Body fluid, Biopsy, Tissue, Wound, Other; anaerobic transport medium (ATM) or syringe (needle removed and capped)
Biopsy/Tissue ONLY; leak-proof sterile container
**Gastric or Duodenal Tissue/Biopsy for Helicobacter pylori - see collection comments. If submitting tissue/biopsy for H. pylori, please notify Microbiology Laboratory in advance (317) 491-6999.
Preferred Draw Volume
1 mL or 3mm piece of gastric or duodenal tissue must be submitted for H. pylori testing.
Unacceptable Conditions
Red capped Stuart's swab is NOT acceptable for anaerobe culture.
Collection Comments
Must be received in 24 hours. Indicate site, patient age, current antibiotic therapy, and clinical diagnosis. Gastric or Duodenal Tissue/Biopsy for Helicobacter pylori—order as Anaerobe CX and include specimen source and “test for H. pylori” in the comments/on the requisition. If susceptibilities are needed, please note “Send out isolates for susceptibilities” in the comments/on the requisition. If submitted in leak-proof sterile container or Stuart's storage media, must be received in lab within 3 hours of collection at ambient temperature.
If submitted in Brucella broth (20% glycerol, cysteine-albumin broth) or liquid Cary-Blair medium, must be received within 24 hours of collection at ambient temperature.
Storage/Transport Temperature
Room Temperature
Gastric or Duodenal Tissue/Biopsy for Helicobacter pylori (H. pylori) testing: Submit to Microbiology ASAP according to information listed in collection comments.
Ordering Recommendations
An Anaerobe CX+Stn (culture and stain) includes both a culture for anaerobic bacteria and a microscopic examination of a Gram-stained smear. An abscess specimen is a collection of pus, which is typically encapsulated, that is formed by an infectious process. Anaerobic bacteria can cause infections when they have access to normally-sterile body fluids, such as pleural and abdominal fluids. A "drain" is a tube a caregiver places to remove pus, blood, or other fluids from a wound. Historically, anaerobic bacteria have been isolated from tissue specimens taken from many different body sites, especially abdominal, thoracic, and central nervous system locations. Anerobic bacteria are frequent causes of wound infections, especially foreign-body and surgical wounds. All anaerobic bacteria that grow in the culture are definitively identified, but antibiotic susceptibility testing (AST) is not routinely performed. Rather, AST is done by request only. This test is ordered when there is a suspicion that the patient may have an infection caused by anerobic bacteria.
Performed
Monday - Sunday; Days
Methodology
Culture, Gram Stain & Identification
Reported
5-10 days
Performing Lab
Microbiology
Synonyms
3050
Anaerobe Culture
Anaerobic Culture + Stain
Culture Anaerobe
Anaerobeic Culture and Gram Stain
Anaerobe CX and stain
Reference Interval
No anaerobic growth
CPT Codes
87075
LOINC Codes
NAME
LOINC
Order
Anaerobic Culture + Stain
635-3
Test Code (Outreach Synonym)
3050
Catalog Code
692445
Collection
Collect
Body fluid, Biopsy, Tissue, Wound, Other; anaerobic transport medium (ATM) or syringe (needle removed and capped)
Biopsy/Tissue ONLY; leak-proof sterile container
**Gastric or Duodenal Tissue/Biopsy for Helicobacter pylori - see collection comments. If submitting tissue/biopsy for H. pylori, please notify Microbiology Laboratory in advance (317) 491-6999.
Preferred Draw Volume
1 mL or 3mm piece of gastric or duodenal tissue must be submitted for H. pylori testing.
Unacceptable Conditions
Red capped Stuart's swab is NOT acceptable for anaerobe culture.
Collection Comments
Must be received in 24 hours. Indicate site, patient age, current antibiotic therapy, and clinical diagnosis. Gastric or Duodenal Tissue/Biopsy for Helicobacter pylori—order as Anaerobe CX and include specimen source and “test for H. pylori” in the comments/on the requisition. If susceptibilities are needed, please note “Send out isolates for susceptibilities” in the comments/on the requisition. If submitted in leak-proof sterile container or Stuart's storage media, must be received in lab within 3 hours of collection at ambient temperature.
If submitted in Brucella broth (20% glycerol, cysteine-albumin broth) or liquid Cary-Blair medium, must be received within 24 hours of collection at ambient temperature.
Storage/Transport Temperature
Room Temperature
Gastric or Duodenal Tissue/Biopsy for Helicobacter pylori (H. pylori) testing: Submit to Microbiology ASAP according to information listed in collection comments.
Ordering
Ordering Recommendations
An Anaerobe CX+Stn (culture and stain) includes both a culture for anaerobic bacteria and a microscopic examination of a Gram-stained smear. An abscess specimen is a collection of pus, which is typically encapsulated, that is formed by an infectious process. Anaerobic bacteria can cause infections when they have access to normally-sterile body fluids, such as pleural and abdominal fluids. A "drain" is a tube a caregiver places to remove pus, blood, or other fluids from a wound. Historically, anaerobic bacteria have been isolated from tissue specimens taken from many different body sites, especially abdominal, thoracic, and central nervous system locations. Anerobic bacteria are frequent causes of wound infections, especially foreign-body and surgical wounds. All anaerobic bacteria that grow in the culture are definitively identified, but antibiotic susceptibility testing (AST) is not routinely performed. Rather, AST is done by request only. This test is ordered when there is a suspicion that the patient may have an infection caused by anerobic bacteria.
