Collect

Specimen Type Type of Container Volume of Specimen Status
Throat swab Viral Transport Media   Preferred
Genital swab Viral Transport Media   Preferred
Rectal swab Viral Transport Media   Preferred
Skin/Lesion scraping Viral Transport Media   Preferred
Ocular specimen Viral Transport Media   Preferred
Tracheal aspirate Sterile container 1 mL Preferred
BAL Sterile container 1 mL Preferred
Respiratory tract specimen Sterile container 1 mL Preferred

Outpatient Specimen Preparation

Throat swab, Genital specimen, Rectal swab, Skin/Lesion scraping, Ocular specimen, Tracheal aspirate, BAL, Respiratory tract specimen:
      Transport to laboratory as soon as possible
      If delay in transport greater than 1 hour, refrigerate
 

Unacceptable Conditions

Swabs transported without the use of Viral Transport Media, Diluted specimen, Thawed and refrozen specimen, Not received at proper temperature, Not received within specified time frame

Stability

Throat swab, Genital specimen, Rectal swab, Skin/Lesion scraping, Ocular specimen, Tracheal aspirate, BAL, Respiratory tract specimen: Room temperature 1 hour(s)

Throat swab, Genital specimen, Rectal swab, Skin/Lesion scraping, Ocular specimen:, Tracheal aspirate, BAL, Respiratory tract specimen: Refrigerated 4 day(s)

Throat swab, Genital specimen, Rectal swab, Skin/Lesion scraping, Ocular specimen:, Tracheal aspirate, BAL, Respiratory tract specimen: Frozen Indefinite week(s)
 

Remarks

Lesion specimens should contain cells. Break vesicle and scrape lesion to obtain adequate specimen. Swab specimens must be transported in Viral Transport Media.Viral Transport Media vials received without the swab may yield reduced sensitivity.

HSVTT2 detects both HSV-1 and HSV-2 and positive results are reported along with the type.

Days Performed

Daily

Set Up Schedule

Dayshift

Typical Turnaround

2 days

Lab Area

CMI, Molecular Infectious Disease

Methodology

Real-time Polymerase Chain Reaction (PCR)

CPT Codes

87529

Synonyms

  • Herpes Simplex Virus by PCR, Superficial Site, HSV by PCR Superfical Site, HSV by PCR Superficial Qualitative
  • HSV qual
  • HSV qualitative

Collect

Specimen Type Type of Container Volume of Specimen Status
Throat swab Viral Transport Media   Preferred
Genital swab Viral Transport Media   Preferred
Rectal swab Viral Transport Media   Preferred
Skin/Lesion scraping Viral Transport Media   Preferred
Ocular specimen Viral Transport Media   Preferred
Tracheal aspirate Sterile container 1 mL Preferred
BAL Sterile container 1 mL Preferred
Respiratory tract specimen Sterile container 1 mL Preferred

Minimum Volume

Specimen Type Type of Container Minimum Volume
Throat swab, Genital swab, Rectal swab, Skin/Lesion scraping, Ocular specimen Viral Transport Media  
Tracheal aspirate, BAL, Respiratory tract specimen Sterile container 0.4 mL

Inpatient Specimen Preparation

Throat swab, Genital specimen, Rectal swab, Skin/Lesion scraping, Ocular specimen, Tracheal aspirate, BAL, Respiratory tract specimen:
      Transport to laboratory as soon as possible

Unacceptable Conditions

Swabs transported without the use of Viral Transport Media, Diluted specimen, Thawed and refrozen specimen, Not received at proper temperature, Not received within specified time frame

Stability

Throat swab, Genital specimen, Rectal swab, Skin/Lesion scraping, Ocular specimen, Tracheal aspirate, BAL, Respiratory tract specimen: Room temperature 1 hour(s)

Throat swab, Genital specimen, Rectal swab, Skin/Lesion scraping, Ocular specimen:, Tracheal aspirate, BAL, Respiratory tract specimen: Refrigerated 4 day(s)

Throat swab, Genital specimen, Rectal swab, Skin/Lesion scraping, Ocular specimen:, Tracheal aspirate, BAL, Respiratory tract specimen: Frozen Indefinite week(s)
 

Remarks

Lesion specimens should contain cells. Break vesicle and scrape lesion to obtain adequate specimen. Swab specimens must be transported in Viral Transport Media.Viral Transport Media vials received without the swab may yield reduced sensitivity.

HSVTT2 detects both HSV-1 and HSV-2 and positive results are reported along with the type.

Days Performed

Daily

Set Up Schedule

Dayshift

Typical Turnaround

2 days

CPT Codes

87529

Lab Area

CMI, Molecular Infectious Disease

Synonyms

  • Herpes Simplex Virus by PCR, Superficial Site, HSV by PCR Superfical Site, HSV by PCR Superficial Qualitative
  • HSV qual
  • HSV qualitative

Synonyms

  • Herpes Simplex Virus by PCR, Superficial Site, HSV by PCR Superfical Site, HSV by PCR Superficial Qualitative
  • HSV qual
  • HSV qualitative

CPT Codes

87529

Methodology

Real-time Polymerase Chain Reaction (PCR)

Collect

Specimen Type Type of Container Volume of Specimen Status
Throat swab Viral Transport Media   Preferred
Genital swab Viral Transport Media   Preferred
Rectal swab Viral Transport Media   Preferred
Skin/Lesion scraping Viral Transport Media   Preferred
Ocular specimen Viral Transport Media   Preferred
Tracheal aspirate Sterile container 1 mL Preferred
BAL Sterile container 1 mL Preferred
Respiratory tract specimen Sterile container 1 mL Preferred

Minimum Volume

Specimen Type Type of Container Minimum Volume
Throat swab, Genital swab, Rectal swab, Skin/Lesion scraping, Ocular specimen Viral Transport Media  
Tracheal aspirate, BAL, Respiratory tract specimen Sterile container 0.4 mL

Inpatient Specimen Preparation

Throat swab, Genital specimen, Rectal swab, Skin/Lesion scraping, Ocular specimen, Tracheal aspirate, BAL, Respiratory tract specimen:
      Transport to laboratory as soon as possible

Outpatient Specimen Preparation

Throat swab, Genital specimen, Rectal swab, Skin/Lesion scraping, Ocular specimen, Tracheal aspirate, BAL, Respiratory tract specimen:
      Transport to laboratory as soon as possible
      If delay in transport greater than 1 hour, refrigerate
 

InLab Processing

Samples received frozen must remain frozen until the time of testing.

Stability

Throat swab, Genital specimen, Rectal swab, Skin/Lesion scraping, Ocular specimen, Tracheal aspirate, BAL, Respiratory tract specimen: Room temperature 1 hour(s)

Throat swab, Genital specimen, Rectal swab, Skin/Lesion scraping, Ocular specimen:, Tracheal aspirate, BAL, Respiratory tract specimen: Refrigerated 4 day(s)

Throat swab, Genital specimen, Rectal swab, Skin/Lesion scraping, Ocular specimen:, Tracheal aspirate, BAL, Respiratory tract specimen: Frozen Indefinite week(s)
 

Unacceptable Conditions

Swabs transported without the use of Viral Transport Media, Diluted specimen, Thawed and refrozen specimen, Not received at proper temperature, Not received within specified time frame

Days Performed

Daily

Set Up Schedule

Dayshift

Typical Turnaround

2 days

Remarks

Lesion specimens should contain cells. Break vesicle and scrape lesion to obtain adequate specimen. Swab specimens must be transported in Viral Transport Media.Viral Transport Media vials received without the swab may yield reduced sensitivity.

HSVTT2 detects both HSV-1 and HSV-2 and positive results are reported along with the type.

Synonyms

  • Herpes Simplex Virus by PCR, Superficial Site, HSV by PCR Superfical Site, HSV by PCR Superficial Qualitative
  • HSV qual
  • HSV qualitative

Methodology

Real-time Polymerase Chain Reaction (PCR)

CPT Codes

87529

DC Code

5404

Downtime Availability

1-Continuous availability
Outpatient Requirements

Collect

Specimen Type Type of Container Volume of Specimen Status
Throat swab Viral Transport Media   Preferred
Genital swab Viral Transport Media   Preferred
Rectal swab Viral Transport Media   Preferred
Skin/Lesion scraping Viral Transport Media   Preferred
Ocular specimen Viral Transport Media   Preferred
Tracheal aspirate Sterile container 1 mL Preferred
BAL Sterile container 1 mL Preferred
Respiratory tract specimen Sterile container 1 mL Preferred

Outpatient Specimen Preparation

Throat swab, Genital specimen, Rectal swab, Skin/Lesion scraping, Ocular specimen, Tracheal aspirate, BAL, Respiratory tract specimen:
      Transport to laboratory as soon as possible
      If delay in transport greater than 1 hour, refrigerate
 

Unacceptable Conditions

Swabs transported without the use of Viral Transport Media, Diluted specimen, Thawed and refrozen specimen, Not received at proper temperature, Not received within specified time frame

Stability

Throat swab, Genital specimen, Rectal swab, Skin/Lesion scraping, Ocular specimen, Tracheal aspirate, BAL, Respiratory tract specimen: Room temperature 1 hour(s)

Throat swab, Genital specimen, Rectal swab, Skin/Lesion scraping, Ocular specimen:, Tracheal aspirate, BAL, Respiratory tract specimen: Refrigerated 4 day(s)

Throat swab, Genital specimen, Rectal swab, Skin/Lesion scraping, Ocular specimen:, Tracheal aspirate, BAL, Respiratory tract specimen: Frozen Indefinite week(s)
 

Remarks

Lesion specimens should contain cells. Break vesicle and scrape lesion to obtain adequate specimen. Swab specimens must be transported in Viral Transport Media.Viral Transport Media vials received without the swab may yield reduced sensitivity.

HSVTT2 detects both HSV-1 and HSV-2 and positive results are reported along with the type.

Days Performed

Daily

Set Up Schedule

Dayshift

Typical Turnaround

2 days

Lab Area

CMI, Molecular Infectious Disease

Methodology

Real-time Polymerase Chain Reaction (PCR)

CPT Codes

87529

Synonyms

  • Herpes Simplex Virus by PCR, Superficial Site, HSV by PCR Superfical Site, HSV by PCR Superficial Qualitative
  • HSV qual
  • HSV qualitative
Inpatient Requirements

Collect

Specimen Type Type of Container Volume of Specimen Status
Throat swab Viral Transport Media   Preferred
Genital swab Viral Transport Media   Preferred
Rectal swab Viral Transport Media   Preferred
Skin/Lesion scraping Viral Transport Media   Preferred
Ocular specimen Viral Transport Media   Preferred
Tracheal aspirate Sterile container 1 mL Preferred
BAL Sterile container 1 mL Preferred
Respiratory tract specimen Sterile container 1 mL Preferred

Minimum Volume

Specimen Type Type of Container Minimum Volume
Throat swab, Genital swab, Rectal swab, Skin/Lesion scraping, Ocular specimen Viral Transport Media  
Tracheal aspirate, BAL, Respiratory tract specimen Sterile container 0.4 mL

Inpatient Specimen Preparation

Throat swab, Genital specimen, Rectal swab, Skin/Lesion scraping, Ocular specimen, Tracheal aspirate, BAL, Respiratory tract specimen:
      Transport to laboratory as soon as possible

Unacceptable Conditions

Swabs transported without the use of Viral Transport Media, Diluted specimen, Thawed and refrozen specimen, Not received at proper temperature, Not received within specified time frame

Stability

Throat swab, Genital specimen, Rectal swab, Skin/Lesion scraping, Ocular specimen, Tracheal aspirate, BAL, Respiratory tract specimen: Room temperature 1 hour(s)

Throat swab, Genital specimen, Rectal swab, Skin/Lesion scraping, Ocular specimen:, Tracheal aspirate, BAL, Respiratory tract specimen: Refrigerated 4 day(s)

Throat swab, Genital specimen, Rectal swab, Skin/Lesion scraping, Ocular specimen:, Tracheal aspirate, BAL, Respiratory tract specimen: Frozen Indefinite week(s)
 

Remarks

Lesion specimens should contain cells. Break vesicle and scrape lesion to obtain adequate specimen. Swab specimens must be transported in Viral Transport Media.Viral Transport Media vials received without the swab may yield reduced sensitivity.

HSVTT2 detects both HSV-1 and HSV-2 and positive results are reported along with the type.

Days Performed

Daily

Set Up Schedule

Dayshift

Typical Turnaround

2 days

CPT Codes

87529

Lab Area

CMI, Molecular Infectious Disease

Synonyms

  • Herpes Simplex Virus by PCR, Superficial Site, HSV by PCR Superfical Site, HSV by PCR Superficial Qualitative
  • HSV qual
  • HSV qualitative
Overview/Billing

Synonyms

  • Herpes Simplex Virus by PCR, Superficial Site, HSV by PCR Superfical Site, HSV by PCR Superficial Qualitative
  • HSV qual
  • HSV qualitative

CPT Codes

87529
Interpretation

Methodology

Real-time Polymerase Chain Reaction (PCR)
NCH Lab Only

Collect

Specimen Type Type of Container Volume of Specimen Status
Throat swab Viral Transport Media   Preferred
Genital swab Viral Transport Media   Preferred
Rectal swab Viral Transport Media   Preferred
Skin/Lesion scraping Viral Transport Media   Preferred
Ocular specimen Viral Transport Media   Preferred
Tracheal aspirate Sterile container 1 mL Preferred
BAL Sterile container 1 mL Preferred
Respiratory tract specimen Sterile container 1 mL Preferred

Minimum Volume

Specimen Type Type of Container Minimum Volume
Throat swab, Genital swab, Rectal swab, Skin/Lesion scraping, Ocular specimen Viral Transport Media  
Tracheal aspirate, BAL, Respiratory tract specimen Sterile container 0.4 mL

Inpatient Specimen Preparation

Throat swab, Genital specimen, Rectal swab, Skin/Lesion scraping, Ocular specimen, Tracheal aspirate, BAL, Respiratory tract specimen:
      Transport to laboratory as soon as possible

Outpatient Specimen Preparation

Throat swab, Genital specimen, Rectal swab, Skin/Lesion scraping, Ocular specimen, Tracheal aspirate, BAL, Respiratory tract specimen:
      Transport to laboratory as soon as possible
      If delay in transport greater than 1 hour, refrigerate
 

InLab Processing

Samples received frozen must remain frozen until the time of testing.

Stability

Throat swab, Genital specimen, Rectal swab, Skin/Lesion scraping, Ocular specimen, Tracheal aspirate, BAL, Respiratory tract specimen: Room temperature 1 hour(s)

Throat swab, Genital specimen, Rectal swab, Skin/Lesion scraping, Ocular specimen:, Tracheal aspirate, BAL, Respiratory tract specimen: Refrigerated 4 day(s)

Throat swab, Genital specimen, Rectal swab, Skin/Lesion scraping, Ocular specimen:, Tracheal aspirate, BAL, Respiratory tract specimen: Frozen Indefinite week(s)
 

Unacceptable Conditions

Swabs transported without the use of Viral Transport Media, Diluted specimen, Thawed and refrozen specimen, Not received at proper temperature, Not received within specified time frame

Days Performed

Daily

Set Up Schedule

Dayshift

Typical Turnaround

2 days

Remarks

Lesion specimens should contain cells. Break vesicle and scrape lesion to obtain adequate specimen. Swab specimens must be transported in Viral Transport Media.Viral Transport Media vials received without the swab may yield reduced sensitivity.

HSVTT2 detects both HSV-1 and HSV-2 and positive results are reported along with the type.

Synonyms

  • Herpes Simplex Virus by PCR, Superficial Site, HSV by PCR Superfical Site, HSV by PCR Superficial Qualitative
  • HSV qual
  • HSV qualitative

Methodology

Real-time Polymerase Chain Reaction (PCR)

CPT Codes

87529

DC Code

5404

Downtime Availability

1-Continuous availability

Critical Information

Critical Specimen Handling Flag
Time sensitive handling required see stability section

Lab Area

Lab Area
CMI, Molecular Infectious Disease

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