Collect

1 mL blood in EDTA (Lavender)

Minimum Collection Volume

0.5 mL EDTA (Lavender)

Acceptable Specimen Collect Alternatives

Sodium Heparin (dark green) may also be accepted, however a concurrent CBC and differential are required in order to report absolute counts

Unacceptable Conditions

Frozen, clotted, hemolyzed or centrifuged specimens. Specimen collected in an unacceptable anticoagulant. Specimens received greater than 24 hours after collection.

Storage/Transport Temperature

AMBIENT

Specimen Preparation

Room Temp. Must be received to Burnet by 1400 if collected on Fri.

Stability (from collection to initiation)

Ambient: 24 hours / Frozen: Unacceptable / Refrigerated: Unacceptable.

Performed

Collect only Mon-Fri. Must be received to Burnet by 1400 if collected on Fri.

Performing Lab

CBDI DIL 513-636-4685

Units of Measure

%, cells/mcL

Methodology

Flow Cytometry

Reported

2 days

Tests Included

% and absolute count for the lymphocyte subpopulations: Total T cells (CD3), T helper cells (CD4), T cytotoxic cells (CD8), B cells (CD19), and NK cells (CD16/CD56); CD4:CD8 ratio

Synonyms

  • T, B & NK Cell Enumeration
  • lymphocyte subpopulations
  • CD4:CD8 testing
  • T cell Subpopulation

CPT Codes

86360, 86359, 86355, 86357

Acceptable Specimen Collect Alternatives

Sodium Heparin (dark green) may also be accepted, however a concurrent CBC and differential are required in order to report absolute counts

Collection

Collect

1 mL blood in EDTA (Lavender)

Minimum Collection Volume

0.5 mL EDTA (Lavender)

Acceptable Specimen Collect Alternatives

Sodium Heparin (dark green) may also be accepted, however a concurrent CBC and differential are required in order to report absolute counts

Unacceptable Conditions

Frozen, clotted, hemolyzed or centrifuged specimens. Specimen collected in an unacceptable anticoagulant. Specimens received greater than 24 hours after collection.

Storage/Transport Temperature

AMBIENT

Specimen Preparation

Room Temp. Must be received to Burnet by 1400 if collected on Fri.

Stability (from collection to initiation)

Ambient: 24 hours / Frozen: Unacceptable / Refrigerated: Unacceptable.

Performed

Collect only Mon-Fri. Must be received to Burnet by 1400 if collected on Fri.

Performing Lab

CBDI DIL 513-636-4685

Units of Measure

%, cells/mcL
Ordering

Methodology

Flow Cytometry

Reported

2 days

Tests Included

% and absolute count for the lymphocyte subpopulations: Total T cells (CD3), T helper cells (CD4), T cytotoxic cells (CD8), B cells (CD19), and NK cells (CD16/CD56); CD4:CD8 ratio

Synonyms

  • T, B & NK Cell Enumeration
  • lymphocyte subpopulations
  • CD4:CD8 testing
  • T cell Subpopulation
Result Interpretation
Laboratory Personnel Use

CPT Codes

86360, 86359, 86355, 86357

Acceptable Specimen Collect Alternatives

Sodium Heparin (dark green) may also be accepted, however a concurrent CBC and differential are required in order to report absolute counts