15 mL of whole blood in (3) 5 mL EDTA (lavender top) tubes, must call ahead to (513) 636-4474 for scheduling testing.
Collection personnel MUST initial specimen container to confirm sample identity.
Minimum Collection Volume
15 mL whole blood
The laboratory cannot assure a result if the minimum volumes are not met.
Acceptable Specimen Collect Alternatives
The laboratory can obtain results for this test using different collection tube types. For best results, send one of the collection tube type listed above. If only an alternate collection tube type is available, please call the laboratory at (513) 636-4474 for more information on how to obtain and/or handle any alternate tube types and whether or not they are acceptable for this testing.
Patient Preparation
The cell sorting MUST be scheduled with the CCHMC GENETICS Laboratory ahead of time.
Please call: (513)636-4474to schedule.
Storage/Transport Temperature
Store at room temperature / Use overnight shipping (protect from temperature extremes, no ice)
HOURS of OPERATION: 6 AM - 12 AM (Monday through Friday, with more limited hours on the weekend)
Performed
Monday through Friday - MUST CALL (513) 636-4474 TO PRE-ARRANGE FOR TESTING
Unacceptable Conditions
No name on specimen container
Remarks
OHC TEST ID# : 4750860
Ordering Recommendations
This test is performed on separated cell populations from the HOST (patient) POST bone marrow transplant sample for SAME SEX donor and host.
The FISH : XY (FLOW SORTED CELLS) testing is usually used for patients with OPPOSITE SEX donor and host.
Note: Specimens from the bone marrow patient (post-transplant), donor and from the patient pre-transplant are required for performing the engraftment testing
Synonyms
BME BY STR - SUB CELL
ENGRAFTMENT STUDY - SUB CELL
SORTED ENGRAFTMENT
551 - Bone Marrow Engraftment Sub Cell Type
Methodology
Lymphocyte subsets are magnetically isolated using positive cell selection. Cell types are then analyzed separately using polymerase chain reaction (PCR) amplification of 24, highly variable, short tandem repeats (STRs) followed by capillary electrophoresis for size discrimination to determine the various alleles at the 24 individual loci as well as chromosome X and Y specific products.
Please call 1-866-450-4198 for pricing or with any billing questions.
Collection
Collect
15 mL of whole blood in (3) 5 mL EDTA (lavender top) tubes, must call ahead to (513) 636-4474 for scheduling testing.
Collection personnel MUST initial specimen container to confirm sample identity.
Minimum Collection Volume
15 mL whole blood
The laboratory cannot assure a result if the minimum volumes are not met.
Acceptable Specimen Collect Alternatives
The laboratory can obtain results for this test using different collection tube types. For best results, send one of the collection tube type listed above. If only an alternate collection tube type is available, please call the laboratory at (513) 636-4474 for more information on how to obtain and/or handle any alternate tube types and whether or not they are acceptable for this testing.
Patient Preparation
The cell sorting MUST be scheduled with the CCHMC GENETICS Laboratory ahead of time.
Please call: (513)636-4474to schedule.
Storage/Transport Temperature
Store at room temperature / Use overnight shipping (protect from temperature extremes, no ice)
HOURS of OPERATION: 6 AM - 12 AM (Monday through Friday, with more limited hours on the weekend)
Performed
Monday through Friday - MUST CALL (513) 636-4474 TO PRE-ARRANGE FOR TESTING
Unacceptable Conditions
No name on specimen container
Remarks
OHC TEST ID# : 4750860
Ordering
Ordering Recommendations
This test is performed on separated cell populations from the HOST (patient) POST bone marrow transplant sample for SAME SEX donor and host.
The FISH : XY (FLOW SORTED CELLS) testing is usually used for patients with OPPOSITE SEX donor and host.
Note: Specimens from the bone marrow patient (post-transplant), donor and from the patient pre-transplant are required for performing the engraftment testing
Synonyms
BME BY STR - SUB CELL
ENGRAFTMENT STUDY - SUB CELL
SORTED ENGRAFTMENT
551 - Bone Marrow Engraftment Sub Cell Type
Methodology
Lymphocyte subsets are magnetically isolated using positive cell selection. Cell types are then analyzed separately using polymerase chain reaction (PCR) amplification of 24, highly variable, short tandem repeats (STRs) followed by capillary electrophoresis for size discrimination to determine the various alleles at the 24 individual loci as well as chromosome X and Y specific products.
