3-5 mL Whole Blood or 5-10 mL Bone Marrow in Lavender top tube (EDTA).
Collection personnel MUST initial specimen container to confirm sample identity.
Minimum Collection Volume
3 mL whole blood or 5 mL bone marrow in lavender top (EDTA)
If a lesser volume of blood or bone marrow is sent, the laboratory will attempt to perform the test requested. The laboratory cannot assure either a result and/or the ability to perform repeat testing and/or additional testing if the minimum volumes are not met.
Specimen Preparation
Do not centrifuge (spin) tube.
Storage/Transport Temperature
Store at room temperature / Use overnight shipping (protect from temperature extremes, no ice)
HOURS of OPERATION: 6 AM - 12 AM (Monday through Friday, with more limited hours on the weekend)
Unacceptable Conditions
No name on specimen container / Collected in sodium heparin (green top) collection tube
Remarks
OHC TEST ID# : 4783470
Synonyms
JAK2 SNP (V617F site specific within EXON 14 ONLY)
JAK2 Mutation Analysis
579 - JAK2 SNP ANALYSIS
Methodology
We use the JAK2 MutaQuant™ Assay from Ipsogen. This highly sensitive approach involves the use of a SNP specific forward primer resulting in the selective amplification of mutant or wild type allele. The fluorescent labeled products are easily detectable on a real-time PCR instrument. The quantities of normal and mutated JAK2 copies are measured using a standard curve and the relative percentage of genes that possess this mutation detected (%V617) is reported.
Please call 1-866-450-4198 for pricing or with any billing questions.
Collection
Collect
3-5 mL Whole Blood or 5-10 mL Bone Marrow in Lavender top tube (EDTA).
Collection personnel MUST initial specimen container to confirm sample identity.
Minimum Collection Volume
3 mL whole blood or 5 mL bone marrow in lavender top (EDTA)
If a lesser volume of blood or bone marrow is sent, the laboratory will attempt to perform the test requested. The laboratory cannot assure either a result and/or the ability to perform repeat testing and/or additional testing if the minimum volumes are not met.
Specimen Preparation
Do not centrifuge (spin) tube.
Storage/Transport Temperature
Store at room temperature / Use overnight shipping (protect from temperature extremes, no ice)
HOURS of OPERATION: 6 AM - 12 AM (Monday through Friday, with more limited hours on the weekend)
Unacceptable Conditions
No name on specimen container / Collected in sodium heparin (green top) collection tube
Remarks
OHC TEST ID# : 4783470
Ordering
Synonyms
JAK2 SNP (V617F site specific within EXON 14 ONLY)
JAK2 Mutation Analysis
579 - JAK2 SNP ANALYSIS
Methodology
We use the JAK2 MutaQuant™ Assay from Ipsogen. This highly sensitive approach involves the use of a SNP specific forward primer resulting in the selective amplification of mutant or wild type allele. The fluorescent labeled products are easily detectable on a real-time PCR instrument. The quantities of normal and mutated JAK2 copies are measured using a standard curve and the relative percentage of genes that possess this mutation detected (%V617) is reported.
