| Specimen Type | Type of Container | Volume of Specimen | Status |
|---|---|---|---|
| Whole blood | 4 mL Green tube (Sodium heparin), No Gel | 4 mL | Preferred |
Sorted cells (from blood) | 4 mL (CD3/CD33) or 2x4 mL (CD3/CD33/CD19/CD56) Green tube (Sodium heparin), No Gel | See Remarks | Preferred |
Sorted cells (from blood) | 4 mL (CD3/CD33) or 2x4 mL (CD3/CD33/CD19/CD56) Purple tube (EDTA) | See Remarks | Alternate |
Whole blood: Do not centrifuge
Keep at room temperature
Do not freeze
Peripheral blood only, bone marrow not accepted
Sorted cells: See Remarks section for collection instructions
Specimen must be processed within 24 hours of collection
Peripheral blood only, bone marrow not accepted
Do not freeze
Do not centrifuge
Transport to the lab immediately after collection
This test is only available for patients who have had a single stem cell transplant using a sex-mismatched donor.
Submission of a completed BMT Engraftment/Chimerism Test Requisition Form is required; The requisition form must provide:
All samples should be labeled with specimen type (e.g. blood).
Whole blood testing must be performed from 4 mL Sodium Heparin (NaHep) blood.
SORTED CELLS ORDERING:
If analysis of sorted cells is requested, please indicate on the Test Requisition Form:
SORTED CELLS SAMPLE COLLECTION:
| Sorted Cell Types | Specimen Container & Volume | Collection Time |
|---|---|---|
| CD3/CD33 | Minimum of 4 mL peripheral blood in NaHep (green-top) or EDTA (purple top) tube | Monday-Thursday; Friday collection must be done on Nationwide Children's Main Campus |
| CD3/CD33/CD19/CD56 | Minimum of 2x4 mL peripheral blood in NaHep (green-top) or EDTA (purple top) tube | Monday-Thursday; Friday collection must be done on Nationwide Children's Main Campus |
Bone marrow engraftment analysis by FISH, also known as XX/XY chimerism testing, involves determining the percentage of donor cells present in the recipient after bone marrow transplant (BMT) or hematopoietic stem cell transplantation (HSCT). FISH for XX/XY signals on interphase cells is done to monitor BMT/HSCT engraftment in recipients subsequent to a transplant using a sex-mismatched donor.
For BMT engraftment monitoring through molecular (DNA) genetic markers, please see test code BMPT (Post-Transplant Analysis, Bone Marrow Engraftment). Please call Institute for Genetics Medicine Clinical Laboratory at (614) 722-5321 with questions.
| Specimen Type | Type of Container | Volume of Specimen | Status |
|---|---|---|---|
| Whole blood | 4 mL Green tube (Sodium heparin), No Gel | 4 mL | Preferred |
Sorted cells (from blood) | 4 mL (CD3/CD33) or 2x4 mL (CD3/CD33/CD19/CD56) Green tube (Sodium heparin), No Gel | See Remarks | Preferred |
Sorted cells (from blood) | 4 mL (CD3/CD33) or 2x4 mL (CD3/CD33/CD19/CD56) Purple tube (EDTA) | See Remarks | Alternate |
| Specimen Type | Type of Container | Volume of Specimen | Status |
|---|---|---|---|
| Whole blood | 4 mL Green tube (Sodium heparin), No Gel | 4 mL | Preferred |
| Sorted cells (from blood) | 4 mL (CD3/CD33) or 2x4 mL (CD3/CD33/CD19/CD56) Green tube (Sodium heparin), No Gel | See Remarks | Preferred |
| Sorted cells (from blood) | 4 mL (CD3/CD33) or 2x4 mL (CD3/CD33/CD19/CD56) Purple tube (EDTA) | See Remarks | Alternate |
Whole blood: Do not centrifuge
Keep at room temperature
Do not freeze
Peripheral blood only, bone marrow not accepted
Sorted cells: See Remarks section for collection instructions
Specimen must be processed within 24 hours of collection
Peripheral blood only, bone marrow not accepted
Do not freeze
Do not centrifuge
Transport to the lab immediately after collection
This test is only available for patients who have had a single stem cell transplant using a sex-mismatched donor.
Submission of a completed BMT Engraftment/Chimerism Test Requisition Form is required; The requisition form must provide:
All samples should be labeled with specimen type (e.g. blood).
Whole blood testing must be performed from 4 mL Sodium Heparin (NaHep) blood.
SORTED CELLS ORDERING:
If analysis of sorted cells is requested, please indicate on the Test Requisition Form:
SORTED CELLS SAMPLE COLLECTION:
| Sorted Cell Types | Specimen Container & Volume | Collection Time |
|---|---|---|
| CD3/CD33 | Minimum of 4 mL peripheral blood in NaHep (green-top) or EDTA (purple top) tube | Monday-Thursday; Friday collection must be done on Nationwide Children's Main Campus |
| CD3/CD33/CD19/CD56 | Minimum of 2x4 mL peripheral blood in NaHep (green-top) or EDTA (purple top) tube | Monday-Thursday; Friday collection must be done on Nationwide Children's Main Campus |
Bone marrow engraftment analysis by FISH, also known as XX/XY chimerism testing, involves determining the percentage of donor cells present in the recipient after bone marrow transplant (BMT) or hematopoietic stem cell transplantation (HSCT). FISH for XX/XY signals on interphase cells is done to monitor BMT/HSCT engraftment in recipients subsequent to a transplant using a sex-mismatched donor.
For BMT engraftment monitoring through molecular (DNA) genetic markers, please see test code BMPT (Post-Transplant Analysis, Bone Marrow Engraftment). Please call Institute for Genetics Medicine Clinical Laboratory at (614) 722-5321 with questions.
Bone marrow engraftment analysis by FISH, also known as XX/XY chimerism testing, involves determining the percentage of donor cells present in the recipient after bone marrow transplant (BMT) or hematopoietic stem cell transplantation (HSCT). FISH for XX/XY signals on interphase cells is done to monitor BMT/HSCT engraftment in recipients subsequent to a transplant using a sex-mismatched donor.
For BMT engraftment monitoring through molecular (DNA) genetic markers, please see test code BMPT (Post-Transplant Analysis, Bone Marrow Engraftment). Please call Institute for Genetics Medicine Clinical Laboratory at (614) 722-5321 with questions.
| Specimen Type | Type of Container | Volume of Specimen | Status |
|---|---|---|---|
| Whole blood | 4 mL Green tube (Sodium heparin), No Gel | 4 mL | Preferred |
Sorted cells (from blood) | 4 mL (CD3/CD33) or 2x4 mL (CD3/CD33/CD19/CD56) Green tube (Sodium heparin), No Gel | See Remarks | Preferred |
Sorted cells (from blood) | 4 mL (CD3/CD33) or 2x4 mL (CD3/CD33/CD19/CD56) Purple tube (EDTA) | See Remarks | Alternate |
| Specimen Type | Type of Container | Volume of Specimen | Status |
|---|---|---|---|
| Whole blood | 4 mL Green tube (Sodium heparin), No Gel | 4 mL | Preferred |
| Sorted cells (from blood) | 4 mL (CD3/CD33) or 2x4 mL (CD3/CD33/CD19/CD56) Green tube (Sodium heparin), No Gel | See Remarks | Preferred |
| Sorted cells (from blood) | 4 mL (CD3/CD33) or 2x4 mL (CD3/CD33/CD19/CD56) Purple tube (EDTA) | See Remarks | Alternate |
Whole blood: Do not centrifuge
Keep at room temperature
Do not freeze
Peripheral blood only, bone marrow not accepted
Sorted cells: See Remarks section for collection instructions
Specimen must be processed within 24 hours of collection
Peripheral blood only, bone marrow not accepted
Do not freeze
Do not centrifuge
Transport to the lab immediately after collection
Whole blood: Do not centrifuge
Keep at room temperature
Do not freeze
Peripheral blood only, bone marrow not accepted
Sorted cells: See Remarks section for collection instructions
Specimen must be processed within 24 hours of collection
Peripheral blood only, bone marrow not accepted
Do not freeze
Do not centrifuge
Transport to the lab immediately after collection
STAT/TIME SENSITIVE SPECIMEN. For Non-EPIC lab orders CPA needs to order SGENSP and XSORT in Sunquest. Send minimum 4mL NaHep or 4mL EDTA to send out lab with XSORT label. Send 4 mL NaHep to the Institute for Genomic Medicine with SGENSP. If questions please call IGM at 25321. Send out lab- see XSORT entry for OSU sorted cells requisition. If order states a CTL infusion has taken place, please reach out to IGM prior to sending sorted cells.
This test is only available for patients who have had a single stem cell transplant using a sex-mismatched donor.
Submission of a completed BMT Engraftment/Chimerism Test Requisition Form is required; The requisition form must provide:
All samples should be labeled with specimen type (e.g. blood).
Whole blood testing must be performed from 4 mL Sodium Heparin (NaHep) blood.
SORTED CELLS ORDERING:
If analysis of sorted cells is requested, please indicate on the Test Requisition Form:
SORTED CELLS SAMPLE COLLECTION:
| Sorted Cell Types | Specimen Container & Volume | Collection Time |
|---|---|---|
| CD3/CD33 | Minimum of 4 mL peripheral blood in NaHep (green-top) or EDTA (purple top) tube | Monday-Thursday; Friday collection must be done on Nationwide Children's Main Campus |
| CD3/CD33/CD19/CD56 | Minimum of 2x4 mL peripheral blood in NaHep (green-top) or EDTA (purple top) tube | Monday-Thursday; Friday collection must be done on Nationwide Children's Main Campus |
Bone marrow engraftment analysis by FISH, also known as XX/XY chimerism testing, involves determining the percentage of donor cells present in the recipient after bone marrow transplant (BMT) or hematopoietic stem cell transplantation (HSCT). FISH for XX/XY signals on interphase cells is done to monitor BMT/HSCT engraftment in recipients subsequent to a transplant using a sex-mismatched donor.
For BMT engraftment monitoring through molecular (DNA) genetic markers, please see test code BMPT (Post-Transplant Analysis, Bone Marrow Engraftment). Please call Institute for Genetics Medicine Clinical Laboratory at (614) 722-5321 with questions.
| Outpatient Requirements |
| Specimen Type | Type of Container | Volume of Specimen | Status |
|---|---|---|---|
| Whole blood | 4 mL Green tube (Sodium heparin), No Gel | 4 mL | Preferred |
Sorted cells (from blood) | 4 mL (CD3/CD33) or 2x4 mL (CD3/CD33/CD19/CD56) Green tube (Sodium heparin), No Gel | See Remarks | Preferred |
Sorted cells (from blood) | 4 mL (CD3/CD33) or 2x4 mL (CD3/CD33/CD19/CD56) Purple tube (EDTA) | See Remarks | Alternate |
Whole blood: Do not centrifuge
Keep at room temperature
Do not freeze
Peripheral blood only, bone marrow not accepted
Sorted cells: See Remarks section for collection instructions
Specimen must be processed within 24 hours of collection
Peripheral blood only, bone marrow not accepted
Do not freeze
Do not centrifuge
Transport to the lab immediately after collection
This test is only available for patients who have had a single stem cell transplant using a sex-mismatched donor.
Submission of a completed BMT Engraftment/Chimerism Test Requisition Form is required; The requisition form must provide:
All samples should be labeled with specimen type (e.g. blood).
Whole blood testing must be performed from 4 mL Sodium Heparin (NaHep) blood.
SORTED CELLS ORDERING:
If analysis of sorted cells is requested, please indicate on the Test Requisition Form:
SORTED CELLS SAMPLE COLLECTION:
| Sorted Cell Types | Specimen Container & Volume | Collection Time |
|---|---|---|
| CD3/CD33 | Minimum of 4 mL peripheral blood in NaHep (green-top) or EDTA (purple top) tube | Monday-Thursday; Friday collection must be done on Nationwide Children's Main Campus |
| CD3/CD33/CD19/CD56 | Minimum of 2x4 mL peripheral blood in NaHep (green-top) or EDTA (purple top) tube | Monday-Thursday; Friday collection must be done on Nationwide Children's Main Campus |
Bone marrow engraftment analysis by FISH, also known as XX/XY chimerism testing, involves determining the percentage of donor cells present in the recipient after bone marrow transplant (BMT) or hematopoietic stem cell transplantation (HSCT). FISH for XX/XY signals on interphase cells is done to monitor BMT/HSCT engraftment in recipients subsequent to a transplant using a sex-mismatched donor.
For BMT engraftment monitoring through molecular (DNA) genetic markers, please see test code BMPT (Post-Transplant Analysis, Bone Marrow Engraftment). Please call Institute for Genetics Medicine Clinical Laboratory at (614) 722-5321 with questions.
| Inpatient Requirements |
| Specimen Type | Type of Container | Volume of Specimen | Status |
|---|---|---|---|
| Whole blood | 4 mL Green tube (Sodium heparin), No Gel | 4 mL | Preferred |
Sorted cells (from blood) | 4 mL (CD3/CD33) or 2x4 mL (CD3/CD33/CD19/CD56) Green tube (Sodium heparin), No Gel | See Remarks | Preferred |
Sorted cells (from blood) | 4 mL (CD3/CD33) or 2x4 mL (CD3/CD33/CD19/CD56) Purple tube (EDTA) | See Remarks | Alternate |
| Specimen Type | Type of Container | Volume of Specimen | Status |
|---|---|---|---|
| Whole blood | 4 mL Green tube (Sodium heparin), No Gel | 4 mL | Preferred |
| Sorted cells (from blood) | 4 mL (CD3/CD33) or 2x4 mL (CD3/CD33/CD19/CD56) Green tube (Sodium heparin), No Gel | See Remarks | Preferred |
| Sorted cells (from blood) | 4 mL (CD3/CD33) or 2x4 mL (CD3/CD33/CD19/CD56) Purple tube (EDTA) | See Remarks | Alternate |
Whole blood: Do not centrifuge
Keep at room temperature
Do not freeze
Peripheral blood only, bone marrow not accepted
Sorted cells: See Remarks section for collection instructions
Specimen must be processed within 24 hours of collection
Peripheral blood only, bone marrow not accepted
Do not freeze
Do not centrifuge
Transport to the lab immediately after collection
This test is only available for patients who have had a single stem cell transplant using a sex-mismatched donor.
Submission of a completed BMT Engraftment/Chimerism Test Requisition Form is required; The requisition form must provide:
All samples should be labeled with specimen type (e.g. blood).
Whole blood testing must be performed from 4 mL Sodium Heparin (NaHep) blood.
SORTED CELLS ORDERING:
If analysis of sorted cells is requested, please indicate on the Test Requisition Form:
SORTED CELLS SAMPLE COLLECTION:
| Sorted Cell Types | Specimen Container & Volume | Collection Time |
|---|---|---|
| CD3/CD33 | Minimum of 4 mL peripheral blood in NaHep (green-top) or EDTA (purple top) tube | Monday-Thursday; Friday collection must be done on Nationwide Children's Main Campus |
| CD3/CD33/CD19/CD56 | Minimum of 2x4 mL peripheral blood in NaHep (green-top) or EDTA (purple top) tube | Monday-Thursday; Friday collection must be done on Nationwide Children's Main Campus |
Bone marrow engraftment analysis by FISH, also known as XX/XY chimerism testing, involves determining the percentage of donor cells present in the recipient after bone marrow transplant (BMT) or hematopoietic stem cell transplantation (HSCT). FISH for XX/XY signals on interphase cells is done to monitor BMT/HSCT engraftment in recipients subsequent to a transplant using a sex-mismatched donor.
For BMT engraftment monitoring through molecular (DNA) genetic markers, please see test code BMPT (Post-Transplant Analysis, Bone Marrow Engraftment). Please call Institute for Genetics Medicine Clinical Laboratory at (614) 722-5321 with questions.
| Overview/Billing |
| Interpretation |
Bone marrow engraftment analysis by FISH, also known as XX/XY chimerism testing, involves determining the percentage of donor cells present in the recipient after bone marrow transplant (BMT) or hematopoietic stem cell transplantation (HSCT). FISH for XX/XY signals on interphase cells is done to monitor BMT/HSCT engraftment in recipients subsequent to a transplant using a sex-mismatched donor.
For BMT engraftment monitoring through molecular (DNA) genetic markers, please see test code BMPT (Post-Transplant Analysis, Bone Marrow Engraftment). Please call Institute for Genetics Medicine Clinical Laboratory at (614) 722-5321 with questions.
| NCH Lab Only |
| Specimen Type | Type of Container | Volume of Specimen | Status |
|---|---|---|---|
| Whole blood | 4 mL Green tube (Sodium heparin), No Gel | 4 mL | Preferred |
Sorted cells (from blood) | 4 mL (CD3/CD33) or 2x4 mL (CD3/CD33/CD19/CD56) Green tube (Sodium heparin), No Gel | See Remarks | Preferred |
Sorted cells (from blood) | 4 mL (CD3/CD33) or 2x4 mL (CD3/CD33/CD19/CD56) Purple tube (EDTA) | See Remarks | Alternate |
| Specimen Type | Type of Container | Volume of Specimen | Status |
|---|---|---|---|
| Whole blood | 4 mL Green tube (Sodium heparin), No Gel | 4 mL | Preferred |
| Sorted cells (from blood) | 4 mL (CD3/CD33) or 2x4 mL (CD3/CD33/CD19/CD56) Green tube (Sodium heparin), No Gel | See Remarks | Preferred |
| Sorted cells (from blood) | 4 mL (CD3/CD33) or 2x4 mL (CD3/CD33/CD19/CD56) Purple tube (EDTA) | See Remarks | Alternate |
Whole blood: Do not centrifuge
Keep at room temperature
Do not freeze
Peripheral blood only, bone marrow not accepted
Sorted cells: See Remarks section for collection instructions
Specimen must be processed within 24 hours of collection
Peripheral blood only, bone marrow not accepted
Do not freeze
Do not centrifuge
Transport to the lab immediately after collection
Whole blood: Do not centrifuge
Keep at room temperature
Do not freeze
Peripheral blood only, bone marrow not accepted
Sorted cells: See Remarks section for collection instructions
Specimen must be processed within 24 hours of collection
Peripheral blood only, bone marrow not accepted
Do not freeze
Do not centrifuge
Transport to the lab immediately after collection
STAT/TIME SENSITIVE SPECIMEN. For Non-EPIC lab orders CPA needs to order SGENSP and XSORT in Sunquest. Send minimum 4mL NaHep or 4mL EDTA to send out lab with XSORT label. Send 4 mL NaHep to the Institute for Genomic Medicine with SGENSP. If questions please call IGM at 25321. Send out lab- see XSORT entry for OSU sorted cells requisition. If order states a CTL infusion has taken place, please reach out to IGM prior to sending sorted cells.
This test is only available for patients who have had a single stem cell transplant using a sex-mismatched donor.
Submission of a completed BMT Engraftment/Chimerism Test Requisition Form is required; The requisition form must provide:
All samples should be labeled with specimen type (e.g. blood).
Whole blood testing must be performed from 4 mL Sodium Heparin (NaHep) blood.
SORTED CELLS ORDERING:
If analysis of sorted cells is requested, please indicate on the Test Requisition Form:
SORTED CELLS SAMPLE COLLECTION:
| Sorted Cell Types | Specimen Container & Volume | Collection Time |
|---|---|---|
| CD3/CD33 | Minimum of 4 mL peripheral blood in NaHep (green-top) or EDTA (purple top) tube | Monday-Thursday; Friday collection must be done on Nationwide Children's Main Campus |
| CD3/CD33/CD19/CD56 | Minimum of 2x4 mL peripheral blood in NaHep (green-top) or EDTA (purple top) tube | Monday-Thursday; Friday collection must be done on Nationwide Children's Main Campus |
Bone marrow engraftment analysis by FISH, also known as XX/XY chimerism testing, involves determining the percentage of donor cells present in the recipient after bone marrow transplant (BMT) or hematopoietic stem cell transplantation (HSCT). FISH for XX/XY signals on interphase cells is done to monitor BMT/HSCT engraftment in recipients subsequent to a transplant using a sex-mismatched donor.
For BMT engraftment monitoring through molecular (DNA) genetic markers, please see test code BMPT (Post-Transplant Analysis, Bone Marrow Engraftment). Please call Institute for Genetics Medicine Clinical Laboratory at (614) 722-5321 with questions.
