Plasma not separated within 6 hours of collection
Whole Blood frozen
Specimen collected in heparin
Specimen contains less than 1 mL of plasma
Shared specimens that have been tested on the automation line in Chemistry or Hematology
Collection Comments
Invert tube 8-10 times to mix blood with anticoagulant. Centrifuge blood for 10 minutes at 1600 g in a swing-bucket unit or 15 minutes for a fixed angle unit. Process within 6 hours of collection. Aliquot plasma into plastic tube and refrigerate. Plasma is stable for 6 days refrigerated, if longer than 6 days, plasma must be frozen.
Storage/Transport Temperature
Refrigerated
Stability (from collection to initiation)
Room Temperature: not recommended
Refrigerated: 6 days
Frozen: 84 days
Special Handling
Spin within 6 hours, Refrigerate
Ordering Recommendations
Human Immunodeficiency Virus (HIV) is the etiologic agent of Acquired Immunodeficiency Syndrome (AIDS). HIV-1 viral load is used to aid in monitoring the disease progress for clinical management of HIV-1 infected patients. This test is not intended for the use of screening for the presence of HIV-1 or as a diagnostic test for HIV-1 infection. Primers and probes are specific for HIV-1 group M. The performance of this assay has not been evaluated with specimens containing HIV-1 group O or N, nor with specimens containing HIV-2.
Performed
Monday - Friday; Days
Methodology
RT-PCR
Reported
2-3 days
Performing Lab
Molecular
Synonyms
94600
HIV 1 RNA Standard QN
Standard HIV 1 RNA QN
HIV-1 Quantitative PCR Test (Viral Load)
HIV RNA Viral Load PCR QN
Reference Interval
20-1.00 E7 copies/mL
Assay Limitation(s)
Interference: Heparin
CPT Codes
87536
LOINC Codes
NAME
LOINC
Order
HIV RNA Viral Load
20447-9
Result
HIV RNA
24013-5
HIV RNA Viral Load
20447-9
Log10 HIV-1 RNA QN
29541-0
Test Code (Outreach Synonym)
94600
Catalog Code
693586
Collection
Collect
Blood, Lavender
Preferred Draw Volume
5 mL
Minimum Draw Volume
Whole Blood: 2 mL
Plasma: 1 mL
Other Acceptable Containers
Pearl
Unacceptable Conditions
Plasma not separated within 6 hours of collection
Whole Blood frozen
Specimen collected in heparin
Specimen contains less than 1 mL of plasma
Shared specimens that have been tested on the automation line in Chemistry or Hematology
Collection Comments
Invert tube 8-10 times to mix blood with anticoagulant. Centrifuge blood for 10 minutes at 1600 g in a swing-bucket unit or 15 minutes for a fixed angle unit. Process within 6 hours of collection. Aliquot plasma into plastic tube and refrigerate. Plasma is stable for 6 days refrigerated, if longer than 6 days, plasma must be frozen.
Storage/Transport Temperature
Refrigerated
Stability (from collection to initiation)
Room Temperature: not recommended
Refrigerated: 6 days
Frozen: 84 days
Special Handling
Spin within 6 hours, Refrigerate
Ordering
Ordering Recommendations
Human Immunodeficiency Virus (HIV) is the etiologic agent of Acquired Immunodeficiency Syndrome (AIDS). HIV-1 viral load is used to aid in monitoring the disease progress for clinical management of HIV-1 infected patients. This test is not intended for the use of screening for the presence of HIV-1 or as a diagnostic test for HIV-1 infection. Primers and probes are specific for HIV-1 group M. The performance of this assay has not been evaluated with specimens containing HIV-1 group O or N, nor with specimens containing HIV-2.
