Collect

1-3 mL of Bone Marrow or Oncology Blood in a Dark Green top sodium (Na) heparin tube.
    - The preferred choice for specimen collection is listed above, however, if that tube type/container is not available please call the lab for advice on alternative specimen collection choices (513-636-4474).  If the specimen is already collected in a different tube type, send the sample to the lab and we will make every effort to process and result the test.

* PET (paraffin embedded tissue) specimen type (slides or block) can be sent for these FISH Probes

Specify what TYPE of FISH is required on the requisition. If the FISH probe test that you require is not listed on the test requisition, please hand-write the name of the test you want to order in the space provided.

Collection personnel MUST initial specimen container to confirm sample identity.

Minimum Collection Volume

1-3 mL of Bone Marrow or Oncology Blood in a Dark Green top sodium (Na) heparin tube.

If a lesser volume of bone marrow or blood is sent, the laboratory will attempt to perform the test requested.  The laboratory cannot assure either a result and/or the ability to perform repeat testing and/or additional testing if the minimum volumes are not met.

Acceptable Specimen Collect Alternatives

Acceptable:

Bone Marrow/Oncology Blood in sterile culture medium.

Bone Marrow/Oncology Blood in a sterile syringe with NaHep (sodium heparin) added.

For any tube other than those listed in the UNACCEPTABLE CONDITION field (see below) we will attempt to set up and try to obtain FISH results.

Patient Preparation

Please go to "ORDERING" tab to see a complete list of the stand-alone FISH Probes we offer for oncology testing.  Please use our Test Requisition (see link at bottom of next tab) and fill in the FISH probe you require.  If you do not see a FISH probe that you need in the listing, please call our lab to see if we can order it for your testing.

Specimen Preparation

Label tubes with patient name and date of birth (DOB).

Storage/Transport Temperature

Store at room temperature  / Use overnight shipping (protect from temperature extremes, no ice) or call the lab for local courier service (contact laboratory prior to drawing specimen to ensure courier area coverage).

Performing Lab

Cytogenetics Laboratory (513) 636-4474  /  FAX: (513) 636-4373

Performed

6 AM - 12 AM (Monday through Friday, with more limited hours on the weekend)

Unacceptable Conditions

DO NOT SEND if sample has been drawn in a:

CLOT TUBE

NaCitrate Tube

Serum Separation Tube that has been centrifuged (an unspun tube is acceptable)

Synonyms

  • 4398422 - FISH : ATM (11q22.3) / p53 (17p13.1)
  • 4623068 - FISH : CDKN2A [p16] (9p21) / CEP 9
  • 5096865 - FISH : 13q14.3 / 13q34
  • 4395187 - FISH : MALT1 (18q21)
  • 4750590 - FISH : T(8 14) MYC/IGH
  • 4750280 - FISH : MONO 7 / DEL 7q (CEP 7 / D7S522)
  • 4388818 - FISH : MALT T(14 18) [IGH / MALT1] [NHL]
  • 4750250 - FISH : T(9 22) [BCR / ABL] - PHILA CHROM
  • 4750260 - FISH : T(15 17) [PML / RARa]
  • 4750700 - FISH : 20q12
  • 4750690 - FISH : MONO 5 / DELETION 5q (EGR1)
  • 4395134 - FISH : ETV6 (12p13)
  • 4750500 - FISH : BCL6 (3q27)
  • 4395176 - FISH : RARa (17q21)
  • 4388766 - FISH : T(11 18) [API2 / MALT1]
  • 4395160 - FISH : IGH (14q32.3)
  • 4750540 - FISH : KMT2A [MLL] (11q23)
  • 4750230 - FISH : XY (BMT ONC)
  • 4750530 - FISH : T(12 21) ETV6 [TEL] / RUNX1[AML1]
  • 4750520 - FISH : T(14 18) [IGH/BCL2] - FOLL. LYMPH
  • 4388795 - FISH : T(14 16) [IGH / MAF]
  • 4398428 - FISH : CEP 12 / 13q14.3 / 13q34
  • 4398415 - FISH : PTEN (10q23)
  • 4750550 - FISH : MYC (8q24)
  • 4750270 - FISH : CEP 8
  • 4398457 - FISH : TP53 (17p13.1)
  • 4395202 - FISH : SYT (18q11.2) - SYNOVIAL SARCOMA
  • 4398327 - FISH : MYCN (2p24.1)
  • 4398464 - FISH : 19p13 / 19q13
  • 4395078 - FISH : ALK (2p23)
  • 4388678 - FISH : T(4 14) [IGH / FGFR3]
  • 4398445 - FISH : D13S319 (13q14.3)
  • 4398338 - FISH : 4q12 (CHIC2, FIP1L1, KIT, LNX, PDGFRA, SCFD2)
  • 5358195 - FISH : EVI1 (3q26.2)
  • 4750680 - FISH : 1p36 /1q25 (ONCOLOGY)
  • 4398338 - FISH : 4q12 - HYPEREOSINOPHILIA
  • 4398451 - FISH : 13q34
  • 4395149 - FISH : FOXO1 [FKHR] (13q14) - RHABDOMYOSARCOMA
  • 4398409 - FISH : 9q34 (ASS)
  • 7291943 - FISH : RCC TFE3 (Xp11.23)
  • 7299733 - FISH : RCC ASPSCR1/PRCC/TFE3 (tricolor DF)
  • 4398397 - FISH : MYB (6q23)
  • 4395217 - FISH : EWSR1 (22q12) - EWING SARCOMA
  • 4395169 - FISH : INVERSION 16 / T(16 16) [CBFB]
  • 7409183 - FISH : USP6 (17p13)
  • 7409180 - FISH : BCL2 (18q21.3)
  • 7291954 - FISH : 1q21 (CKS1B)
  • 7554881 - FISH : BRAF (7q34)
  • 4750480 - FISH : t(11 14) [IGH / CCND1 XT]
  • 4750710 - FISH : t(8 21) RUNX1T1 (ETO)/RUNX1 (AML1)
  • 4399168 - FISH : Aneuploidy 21 (for Oncology)
  • 7530897 - FISH : FUS (16p11)
  • 4750290 - FISH : Miscellaneous FISH (for Oncology)
  • 4398434 - FISH : RB-1 (13q14) (for Oncology)
  • FISH Probe : Somatic (252)

Tests Included

If the FISH probe test that you require is not listed on the test requisition, please hand-write the name of the test you want to order in the space provided.
t(X;1) / t(X;17)
Tricolor Dual Fusion for ASPSCR1/PRCC/TFE3 (RCC)
t(4;14)
LSI IgH (14q32.3)/FGFR3 (4p16) (Dual Fusion)*
t(8;14)
LSI IgH (14q32.3)/ MYC (8q24)/CEP 8 (Dual Fusion)*
t(8;21)
LSI RUNX1T1 (ETO)(8q21.3)/LSI RUNX1 (AML1) (21q22) (Dual Fusion)*
t(9;22)
LSI BCR (22q11.2)/ABL1 (9q34) (Dual Fusion)*
t(11;14)
LSI IgH (14q32.3)/CCND1 (11q13) XT (Dual Fusion)*
t(11;18)
LSI BIRC3 (11q21)/MALT1 (18q21) (Dual Fusion)*
t(12;21)
LSI ETV6 (TEL) (12p13)/LSI RUNX1 (AML1) (21q22) (ES Fusion)*
t(14;16)
LSI IgH (14q32.3)/MAF (16q32) (Dual Fusion)*
t(14;18)
LSI IgH (14q32.3)/LSI BCL2 (18q21) (Dual Fusion)*
t(14;18)
LSI IgH (14q32.3)/LSI MALT1 (18q21) (Dual Fusion)*
t(15;17)
LSI PML (15q22)/RARA (17q21) (Dual Fusion* 



X Chrom  TFE3 (Xp11.23)  - Breakapart (for RCC)
Chrom 1
LSI 1p36 (EGFL3, TP73)/LSI 1q25 (ABL2)
1q21/1p32.3 [CKS1B/CDKN2C] (p18) Amplification/Deletion Probe
Chrom 2
LSI MYCN (2p24.1)/LAF (2q11) (Amplification)
LSI ALK (2p23) (Break Apart)*
Chrom 3
LSI EVI1 (3q26) (TC BA Probe)*
LSI BCL6 (3q27) (Break Apart)*
Chrom 4
LSI 4q12 (SCFD2, FIP1L1/LNX,PDGFRA/KIT) (TC BA Probe)*
Chrom 5
LSI EGR1 (5q31)/D5S23,D5S721 (5p15.2)
PDGFRB (5q32) (Break Apart)*
Chrom 6
LSI MYB (6q23)/CEP 6
Chrom 7
LSI D7S522 (7q31)/CEP 7
EGFR (7p11.2)/CEP 7 (Amplification)
TRG (7p14) (Break Apart)*
TRB (7q34) (Break Apart)*
BRAF (7q34) (Break Apart)*
Chrom 8
LSI MYC (8q24) (Break Apart)*
FGFR1 (8p11) (TC Break Apart/Amplification)*
Chrom 9
LSI CDKN2A [p16] (9p21)/CEP 9
LSI ASS (9q34)
Chrom 10
LSI PTEN (10q32)/CEP 10
Chrom 11
LSI KMT2A [MLL] (11q23) (Break Apart)*
Chrom 11/17
LSI ATM (11q22.3)/LSI p53 (17p13.1)
Chrom 12
LSI CHOP (DDIT3) (12q13) (Break Apart)*
LSI ETV6 (TEL) (12p13) (Break Apart)*
LSI MDM2 (12q15)/CEP 12
Chrom 12/13
LSI D13S319 (13q14.3)/LAMP1 (13q34)/CEP 12
Chrom 13
LSI 13 (RB1) (13q14)
LSI D13S319 (13q14.3)
LSI FOXO1 [FKHR] (13q14) (Break Apart)*
LSI LAMP1 (13q34)
Chrom 14
LSI IgH (14q32.3) (Break Apart)*
LSI TCR alpha/delta (14q11.2) (Break Apart)*
TRAD (14q11.2) (Break Apart)*
Chrom 16
LSI FUS (16p11) (Break Apart)*
LSI CBFB (16q22) (Break Apart)*
CBFB (16q22)/MYH11 (16p13) (Dual Fusion Translocation Probe)*
Chrom 17
LSI TP53 [p53] (17p13.1)
LSI RARA (17q21) (Break Apart)*

Chrom 18
BCL2 (18q21.3) (Break Apart)
LSI MALT1 (18q21) (Break Apart)*
LSI SYT (18q11.2) (Break Apart)*
Chrom 19
LSI 19q13/19p13
LSI E2A (19p13) (Break Apart)*
Chrom 20
LSI D20S108 (20q12)
Chrom 21
LSI 21 (21q22.13-q22.2)
Chrom 22
LSI 22 (BCR) (22q11.2)
LSI EWSR1 (22q12) (Break Apart)*

Sex Mismatched BMT CEP X (SO) / Y (SG)

Methodology

Fluorescence In Situ Hybridization

If multiple single FISH or FISH Panel tests are ordered at the same time, only ONE culture charge is added for the entire group.

Reported

1 Business Day

STAT results called out on weekends.

Common Indications

LEUKEMIA - ANEMIA - THROMBOCYTOPENIA - PANCYTOPENIA

CPT Codes

88271(x2), 88275 

Add 88237 CPT for culture of FISH cells - added once per encounter, if necessary, to obtain cells for FISH analysis. This charge is dependent on how much initial sample we receive, the cell count of the sample and what other testing is requested.

Please call 1-866-450-4198 for pricing or with any billing questions.

Collection

Collect

1-3 mL of Bone Marrow or Oncology Blood in a Dark Green top sodium (Na) heparin tube.
    - The preferred choice for specimen collection is listed above, however, if that tube type/container is not available please call the lab for advice on alternative specimen collection choices (513-636-4474).  If the specimen is already collected in a different tube type, send the sample to the lab and we will make every effort to process and result the test.

* PET (paraffin embedded tissue) specimen type (slides or block) can be sent for these FISH Probes

Specify what TYPE of FISH is required on the requisition. If the FISH probe test that you require is not listed on the test requisition, please hand-write the name of the test you want to order in the space provided.

Collection personnel MUST initial specimen container to confirm sample identity.

Minimum Collection Volume

1-3 mL of Bone Marrow or Oncology Blood in a Dark Green top sodium (Na) heparin tube.

If a lesser volume of bone marrow or blood is sent, the laboratory will attempt to perform the test requested.  The laboratory cannot assure either a result and/or the ability to perform repeat testing and/or additional testing if the minimum volumes are not met.

Acceptable Specimen Collect Alternatives

Acceptable:

Bone Marrow/Oncology Blood in sterile culture medium.

Bone Marrow/Oncology Blood in a sterile syringe with NaHep (sodium heparin) added.

For any tube other than those listed in the UNACCEPTABLE CONDITION field (see below) we will attempt to set up and try to obtain FISH results.

Patient Preparation

Please go to "ORDERING" tab to see a complete list of the stand-alone FISH Probes we offer for oncology testing.  Please use our Test Requisition (see link at bottom of next tab) and fill in the FISH probe you require.  If you do not see a FISH probe that you need in the listing, please call our lab to see if we can order it for your testing.

Specimen Preparation

Label tubes with patient name and date of birth (DOB).

Storage/Transport Temperature

Store at room temperature  / Use overnight shipping (protect from temperature extremes, no ice) or call the lab for local courier service (contact laboratory prior to drawing specimen to ensure courier area coverage).

Performing Lab

Cytogenetics Laboratory (513) 636-4474  /  FAX: (513) 636-4373

Performed

6 AM - 12 AM (Monday through Friday, with more limited hours on the weekend)

Unacceptable Conditions

DO NOT SEND if sample has been drawn in a:

CLOT TUBE

NaCitrate Tube

Serum Separation Tube that has been centrifuged (an unspun tube is acceptable)

Ordering

Synonyms

  • 4398422 - FISH : ATM (11q22.3) / p53 (17p13.1)
  • 4623068 - FISH : CDKN2A [p16] (9p21) / CEP 9
  • 5096865 - FISH : 13q14.3 / 13q34
  • 4395187 - FISH : MALT1 (18q21)
  • 4750590 - FISH : T(8 14) MYC/IGH
  • 4750280 - FISH : MONO 7 / DEL 7q (CEP 7 / D7S522)
  • 4388818 - FISH : MALT T(14 18) [IGH / MALT1] [NHL]
  • 4750250 - FISH : T(9 22) [BCR / ABL] - PHILA CHROM
  • 4750260 - FISH : T(15 17) [PML / RARa]
  • 4750700 - FISH : 20q12
  • 4750690 - FISH : MONO 5 / DELETION 5q (EGR1)
  • 4395134 - FISH : ETV6 (12p13)
  • 4750500 - FISH : BCL6 (3q27)
  • 4395176 - FISH : RARa (17q21)
  • 4388766 - FISH : T(11 18) [API2 / MALT1]
  • 4395160 - FISH : IGH (14q32.3)
  • 4750540 - FISH : KMT2A [MLL] (11q23)
  • 4750230 - FISH : XY (BMT ONC)
  • 4750530 - FISH : T(12 21) ETV6 [TEL] / RUNX1[AML1]
  • 4750520 - FISH : T(14 18) [IGH/BCL2] - FOLL. LYMPH
  • 4388795 - FISH : T(14 16) [IGH / MAF]
  • 4398428 - FISH : CEP 12 / 13q14.3 / 13q34
  • 4398415 - FISH : PTEN (10q23)
  • 4750550 - FISH : MYC (8q24)
  • 4750270 - FISH : CEP 8
  • 4398457 - FISH : TP53 (17p13.1)
  • 4395202 - FISH : SYT (18q11.2) - SYNOVIAL SARCOMA
  • 4398327 - FISH : MYCN (2p24.1)
  • 4398464 - FISH : 19p13 / 19q13
  • 4395078 - FISH : ALK (2p23)
  • 4388678 - FISH : T(4 14) [IGH / FGFR3]
  • 4398445 - FISH : D13S319 (13q14.3)
  • 4398338 - FISH : 4q12 (CHIC2, FIP1L1, KIT, LNX, PDGFRA, SCFD2)
  • 5358195 - FISH : EVI1 (3q26.2)
  • 4750680 - FISH : 1p36 /1q25 (ONCOLOGY)
  • 4398338 - FISH : 4q12 - HYPEREOSINOPHILIA
  • 4398451 - FISH : 13q34
  • 4395149 - FISH : FOXO1 [FKHR] (13q14) - RHABDOMYOSARCOMA
  • 4398409 - FISH : 9q34 (ASS)
  • 7291943 - FISH : RCC TFE3 (Xp11.23)
  • 7299733 - FISH : RCC ASPSCR1/PRCC/TFE3 (tricolor DF)
  • 4398397 - FISH : MYB (6q23)
  • 4395217 - FISH : EWSR1 (22q12) - EWING SARCOMA
  • 4395169 - FISH : INVERSION 16 / T(16 16) [CBFB]
  • 7409183 - FISH : USP6 (17p13)
  • 7409180 - FISH : BCL2 (18q21.3)
  • 7291954 - FISH : 1q21 (CKS1B)
  • 7554881 - FISH : BRAF (7q34)
  • 4750480 - FISH : t(11 14) [IGH / CCND1 XT]
  • 4750710 - FISH : t(8 21) RUNX1T1 (ETO)/RUNX1 (AML1)
  • 4399168 - FISH : Aneuploidy 21 (for Oncology)
  • 7530897 - FISH : FUS (16p11)
  • 4750290 - FISH : Miscellaneous FISH (for Oncology)
  • 4398434 - FISH : RB-1 (13q14) (for Oncology)
  • FISH Probe : Somatic (252)

Tests Included

If the FISH probe test that you require is not listed on the test requisition, please hand-write the name of the test you want to order in the space provided.
t(X;1) / t(X;17)
Tricolor Dual Fusion for ASPSCR1/PRCC/TFE3 (RCC)
t(4;14)
LSI IgH (14q32.3)/FGFR3 (4p16) (Dual Fusion)*
t(8;14)
LSI IgH (14q32.3)/ MYC (8q24)/CEP 8 (Dual Fusion)*
t(8;21)
LSI RUNX1T1 (ETO)(8q21.3)/LSI RUNX1 (AML1) (21q22) (Dual Fusion)*
t(9;22)
LSI BCR (22q11.2)/ABL1 (9q34) (Dual Fusion)*
t(11;14)
LSI IgH (14q32.3)/CCND1 (11q13) XT (Dual Fusion)*
t(11;18)
LSI BIRC3 (11q21)/MALT1 (18q21) (Dual Fusion)*
t(12;21)
LSI ETV6 (TEL) (12p13)/LSI RUNX1 (AML1) (21q22) (ES Fusion)*
t(14;16)
LSI IgH (14q32.3)/MAF (16q32) (Dual Fusion)*
t(14;18)
LSI IgH (14q32.3)/LSI BCL2 (18q21) (Dual Fusion)*
t(14;18)
LSI IgH (14q32.3)/LSI MALT1 (18q21) (Dual Fusion)*
t(15;17)
LSI PML (15q22)/RARA (17q21) (Dual Fusion* 



X Chrom  TFE3 (Xp11.23)  - Breakapart (for RCC)
Chrom 1
LSI 1p36 (EGFL3, TP73)/LSI 1q25 (ABL2)
1q21/1p32.3 [CKS1B/CDKN2C] (p18) Amplification/Deletion Probe
Chrom 2
LSI MYCN (2p24.1)/LAF (2q11) (Amplification)
LSI ALK (2p23) (Break Apart)*
Chrom 3
LSI EVI1 (3q26) (TC BA Probe)*
LSI BCL6 (3q27) (Break Apart)*
Chrom 4
LSI 4q12 (SCFD2, FIP1L1/LNX,PDGFRA/KIT) (TC BA Probe)*
Chrom 5
LSI EGR1 (5q31)/D5S23,D5S721 (5p15.2)
PDGFRB (5q32) (Break Apart)*
Chrom 6
LSI MYB (6q23)/CEP 6
Chrom 7
LSI D7S522 (7q31)/CEP 7
EGFR (7p11.2)/CEP 7 (Amplification)
TRG (7p14) (Break Apart)*
TRB (7q34) (Break Apart)*
BRAF (7q34) (Break Apart)*
Chrom 8
LSI MYC (8q24) (Break Apart)*
FGFR1 (8p11) (TC Break Apart/Amplification)*
Chrom 9
LSI CDKN2A [p16] (9p21)/CEP 9
LSI ASS (9q34)
Chrom 10
LSI PTEN (10q32)/CEP 10
Chrom 11
LSI KMT2A [MLL] (11q23) (Break Apart)*
Chrom 11/17
LSI ATM (11q22.3)/LSI p53 (17p13.1)
Chrom 12
LSI CHOP (DDIT3) (12q13) (Break Apart)*
LSI ETV6 (TEL) (12p13) (Break Apart)*
LSI MDM2 (12q15)/CEP 12
Chrom 12/13
LSI D13S319 (13q14.3)/LAMP1 (13q34)/CEP 12
Chrom 13
LSI 13 (RB1) (13q14)
LSI D13S319 (13q14.3)
LSI FOXO1 [FKHR] (13q14) (Break Apart)*
LSI LAMP1 (13q34)
Chrom 14
LSI IgH (14q32.3) (Break Apart)*
LSI TCR alpha/delta (14q11.2) (Break Apart)*
TRAD (14q11.2) (Break Apart)*
Chrom 16
LSI FUS (16p11) (Break Apart)*
LSI CBFB (16q22) (Break Apart)*
CBFB (16q22)/MYH11 (16p13) (Dual Fusion Translocation Probe)*
Chrom 17
LSI TP53 [p53] (17p13.1)
LSI RARA (17q21) (Break Apart)*

Chrom 18
BCL2 (18q21.3) (Break Apart)
LSI MALT1 (18q21) (Break Apart)*
LSI SYT (18q11.2) (Break Apart)*
Chrom 19
LSI 19q13/19p13
LSI E2A (19p13) (Break Apart)*
Chrom 20
LSI D20S108 (20q12)
Chrom 21
LSI 21 (21q22.13-q22.2)
Chrom 22
LSI 22 (BCR) (22q11.2)
LSI EWSR1 (22q12) (Break Apart)*

Sex Mismatched BMT CEP X (SO) / Y (SG)

Methodology

Fluorescence In Situ Hybridization

If multiple single FISH or FISH Panel tests are ordered at the same time, only ONE culture charge is added for the entire group.

Reported

1 Business Day

STAT results called out on weekends.

Result Interpretation

Common Indications

LEUKEMIA - ANEMIA - THROMBOCYTOPENIA - PANCYTOPENIA

Laboratory Personnel Use

CPT Codes

88271(x2), 88275 

Add 88237 CPT for culture of FISH cells - added once per encounter, if necessary, to obtain cells for FISH analysis. This charge is dependent on how much initial sample we receive, the cell count of the sample and what other testing is requested.

Please call 1-866-450-4198 for pricing or with any billing questions.