Allow Gold tube to sit for 30 minutes to form clot. Spin for 10 minutes at 1100-1300 g in a swing bucket unit or 15 minutes in a fixed angle to separate cells from serum. Remember to properly label tube with patient name, DOB, test and collect date. Do not collect in orange top tube. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Minimum 0.5 mL) . Separate serum from cells ASAP or within 2 hours of collection.
Transfer 1.2 mL serum to an ARUP Standard Transport Tube. (Min: 0.5 mL)
Storage/Transport Temperature
Frozen
Stability (from collection to initiation)
After separation from cells: Ambient: 24 hours; Refrigerated: 1 week; Frozen: 3 months
Ordering Recommendations
Most useful to establish autoimmune etiology in previously diagnosed type 1 diabetes mellitus (DM). Do not use to differentiate type 1 DM from type 2 DM, for most cases. If pursuing antibody testing to determine autoimmune DM, perform at least two antibody tests. In most cases, use glutamic acid decarboxylase antibody in combination with another antibody test. See ARUP site for more details. http://ltd.aruplab.com/Tests/Pub/2001771
A value greater than 5.0 IU/mL is considered positive for Glutamic Acid Decarboxylase Antibody (GAD Ab). This assay is intended for the semi-quantitative determination of the GAD Ab in human serum. Results should be interpreted within the context of clinical symptoms Interpretation and Reference Range
CPT Codes
86341
LOINC Codes
NAME
LOINC
Order
GAD65 Antibody
56540-8
Test Code (Outreach Synonym)
10420
Catalog Code
6542480063
Collection
Collect
Blood, Gold
Preferred Draw Volume
3 mL
Minimum Draw Volume
2 mL
Unacceptable Conditions
Plasma. Grossly hemolyzed
Collection Comments
Allow Gold tube to sit for 30 minutes to form clot. Spin for 10 minutes at 1100-1300 g in a swing bucket unit or 15 minutes in a fixed angle to separate cells from serum. Remember to properly label tube with patient name, DOB, test and collect date. Do not collect in orange top tube. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Minimum 0.5 mL) . Separate serum from cells ASAP or within 2 hours of collection.
Transfer 1.2 mL serum to an ARUP Standard Transport Tube. (Min: 0.5 mL)
Storage/Transport Temperature
Frozen
Stability (from collection to initiation)
After separation from cells: Ambient: 24 hours; Refrigerated: 1 week; Frozen: 3 months
Ordering
Ordering Recommendations
Most useful to establish autoimmune etiology in previously diagnosed type 1 diabetes mellitus (DM). Do not use to differentiate type 1 DM from type 2 DM, for most cases. If pursuing antibody testing to determine autoimmune DM, perform at least two antibody tests. In most cases, use glutamic acid decarboxylase antibody in combination with another antibody test. See ARUP site for more details. http://ltd.aruplab.com/Tests/Pub/2001771
A value greater than 5.0 IU/mL is considered positive for Glutamic Acid Decarboxylase Antibody (GAD Ab). This assay is intended for the semi-quantitative determination of the GAD Ab in human serum. Results should be interpreted within the context of clinical symptoms Interpretation and Reference Range
