Collect

Blood in 3mL Lav EDTA. Received in lab within 4 hour time of collection, deliver STAT to lab. Should be kept refrigerated. Collect only Mon-Fri. Must be received to Burnet lab by 1400 if collected on Fri.

Minimum Collection Volume

0.5ml EDTA (1 full lavender microtainer) 

Shipping/Handling Instructions (Lab Use Only)

Critical: Refrigerate

External Client Shipping and Handling

Sample must be refrigerated within 4 hours of collection.  Must be received in testing laboratory within 24 hours.Ship with 2-3 frozen ice packs. Put a barrier between the ice packs and the specimen. Avoid having the specimen directly touching the Ice pack. Should be shipped in an insulated box. Normal Travel Control/Donor should be shipped with the patient

Stability (from collection to initiation)

Refrigerate 48 hours.

Notes

Testing performed Mon.- Fri. 

Performing Lab

CBDI Erythrocyte Diagnostic Laboratory 513-636-4234

Units of Measure

mmHg

Ordering Recommendations

The Oxygen Dissociation (p50) assay is used to aid in the diagnosis of hemoglobin variants that cause abnormal hemoglobin oxygen affinity.

Synonyms

  • OXYGEN DISSOCIATION P50, ERYTHROCYTES
  • P50
  • OXYGEN AFFINITY
  • OXYHEMOGLOBIN DISSOCIATION
  • O2 AFFINITY

Tests Included

Oxygen Dissociation p50

Methodology

Hemox Analyzer (TCS Scientific, New Hope, PA): uses dual wavelength spectrophtometry to measure oxygen saturation of hemoglobin and a Clark electrode to assess oxygen partial pressure in millimeters of mercury. The p50 value is determined as the partial pressure of oxygen where hemoglobin is 50% oxygenated.

Reported

3 days

Reference Interval

EDL Hemox Analyzers:
Patients > 6 months old  22.54- 28.15 mm Hg

For Patients <= 6 months old, a reference range for this age group has not been established due to developmental hemoglobin switching that occurs during the first several months of life. Higher amounts of fetal Hb (HbF) are associated with lower p50 values. The p50 of HbF in isolation is approximately 19 mm Hg.

CPT Codes

82820
Collection

Collect

Blood in 3mL Lav EDTA. Received in lab within 4 hour time of collection, deliver STAT to lab. Should be kept refrigerated. Collect only Mon-Fri. Must be received to Burnet lab by 1400 if collected on Fri.

Minimum Collection Volume

0.5ml EDTA (1 full lavender microtainer) 

Shipping/Handling Instructions (Lab Use Only)

Critical: Refrigerate

External Client Shipping and Handling

Sample must be refrigerated within 4 hours of collection.  Must be received in testing laboratory within 24 hours.Ship with 2-3 frozen ice packs. Put a barrier between the ice packs and the specimen. Avoid having the specimen directly touching the Ice pack. Should be shipped in an insulated box. Normal Travel Control/Donor should be shipped with the patient

Stability (from collection to initiation)

Refrigerate 48 hours.

Notes

Testing performed Mon.- Fri. 

Performing Lab

CBDI Erythrocyte Diagnostic Laboratory 513-636-4234

Units of Measure

mmHg

Ordering

Ordering Recommendations

The Oxygen Dissociation (p50) assay is used to aid in the diagnosis of hemoglobin variants that cause abnormal hemoglobin oxygen affinity.

Synonyms

  • OXYGEN DISSOCIATION P50, ERYTHROCYTES
  • P50
  • OXYGEN AFFINITY
  • OXYHEMOGLOBIN DISSOCIATION
  • O2 AFFINITY

Tests Included

Oxygen Dissociation p50

Methodology

Hemox Analyzer (TCS Scientific, New Hope, PA): uses dual wavelength spectrophtometry to measure oxygen saturation of hemoglobin and a Clark electrode to assess oxygen partial pressure in millimeters of mercury. The p50 value is determined as the partial pressure of oxygen where hemoglobin is 50% oxygenated.

Reported

3 days
Result Interpretation

Reference Interval

EDL Hemox Analyzers:
Patients > 6 months old  22.54- 28.15 mm Hg

For Patients <= 6 months old, a reference range for this age group has not been established due to developmental hemoglobin switching that occurs during the first several months of life. Higher amounts of fetal Hb (HbF) are associated with lower p50 values. The p50 of HbF in isolation is approximately 19 mm Hg.
Laboratory Personnel Use

CPT Codes

82820