Collect

20-25 mL of STERILE amniotic fluid in STERILE tubes (protect from light by using dark tubes or placing tubes in a dark bag).
-The preferred choice for specimen collection is listed above, however, if that tube type/container is not available please call the lab for advice on alternative specimen collection choices.  If the specimen is already collected in a different tube type, send the sample to the lab and we will make every effort to process and result the test.

Collection personnel MUST initial specimen container to confirm sample identity.

Minimum Collection Volume

To achieve a turn around time (TAT) of 7 days, at least 12-15 ml of amniotic fluid must be received.

If a lesser volume of amniotic fluid is sent, the laboratory will attempt to perform the test requested.  The laboratory cannot assure either a result and/or the ability to perform repeat testing and/or additional testing if the minimum volumes are not met.

Specimen Preparation

Label tubes with patient name and date of birth (DOB).

For twin pregnancies, label tubes with the mother's name and "TWIN A" on one fetal sample and "TWIN B" on the second fetal sample.

Storage/Transport Temperature

Protect from light.  Store at room temperature.

Use overnight shipping (protect from temperature extremes, no ice) or call the lab for local courier service (contact laboratory prior to drawing specimen to ensure courier area coverage).

Performing Lab

Cytogenetics Laboratory (513) 636-4474  /  FAX: (513) 636-4373

Performed

6 AM - 12 AM (Monday through Friday, with more limited hours on the weekend)

Synonyms

  • KARYOTYPE - AMNIOTIC FLUID CHROMOSOME STUDY
  • AMNIOTIC FLUID CHROMOSOME ANALYSIS
  • 222 - CHROMOSOME ANALYSIS : AMNIOTIC FLUID

Reflex Testing

Alpha-Fetoprotein (AFP) testing on the amniotic fluid is recommended if specimen is between 13-23.6 wks gestation.  Acetyl Cholinesterase (AChE) testing is ordered if AFP is found to be abnormal or if gestational age is over 22 wks. 

Methodology

Cell culture followed by cytogenetic analysis of fetal metaphase chromosomes.

Reported

7-10 Days

CPT Codes

88235(x2), 88269, 88280, 88285 (for chromosome analysis)

82106 for AFP testing

82013 for AChE testing

Please call 1-866-450-4198 for pricing or with any billing questions.

Lab Use Only

PROTECT FROM LIGHT/ ROOM TEMPERATURE/ CALL 6-4474 FOR PICK UP as soon as possible

Collection

Collect

20-25 mL of STERILE amniotic fluid in STERILE tubes (protect from light by using dark tubes or placing tubes in a dark bag).
-The preferred choice for specimen collection is listed above, however, if that tube type/container is not available please call the lab for advice on alternative specimen collection choices.  If the specimen is already collected in a different tube type, send the sample to the lab and we will make every effort to process and result the test.

Collection personnel MUST initial specimen container to confirm sample identity.

Minimum Collection Volume

To achieve a turn around time (TAT) of 7 days, at least 12-15 ml of amniotic fluid must be received.

If a lesser volume of amniotic fluid is sent, the laboratory will attempt to perform the test requested.  The laboratory cannot assure either a result and/or the ability to perform repeat testing and/or additional testing if the minimum volumes are not met.

Specimen Preparation

Label tubes with patient name and date of birth (DOB).

For twin pregnancies, label tubes with the mother's name and "TWIN A" on one fetal sample and "TWIN B" on the second fetal sample.

Storage/Transport Temperature

Protect from light.  Store at room temperature.

Use overnight shipping (protect from temperature extremes, no ice) or call the lab for local courier service (contact laboratory prior to drawing specimen to ensure courier area coverage).

Performing Lab

Cytogenetics Laboratory (513) 636-4474  /  FAX: (513) 636-4373

Performed

6 AM - 12 AM (Monday through Friday, with more limited hours on the weekend)

Ordering

Synonyms

  • KARYOTYPE - AMNIOTIC FLUID CHROMOSOME STUDY
  • AMNIOTIC FLUID CHROMOSOME ANALYSIS
  • 222 - CHROMOSOME ANALYSIS : AMNIOTIC FLUID

Reflex Testing

Alpha-Fetoprotein (AFP) testing on the amniotic fluid is recommended if specimen is between 13-23.6 wks gestation.  Acetyl Cholinesterase (AChE) testing is ordered if AFP is found to be abnormal or if gestational age is over 22 wks. 

Methodology

Cell culture followed by cytogenetic analysis of fetal metaphase chromosomes.

Reported

7-10 Days

Result Interpretation
Laboratory Personnel Use

CPT Codes

88235(x2), 88269, 88280, 88285 (for chromosome analysis)

82106 for AFP testing

82013 for AChE testing

Please call 1-866-450-4198 for pricing or with any billing questions.

Lab Use Only

PROTECT FROM LIGHT/ ROOM TEMPERATURE/ CALL 6-4474 FOR PICK UP as soon as possible