15 mL whole blood in (3) 5 mL EDTA (lavender top) tubes, must call ahead to (513) 636-4474 for scheduling testing.
Collection personnel MUST initial specimen container to confirm sample identity.
Minimum Collection Volume
15 mL whole blood
The laboratory cannot assure a result if the minimum volumes are not met.
Acceptable Specimen Collect Alternatives
The laboratory can obtain results for this test using different collection tube types. For best results, send one of the collection tube type listed above. If only an alternate collection tube type is available, please call the laboratory at (513) 636-4474 for more information on how to obtain and/or handle any alternate tube types and whether or not they are acceptable for this testing.
Patient Preparation
The cell sorting MUST be scheduled with the CCHMC GENETICS Laboratory ahead of time.
Please call: (513)636-4474to schedule.
Storage/Transport Temperature
Store at room temperature / Use overnight shipping (protect from temperature extremes, no ice)
Monday through Friday - MUST CALL (513) 636-4474 TO PRE-ARRANGE FOR TESTING
Ordering Recommendations
This test is usually performed on separated cell populations from the HOST (patient) POST bone marrow transplant sample for OPPOSITE SEX donor and host.
Use the Bone Marrow Engraftment by STR - Sub Cell testing for patients with SAME SEX donor and host.
Please indicate the types of cells to separate and the order of preference for the types of separated cells.
Methodology
Lymphocyte subsets are magnetically isolated using positive cell selection. Cell types are then analyzed separately using fluorescence in situ hybridization (FISH).
15 mL whole blood in (3) 5 mL EDTA (lavender top) tubes, must call ahead to (513) 636-4474 for scheduling testing.
Collection personnel MUST initial specimen container to confirm sample identity.
Minimum Collection Volume
15 mL whole blood
The laboratory cannot assure a result if the minimum volumes are not met.
Acceptable Specimen Collect Alternatives
The laboratory can obtain results for this test using different collection tube types. For best results, send one of the collection tube type listed above. If only an alternate collection tube type is available, please call the laboratory at (513) 636-4474 for more information on how to obtain and/or handle any alternate tube types and whether or not they are acceptable for this testing.
Patient Preparation
The cell sorting MUST be scheduled with the CCHMC GENETICS Laboratory ahead of time.
Please call: (513)636-4474to schedule.
Storage/Transport Temperature
Store at room temperature / Use overnight shipping (protect from temperature extremes, no ice)
Monday through Friday - MUST CALL (513) 636-4474 TO PRE-ARRANGE FOR TESTING
Ordering
Ordering Recommendations
This test is usually performed on separated cell populations from the HOST (patient) POST bone marrow transplant sample for OPPOSITE SEX donor and host.
Use the Bone Marrow Engraftment by STR - Sub Cell testing for patients with SAME SEX donor and host.
Please indicate the types of cells to separate and the order of preference for the types of separated cells.
Methodology
Lymphocyte subsets are magnetically isolated using positive cell selection. Cell types are then analyzed separately using fluorescence in situ hybridization (FISH).
