3-5 mL Blood in 5 mL Lav top (EDTA)
or
2-5 mcL DNA in1.5 mL Epdrf tube TE buffer
For convenience to the family and the ordering physicians (ease of ordering, calling out results, and utilizing the genetic results), we offer parental comparative analysis with the RASopathy/Noonan Panel. For parental comparative analysis, please order the RASopathy/Noonan Panel on patient and parents. Parental samples will be analyzed if the proband has a positive or inconclusive result. If the result is negative, the DNA for the parents will be stored for future testing. We require the proband and parents have their blood drawn and sent to the lab at the same time. If logistics prohibits this, the parental blood should be sent no longer than a week after the proband.
If DNA is sent as a specimen for this assay, the DNA must have been extracted at a CLIA and/or CAP accredited laboratory.
Minimum Collection Volume
1 mL Blood in 5 mL Lavender top (EDTA)
2 mcL DNA in1.5 mL Epdrf tube TE buffer
Acceptable Specimen Collect Alternatives
25 cm Cells in Flask (cm2) filled with media
Saliva, use saliva collection kit
2 cytobrushes
0.5 mL amniotic fluid in Sterile Cntr
Stability (from collection to initiation)
Blood - Ambient: 72 hours; Refrigerated: 1 week; Frozen: Unacceptable DNA - stable in Epdrf Tube w/TE buffer Cells - Ambient: 24 hours; Refrigerated: Unacceptable; Frozen: Unacceptable Amniotic Fluid- Ambient: 24 hours, Lab must receive sample within 24 hours of collection, protect from light using dark tubes or placing tubes in a dark bag. Do not refrigerate or freeze.
Specimen Preparation
Whole blood samples, 3-5 mL, should be collected in an EDTA (purple top) tube and stored at 4°C until shipped.Amniotic Fluid- Lab must receive sample within 24 hours of collection, protect from light using dark tubes or placing tubes in a dark bag. All samples should be clearly labeled with the patient’s name and date of birth or two other unique identifiers.
Storage/Transport Temperature
All samples for genetic testing may be shipped at room temperature by overnight express carrier, such as FedEx, UPS, or DHL to arrive Monday through Friday.All specimens should be appropriately packaged to avoid crushing, breakage, or leakage.
3-5 mL Blood in 5 mL Lav top (EDTA)
or
2-5 mcL DNA in1.5 mL Epdrf tube TE buffer
For convenience to the family and the ordering physicians (ease of ordering, calling out results, and utilizing the genetic results), we offer parental comparative analysis with the RASopathy/Noonan Panel. For parental comparative analysis, please order the RASopathy/Noonan Panel on patient and parents. Parental samples will be analyzed if the proband has a positive or inconclusive result. If the result is negative, the DNA for the parents will be stored for future testing. We require the proband and parents have their blood drawn and sent to the lab at the same time. If logistics prohibits this, the parental blood should be sent no longer than a week after the proband.
If DNA is sent as a specimen for this assay, the DNA must have been extracted at a CLIA and/or CAP accredited laboratory.
Minimum Collection Volume
1 mL Blood in 5 mL Lavender top (EDTA)
2 mcL DNA in1.5 mL Epdrf tube TE buffer
Acceptable Specimen Collect Alternatives
25 cm Cells in Flask (cm2) filled with media
Saliva, use saliva collection kit
2 cytobrushes
0.5 mL amniotic fluid in Sterile Cntr
Stability (from collection to initiation)
Blood - Ambient: 72 hours; Refrigerated: 1 week; Frozen: Unacceptable DNA - stable in Epdrf Tube w/TE buffer Cells - Ambient: 24 hours; Refrigerated: Unacceptable; Frozen: Unacceptable Amniotic Fluid- Ambient: 24 hours, Lab must receive sample within 24 hours of collection, protect from light using dark tubes or placing tubes in a dark bag. Do not refrigerate or freeze.
Specimen Preparation
Whole blood samples, 3-5 mL, should be collected in an EDTA (purple top) tube and stored at 4°C until shipped.Amniotic Fluid- Lab must receive sample within 24 hours of collection, protect from light using dark tubes or placing tubes in a dark bag. All samples should be clearly labeled with the patient’s name and date of birth or two other unique identifiers.
Storage/Transport Temperature
All samples for genetic testing may be shipped at room temperature by overnight express carrier, such as FedEx, UPS, or DHL to arrive Monday through Friday.All specimens should be appropriately packaged to avoid crushing, breakage, or leakage.
