Collect

Blood or Bone Marrow in 5-3mL Lav EDTA. MUST schedule with Genetics (513) 636-4474 before collection on only Mon-Fri. Must be received to Burnet lab by 1400 if collected on Fri.


Minimum Collection Volume

Blood:  12 mL 
Bone Marrow: 3 mL 

The laboratory cannot assure a result if the minimum volumes are not met.  Contact GENETICS for more information on minimum volumes.

Acceptable Specimen Collect Alternatives

The laboratory can obtain results for this test using different collection tube types. For best results, send one of the collection tube type listed above.  If only an alternate collection tube type is available, please call the laboratory at (513) 636-4474 for more information on how to obtain and/or handle any alternate tube types and whether or not they are acceptable for this testing.

Shipping/Handling Instructions (Lab Use Only)

Room Temperature. Do NOT spin. Burnet: Call GENETICS (6-4474) immediately upon arrival.

External Client Shipping and Handling

Store at room temperature  / Use overnight shipping (protect from temperature extremes, no ice) or call the lab for local courier service (contact laboratory prior to drawing specimen to ensure courier area coverage).  Do NOT spin tubes.

Stability (from collection to initiation)

Room Temp 72 hrs. 

Notes

The cell sorting MUST be scheduled with the CCHMC GENETICS Laboratory ahead of time. Please call:  (513)636-4474  to schedule. Collection personnel MUST initial specimen container to confirm sample identity.

Performing Lab

Cytogenetics Laboratory 
Phone: (513) 636-4474
Fax: (513) 636-4414

Hours: Dayshift (Monday through Friday)

Ordering Recommendations

Please indicate what types of cells require separation and list the PRIORITY of cell types wanted, in case there is not enough sample to perform separation for all cell types listed. Possible Cell Types:  B-cells; T-cells; NK-cells (natural killer); Myeloid cells; Plasma CD138+ cells (BM only).

Synonyms

  • FS PN XY SRT
  • SUB CELLS FISH : XY
  • FISH XY PANEL SORT
  • XY SORT PANEL
  • FISH PANEL : XY SORTED CELLS PANEL

Methodology

Lymphocyte subsets are magnetically isolated using positive cell selection.  Cell types are then analyzed separately using fluorescence in situ hybridization (FISH).

Reported

1-2 days

CPT Codes

For Cell Separation Procedure:
88184, 88185(x6)

For FISH Testing:
88237, 88271(x2), 88275
Collection

Collect

Blood or Bone Marrow in 5-3mL Lav EDTA. MUST schedule with Genetics (513) 636-4474 before collection on only Mon-Fri. Must be received to Burnet lab by 1400 if collected on Fri.


Minimum Collection Volume

Blood:  12 mL 
Bone Marrow: 3 mL 

The laboratory cannot assure a result if the minimum volumes are not met.  Contact GENETICS for more information on minimum volumes.

Acceptable Specimen Collect Alternatives

The laboratory can obtain results for this test using different collection tube types. For best results, send one of the collection tube type listed above.  If only an alternate collection tube type is available, please call the laboratory at (513) 636-4474 for more information on how to obtain and/or handle any alternate tube types and whether or not they are acceptable for this testing.

Shipping/Handling Instructions (Lab Use Only)

Room Temperature. Do NOT spin. Burnet: Call GENETICS (6-4474) immediately upon arrival.

External Client Shipping and Handling

Store at room temperature  / Use overnight shipping (protect from temperature extremes, no ice) or call the lab for local courier service (contact laboratory prior to drawing specimen to ensure courier area coverage).  Do NOT spin tubes.

Stability (from collection to initiation)

Room Temp 72 hrs. 

Notes

The cell sorting MUST be scheduled with the CCHMC GENETICS Laboratory ahead of time. Please call:  (513)636-4474  to schedule. Collection personnel MUST initial specimen container to confirm sample identity.

Performing Lab

Cytogenetics Laboratory 
Phone: (513) 636-4474
Fax: (513) 636-4414

Hours: Dayshift (Monday through Friday)
Ordering

Ordering Recommendations

Please indicate what types of cells require separation and list the PRIORITY of cell types wanted, in case there is not enough sample to perform separation for all cell types listed. Possible Cell Types:  B-cells; T-cells; NK-cells (natural killer); Myeloid cells; Plasma CD138+ cells (BM only).

Synonyms

  • FS PN XY SRT
  • SUB CELLS FISH : XY
  • FISH XY PANEL SORT
  • XY SORT PANEL
  • FISH PANEL : XY SORTED CELLS PANEL

Methodology

Lymphocyte subsets are magnetically isolated using positive cell selection.  Cell types are then analyzed separately using fluorescence in situ hybridization (FISH).

Reported

1-2 days
Result Interpretation
Laboratory Personnel Use

CPT Codes

For Cell Separation Procedure:
88184, 88185(x6)

For FISH Testing:
88237, 88271(x2), 88275