Collect

Blood or Bone Marrow in 4mL DkGr NaHep. Tissue in Paraffin Block/PET Slides.



Minimum Collection Volume

1 mL Blood or Bone Marrow or 1 Paraffin Tissue Block; or 1 PET/FFPE Cassette; or 3 PET/FFPE/Smear/Touch Slides

Please call the Genetics Lab at 513-636-4474 with minimum sample volume questions on other specimen types or containers. 

If a less than minimum volume is sent, the laboratory will attempt to perform the test requested.  The laboratory cannot assure either a result and/or the ability to perform repeat testing and/or additional testing if the minimum volumes are not met.

Acceptable Specimen Collect Alternatives

ACD (yellow top) and Li Heparin (Lt.Green top) tubes are acceptable.

Bone Marrow/Oncology Blood in sterile culture medium.

Bone Marrow/Oncology Blood in a sterile syringe with NaHep (sodium heparin) added.

Fresh Tissue/Tumor (1cm3) in sterile saline or sterile transport media

PET Slides/Block

Touch Prep Slides

NOTE:  Please send a ""MARKED"" H&E slide with any FFPE/PET specimen

For any tube other than those listed in the UNACCEPTABLE CONDITION field (see below) we will attempt to set up and try to obtain FISH results.

The preferred choice for specimen collection is listed above, however, if that tube type/container is not available please call the lab for advice on alternative specimen collection choices (513-636-4474).  If the specimen is already collected in a different tube type, send the sample to the lab and we will make every effort to process and result the test."

 

Shipping/Handling Instructions (Lab Use Only)

Room Temp. Do NOT spin. Burnet: Call Genetics (6-4474) immediately upon arrival.

External Client Shipping and Handling

Store at room temperature  / Use overnight shipping (protect from temperature extremes, no ice). Do NOT spin tubes.

Stability (from collection to initiation)

SEND TO GENETICS to determine viability of specimen; Call the Genetics Lab at 513-636-4474 for pick up or for any questions or concerns about the specimen.

Notes

Please go to "ORDERING" tab to see a complete list of the stand-alone FISH Probes we offer for oncology testing.  Please use our Test Requisition (see link at bottom of next tab) and fill in the FISH probe you require.  If you do not see a FISH probe that you need in the listing, please call our lab to see if we can order it for your testing.

Collection personnel MUST initial specimen container to confirm sample identity.

Label tubes with patient name and date of birth (DOB).

Performing Lab

Cytogenetics Laboratory 
Phone: (513) 636-4474
Fax: (513) 636-4414

Hours: Dayshift (Monday through Friday)

Unacceptable Conditions

DO NOT SEND if sample has been drawn in a:

CLOT TUBE

NaCitrate Tube

Serum Separation Tube that has been centrifuged (an unspun tube is acceptable)

Synonyms

  • 4398422 - FISH : ATM (11q22.3) / p53 (17p13.1)
  • 4623068 - FISH : CDKN2A [p16] (9p21) / CEP 9
  • 5096865 - FISH : 13q14.3 / 13q34
  • 4395187 - FISH : MALT1 (18q21)
  • 4750590 - FISH : T(8 14) MYC/IGH
  • 4750280 - FISH : MONO 7 / DEL 7q (CEP 7 / D7S522)
  • 4388818 - FISH : MALT T(14 18) [IGH / MALT1] [NHL]
  • 4750250 - FISH : T(9 22) [BCR / ABL] - PHILA CHROM
  • 4750260 - FISH : T(15 17) [PML / RARa]
  • 4750700 - FISH : 20q12
  • 4750690 - FISH : MONO 5 / DELETION 5q (EGR1)
  • 4395134 - FISH : ETV6 (12p13)
  • 4750500 - FISH : BCL6 (3q27)
  • 4395176 - FISH : RARa (17q21)
  • 4395160 - FISH : IGH (14q32.3)
  • 4750540 - FISH : KMT2A [MLL] (11q23)
  • 4750230 - FISH : XY (BMT ONC)
  • 4750530 - FISH : T(12 21) ETV6 [TEL] / RUNX1[AML1]
  • 4750520 - FISH : T(14 18) [IGH/BCL2] - FOLL. LYMPH
  • 4388795 - FISH : T(14 16) [IGH / MAF]
  • 4398428 - FISH : CEP 12 / 13q14.3 / 13q34
  • 4398415 - FISH : PTEN (10q23)
  • 4750550 - FISH : MYC (8q24)
  • 4750270 - FISH : CEP 8
  • 4398457 - FISH : TP53 (17p13.1)
  • 4395202 - FISH : SYT (18q11.2) - SYNOVIAL SARCOMA
  • 4398327 - FISH : MYCN (2p24.1)
  • 4398464 - FISH : 19p13 / 19q13
  • 4395078 - FISH : ALK (2p23)
  • 4388678 - FISH : T(4 14) [IGH / FGFR3]
  • 4398445 - FISH : D13S319 (13q14.3)
  • 4398338 - FISH : 4q12 (CHIC2, FIP1L1, KIT, LNX, PDGFRA, SCFD2)
  • 5358195 - FISH : EVI1 (3q26.2)
  • 4750680 - FISH : 1p36 /1q25 (ONCOLOGY)
  • 4398338 - FISH : 4q12 - HYPEREOSINOPHILIA
  • 4398451 - FISH : 13q34
  • 4395149 - FISH : FOXO1 [FKHR] (13q14) - RHABDOMYOSARCOMA
  • 4398397 - FISH : MYB (6q23)
  • 4395217 - FISH : EWSR1 (22q12) - EWING SARCOMA
  • 4395169 - FISH : INVERSION 16 / T(16 16) [CBFB]
  • 7409183 - FISH : USP6 (17p13)
  • 7409180 - FISH : BCL2 (18q21.3)
  • 7291954 - FISH : 1q21 (CKS1B)
  • 7554881 - FISH : BRAF (7q34)
  • 4750480 - FISH : t(11 14) [IGH / CCND1 XT]
  • 4750710 - FISH : t(8 21) RUNX1T1 (ETO)/RUNX1 (AML1)
  • 4399168 - FISH : Aneuploidy 21 (for Oncology)
  • 7530897 - FISH : FUS (16p11)
  • 4750290 - FISH : Miscellaneous FISH (for Oncology)
  • 4398434 - FISH : RB-1 (13q14) (for Oncology)
  • MISCELLANEOUS FISH PROBE

Tests Included

If the FISH probe test that you require is not listed on the test requisition, please hand-write the name of the test you want to order in the space provided.

t(4;14)
LSI IgH (14q32.3)/FGFR3 (4p16) (Dual Fusion)*
t(8;14)
LSI IgH (14q32.3)/ MYC (8q24)/CEP 8 (Dual Fusion)*
t(8;21)
LSI RUNX1T1 (ETO)(8q21.3)/LSI RUNX1 (AML1) (21q22) (Dual Fusion)*
t(9;22)
LSI BCR (22q11.2)/ABL1 (9q34) (Dual Fusion)*
t(11;14)
LSI IgH (14q32.3)/CCND1 (11q13) XT (Dual Fusion)*
t(12;21)
LSI ETV6 (TEL) (12p13)/LSI RUNX1 (AML1) (21q22) (ES Fusion)*
t(14;16)
LSI IgH (14q32.3)/MAF (16q32) (Dual Fusion)*
t(14;18)
LSI IgH (14q32.3)/LSI BCL2 (18q21) (Dual Fusion)*
t(14;18)
LSI IgH (14q32.3)/LSI MALT1 (18q21) (Dual Fusion)*
t(15;17)
LSI PML (15q22)/RARA (17q21) (Dual Fusion* 


Chrom 1
LSI 1p36 (EGFL3, TP73)/LSI 1q25 (ABL2)
1q21/1p32.3 [CKS1B/CDKN2C] (p18) Amplification/Deletion Probe
Chrom 2
LSI MYCN (2p24.1)/LAF (2q11) (Amplification)
LSI ALK (2p23) (Break Apart)*
Chrom 3
LSI EVI1 (3q26) (TC BA Probe)*
LSI BCL6 (3q27) (Break Apart)*
Chrom 4
LSI 4q12 (SCFD2, FIP1L1/LNX,PDGFRA/KIT) (TC BA Probe)*
Chrom 5
LSI EGR1 (5q31)/D5S23,D5S721 (5p15.2)
PDGFRB (5q32) (Break Apart)*
Chrom 6
LSI MYB (6q23)/CEP 6
Chrom 7
LSI D7S522 (7q31)/CEP 7
EGFR (7p11.2)/CEP 7 (Amplification)
TRG (7p14) (Break Apart)*
TRB (7q34) (Break Apart)*
BRAF (7q34) (Break Apart)*
Chrom 8
LSI MYC (8q24) (Break Apart)*
FGFR1 (8p11) (TC Break Apart/Amplification)*
Chrom 9
LSI CDKN2A [p16] (9p21)/CEP 9
Chrom 10
LSI PTEN (10q32)/CEP 10
Chrom 11
LSI KMT2A [MLL] (11q23) (Break Apart)*
Chrom 11/17
LSI ATM (11q22.3)/LSI p53 (17p13.1)
Chrom 12
LSI CHOP (DDIT3) (12q13) (Break Apart)*
LSI ETV6 (TEL) (12p13) (Break Apart)*
LSI MDM2 (12q15)/CEP 12
Chrom 12/13
LSI D13S319 (13q14.3)/LAMP1 (13q34)/CEP 12
Chrom 13
LSI 13 (RB1) (13q14)
LSI D13S319 (13q14.3)
LSI FOXO1 [FKHR] (13q14) (Break Apart)*
LSI LAMP1 (13q34)
Chrom 14
LSI IgH (14q32.3) (Break Apart)*
LSI TCR alpha/delta (14q11.2) (Break Apart)*
TRAD (14q11.2) (Break Apart)*
Chrom 16
LSI FUS (16p11) (Break Apart)*
LSI CBFB (16q22) (Break Apart)*
CBFB (16q22)/MYH11 (16p13) (Dual Fusion Translocation Probe)*
Chrom 17
LSI TP53 [p53] (17p13.1)
LSI RARA (17q21) (Break Apart)*
Chrom 18
BCL2 (18q21.3) (Break Apart)
LSI MALT1 (18q21) (Break Apart)*
LSI SYT (18q11.2) (Break Apart)*
Chrom 19
LSI 19q13/19p13
LSI E2A (19p13) (Break Apart)*
Chrom 20
LSI D20S108 (20q12)
Chrom 21
LSI 21 (21q22.13-q22.2)
Chrom 22
LSI 22 (BCR) (22q11.2)
LSI EWSR1 (22q12) (Break Apart)*

Sex Mismatched BMT CEP X (SO) / Y (SG)

Methodology

Fluorescence In Situ Hybridization

If multiple single FISH or FISH Panel tests are ordered at the same time, only ONE culture charge is added for the entire group.

Reported

3-5 days

1-3 days for PML/RARa (1st time diagnosis only)

Common Indications

LEUKEMIA - ANEMIA - THROMBOCYTOPENIA - PANCYTOPENIA

CPT Codes

88271(x2), 88275 

Add 88237 CPT for culture of FISH cells - added once per encounter, if necessary, to obtain cells for FISH analysis. This charge is dependent on how much initial sample we receive, the cell count of the sample and what other testing is requested.

Please call 1-866-450-4198 for pricing or with any billing questions.

Collection

Collect

Blood or Bone Marrow in 4mL DkGr NaHep. Tissue in Paraffin Block/PET Slides.



Minimum Collection Volume

1 mL Blood or Bone Marrow or 1 Paraffin Tissue Block; or 1 PET/FFPE Cassette; or 3 PET/FFPE/Smear/Touch Slides

Please call the Genetics Lab at 513-636-4474 with minimum sample volume questions on other specimen types or containers. 

If a less than minimum volume is sent, the laboratory will attempt to perform the test requested.  The laboratory cannot assure either a result and/or the ability to perform repeat testing and/or additional testing if the minimum volumes are not met.

Acceptable Specimen Collect Alternatives

ACD (yellow top) and Li Heparin (Lt.Green top) tubes are acceptable.

Bone Marrow/Oncology Blood in sterile culture medium.

Bone Marrow/Oncology Blood in a sterile syringe with NaHep (sodium heparin) added.

Fresh Tissue/Tumor (1cm3) in sterile saline or sterile transport media

PET Slides/Block

Touch Prep Slides

NOTE:  Please send a ""MARKED"" H&E slide with any FFPE/PET specimen

For any tube other than those listed in the UNACCEPTABLE CONDITION field (see below) we will attempt to set up and try to obtain FISH results.

The preferred choice for specimen collection is listed above, however, if that tube type/container is not available please call the lab for advice on alternative specimen collection choices (513-636-4474).  If the specimen is already collected in a different tube type, send the sample to the lab and we will make every effort to process and result the test."

 

Shipping/Handling Instructions (Lab Use Only)

Room Temp. Do NOT spin. Burnet: Call Genetics (6-4474) immediately upon arrival.

External Client Shipping and Handling

Store at room temperature  / Use overnight shipping (protect from temperature extremes, no ice). Do NOT spin tubes.

Stability (from collection to initiation)

SEND TO GENETICS to determine viability of specimen; Call the Genetics Lab at 513-636-4474 for pick up or for any questions or concerns about the specimen.

Notes

Please go to "ORDERING" tab to see a complete list of the stand-alone FISH Probes we offer for oncology testing.  Please use our Test Requisition (see link at bottom of next tab) and fill in the FISH probe you require.  If you do not see a FISH probe that you need in the listing, please call our lab to see if we can order it for your testing.

Collection personnel MUST initial specimen container to confirm sample identity.

Label tubes with patient name and date of birth (DOB).

Performing Lab

Cytogenetics Laboratory 
Phone: (513) 636-4474
Fax: (513) 636-4414

Hours: Dayshift (Monday through Friday)

Unacceptable Conditions

DO NOT SEND if sample has been drawn in a:

CLOT TUBE

NaCitrate Tube

Serum Separation Tube that has been centrifuged (an unspun tube is acceptable)

Ordering

Synonyms

  • 4398422 - FISH : ATM (11q22.3) / p53 (17p13.1)
  • 4623068 - FISH : CDKN2A [p16] (9p21) / CEP 9
  • 5096865 - FISH : 13q14.3 / 13q34
  • 4395187 - FISH : MALT1 (18q21)
  • 4750590 - FISH : T(8 14) MYC/IGH
  • 4750280 - FISH : MONO 7 / DEL 7q (CEP 7 / D7S522)
  • 4388818 - FISH : MALT T(14 18) [IGH / MALT1] [NHL]
  • 4750250 - FISH : T(9 22) [BCR / ABL] - PHILA CHROM
  • 4750260 - FISH : T(15 17) [PML / RARa]
  • 4750700 - FISH : 20q12
  • 4750690 - FISH : MONO 5 / DELETION 5q (EGR1)
  • 4395134 - FISH : ETV6 (12p13)
  • 4750500 - FISH : BCL6 (3q27)
  • 4395176 - FISH : RARa (17q21)
  • 4395160 - FISH : IGH (14q32.3)
  • 4750540 - FISH : KMT2A [MLL] (11q23)
  • 4750230 - FISH : XY (BMT ONC)
  • 4750530 - FISH : T(12 21) ETV6 [TEL] / RUNX1[AML1]
  • 4750520 - FISH : T(14 18) [IGH/BCL2] - FOLL. LYMPH
  • 4388795 - FISH : T(14 16) [IGH / MAF]
  • 4398428 - FISH : CEP 12 / 13q14.3 / 13q34
  • 4398415 - FISH : PTEN (10q23)
  • 4750550 - FISH : MYC (8q24)
  • 4750270 - FISH : CEP 8
  • 4398457 - FISH : TP53 (17p13.1)
  • 4395202 - FISH : SYT (18q11.2) - SYNOVIAL SARCOMA
  • 4398327 - FISH : MYCN (2p24.1)
  • 4398464 - FISH : 19p13 / 19q13
  • 4395078 - FISH : ALK (2p23)
  • 4388678 - FISH : T(4 14) [IGH / FGFR3]
  • 4398445 - FISH : D13S319 (13q14.3)
  • 4398338 - FISH : 4q12 (CHIC2, FIP1L1, KIT, LNX, PDGFRA, SCFD2)
  • 5358195 - FISH : EVI1 (3q26.2)
  • 4750680 - FISH : 1p36 /1q25 (ONCOLOGY)
  • 4398338 - FISH : 4q12 - HYPEREOSINOPHILIA
  • 4398451 - FISH : 13q34
  • 4395149 - FISH : FOXO1 [FKHR] (13q14) - RHABDOMYOSARCOMA
  • 4398397 - FISH : MYB (6q23)
  • 4395217 - FISH : EWSR1 (22q12) - EWING SARCOMA
  • 4395169 - FISH : INVERSION 16 / T(16 16) [CBFB]
  • 7409183 - FISH : USP6 (17p13)
  • 7409180 - FISH : BCL2 (18q21.3)
  • 7291954 - FISH : 1q21 (CKS1B)
  • 7554881 - FISH : BRAF (7q34)
  • 4750480 - FISH : t(11 14) [IGH / CCND1 XT]
  • 4750710 - FISH : t(8 21) RUNX1T1 (ETO)/RUNX1 (AML1)
  • 4399168 - FISH : Aneuploidy 21 (for Oncology)
  • 7530897 - FISH : FUS (16p11)
  • 4750290 - FISH : Miscellaneous FISH (for Oncology)
  • 4398434 - FISH : RB-1 (13q14) (for Oncology)
  • MISCELLANEOUS FISH PROBE

Tests Included

If the FISH probe test that you require is not listed on the test requisition, please hand-write the name of the test you want to order in the space provided.

t(4;14)
LSI IgH (14q32.3)/FGFR3 (4p16) (Dual Fusion)*
t(8;14)
LSI IgH (14q32.3)/ MYC (8q24)/CEP 8 (Dual Fusion)*
t(8;21)
LSI RUNX1T1 (ETO)(8q21.3)/LSI RUNX1 (AML1) (21q22) (Dual Fusion)*
t(9;22)
LSI BCR (22q11.2)/ABL1 (9q34) (Dual Fusion)*
t(11;14)
LSI IgH (14q32.3)/CCND1 (11q13) XT (Dual Fusion)*
t(12;21)
LSI ETV6 (TEL) (12p13)/LSI RUNX1 (AML1) (21q22) (ES Fusion)*
t(14;16)
LSI IgH (14q32.3)/MAF (16q32) (Dual Fusion)*
t(14;18)
LSI IgH (14q32.3)/LSI BCL2 (18q21) (Dual Fusion)*
t(14;18)
LSI IgH (14q32.3)/LSI MALT1 (18q21) (Dual Fusion)*
t(15;17)
LSI PML (15q22)/RARA (17q21) (Dual Fusion* 


Chrom 1
LSI 1p36 (EGFL3, TP73)/LSI 1q25 (ABL2)
1q21/1p32.3 [CKS1B/CDKN2C] (p18) Amplification/Deletion Probe
Chrom 2
LSI MYCN (2p24.1)/LAF (2q11) (Amplification)
LSI ALK (2p23) (Break Apart)*
Chrom 3
LSI EVI1 (3q26) (TC BA Probe)*
LSI BCL6 (3q27) (Break Apart)*
Chrom 4
LSI 4q12 (SCFD2, FIP1L1/LNX,PDGFRA/KIT) (TC BA Probe)*
Chrom 5
LSI EGR1 (5q31)/D5S23,D5S721 (5p15.2)
PDGFRB (5q32) (Break Apart)*
Chrom 6
LSI MYB (6q23)/CEP 6
Chrom 7
LSI D7S522 (7q31)/CEP 7
EGFR (7p11.2)/CEP 7 (Amplification)
TRG (7p14) (Break Apart)*
TRB (7q34) (Break Apart)*
BRAF (7q34) (Break Apart)*
Chrom 8
LSI MYC (8q24) (Break Apart)*
FGFR1 (8p11) (TC Break Apart/Amplification)*
Chrom 9
LSI CDKN2A [p16] (9p21)/CEP 9
Chrom 10
LSI PTEN (10q32)/CEP 10
Chrom 11
LSI KMT2A [MLL] (11q23) (Break Apart)*
Chrom 11/17
LSI ATM (11q22.3)/LSI p53 (17p13.1)
Chrom 12
LSI CHOP (DDIT3) (12q13) (Break Apart)*
LSI ETV6 (TEL) (12p13) (Break Apart)*
LSI MDM2 (12q15)/CEP 12
Chrom 12/13
LSI D13S319 (13q14.3)/LAMP1 (13q34)/CEP 12
Chrom 13
LSI 13 (RB1) (13q14)
LSI D13S319 (13q14.3)
LSI FOXO1 [FKHR] (13q14) (Break Apart)*
LSI LAMP1 (13q34)
Chrom 14
LSI IgH (14q32.3) (Break Apart)*
LSI TCR alpha/delta (14q11.2) (Break Apart)*
TRAD (14q11.2) (Break Apart)*
Chrom 16
LSI FUS (16p11) (Break Apart)*
LSI CBFB (16q22) (Break Apart)*
CBFB (16q22)/MYH11 (16p13) (Dual Fusion Translocation Probe)*
Chrom 17
LSI TP53 [p53] (17p13.1)
LSI RARA (17q21) (Break Apart)*
Chrom 18
BCL2 (18q21.3) (Break Apart)
LSI MALT1 (18q21) (Break Apart)*
LSI SYT (18q11.2) (Break Apart)*
Chrom 19
LSI 19q13/19p13
LSI E2A (19p13) (Break Apart)*
Chrom 20
LSI D20S108 (20q12)
Chrom 21
LSI 21 (21q22.13-q22.2)
Chrom 22
LSI 22 (BCR) (22q11.2)
LSI EWSR1 (22q12) (Break Apart)*

Sex Mismatched BMT CEP X (SO) / Y (SG)

Methodology

Fluorescence In Situ Hybridization

If multiple single FISH or FISH Panel tests are ordered at the same time, only ONE culture charge is added for the entire group.

Reported

3-5 days

1-3 days for PML/RARa (1st time diagnosis only)
Result Interpretation

Common Indications

LEUKEMIA - ANEMIA - THROMBOCYTOPENIA - PANCYTOPENIA

Laboratory Personnel Use

CPT Codes

88271(x2), 88275 

Add 88237 CPT for culture of FISH cells - added once per encounter, if necessary, to obtain cells for FISH analysis. This charge is dependent on how much initial sample we receive, the cell count of the sample and what other testing is requested.

Please call 1-866-450-4198 for pricing or with any billing questions.

×
Learn More Remind Me Later Dismiss