Ordering Guidelines

Please include completed Consent Form with order

Test Tube

PREFERED:  

ALTERNATE: Green Top Tube or Red Top Tube
 


 

Collect

Serum separator tube.

Specimen Preparation

Transfer 1 mL serum  to an ARUP Standard Transport Tube. (Min: 0.3 mL)

Unacceptable Conditions

EDTA plasma, tissue or urine. Grossly hemolyzed or lipemic specimens.

Storage/Transport Temperature

Please draw blood and send to central lab

Stability (from collection to initiation)

After separation from cells: Ambient: 72 hours; Refrigerated: 1 week; Frozen: 6 months

Referral Lab

Ordering Recommendations

Preferred test to diagnose vitamin D insufficiency and monitor response to therapy. Testing is recommended only for patients at risk for vitamin D insufficiency.

Ordering Guidelines

Please include completed Consent Form with order

Performed

Sun-Sat

Reported

Within 24 hours

Methodology

Quantitative Chemiluminescent Immunoassay

Synonyms

  • Vit D
  • D, 25-Hydroxy, Vitamin
  • vitamin D
  • Ergocalciferol

Storage/Transport Temperature

Please draw blood and send to central lab

Reference Interval

0-17 years
DeficiencyLess than 20 ng/mL
Optimum levelGreater than or equal to 20 ng/mL*
*(Wagner CL et al. Pediatrics 2008; 122: 1142-52.)

18 years and older
DeficiencyLess than 20 ng/mL
Insufficiency20-29 ng/mL
Optimum Level30-80 ng/mL
Possible ToxicityGreater than 150 ng/mL

Interpretive Data

This assay accurately quantifies the sum of vitamin D3, 25-Hydroxy and vitamin D2, 25-Hydroxy. 

Test Tube

PREFERED:  

ALTERNATE: Green Top Tube or Red Top Tube
 


 

CPT Codes

82306

LOINC

  • 1989-3

Performing Lab

ARUP Laboratories
Collection

Ordering Guidelines

Please include completed Consent Form with order

Test Tube

PREFERED:  

ALTERNATE: Green Top Tube or Red Top Tube
 


 

Collect

Serum separator tube.

Specimen Preparation

Transfer 1 mL serum  to an ARUP Standard Transport Tube. (Min: 0.3 mL)

Unacceptable Conditions

EDTA plasma, tissue or urine. Grossly hemolyzed or lipemic specimens.

Storage/Transport Temperature

Please draw blood and send to central lab

Stability (from collection to initiation)

After separation from cells: Ambient: 72 hours; Refrigerated: 1 week; Frozen: 6 months

Referral Lab

Ordering

Ordering Recommendations

Preferred test to diagnose vitamin D insufficiency and monitor response to therapy. Testing is recommended only for patients at risk for vitamin D insufficiency.

Ordering Guidelines

Please include completed Consent Form with order

Performed

Sun-Sat

Reported

Within 24 hours

Methodology

Quantitative Chemiluminescent Immunoassay

Synonyms

  • Vit D
  • D, 25-Hydroxy, Vitamin
  • vitamin D
  • Ergocalciferol

Storage/Transport Temperature

Please draw blood and send to central lab

Result Interpretation

Reference Interval

0-17 years
DeficiencyLess than 20 ng/mL
Optimum levelGreater than or equal to 20 ng/mL*
*(Wagner CL et al. Pediatrics 2008; 122: 1142-52.)

18 years and older
DeficiencyLess than 20 ng/mL
Insufficiency20-29 ng/mL
Optimum Level30-80 ng/mL
Possible ToxicityGreater than 150 ng/mL

Interpretive Data

This assay accurately quantifies the sum of vitamin D3, 25-Hydroxy and vitamin D2, 25-Hydroxy. 

Test Tube

PREFERED:  

ALTERNATE: Green Top Tube or Red Top Tube
 


 

Administrative

CPT Codes

82306

LOINC

  • 1989-3

Performing Lab

ARUP Laboratories