Minimum requirements: One 3 mL plain red top tube (blood).
Unacceptable Conditions
Hemolyzed samples are unacceptable for testing.
Storage/Transport Temperature
Store sample at room temperature and transport to the lab for testing.
Stability (from collection to initiation)
Specimen is stable at room temperature.
Performed
Testing performed by Emory Medical Labs- HLA lab.
Routine: Mon-Fri 24 Hr.
STAT: Mon-Fri 24 Hr. Weekends on call.
Remarks
Do not spin and/or pour of the sample. Do not freeze sample.
Ordering Recommendations
This order is specifically for monitoring organ recipients post transplant for donor specific antibodies.
Performed
Testing performed by Emory Medical Labs- HLA lab.
Routine: Mon-Fri 24 Hr.
STAT: Mon-Fri 24 Hr. Weekends on call.
Methodology
Flow Cytometry.
Reported
Routine: 14 days
STAT: 2 days
Notes
Original Tubes Preferred
Section
HLA
Group Components
HLA Antibody Screen
HLA Class I Antibody Specificity
HLA Class II Antibody Specificity
HLA Donor Specific Antibody
Medicare Regulated Tests
No
Reference Interval
N/A
Interpretive Data
HLA Antibody Screen- 3-100% considered Positive (architecture consistent with antibody presence)
0-2% considered Negative
HLA Class I Antibody Specificity- HLA -A, -B, -Bw, -C: MFI (Median Fluorescence Intensity) range: 0 – 400000.
MFI values >500 may be considered Positive. Final interpretation made by laboratory Director/Supervisor.
HLA Class II Antibody Specificity- HLA-DRB1, -DQA1, -DQB1, -DRB3, -DRB4, -DRB5, -DPA1, -DPB1: MFI (Median Fluorescence Intensity) range: 0 – 400000.
MFI values >500 may be considered Positive. Final interpretation made by laboratory Director/Supervisor.
HLA Post Transplant Monitoring DSA- Positive/Negative for Donor Specific Antibody (Dependent upon specified Donor HLA Typing)
CPT Codes
HLA Antibody Screen 86828
HLA Class I Antibody Specificity 86832
HLA Class II Antibody Specificity 86833
HLA Donor Specific Antibody N/A
Collection
Collect
One 10 mL plain red top (blood)
Specimen Preparation
Transport 10.0 ml plain red top(blood). Min:(3ml)
Pediatric Collection
Two 3mL OR One 10mL plain red top tube (blood).
Minimum requirements: One 3 mL plain red top tube (blood).
Unacceptable Conditions
Hemolyzed samples are unacceptable for testing.
Storage/Transport Temperature
Store sample at room temperature and transport to the lab for testing.
Stability (from collection to initiation)
Specimen is stable at room temperature.
Performed
Testing performed by Emory Medical Labs- HLA lab.
Routine: Mon-Fri 24 Hr.
STAT: Mon-Fri 24 Hr. Weekends on call.
Remarks
Do not spin and/or pour of the sample. Do not freeze sample.
Ordering
Ordering Recommendations
This order is specifically for monitoring organ recipients post transplant for donor specific antibodies.
Performed
Testing performed by Emory Medical Labs- HLA lab.
Routine: Mon-Fri 24 Hr.
STAT: Mon-Fri 24 Hr. Weekends on call.
Methodology
Flow Cytometry.
Reported
Routine: 14 days
STAT: 2 days
Notes
Original Tubes Preferred
Section
HLA
Group Components
HLA Antibody Screen
HLA Class I Antibody Specificity
HLA Class II Antibody Specificity
HLA Donor Specific Antibody
Medicare Regulated Tests
No
Result Interpretation
Reference Interval
N/A
Interpretive Data
HLA Antibody Screen- 3-100% considered Positive (architecture consistent with antibody presence)
0-2% considered Negative
HLA Class I Antibody Specificity- HLA -A, -B, -Bw, -C: MFI (Median Fluorescence Intensity) range: 0 – 400000.
MFI values >500 may be considered Positive. Final interpretation made by laboratory Director/Supervisor.
HLA Class II Antibody Specificity- HLA-DRB1, -DQA1, -DQB1, -DRB3, -DRB4, -DRB5, -DPA1, -DPB1: MFI (Median Fluorescence Intensity) range: 0 – 400000.
MFI values >500 may be considered Positive. Final interpretation made by laboratory Director/Supervisor.
HLA Post Transplant Monitoring DSA- Positive/Negative for Donor Specific Antibody (Dependent upon specified Donor HLA Typing)
Administrative
CPT Codes
HLA Antibody Screen 86828
HLA Class I Antibody Specificity 86832
HLA Class II Antibody Specificity 86833
HLA Donor Specific Antibody N/A
