Whole blood or plasma on ice. Clotted sample. Sample collected in glass tube. Unspun specimen > 4 hrs old. Underfilled tube. Gross hemolysis.
Collection Comments
Full, 2.7mL Blue, BD 3.2% Na Citrate plastic tube. Do not send whole blood or plasma to lab on ice. Double spin and freeze plasma within 4 hrs of collection: Spin at 3000rpm for 15 min. Place top 2/3 of plasma in aliquot tube and spin aliquot at 3000 rpm for 15 min. Take top 2/3 of 2nd spin and put in another aliquot tube. Mark tube ‘plasma'. Freeze immediately. Send frozen platelet poor plasma. Remember to properly label the aliquot tube with patient name, DOB, test and collect date.
Factor XII(12) Assay is useful to detect specific coagulaton factor activity. Deficiencies of factors IX, XI, and XII can be seen in some cases of liver disease, though these decreases may not be significant. Factor levels may also be decreased in somecases of disseminated intravascular coagulation due to mass consumption of factors.
Whole blood or plasma on ice. Clotted sample. Sample collected in glass tube. Unspun specimen > 4 hrs old. Underfilled tube. Gross hemolysis.
Collection Comments
Full, 2.7mL Blue, BD 3.2% Na Citrate plastic tube. Do not send whole blood or plasma to lab on ice. Double spin and freeze plasma within 4 hrs of collection: Spin at 3000rpm for 15 min. Place top 2/3 of plasma in aliquot tube and spin aliquot at 3000 rpm for 15 min. Take top 2/3 of 2nd spin and put in another aliquot tube. Mark tube ‘plasma'. Freeze immediately. Send frozen platelet poor plasma. Remember to properly label the aliquot tube with patient name, DOB, test and collect date.
Factor XII(12) Assay is useful to detect specific coagulaton factor activity. Deficiencies of factors IX, XI, and XII can be seen in some cases of liver disease, though these decreases may not be significant. Factor levels may also be decreased in somecases of disseminated intravascular coagulation due to mass consumption of factors.
