3-6 mL of whole peripheral blood in Lavender top EDTA tube(s).
Collection personnel MUST initial specimen container to confirm sample identity.
If DNA is sent, it MUST have been extracted from a lavender EDTA tube in a CLIA certified laboratory. Use TE buffer in 1.5 mL Eppendorf tube.
Minimum Collection Volume
3mL peripheral blood
1.5mcg DNA
If a lesser volume of sample listed above is sent, the laboratory will attempt to perform the test requested. The laboratory cannot assure either a result and/or the ability to perform repeat testing and/or additional testing if the minimum volumes are not met.
Specimen Preparation
Label tubes with patient name and date of birth (DOB).
Storage/Transport Temperature
Store at room temperature / Use overnight shipping (protect from temperature extremes, no ice) or call the lab for local courier service (contact laboratory prior to drawing specimen to ensure courier area coverage).
HOURS of OPERATION: 6 AM - 12 AM (Monday through Friday, with more limited hours on the weekend)
Ordering Recommendations
See Episignature Analysis by Methylation Array Appendix Table under Related Information.
Methodology
Genomic DNA was isolated from peripheral blood and treated for bisulfite conversion using the EZ DNA methylation kit (Zymo Research). Genome-wide DNA methylation measurements were processed using the Infinium Assay with the Methylation EPIC v2.0 BeadChip platform (Illumina). This chip contains approximately 935K genome-wide CpG markers. The disease-associated episignature profile was assessed using the clinically validated EpiSignTM classifier (version 5) and the EpiSignTM Knowledge Database at the London Health Sciences Center (London, ON, Canada; https://episign.lhsc.on.ca/). The EpiSignTM classifier is designed to select probes that are both highly sensitive and specific for a cohort of interest and uses machine learning-based algorithms to compare patient’s blood DNA methylation to reference samples (PMID: 28807811; PMID: 35047860; PMID: 33547396; PMID: 38251460).
Please call 1-866-450-4198 for pricing or with any billing questions.
Minimum Testing Volume
3mL peripheral blood
1.5mcg DNA
If a lesser volume of sample listed above is sent, the laboratory will attempt to perform the test requested. The laboratory cannot assure either a result and/or the ability to perform repeat testing and/or additional testing if the minimum volumes are not met.
Collection
Collect
3-6 mL of whole peripheral blood in Lavender top EDTA tube(s).
Collection personnel MUST initial specimen container to confirm sample identity.
If DNA is sent, it MUST have been extracted from a lavender EDTA tube in a CLIA certified laboratory. Use TE buffer in 1.5 mL Eppendorf tube.
Minimum Collection Volume
3mL peripheral blood
1.5mcg DNA
If a lesser volume of sample listed above is sent, the laboratory will attempt to perform the test requested. The laboratory cannot assure either a result and/or the ability to perform repeat testing and/or additional testing if the minimum volumes are not met.
Specimen Preparation
Label tubes with patient name and date of birth (DOB).
Storage/Transport Temperature
Store at room temperature / Use overnight shipping (protect from temperature extremes, no ice) or call the lab for local courier service (contact laboratory prior to drawing specimen to ensure courier area coverage).
HOURS of OPERATION: 6 AM - 12 AM (Monday through Friday, with more limited hours on the weekend)
Ordering
Ordering Recommendations
See Episignature Analysis by Methylation Array Appendix Table under Related Information.
Methodology
Genomic DNA was isolated from peripheral blood and treated for bisulfite conversion using the EZ DNA methylation kit (Zymo Research). Genome-wide DNA methylation measurements were processed using the Infinium Assay with the Methylation EPIC v2.0 BeadChip platform (Illumina). This chip contains approximately 935K genome-wide CpG markers. The disease-associated episignature profile was assessed using the clinically validated EpiSignTM classifier (version 5) and the EpiSignTM Knowledge Database at the London Health Sciences Center (London, ON, Canada; https://episign.lhsc.on.ca/). The EpiSignTM classifier is designed to select probes that are both highly sensitive and specific for a cohort of interest and uses machine learning-based algorithms to compare patient’s blood DNA methylation to reference samples (PMID: 28807811; PMID: 35047860; PMID: 33547396; PMID: 38251460).
Please call 1-866-450-4198 for pricing or with any billing questions.
Minimum Testing Volume
3mL peripheral blood
1.5mcg DNA
If a lesser volume of sample listed above is sent, the laboratory will attempt to perform the test requested. The laboratory cannot assure either a result and/or the ability to perform repeat testing and/or additional testing if the minimum volumes are not met.
