For Serum collect Gold
For Plasma collect Lavender
Preferred Draw Volume
4 mL
Minimum Draw Volume
3 mL
Other Acceptable Containers
Red
Unacceptable Conditions
>14 days old
Collection Comments
Allow Gold tube to sit for 30 minutes to form clot. Spin for 10 minutes at 1100-1300 g in a swing bucket unit or 15 minutes in a fixed angle to separate cells from serum. Remember to properly label tube with patient name, DOB, test and collect date. Do not collect in orange top tube. Transfer 1.2 mL serum/plasma to an aliquot tube. (Minimum 1.2 mL) . When multiple tests are ordered on a sample type, please submit combined volume per test. https://miravistalabs.com/medical-fungal-infection-testing/antibody-detection/histoplasma-antibody-igg-igm-eia/
Storage/Transport Temperature
Frozen
Stability (from collection to initiation)
After separation from cells: Ambient: 14 days; Refrigerated: 14 days; Frozen: 14 days
Ordering Recommendations
IgM and IgG antibodies to Histoplasma antigen usually appear during the first month of infection. The IgM antibody response in acute pulmonary histoplasmosis is detectable during the acute phase (roughly 3 weeks) and is shown to decline during the convalescent stage (at about 6 weeks); whereas, IgG levels remained relatively constant at 6 weeks. Follow-up testing may be Follow-up testing may be considered 2-4 weeks after initial testing to determine if antibody levels are increasing, especially in patients with low positive (10 EU - 20 EU) or intermediate results. https://miravistalabs.com/medical-fungal-infection-testing/antibody-detection/histoplasma-antibody-igg-igm-eia/
For Serum collect Gold
For Plasma collect Lavender
Preferred Draw Volume
4 mL
Minimum Draw Volume
3 mL
Other Acceptable Containers
Red
Unacceptable Conditions
>14 days old
Collection Comments
Allow Gold tube to sit for 30 minutes to form clot. Spin for 10 minutes at 1100-1300 g in a swing bucket unit or 15 minutes in a fixed angle to separate cells from serum. Remember to properly label tube with patient name, DOB, test and collect date. Do not collect in orange top tube. Transfer 1.2 mL serum/plasma to an aliquot tube. (Minimum 1.2 mL) . When multiple tests are ordered on a sample type, please submit combined volume per test. https://miravistalabs.com/medical-fungal-infection-testing/antibody-detection/histoplasma-antibody-igg-igm-eia/
Storage/Transport Temperature
Frozen
Stability (from collection to initiation)
After separation from cells: Ambient: 14 days; Refrigerated: 14 days; Frozen: 14 days
Ordering
Ordering Recommendations
IgM and IgG antibodies to Histoplasma antigen usually appear during the first month of infection. The IgM antibody response in acute pulmonary histoplasmosis is detectable during the acute phase (roughly 3 weeks) and is shown to decline during the convalescent stage (at about 6 weeks); whereas, IgG levels remained relatively constant at 6 weeks. Follow-up testing may be Follow-up testing may be considered 2-4 weeks after initial testing to determine if antibody levels are increasing, especially in patients with low positive (10 EU - 20 EU) or intermediate results. https://miravistalabs.com/medical-fungal-infection-testing/antibody-detection/histoplasma-antibody-igg-igm-eia/