Submission of a completed Prenatal Genetic Test Requisition Form is required. 

Please collect minimum of 15 mL amniotic fluid in a non-toxic, sterile container. Transport the sample at room temperature and send to the laboratory within 24 hours from collection time. DO NOT freeze the sample.

• If Aneuploidy FISH Screen for common aneuploidies (chromosomes 13, 18, 21, X and Y; see test code: GEN3) is also ordered, submit additional 5-10 mL amniotic fluid.
• If amniotic fluid AFP with reflex to AChE and fetal hemoglobin, infectious disease qualitative PCR, and/or 7-dehydrocholesterol biochemical study (Smith-Lemli-Opitz syndrome testing) is also ordered, submit 2-3 mL additional amniotic fluid. Although direct (unspun) fluid is always preferred, supernatant fluid can also be used as alternate sample for these tests.
• If Prenatal Microarray Analysis is also desired, please submit maternal sample (4 mL blood in EDTA tube) for maternal cell contamination study.
• If additional testing on cultured amniocytes is also ordered (e.g., send out cultured cells to another laboratory), submit additional 5-10 mL amniotic fluid and call Cytogenetics Lab to speak with a laboratory genetic counselor to coordinate. For molecular genetic testing to be performed on cultured amniocytes, submission of maternal sample (4 mL blood in EDTA tube) is also recommended to perform maternal cell contamination study.
• For sending out cultured amniocytes to another reference laboratory or to perform DNA isolation on cultured amniocytes, additional culture charge will be billed (under additional CPT code 88235).
• If cryopreservation of cultured cells is requested, a portion of cultured cells will be frozen and stored for possible future testing. Cyropreservation charge will apply (CPT code 88240).

In this study, cells in amniotic fluid specimen will be cultured, and the number of chromosomes will be evaluated in 5 cultured cells and the full karyotype analysis will be performed on 1 of the 5 cells. This test is available to confirm the results of previous cytogenetic testing performed in patient's current pregnancy, such as patients who had chromosome analysis and/or microarray analysis on chorionic villus sampling (CVS) specimen. Please submit a copy of the previous cytogenetic results (if available).

PLEASE NOTE: Due to the limited number of cells evaluated by this study for chromosome analysis, mosaicism for chromosomal abnormality may not be detected by this study. If mosaicism is suspected, an alternate testing called "Amniotic Fluid Chromosome Analysis (test code: AFST)" that evaluates 15 colonies is available, with an option for "Mosaicism Study" (CPT code 88263, extra charge applies) that evaluates additional number of colonies to evaluate for the presence of low-level mosaicism for chromosome abnormality.

Submission of a completed Prenatal Genetic Test Requisition Form is required. 

Please collect minimum of 15 mL amniotic fluid in a non-toxic, sterile container. Transport the sample at room temperature and send to the laboratory within 24 hours from collection time. DO NOT freeze the sample.

• If Aneuploidy FISH Screen for common aneuploidies (chromosomes 13, 18, 21, X and Y; see test code: GEN3) is also ordered, submit additional 5-10 mL amniotic fluid.
• If amniotic fluid AFP with reflex to AChE and fetal hemoglobin, infectious disease qualitative PCR, and/or 7-dehydrocholesterol biochemical study (Smith-Lemli-Opitz syndrome testing) is also ordered, submit 2-3 mL additional amniotic fluid. Although direct (unspun) fluid is always preferred, supernatant fluid can also be used as alternate sample for these tests.
• If Prenatal Microarray Analysis is also desired, please submit maternal sample (4 mL blood in EDTA tube) for maternal cell contamination study.
• If additional testing on cultured amniocytes is also ordered (e.g., send out cultured cells to another laboratory), submit additional 5-10 mL amniotic fluid and call Cytogenetics Lab to speak with a laboratory genetic counselor to coordinate. For molecular genetic testing to be performed on cultured amniocytes, submission of maternal sample (4 mL blood in EDTA tube) is also recommended to perform maternal cell contamination study.
• For sending out cultured amniocytes to another reference laboratory or to perform DNA isolation on cultured amniocytes, additional culture charge will be billed (under additional CPT code 88235).
• If cryopreservation of cultured cells is requested, a portion of cultured cells will be frozen and stored for possible future testing. Cyropreservation charge will apply (CPT code 88240).

In this study, cells in amniotic fluid specimen will be cultured, and the number of chromosomes will be evaluated in 5 cultured cells and the full karyotype analysis will be performed on 1 of the 5 cells. This test is available to confirm the results of previous cytogenetic testing performed in patient's current pregnancy, such as patients who had chromosome analysis and/or microarray analysis on chorionic villus sampling (CVS) specimen. Please submit a copy of the previous cytogenetic results (if available).

PLEASE NOTE: Due to the limited number of cells evaluated by this study for chromosome analysis, mosaicism for chromosomal abnormality may not be detected by this study. If mosaicism is suspected, an alternate testing called "Amniotic Fluid Chromosome Analysis (test code: AFST)" that evaluates 15 colonies is available, with an option for "Mosaicism Study" (CPT code 88263, extra charge applies) that evaluates additional number of colonies to evaluate for the presence of low-level mosaicism for chromosome abnormality.

In this study, cells in amniotic fluid specimen will be cultured, and the number of chromosomes will be evaluated in 5 cultured cells and the full karyotype analysis will be performed on 1 of the 5 cells. This test is available to confirm the results of previous cytogenetic testing performed in patient's current pregnancy, such as patients who had chromosome analysis and/or microarray analysis on chorionic villus sampling (CVS) specimen. Please submit a copy of the previous cytogenetic results (if available).

PLEASE NOTE: Due to the limited number of cells evaluated by this study for chromosome analysis, mosaicism for chromosomal abnormality may not be detected by this study. If mosaicism is suspected, an alternate testing called "Amniotic Fluid Chromosome Analysis (test code: AFST)" that evaluates 15 colonies is available, with an option for "Mosaicism Study" (CPT code 88263, extra charge applies) that evaluates additional number of colonies to evaluate for the presence of low-level mosaicism for chromosome abnormality.

Submission of a completed Prenatal Genetic Test Requisition Form is required. 

Please collect minimum of 15 mL amniotic fluid in a non-toxic, sterile container. Transport the sample at room temperature and send to the laboratory within 24 hours from collection time. DO NOT freeze the sample.

• If Aneuploidy FISH Screen for common aneuploidies (chromosomes 13, 18, 21, X and Y; see test code: GEN3) is also ordered, submit additional 5-10 mL amniotic fluid.
• If amniotic fluid AFP with reflex to AChE and fetal hemoglobin, infectious disease qualitative PCR, and/or 7-dehydrocholesterol biochemical study (Smith-Lemli-Opitz syndrome testing) is also ordered, submit 2-3 mL additional amniotic fluid. Although direct (unspun) fluid is always preferred, supernatant fluid can also be used as alternate sample for these tests.
• If Prenatal Microarray Analysis is also desired, please submit maternal sample (4 mL blood in EDTA tube) for maternal cell contamination study.
• If additional testing on cultured amniocytes is also ordered (e.g., send out cultured cells to another laboratory), submit additional 5-10 mL amniotic fluid and call Cytogenetics Lab to speak with a laboratory genetic counselor to coordinate. For molecular genetic testing to be performed on cultured amniocytes, submission of maternal sample (4 mL blood in EDTA tube) is also recommended to perform maternal cell contamination study.
• For sending out cultured amniocytes to another reference laboratory or to perform DNA isolation on cultured amniocytes, additional culture charge will be billed (under additional CPT code 88235).
• If cryopreservation of cultured cells is requested, a portion of cultured cells will be frozen and stored for possible future testing. Cyropreservation charge will apply (CPT code 88240).

In this study, cells in amniotic fluid specimen will be cultured, and the number of chromosomes will be evaluated in 5 cultured cells and the full karyotype analysis will be performed on 1 of the 5 cells. This test is available to confirm the results of previous cytogenetic testing performed in patient's current pregnancy, such as patients who had chromosome analysis and/or microarray analysis on chorionic villus sampling (CVS) specimen. Please submit a copy of the previous cytogenetic results (if available).

PLEASE NOTE: Due to the limited number of cells evaluated by this study for chromosome analysis, mosaicism for chromosomal abnormality may not be detected by this study. If mosaicism is suspected, an alternate testing called "Amniotic Fluid Chromosome Analysis (test code: AFST)" that evaluates 15 colonies is available, with an option for "Mosaicism Study" (CPT code 88263, extra charge applies) that evaluates additional number of colonies to evaluate for the presence of low-level mosaicism for chromosome abnormality.