Blood or Bone Marrow: 2-3 mL in a purple top EDTA tube. Green top NaHep is also acceptable, but EDTA is preferred.
-OR- Cells: Sterile 25cm2 culture flask with monolayer growth; fill with media and tighten cap when transporting to lab.
-OR- Prenatal samples: 20-25 mL amniotic fluid or two T25 flasks.
-OR- Tissue: Fresh tissue for cell culture sent in sterile media or saline (not frozen).
Minimum Collection Volume
2 mL of whole peripheral blood or bone marrow.
If a lesser volume of blood is sent, the laboratory will attempt to perform the test requested. The laboratory cannot assure either a result and/or the ability to perform repeat testing and/or additional testing if the minimum volumes are not met.
Acceptable Specimen Collect Alternatives
Dark Green Sodium Heparin tube is also acceptable.
Patient Preparation
The lab can provide tubes of transport medium - Call (513) 636-4474.
DO NOT USE FORMALIN for tissue specimens, the tissue must be fresh and sterile in order to obtain cell growth.
Sample MUST be received by the lab within 4 days or it will be considered unacceptable.
Specimen Preparation
Label tubes with patient name and date of birth (DOB).
Storage/Transport Temperature
Store at room temp; Must be extracted 4 days from draw.
If the specimen was obtained more than 4 days prior to receipt in lab, it will be considered unacceptable and this testing cannot be performed.
Synonyms
OGM
Bionano
Structural variant analysis
Methodology
OGM technology labels ultra-high molecular weight DNA at a specific sequence motif (CTTAAG) occurring approximately 14-16 times per 100kb in the human genome. The long fluorescently labeled DNA molecules are linearized and imaged in nanochannel arrays in a high throughput, automated manner. High-resolution images of labeled motifs are converted into single molecule contigs and assembled into whole genome de novo maps with approximately 800 gigabytes of data. Structural variant calling is based on changes in the coverage pattern or spacing of the labels. Clinically relevant SVs that pass quality control thresholds with sufficient labeling motifs will be reported.
Suspicion for complex chromosomal events by prior testing
Unsolved diagnosis after other testing
CPT Codes
81479
Please call 1-866-450-4198 for pricing or with any billing questions.
LOINC Code(s)
94087-4
Collection
Collect
Blood or Bone Marrow: 2-3 mL in a purple top EDTA tube. Green top NaHep is also acceptable, but EDTA is preferred.
-OR- Cells: Sterile 25cm2 culture flask with monolayer growth; fill with media and tighten cap when transporting to lab.
-OR- Prenatal samples: 20-25 mL amniotic fluid or two T25 flasks.
-OR- Tissue: Fresh tissue for cell culture sent in sterile media or saline (not frozen).
Minimum Collection Volume
2 mL of whole peripheral blood or bone marrow.
If a lesser volume of blood is sent, the laboratory will attempt to perform the test requested. The laboratory cannot assure either a result and/or the ability to perform repeat testing and/or additional testing if the minimum volumes are not met.
Acceptable Specimen Collect Alternatives
Dark Green Sodium Heparin tube is also acceptable.
Patient Preparation
The lab can provide tubes of transport medium - Call (513) 636-4474.
DO NOT USE FORMALIN for tissue specimens, the tissue must be fresh and sterile in order to obtain cell growth.
Sample MUST be received by the lab within 4 days or it will be considered unacceptable.
Specimen Preparation
Label tubes with patient name and date of birth (DOB).
Storage/Transport Temperature
Store at room temp; Must be extracted 4 days from draw.
If the specimen was obtained more than 4 days prior to receipt in lab, it will be considered unacceptable and this testing cannot be performed.
Ordering
Synonyms
OGM
Bionano
Structural variant analysis
Methodology
OGM technology labels ultra-high molecular weight DNA at a specific sequence motif (CTTAAG) occurring approximately 14-16 times per 100kb in the human genome. The long fluorescently labeled DNA molecules are linearized and imaged in nanochannel arrays in a high throughput, automated manner. High-resolution images of labeled motifs are converted into single molecule contigs and assembled into whole genome de novo maps with approximately 800 gigabytes of data. Structural variant calling is based on changes in the coverage pattern or spacing of the labels. Clinically relevant SVs that pass quality control thresholds with sufficient labeling motifs will be reported.
