to perform repeat testing and/or additional testing if the minimum volumes are not met.
Acceptable Specimen Collect Alternatives
Dark Green Sodium Heparin tube is also acceptable for Blood and Bone Marrow collection.
Cells: Sterile 25cm2 culture flask with monolayer growth; fill with media and tighten cap when transporting to lab. -OR- Prenatal samples: 20-25 mL amniotic fluid or two T25 flasks. -OR- Tissue: Fresh tissue for cell culture sent in sterile media or saline (not frozen). DO NOT USE FORMALIN for tissue specimens, the tissue must be fresh and sterile in order to obtain cell growth.
Shipping/Handling Instructions (Lab Use Only)
Room Temperature. Do NOT spin. Burnet: Call GENETICS (6-4474) immediately upon arrival.
External Client Shipping and Handling
Store at room temperature / Use overnight shipping (protect from temperature extremes, no ice)
Stability (from collection to initiation)
SEND TO GENETICS to determine viability of specimen; Call the Genetics Lab at 513-636-4474 for pick up or for any questions or concerns about the specimen.
Notes
Please NOTE: THIS TEST IS NOT VALIDATED FOR ONCOLOGY ANALYSIS
Sample MUST be received by the lab within 4 days or it will be considered unacceptable. Label tubes with patient name and date of birth (DOB).
If the specimen was obtained more than 4 days prior to receipt in lab, it will be considered unacceptable and this testing cannot be performed.
Synonyms
OGM
Bionano
Structural variant analysis
Methodology
OGM technology labels ultra-high molecular weight DNA at a specific sequence motif (CTTAAG) occurring approximately 14-16 times per 100kb in the human genome. The long fluorescently labeled DNA molecules are linearized and imaged in nanochannel arrays in a high throughput, automated manner. High-resolution images of labeled motifs are converted into single molecule contigs and assembled into whole genome de novo maps with approximately 800 gigabytes of data. Structural variant calling is based on changes in the coverage pattern or spacing of the labels. Clinically relevant SVs that pass quality control thresholds with sufficient labeling motifs will be reported.
Suspicion for complex chromosomal events by prior testing
Unsolved diagnosis after other testing
CPT Codes
81354
Please call 1-866-450-4198 for pricing or with any billing questions.
LOINC Code(s)
94087-4
Collection
Collect
Blood or Bone Marrow: 2-3 mL in a purple top EDTA tube.
Minimum Collection Volume
2 mL of whole peripheral blood or bone marrow.
If a lesser volume of blood is sent, the laboratory will attempt to perform the test requested. The laboratory cannot assure either a result and/or the ability to perform repeat testing and/or additional testing if the minimum volumes are not met.
Acceptable Specimen Collect Alternatives
Dark Green Sodium Heparin tube is also acceptable for Blood and Bone Marrow collection.
Cells: Sterile 25cm2 culture flask with monolayer growth; fill with media and tighten cap when transporting to lab. -OR- Prenatal samples: 20-25 mL amniotic fluid or two T25 flasks. -OR- Tissue: Fresh tissue for cell culture sent in sterile media or saline (not frozen). DO NOT USE FORMALIN for tissue specimens, the tissue must be fresh and sterile in order to obtain cell growth.
Shipping/Handling Instructions (Lab Use Only)
Room Temperature. Do NOT spin. Burnet: Call GENETICS (6-4474) immediately upon arrival.
External Client Shipping and Handling
Store at room temperature / Use overnight shipping (protect from temperature extremes, no ice)
Stability (from collection to initiation)
SEND TO GENETICS to determine viability of specimen; Call the Genetics Lab at 513-636-4474 for pick up or for any questions or concerns about the specimen.
Notes
Please NOTE: THIS TEST IS NOT VALIDATED FOR ONCOLOGY ANALYSIS
Sample MUST be received by the lab within 4 days or it will be considered unacceptable. Label tubes with patient name and date of birth (DOB).
If the specimen was obtained more than 4 days prior to receipt in lab, it will be considered unacceptable and this testing cannot be performed.
Ordering
Synonyms
OGM
Bionano
Structural variant analysis
Methodology
OGM technology labels ultra-high molecular weight DNA at a specific sequence motif (CTTAAG) occurring approximately 14-16 times per 100kb in the human genome. The long fluorescently labeled DNA molecules are linearized and imaged in nanochannel arrays in a high throughput, automated manner. High-resolution images of labeled motifs are converted into single molecule contigs and assembled into whole genome de novo maps with approximately 800 gigabytes of data. Structural variant calling is based on changes in the coverage pattern or spacing of the labels. Clinically relevant SVs that pass quality control thresholds with sufficient labeling motifs will be reported.
