Allow Gold tube to sit for 30 minutes to form clot. Spin for 10 minutes at 1100-1300 g in a swing bucket unit or 15 minutes in a fixed angle to separate cells from serum. Send spun tube room temp. Refrigerated is acceptable. Remember to properly label tube with patient name, DOB, test and collect date. Indicate 'serum' or 'plasma' on label.
HIV-1/-2 Ab/HIV Ag QL test is a qualitative test for the presence of antibodies to human immunodeficiency virus 1 and human immunodeficiency virus 2. Professional organizations have different recommendations for HIV antibody testing, however, testing for HIV antibody is ordered for patients in high risk group or individuals with a history of possible exposure to HIV 1 or 2. This test also detects the HIV 1 Ag as well as the HIV 1 and 2 Ab. Positives reflex to HIV 1/2 Ab Confirmation as appropriate.
Performed
Monday - Friday;Days, Evenings, Nights
Methodology
Chemiluminescent Microparticle Immunoassay (CMIA)
Reported
2-3 days
Performing Lab
Chemistry
Synonyms
4500
HIV 1/2 Ab HIV Ag QL
HIV 1/HIV 2 Ab HIV Ag QL
HIV-1/-2 Ab/HIV Ag QL GP
HIVAgAb
HIV -1/-2 Ab
HIV Screen
HIV 4th Generation
p24 Antigen
Reference Interval
Negative or Non-reactive
Clinical Interpretation
HIV Algorithm Recommendations: HIVAgAb: If negative or nonreactive, no further testing needed.
If positive or reactive, reflex to HIV ½ Ab Confirmation. HIVCONFM: If HIV AB Negative, or Indeterminate (HIV-1 Indtrmnt, HIV-2 Indtrmnt, HIV Indtrmnt) further testing is needed. Followup with HIV-1 RNA, Qualitative, TMA testing. Send EDTA (lavender) tube for sendout testing for HIV1TMA.
If HIV-1 Positive, the sample is positive for HIV 1 antibodies. If HIV 1 Pos*, the sample is positive for HIV 1 antibodies and indeterminate for HIV 2 antibodies. Further testing may be recommended if clinically necessary.
If HIV-2 Positive, the sample is positive for HIV 2 antibodies. If HIV 2 Pos*, the sample is positive for HIV 2 antibodies and indeterminate for HIV 1 antibodies. Further testing may be recommended if clinically necessary.
If HIV-2 + wHIV-1, the sample is positive for HIV-2 antibodies with HIV-1 cross-reactivity. Further testing may be recommended if clinically necessary.
If HIV POS Untype, the sample is positive for HIV antibodies, but is untypable. Further confirmatory HIV-1 and HIV-2 nucleic acid testing (NAT) is recommended. HIV1TMA: If positive, it confirms HIV-1 infection in persons who may have an acute HIV-1 infection or repeatedly test positive for HIV-1 infection using the HIVAgAb.
If negative, it helps to resolve indeterminate or inconclusive HIV results. Further or repeat testing may be needed if suspicion is still present.
CPT Codes
87389; reflex 86701 and 86702
LOINC Codes
NAME
LOINC
Order
HIV-1/-2 Antibody/HIV Antigen QL
56888-1
Result
HIV-1/-2 Ab/HIV Ag QL
56888-1
HIVAgAb Interp
13499-9
Test Code (Outreach Synonym)
4500
Catalog Code
768938821
Collection
Test Information
This assay qualitatively detects HIV-1 p24 antigen and antibodies to HIV-1 (group M and group O) and HIV-2 in human serum or plasma.
Allow Gold tube to sit for 30 minutes to form clot. Spin for 10 minutes at 1100-1300 g in a swing bucket unit or 15 minutes in a fixed angle to separate cells from serum. Send spun tube room temp. Refrigerated is acceptable. Remember to properly label tube with patient name, DOB, test and collect date. Indicate 'serum' or 'plasma' on label.
HIV-1/-2 Ab/HIV Ag QL test is a qualitative test for the presence of antibodies to human immunodeficiency virus 1 and human immunodeficiency virus 2. Professional organizations have different recommendations for HIV antibody testing, however, testing for HIV antibody is ordered for patients in high risk group or individuals with a history of possible exposure to HIV 1 or 2. This test also detects the HIV 1 Ag as well as the HIV 1 and 2 Ab. Positives reflex to HIV 1/2 Ab Confirmation as appropriate.
Performed
Monday - Friday;Days, Evenings, Nights
Methodology
Chemiluminescent Microparticle Immunoassay (CMIA)
Reported
2-3 days
Performing Lab
Chemistry
Synonyms
4500
HIV 1/2 Ab HIV Ag QL
HIV 1/HIV 2 Ab HIV Ag QL
HIV-1/-2 Ab/HIV Ag QL GP
HIVAgAb
HIV -1/-2 Ab
HIV Screen
HIV 4th Generation
p24 Antigen
Result Interpretation
Reference Interval
Negative or Non-reactive
Clinical Interpretation
HIV Algorithm Recommendations: HIVAgAb: If negative or nonreactive, no further testing needed.
If positive or reactive, reflex to HIV ½ Ab Confirmation. HIVCONFM: If HIV AB Negative, or Indeterminate (HIV-1 Indtrmnt, HIV-2 Indtrmnt, HIV Indtrmnt) further testing is needed. Followup with HIV-1 RNA, Qualitative, TMA testing. Send EDTA (lavender) tube for sendout testing for HIV1TMA.
If HIV-1 Positive, the sample is positive for HIV 1 antibodies. If HIV 1 Pos*, the sample is positive for HIV 1 antibodies and indeterminate for HIV 2 antibodies. Further testing may be recommended if clinically necessary.
If HIV-2 Positive, the sample is positive for HIV 2 antibodies. If HIV 2 Pos*, the sample is positive for HIV 2 antibodies and indeterminate for HIV 1 antibodies. Further testing may be recommended if clinically necessary.
If HIV-2 + wHIV-1, the sample is positive for HIV-2 antibodies with HIV-1 cross-reactivity. Further testing may be recommended if clinically necessary.
If HIV POS Untype, the sample is positive for HIV antibodies, but is untypable. Further confirmatory HIV-1 and HIV-2 nucleic acid testing (NAT) is recommended. HIV1TMA: If positive, it confirms HIV-1 infection in persons who may have an acute HIV-1 infection or repeatedly test positive for HIV-1 infection using the HIVAgAb.
If negative, it helps to resolve indeterminate or inconclusive HIV results. Further or repeat testing may be needed if suspicion is still present.
