If a lesser volume of sample listed above is sent, the laboratory will attempt to perform the test requested. The laboratory cannot assure either a result and/or the ability to perform repeat testing and/or additional testing if the minimum volumes are not met.
Shipping/Handling Instructions (Lab Use Only)
Room Temperature. Do NOT spin. Burnet: Call GENETICS (6-4474) immediately upon arrival.
External Client Shipping and Handling
Store at room temperature / Use overnight shipping (protect from temperature extremes, no ice) or call the lab for local courier service (contact laboratory prior to drawing specimen to ensure courier area coverage). Do NOT spin tubes.
Stability (from collection to initiation)
SEND TO GENETICS to determine viability of specimen; Call the Genetics Lab at 513-636-4474 for pick up or for any questions or concerns about the specimen.
Notes
If DNA is sent, it MUST have been extracted from a lavender EDTA tube in a CLIA certified laboratory. Use TE buffer in 1.5 mL Eppendorf tube.
Collection personnel MUST initial specimen container to confirm sample identity. Label tubes with patient name and date of birth (DOB).
See Episignature Analysis by Methylation Array Appendix Table under Related Information.
Synonyms
EPISIGN
EPISIGNATURE
METHYLATION ANALYSIS
IMPRINTING DISORDER TESTING BY METHYLATION ARRAY
METHYLATION ARRAY
Methodology
Genomic DNA was isolated from peripheral blood and treated for bisulfite conversion using the EZ DNA methylation kit (Zymo Research). Genome-wide DNA methylation measurements were processed using the Infinium Assay with the Methylation EPIC v2.0 BeadChip platform (Illumina). This chip contains approximately 935K genome-wide CpG markers. The disease-associated episignature profile was assessed using the clinically validated EpiSignTM classifier (version 5) and the EpiSignTM Knowledge Database at the London Health Sciences Center (London, ON, Canada; https://episign.lhsc.on.ca/). The EpiSignTM classifier is designed to select probes that are both highly sensitive and specific for a cohort of interest and uses machine learning-based algorithms to compare patient’s blood DNA methylation to reference samples (PMID: 28807811; PMID: 35047860; PMID: 33547396; PMID: 38251460).
Please call 1-866-450-4198 for pricing or with any billing questions.
Minimum Testing Volume
3mL peripheral blood
1.5mcg DNA
If a lesser volume of sample listed above is sent, the laboratory will attempt to perform the test requested. The laboratory cannot assure either a result and/or the ability to perform repeat testing and/or additional testing if the minimum volumes are not met.
Collection
Collect
3-6 mL Blood in 3mL Lav EDTA.
Minimum Collection Volume
3mL peripheral blood
1.5mcg DNA
If a lesser volume of sample listed above is sent, the laboratory will attempt to perform the test requested. The laboratory cannot assure either a result and/or the ability to perform repeat testing and/or additional testing if the minimum volumes are not met.
Shipping/Handling Instructions (Lab Use Only)
Room Temperature. Do NOT spin. Burnet: Call GENETICS (6-4474) immediately upon arrival.
External Client Shipping and Handling
Store at room temperature / Use overnight shipping (protect from temperature extremes, no ice) or call the lab for local courier service (contact laboratory prior to drawing specimen to ensure courier area coverage). Do NOT spin tubes.
Stability (from collection to initiation)
SEND TO GENETICS to determine viability of specimen; Call the Genetics Lab at 513-636-4474 for pick up or for any questions or concerns about the specimen.
Notes
If DNA is sent, it MUST have been extracted from a lavender EDTA tube in a CLIA certified laboratory. Use TE buffer in 1.5 mL Eppendorf tube.
Collection personnel MUST initial specimen container to confirm sample identity. Label tubes with patient name and date of birth (DOB).
See Episignature Analysis by Methylation Array Appendix Table under Related Information.
Synonyms
EPISIGN
EPISIGNATURE
METHYLATION ANALYSIS
IMPRINTING DISORDER TESTING BY METHYLATION ARRAY
METHYLATION ARRAY
Methodology
Genomic DNA was isolated from peripheral blood and treated for bisulfite conversion using the EZ DNA methylation kit (Zymo Research). Genome-wide DNA methylation measurements were processed using the Infinium Assay with the Methylation EPIC v2.0 BeadChip platform (Illumina). This chip contains approximately 935K genome-wide CpG markers. The disease-associated episignature profile was assessed using the clinically validated EpiSignTM classifier (version 5) and the EpiSignTM Knowledge Database at the London Health Sciences Center (London, ON, Canada; https://episign.lhsc.on.ca/). The EpiSignTM classifier is designed to select probes that are both highly sensitive and specific for a cohort of interest and uses machine learning-based algorithms to compare patient’s blood DNA methylation to reference samples (PMID: 28807811; PMID: 35047860; PMID: 33547396; PMID: 38251460).
Please call 1-866-450-4198 for pricing or with any billing questions.
Minimum Testing Volume
3mL peripheral blood
1.5mcg DNA
If a lesser volume of sample listed above is sent, the laboratory will attempt to perform the test requested. The laboratory cannot assure either a result and/or the ability to perform repeat testing and/or additional testing if the minimum volumes are not met.
