Special Collection

At least 50% blasts must be present in Hematologic Cancer samples. At least 40% tumor must be present in Solid Tumor samples (based on internal pathology review). Copy of previous tumor sequencing result is REQUIRED.

 

Outpatient Submit with Specimen

Oncology Genetic Requisition

Collect

Specimen Type Type of Container Volume of Specimen Status
Tissue (Snap-frozen) Tissue cassette 5mm x 5mm or larger Preferred
Tissue (Snap-frozen) Cryogenic tube 5mm x 5mm or larger Alternate
Bone marrow 4 mL Purple tube (EDTA) 4 mL Preferred
OCT-embedded tissue Tissue cassette 5mm x 5mm or larger Preferred
OCT-embedded tissue Cryogenic tube 5mm x 5mm or larger Alternate
Paraffin embedded tissue Paraffin block   Preferred
Tissue (Fresh) Tissue culture transport media 5mm x 5mm or larger Preferred
Tissue (Fresh) Sterile container with saline 5mm x 5mm or larger Alternate
Tissue scrolls (FFPE) Sterile container 10 scrolls at 15 microns thick Preferred
Tissue scrolls (OCT) Cryogenic tube 10 scrolls at 15 microns thick Preferred

Outpatient Specimen Preparation

Tissue (Snap-frozen): Keep frozen
                                   Protect from heat
                                   Transport to laboratory as soon as possible
                                   Immediately place on dry ice and transport frozen
                                   Tumor sample must contain a minimum of 40% tumor

Bone marrow: Do not freeze
                       Keep at room temperature or refrigerate
                       Do not centrifuge
                       Tumor sample must contain a minimum of 50% tumor

OCT-embedded tissue: Keep frozen
                                      Protect from heat
                                      Immediately place on dry ice and transport frozen
                                      Transport to laboratory as soon as possible
                                      Tumor sample must contain a minimum of 40% tumor

Paraffin embedded tissue: Protect from heat
                                          Keep at room temperature

Tissue (Fresh): Do not add fixative
                         Do not freeze
                         Keep at room temperature or refrigerate
                         Transport to laboratory as soon as possible

Tissue scrolls (FFPE): Keep at room temperature
                                    Protect from heat
                                    Must accompany H&E slide from the same tissue block used to make FFPE scrolls

Tissue scrolls (OCT): Keep frozen
                                  Protect from heat
                                  Immediately place on dry ice and transport frozen
                                  Transport to laboratory as soon as possible
                                  Must accompany H&E slide from the same OCT-embedded tissue used to make OCT                                              tissue scrolls

Unacceptable Conditions

Inadequate tissue, Tissue degradation, Delayed or improper handling, Wrong type of specimen

Stability

Tissue (Snap-frozen): Frozen 12 month(s)

Bone marrow: Room temperature 24 hour(s)
Bone marrow: Refrigerated 72 hour(s)

OCT-embedded tissue: Frozen 12 month(s)

Paraffin embedded tissue: Room temperature 6 month(s)

Tissue (Fresh): Room temperature 24 hour(s)
Tissue (Fresh): Refrigerated 48 hour(s)

Tissue scrolls (FFPE): Room temperature 6 month(s)
Tissue scrolls (OCT): Frozen 12 month(s)

Remarks

If you are an external healthcare provider with no access to Nationwide Children’s Epic system, submission of a completed Oncology Genetic Test Requisition Form is required. If you are an internal ordering provider with access to Nationwide Children’s Epic system, no requisition form is required; please place the lab order electronically in Epic.

If submitting tissue scroll specimen, please use this Tissue Scroll Calculator tool to determine the thickness and number of tissue scrolls to send to the laboratory.

For liquid tumor, sample must contain at least 50% blasts by internal pathology review. For solid tumor, sample must contain at least 40% tumor by internal pathology review. Submission of a normal sample containing 0% tumor is recommended, but not required, to rule out germline origin of the variant. Please send frozen samples (e.g. snap-frozen tissue or OCT-embedded tissue) buried in adequate amount of dry ice and ship by overnight courier. Formalin-fixed paraffin embedded (FFPE) tissue blocks and FFPE tissue scrolls can be transported at room temperature.

For questions, please call (614) 722-5321 and ask to speak with a lab genetic counselor.

Clinical Information

This test is a targeted Sanger sequencing analysis used to confirm the presence of a specific DNA sequence change in tumor sample that was previously identified by a research testing or testing performed by another clinical laboratory. This test is only available as a CONFIRMATORY TEST and only offered to patients who have a known somatic sequence variant(s) detected by previous research or clinical testing. If patient does not have a previously detected somatic variant(s), then this test cannot be ordered and samples will be rejected.

 Please submit a copy of the previous tumor sequencing result, if available.

Days Performed

Monday through Saturday

Set Up Schedule

All tests not performed daily.

Typical Turnaround

3 weeks

Lab Area

Institute for Genomic Medicine

Methodology

DNA extraction, Polymerase chain reaction (PCR), Sanger sequencing

CPT Codes

81479

Synonyms

  • Targeted tumor mutation analysis, Targeted cancer mutation confirmation, Known tumor mutation testing, Targeted somatic mutation testing, Single site tumor mutation analysis, Tumor mutation Sanger confirmation, Targeted tumor Sanger sequencing, Confirmatory tumor mutation testing, Targeted Sanger sequencing for tumor, IGM Test
  • RT-PCR Fusion Transcript Analysis, fusion, Pediatric Solid Tumors, ARMS, PNET, Sarcoma, Ewing sarcoma, Alveolar rhabdomyosarcoma, Synovial sarcoma, Desmoplastic small round cell tumor, DSRCT, Congenital fibrosarcoma, Cellular mesoblastic nephroma, Primitive neuroectodermal tumor, Soft tissue sarcoma fusion transcript, Sarcoma RT-PCR, RT-PCR, EWSR1, FLI1, FLI-1, ERG, EWS, PAX3, FOXO1, PAX7, SYT, SSX1, SS18, SSX2, WT1, ETV6, NTRK3, Sarcoma gene fusion panel, Sarcoma translocation, IGM Test

Special Collection

At least 50% blasts must be present in Hematologic Cancer samples. At least 40% tumor must be present in Solid Tumor samples (based on internal pathology review). Copy of previous tumor sequencing result is REQUIRED.

 

Collect

Specimen Type Type of Container Volume of Specimen Status
Tissue (Snap-frozen) Tissue cassette 5mm x 5mm or larger Preferred
Tissue (Snap-frozen) Cryogenic tube 5mm x 5mm or larger Alternate
Bone marrow 4 mL Purple tube (EDTA) 4 mL Preferred
OCT-embedded tissue Tissue cassette 5mm x 5mm or larger Preferred
OCT-embedded tissue Cryogenic tube 5mm x 5mm or larger Alternate
Paraffin embedded tissue Paraffin block   Preferred
Tissue (Fresh) Tissue culture transport media 5mm x 5mm or larger Preferred
Tissue (Fresh) Sterile container with saline 5mm x 5mm or larger Alternate
Tissue scrolls (FFPE) Sterile container 10 scrolls at 15 microns thick Preferred
Tissue scrolls (OCT) Cryogenic tube 10 scrolls at 15 microns thick Preferred

Minimum Volume

Specimen Type Type of Container Minimum Volume
Tissue (Snap-frozen) Tissue cassette 5mm x 5mm
Tissue (Snap-frozen) Cryogenic tube 5mm x 5mm
Bone marrow 4 mL Purple tube (EDTA) 2 mL
OCT-embedded tissue Tissue cassette 5mm x 5mm
OCT-embedded tissue Cryogenic tube 5mm x 5mm
Paraffin embedded tissue Paraffin block  
Tissue (Fresh) Tissue culture transport media 5mm x 5mm
Tissue (Fresh) Sterile container with saline 5mm x 5mm
Tissue scrolls (FFPE) Sterile container 10 scrolls 15 micron thick
Tissue scrolls (OCT) Cryogenic tube 10 scrolls 15 micron thick

Inpatient Specimen Preparation

Tissue (Snap-frozen): Keep frozen
                                   Protect from heat
                                   Transport to laboratory as soon as possible
                                   Immediately place on dry ice and transport frozen
                                   Tumor sample must contain a minimum of 40% tumor

Bone marrow: Do not freeze
                       Keep at room temperature or refrigerate
                       Do not centrifuge
                       Tumor sample must contain a minimum of 50% tumor

OCT-embedded tissue: Keep frozen
                                      Protect from heat
                                      Immediately place on dry ice and transport frozen
                                      Transport to laboratory as soon as possible
                                      Tumor sample must contain a minimum of 40% tumor

Paraffin embedded tissue: Protect from heat
                                          Keep at room temperature

Tissue (Fresh): Do not add fixative
                         Do not freeze
                         Keep at room temperature or refrigerate
                         Transport to laboratory as soon as possible

Tissue scrolls (FFPE): Keep at room temperature
                                    Protect from heat
                                    Must accompany H&E slide from the same tissue block used to make FFPE scrolls

Tissue scrolls (OCT): Keep frozen
                                  Protect from heat
                                  Immediately place on dry ice and transport frozen
                                  Transport to laboratory as soon as possible
                                  Must accompany H&E slide from the same OCT-embedded tissue used to make OCT                                              tissue scrolls

Unacceptable Conditions

Inadequate tissue, Tissue degradation, Delayed or improper handling, Wrong type of specimen

Stability

Tissue (Snap-frozen): Frozen 12 month(s)

Bone marrow: Room temperature 24 hour(s)
Bone marrow: Refrigerated 72 hour(s)

OCT-embedded tissue: Frozen 12 month(s)

Paraffin embedded tissue: Room temperature 6 month(s)

Tissue (Fresh): Room temperature 24 hour(s)
Tissue (Fresh): Refrigerated 48 hour(s)

Tissue scrolls (FFPE): Room temperature 6 month(s)
Tissue scrolls (OCT): Frozen 12 month(s)

Remarks

If you are an external healthcare provider with no access to Nationwide Children’s Epic system, submission of a completed Oncology Genetic Test Requisition Form is required. If you are an internal ordering provider with access to Nationwide Children’s Epic system, no requisition form is required; please place the lab order electronically in Epic.

If submitting tissue scroll specimen, please use this Tissue Scroll Calculator tool to determine the thickness and number of tissue scrolls to send to the laboratory.

For liquid tumor, sample must contain at least 50% blasts by internal pathology review. For solid tumor, sample must contain at least 40% tumor by internal pathology review. Submission of a normal sample containing 0% tumor is recommended, but not required, to rule out germline origin of the variant. Please send frozen samples (e.g. snap-frozen tissue or OCT-embedded tissue) buried in adequate amount of dry ice and ship by overnight courier. Formalin-fixed paraffin embedded (FFPE) tissue blocks and FFPE tissue scrolls can be transported at room temperature.

For questions, please call (614) 722-5321 and ask to speak with a lab genetic counselor.

Clinical Information

This test is a targeted Sanger sequencing analysis used to confirm the presence of a specific DNA sequence change in tumor sample that was previously identified by a research testing or testing performed by another clinical laboratory. This test is only available as a CONFIRMATORY TEST and only offered to patients who have a known somatic sequence variant(s) detected by previous research or clinical testing. If patient does not have a previously detected somatic variant(s), then this test cannot be ordered and samples will be rejected.

 Please submit a copy of the previous tumor sequencing result, if available.

Days Performed

Monday through Saturday

Set Up Schedule

All tests not performed daily.

Typical Turnaround

3 weeks

CPT Codes

81479

Lab Area

Institute for Genomic Medicine

Synonyms

  • Targeted tumor mutation analysis, Targeted cancer mutation confirmation, Known tumor mutation testing, Targeted somatic mutation testing, Single site tumor mutation analysis, Tumor mutation Sanger confirmation, Targeted tumor Sanger sequencing, Confirmatory tumor mutation testing, Targeted Sanger sequencing for tumor, IGM Test
  • RT-PCR Fusion Transcript Analysis, fusion, Pediatric Solid Tumors, ARMS, PNET, Sarcoma, Ewing sarcoma, Alveolar rhabdomyosarcoma, Synovial sarcoma, Desmoplastic small round cell tumor, DSRCT, Congenital fibrosarcoma, Cellular mesoblastic nephroma, Primitive neuroectodermal tumor, Soft tissue sarcoma fusion transcript, Sarcoma RT-PCR, RT-PCR, EWSR1, FLI1, FLI-1, ERG, EWS, PAX3, FOXO1, PAX7, SYT, SSX1, SS18, SSX2, WT1, ETV6, NTRK3, Sarcoma gene fusion panel, Sarcoma translocation, IGM Test

Estimated Patient Price

< $1,000

Synonyms

  • Targeted tumor mutation analysis, Targeted cancer mutation confirmation, Known tumor mutation testing, Targeted somatic mutation testing, Single site tumor mutation analysis, Tumor mutation Sanger confirmation, Targeted tumor Sanger sequencing, Confirmatory tumor mutation testing, Targeted Sanger sequencing for tumor, IGM Test
  • RT-PCR Fusion Transcript Analysis, fusion, Pediatric Solid Tumors, ARMS, PNET, Sarcoma, Ewing sarcoma, Alveolar rhabdomyosarcoma, Synovial sarcoma, Desmoplastic small round cell tumor, DSRCT, Congenital fibrosarcoma, Cellular mesoblastic nephroma, Primitive neuroectodermal tumor, Soft tissue sarcoma fusion transcript, Sarcoma RT-PCR, RT-PCR, EWSR1, FLI1, FLI-1, ERG, EWS, PAX3, FOXO1, PAX7, SYT, SSX1, SS18, SSX2, WT1, ETV6, NTRK3, Sarcoma gene fusion panel, Sarcoma translocation, IGM Test

CPT Codes

81479

Clinical Information

This test is a targeted Sanger sequencing analysis used to confirm the presence of a specific DNA sequence change in tumor sample that was previously identified by a research testing or testing performed by another clinical laboratory. This test is only available as a CONFIRMATORY TEST and only offered to patients who have a known somatic sequence variant(s) detected by previous research or clinical testing. If patient does not have a previously detected somatic variant(s), then this test cannot be ordered and samples will be rejected.

 Please submit a copy of the previous tumor sequencing result, if available.

Methodology

DNA extraction, Polymerase chain reaction (PCR), Sanger sequencing

Special Collection

At least 50% blasts must be present in Hematologic Cancer samples. At least 40% tumor must be present in Solid Tumor samples (based on internal pathology review). Copy of previous tumor sequencing result is REQUIRED.

 

Outpatient Submit with Specimen

Oncology Genetic Requisition

Collect

Specimen Type Type of Container Volume of Specimen Status
Tissue (Snap-frozen) Tissue cassette 5mm x 5mm or larger Preferred
Tissue (Snap-frozen) Cryogenic tube 5mm x 5mm or larger Alternate
Bone marrow 4 mL Purple tube (EDTA) 4 mL Preferred
OCT-embedded tissue Tissue cassette 5mm x 5mm or larger Preferred
OCT-embedded tissue Cryogenic tube 5mm x 5mm or larger Alternate
Paraffin embedded tissue Paraffin block   Preferred
Tissue (Fresh) Tissue culture transport media 5mm x 5mm or larger Preferred
Tissue (Fresh) Sterile container with saline 5mm x 5mm or larger Alternate
Tissue scrolls (FFPE) Sterile container 10 scrolls at 15 microns thick Preferred
Tissue scrolls (OCT) Cryogenic tube 10 scrolls at 15 microns thick Preferred

Minimum Volume

Specimen Type Type of Container Minimum Volume
Tissue (Snap-frozen) Tissue cassette 5mm x 5mm
Tissue (Snap-frozen) Cryogenic tube 5mm x 5mm
Bone marrow 4 mL Purple tube (EDTA) 2 mL
OCT-embedded tissue Tissue cassette 5mm x 5mm
OCT-embedded tissue Cryogenic tube 5mm x 5mm
Paraffin embedded tissue Paraffin block  
Tissue (Fresh) Tissue culture transport media 5mm x 5mm
Tissue (Fresh) Sterile container with saline 5mm x 5mm
Tissue scrolls (FFPE) Sterile container 10 scrolls 15 micron thick
Tissue scrolls (OCT) Cryogenic tube 10 scrolls 15 micron thick

Inpatient Specimen Preparation

Tissue (Snap-frozen): Keep frozen
                                   Protect from heat
                                   Transport to laboratory as soon as possible
                                   Immediately place on dry ice and transport frozen
                                   Tumor sample must contain a minimum of 40% tumor

Bone marrow: Do not freeze
                       Keep at room temperature or refrigerate
                       Do not centrifuge
                       Tumor sample must contain a minimum of 50% tumor

OCT-embedded tissue: Keep frozen
                                      Protect from heat
                                      Immediately place on dry ice and transport frozen
                                      Transport to laboratory as soon as possible
                                      Tumor sample must contain a minimum of 40% tumor

Paraffin embedded tissue: Protect from heat
                                          Keep at room temperature

Tissue (Fresh): Do not add fixative
                         Do not freeze
                         Keep at room temperature or refrigerate
                         Transport to laboratory as soon as possible

Tissue scrolls (FFPE): Keep at room temperature
                                    Protect from heat
                                    Must accompany H&E slide from the same tissue block used to make FFPE scrolls

Tissue scrolls (OCT): Keep frozen
                                  Protect from heat
                                  Immediately place on dry ice and transport frozen
                                  Transport to laboratory as soon as possible
                                  Must accompany H&E slide from the same OCT-embedded tissue used to make OCT                                              tissue scrolls

Outpatient Specimen Preparation

Tissue (Snap-frozen): Keep frozen
                                   Protect from heat
                                   Transport to laboratory as soon as possible
                                   Immediately place on dry ice and transport frozen
                                   Tumor sample must contain a minimum of 40% tumor

Bone marrow: Do not freeze
                       Keep at room temperature or refrigerate
                       Do not centrifuge
                       Tumor sample must contain a minimum of 50% tumor

OCT-embedded tissue: Keep frozen
                                      Protect from heat
                                      Immediately place on dry ice and transport frozen
                                      Transport to laboratory as soon as possible
                                      Tumor sample must contain a minimum of 40% tumor

Paraffin embedded tissue: Protect from heat
                                          Keep at room temperature

Tissue (Fresh): Do not add fixative
                         Do not freeze
                         Keep at room temperature or refrigerate
                         Transport to laboratory as soon as possible

Tissue scrolls (FFPE): Keep at room temperature
                                    Protect from heat
                                    Must accompany H&E slide from the same tissue block used to make FFPE scrolls

Tissue scrolls (OCT): Keep frozen
                                  Protect from heat
                                  Immediately place on dry ice and transport frozen
                                  Transport to laboratory as soon as possible
                                  Must accompany H&E slide from the same OCT-embedded tissue used to make OCT                                              tissue scrolls

InLab Processing

Send to Molecular Genetics Lab with all submitted paperwork. For Non-EPIC lab orders, lab staff to order GENSP in Sunqest.

Stability

Tissue (Snap-frozen): Frozen 12 month(s)

Bone marrow: Room temperature 24 hour(s)
Bone marrow: Refrigerated 72 hour(s)

OCT-embedded tissue: Frozen 12 month(s)

Paraffin embedded tissue: Room temperature 6 month(s)

Tissue (Fresh): Room temperature 24 hour(s)
Tissue (Fresh): Refrigerated 48 hour(s)

Tissue scrolls (FFPE): Room temperature 6 month(s)
Tissue scrolls (OCT): Frozen 12 month(s)

Unacceptable Conditions

Inadequate tissue, Tissue degradation, Delayed or improper handling, Wrong type of specimen

Days Performed

Monday through Saturday

Set Up Schedule

All tests not performed daily.

Typical Turnaround

3 weeks

Remarks

If you are an external healthcare provider with no access to Nationwide Children’s Epic system, submission of a completed Oncology Genetic Test Requisition Form is required. If you are an internal ordering provider with access to Nationwide Children’s Epic system, no requisition form is required; please place the lab order electronically in Epic.

If submitting tissue scroll specimen, please use this Tissue Scroll Calculator tool to determine the thickness and number of tissue scrolls to send to the laboratory.

For liquid tumor, sample must contain at least 50% blasts by internal pathology review. For solid tumor, sample must contain at least 40% tumor by internal pathology review. Submission of a normal sample containing 0% tumor is recommended, but not required, to rule out germline origin of the variant. Please send frozen samples (e.g. snap-frozen tissue or OCT-embedded tissue) buried in adequate amount of dry ice and ship by overnight courier. Formalin-fixed paraffin embedded (FFPE) tissue blocks and FFPE tissue scrolls can be transported at room temperature.

For questions, please call (614) 722-5321 and ask to speak with a lab genetic counselor.

Clinical Information

This test is a targeted Sanger sequencing analysis used to confirm the presence of a specific DNA sequence change in tumor sample that was previously identified by a research testing or testing performed by another clinical laboratory. This test is only available as a CONFIRMATORY TEST and only offered to patients who have a known somatic sequence variant(s) detected by previous research or clinical testing. If patient does not have a previously detected somatic variant(s), then this test cannot be ordered and samples will be rejected.

 Please submit a copy of the previous tumor sequencing result, if available.

Synonyms

  • Targeted tumor mutation analysis, Targeted cancer mutation confirmation, Known tumor mutation testing, Targeted somatic mutation testing, Single site tumor mutation analysis, Tumor mutation Sanger confirmation, Targeted tumor Sanger sequencing, Confirmatory tumor mutation testing, Targeted Sanger sequencing for tumor, IGM Test
  • RT-PCR Fusion Transcript Analysis, fusion, Pediatric Solid Tumors, ARMS, PNET, Sarcoma, Ewing sarcoma, Alveolar rhabdomyosarcoma, Synovial sarcoma, Desmoplastic small round cell tumor, DSRCT, Congenital fibrosarcoma, Cellular mesoblastic nephroma, Primitive neuroectodermal tumor, Soft tissue sarcoma fusion transcript, Sarcoma RT-PCR, RT-PCR, EWSR1, FLI1, FLI-1, ERG, EWS, PAX3, FOXO1, PAX7, SYT, SSX1, SS18, SSX2, WT1, ETV6, NTRK3, Sarcoma gene fusion panel, Sarcoma translocation, IGM Test

Methodology

DNA extraction, Polymerase chain reaction (PCR), Sanger sequencing

CPT Codes

81479

Estimated Patient Price

< $1,000

DC Code

5321

Downtime Availability

4-Not available
Outpatient Requirements

Special Collection

At least 50% blasts must be present in Hematologic Cancer samples. At least 40% tumor must be present in Solid Tumor samples (based on internal pathology review). Copy of previous tumor sequencing result is REQUIRED.

 

Outpatient Submit with Specimen

Oncology Genetic Requisition

Collect

Specimen Type Type of Container Volume of Specimen Status
Tissue (Snap-frozen) Tissue cassette 5mm x 5mm or larger Preferred
Tissue (Snap-frozen) Cryogenic tube 5mm x 5mm or larger Alternate
Bone marrow 4 mL Purple tube (EDTA) 4 mL Preferred
OCT-embedded tissue Tissue cassette 5mm x 5mm or larger Preferred
OCT-embedded tissue Cryogenic tube 5mm x 5mm or larger Alternate
Paraffin embedded tissue Paraffin block   Preferred
Tissue (Fresh) Tissue culture transport media 5mm x 5mm or larger Preferred
Tissue (Fresh) Sterile container with saline 5mm x 5mm or larger Alternate
Tissue scrolls (FFPE) Sterile container 10 scrolls at 15 microns thick Preferred
Tissue scrolls (OCT) Cryogenic tube 10 scrolls at 15 microns thick Preferred

Outpatient Specimen Preparation

Tissue (Snap-frozen): Keep frozen
                                   Protect from heat
                                   Transport to laboratory as soon as possible
                                   Immediately place on dry ice and transport frozen
                                   Tumor sample must contain a minimum of 40% tumor

Bone marrow: Do not freeze
                       Keep at room temperature or refrigerate
                       Do not centrifuge
                       Tumor sample must contain a minimum of 50% tumor

OCT-embedded tissue: Keep frozen
                                      Protect from heat
                                      Immediately place on dry ice and transport frozen
                                      Transport to laboratory as soon as possible
                                      Tumor sample must contain a minimum of 40% tumor

Paraffin embedded tissue: Protect from heat
                                          Keep at room temperature

Tissue (Fresh): Do not add fixative
                         Do not freeze
                         Keep at room temperature or refrigerate
                         Transport to laboratory as soon as possible

Tissue scrolls (FFPE): Keep at room temperature
                                    Protect from heat
                                    Must accompany H&E slide from the same tissue block used to make FFPE scrolls

Tissue scrolls (OCT): Keep frozen
                                  Protect from heat
                                  Immediately place on dry ice and transport frozen
                                  Transport to laboratory as soon as possible
                                  Must accompany H&E slide from the same OCT-embedded tissue used to make OCT                                              tissue scrolls

Unacceptable Conditions

Inadequate tissue, Tissue degradation, Delayed or improper handling, Wrong type of specimen

Stability

Tissue (Snap-frozen): Frozen 12 month(s)

Bone marrow: Room temperature 24 hour(s)
Bone marrow: Refrigerated 72 hour(s)

OCT-embedded tissue: Frozen 12 month(s)

Paraffin embedded tissue: Room temperature 6 month(s)

Tissue (Fresh): Room temperature 24 hour(s)
Tissue (Fresh): Refrigerated 48 hour(s)

Tissue scrolls (FFPE): Room temperature 6 month(s)
Tissue scrolls (OCT): Frozen 12 month(s)

Remarks

If you are an external healthcare provider with no access to Nationwide Children’s Epic system, submission of a completed Oncology Genetic Test Requisition Form is required. If you are an internal ordering provider with access to Nationwide Children’s Epic system, no requisition form is required; please place the lab order electronically in Epic.

If submitting tissue scroll specimen, please use this Tissue Scroll Calculator tool to determine the thickness and number of tissue scrolls to send to the laboratory.

For liquid tumor, sample must contain at least 50% blasts by internal pathology review. For solid tumor, sample must contain at least 40% tumor by internal pathology review. Submission of a normal sample containing 0% tumor is recommended, but not required, to rule out germline origin of the variant. Please send frozen samples (e.g. snap-frozen tissue or OCT-embedded tissue) buried in adequate amount of dry ice and ship by overnight courier. Formalin-fixed paraffin embedded (FFPE) tissue blocks and FFPE tissue scrolls can be transported at room temperature.

For questions, please call (614) 722-5321 and ask to speak with a lab genetic counselor.

Clinical Information

This test is a targeted Sanger sequencing analysis used to confirm the presence of a specific DNA sequence change in tumor sample that was previously identified by a research testing or testing performed by another clinical laboratory. This test is only available as a CONFIRMATORY TEST and only offered to patients who have a known somatic sequence variant(s) detected by previous research or clinical testing. If patient does not have a previously detected somatic variant(s), then this test cannot be ordered and samples will be rejected.

 Please submit a copy of the previous tumor sequencing result, if available.

Days Performed

Monday through Saturday

Set Up Schedule

All tests not performed daily.

Typical Turnaround

3 weeks

Lab Area

Institute for Genomic Medicine

Methodology

DNA extraction, Polymerase chain reaction (PCR), Sanger sequencing

CPT Codes

81479

Synonyms

  • Targeted tumor mutation analysis, Targeted cancer mutation confirmation, Known tumor mutation testing, Targeted somatic mutation testing, Single site tumor mutation analysis, Tumor mutation Sanger confirmation, Targeted tumor Sanger sequencing, Confirmatory tumor mutation testing, Targeted Sanger sequencing for tumor, IGM Test
  • RT-PCR Fusion Transcript Analysis, fusion, Pediatric Solid Tumors, ARMS, PNET, Sarcoma, Ewing sarcoma, Alveolar rhabdomyosarcoma, Synovial sarcoma, Desmoplastic small round cell tumor, DSRCT, Congenital fibrosarcoma, Cellular mesoblastic nephroma, Primitive neuroectodermal tumor, Soft tissue sarcoma fusion transcript, Sarcoma RT-PCR, RT-PCR, EWSR1, FLI1, FLI-1, ERG, EWS, PAX3, FOXO1, PAX7, SYT, SSX1, SS18, SSX2, WT1, ETV6, NTRK3, Sarcoma gene fusion panel, Sarcoma translocation, IGM Test
Inpatient Requirements

Special Collection

At least 50% blasts must be present in Hematologic Cancer samples. At least 40% tumor must be present in Solid Tumor samples (based on internal pathology review). Copy of previous tumor sequencing result is REQUIRED.

 

Collect

Specimen Type Type of Container Volume of Specimen Status
Tissue (Snap-frozen) Tissue cassette 5mm x 5mm or larger Preferred
Tissue (Snap-frozen) Cryogenic tube 5mm x 5mm or larger Alternate
Bone marrow 4 mL Purple tube (EDTA) 4 mL Preferred
OCT-embedded tissue Tissue cassette 5mm x 5mm or larger Preferred
OCT-embedded tissue Cryogenic tube 5mm x 5mm or larger Alternate
Paraffin embedded tissue Paraffin block   Preferred
Tissue (Fresh) Tissue culture transport media 5mm x 5mm or larger Preferred
Tissue (Fresh) Sterile container with saline 5mm x 5mm or larger Alternate
Tissue scrolls (FFPE) Sterile container 10 scrolls at 15 microns thick Preferred
Tissue scrolls (OCT) Cryogenic tube 10 scrolls at 15 microns thick Preferred

Minimum Volume

Specimen Type Type of Container Minimum Volume
Tissue (Snap-frozen) Tissue cassette 5mm x 5mm
Tissue (Snap-frozen) Cryogenic tube 5mm x 5mm
Bone marrow 4 mL Purple tube (EDTA) 2 mL
OCT-embedded tissue Tissue cassette 5mm x 5mm
OCT-embedded tissue Cryogenic tube 5mm x 5mm
Paraffin embedded tissue Paraffin block  
Tissue (Fresh) Tissue culture transport media 5mm x 5mm
Tissue (Fresh) Sterile container with saline 5mm x 5mm
Tissue scrolls (FFPE) Sterile container 10 scrolls 15 micron thick
Tissue scrolls (OCT) Cryogenic tube 10 scrolls 15 micron thick

Inpatient Specimen Preparation

Tissue (Snap-frozen): Keep frozen
                                   Protect from heat
                                   Transport to laboratory as soon as possible
                                   Immediately place on dry ice and transport frozen
                                   Tumor sample must contain a minimum of 40% tumor

Bone marrow: Do not freeze
                       Keep at room temperature or refrigerate
                       Do not centrifuge
                       Tumor sample must contain a minimum of 50% tumor

OCT-embedded tissue: Keep frozen
                                      Protect from heat
                                      Immediately place on dry ice and transport frozen
                                      Transport to laboratory as soon as possible
                                      Tumor sample must contain a minimum of 40% tumor

Paraffin embedded tissue: Protect from heat
                                          Keep at room temperature

Tissue (Fresh): Do not add fixative
                         Do not freeze
                         Keep at room temperature or refrigerate
                         Transport to laboratory as soon as possible

Tissue scrolls (FFPE): Keep at room temperature
                                    Protect from heat
                                    Must accompany H&E slide from the same tissue block used to make FFPE scrolls

Tissue scrolls (OCT): Keep frozen
                                  Protect from heat
                                  Immediately place on dry ice and transport frozen
                                  Transport to laboratory as soon as possible
                                  Must accompany H&E slide from the same OCT-embedded tissue used to make OCT                                              tissue scrolls

Unacceptable Conditions

Inadequate tissue, Tissue degradation, Delayed or improper handling, Wrong type of specimen

Stability

Tissue (Snap-frozen): Frozen 12 month(s)

Bone marrow: Room temperature 24 hour(s)
Bone marrow: Refrigerated 72 hour(s)

OCT-embedded tissue: Frozen 12 month(s)

Paraffin embedded tissue: Room temperature 6 month(s)

Tissue (Fresh): Room temperature 24 hour(s)
Tissue (Fresh): Refrigerated 48 hour(s)

Tissue scrolls (FFPE): Room temperature 6 month(s)
Tissue scrolls (OCT): Frozen 12 month(s)

Remarks

If you are an external healthcare provider with no access to Nationwide Children’s Epic system, submission of a completed Oncology Genetic Test Requisition Form is required. If you are an internal ordering provider with access to Nationwide Children’s Epic system, no requisition form is required; please place the lab order electronically in Epic.

If submitting tissue scroll specimen, please use this Tissue Scroll Calculator tool to determine the thickness and number of tissue scrolls to send to the laboratory.

For liquid tumor, sample must contain at least 50% blasts by internal pathology review. For solid tumor, sample must contain at least 40% tumor by internal pathology review. Submission of a normal sample containing 0% tumor is recommended, but not required, to rule out germline origin of the variant. Please send frozen samples (e.g. snap-frozen tissue or OCT-embedded tissue) buried in adequate amount of dry ice and ship by overnight courier. Formalin-fixed paraffin embedded (FFPE) tissue blocks and FFPE tissue scrolls can be transported at room temperature.

For questions, please call (614) 722-5321 and ask to speak with a lab genetic counselor.

Clinical Information

This test is a targeted Sanger sequencing analysis used to confirm the presence of a specific DNA sequence change in tumor sample that was previously identified by a research testing or testing performed by another clinical laboratory. This test is only available as a CONFIRMATORY TEST and only offered to patients who have a known somatic sequence variant(s) detected by previous research or clinical testing. If patient does not have a previously detected somatic variant(s), then this test cannot be ordered and samples will be rejected.

 Please submit a copy of the previous tumor sequencing result, if available.

Days Performed

Monday through Saturday

Set Up Schedule

All tests not performed daily.

Typical Turnaround

3 weeks

CPT Codes

81479

Lab Area

Institute for Genomic Medicine

Synonyms

  • Targeted tumor mutation analysis, Targeted cancer mutation confirmation, Known tumor mutation testing, Targeted somatic mutation testing, Single site tumor mutation analysis, Tumor mutation Sanger confirmation, Targeted tumor Sanger sequencing, Confirmatory tumor mutation testing, Targeted Sanger sequencing for tumor, IGM Test
  • RT-PCR Fusion Transcript Analysis, fusion, Pediatric Solid Tumors, ARMS, PNET, Sarcoma, Ewing sarcoma, Alveolar rhabdomyosarcoma, Synovial sarcoma, Desmoplastic small round cell tumor, DSRCT, Congenital fibrosarcoma, Cellular mesoblastic nephroma, Primitive neuroectodermal tumor, Soft tissue sarcoma fusion transcript, Sarcoma RT-PCR, RT-PCR, EWSR1, FLI1, FLI-1, ERG, EWS, PAX3, FOXO1, PAX7, SYT, SSX1, SS18, SSX2, WT1, ETV6, NTRK3, Sarcoma gene fusion panel, Sarcoma translocation, IGM Test

Estimated Patient Price

< $1,000
Overview/Billing

Synonyms

  • Targeted tumor mutation analysis, Targeted cancer mutation confirmation, Known tumor mutation testing, Targeted somatic mutation testing, Single site tumor mutation analysis, Tumor mutation Sanger confirmation, Targeted tumor Sanger sequencing, Confirmatory tumor mutation testing, Targeted Sanger sequencing for tumor, IGM Test
  • RT-PCR Fusion Transcript Analysis, fusion, Pediatric Solid Tumors, ARMS, PNET, Sarcoma, Ewing sarcoma, Alveolar rhabdomyosarcoma, Synovial sarcoma, Desmoplastic small round cell tumor, DSRCT, Congenital fibrosarcoma, Cellular mesoblastic nephroma, Primitive neuroectodermal tumor, Soft tissue sarcoma fusion transcript, Sarcoma RT-PCR, RT-PCR, EWSR1, FLI1, FLI-1, ERG, EWS, PAX3, FOXO1, PAX7, SYT, SSX1, SS18, SSX2, WT1, ETV6, NTRK3, Sarcoma gene fusion panel, Sarcoma translocation, IGM Test

CPT Codes

81479
Interpretation

Clinical Information

This test is a targeted Sanger sequencing analysis used to confirm the presence of a specific DNA sequence change in tumor sample that was previously identified by a research testing or testing performed by another clinical laboratory. This test is only available as a CONFIRMATORY TEST and only offered to patients who have a known somatic sequence variant(s) detected by previous research or clinical testing. If patient does not have a previously detected somatic variant(s), then this test cannot be ordered and samples will be rejected.

 Please submit a copy of the previous tumor sequencing result, if available.

Methodology

DNA extraction, Polymerase chain reaction (PCR), Sanger sequencing
NCH Lab Only

Special Collection

At least 50% blasts must be present in Hematologic Cancer samples. At least 40% tumor must be present in Solid Tumor samples (based on internal pathology review). Copy of previous tumor sequencing result is REQUIRED.

 

Outpatient Submit with Specimen

Oncology Genetic Requisition

Collect

Specimen Type Type of Container Volume of Specimen Status
Tissue (Snap-frozen) Tissue cassette 5mm x 5mm or larger Preferred
Tissue (Snap-frozen) Cryogenic tube 5mm x 5mm or larger Alternate
Bone marrow 4 mL Purple tube (EDTA) 4 mL Preferred
OCT-embedded tissue Tissue cassette 5mm x 5mm or larger Preferred
OCT-embedded tissue Cryogenic tube 5mm x 5mm or larger Alternate
Paraffin embedded tissue Paraffin block   Preferred
Tissue (Fresh) Tissue culture transport media 5mm x 5mm or larger Preferred
Tissue (Fresh) Sterile container with saline 5mm x 5mm or larger Alternate
Tissue scrolls (FFPE) Sterile container 10 scrolls at 15 microns thick Preferred
Tissue scrolls (OCT) Cryogenic tube 10 scrolls at 15 microns thick Preferred

Minimum Volume

Specimen Type Type of Container Minimum Volume
Tissue (Snap-frozen) Tissue cassette 5mm x 5mm
Tissue (Snap-frozen) Cryogenic tube 5mm x 5mm
Bone marrow 4 mL Purple tube (EDTA) 2 mL
OCT-embedded tissue Tissue cassette 5mm x 5mm
OCT-embedded tissue Cryogenic tube 5mm x 5mm
Paraffin embedded tissue Paraffin block  
Tissue (Fresh) Tissue culture transport media 5mm x 5mm
Tissue (Fresh) Sterile container with saline 5mm x 5mm
Tissue scrolls (FFPE) Sterile container 10 scrolls 15 micron thick
Tissue scrolls (OCT) Cryogenic tube 10 scrolls 15 micron thick

Inpatient Specimen Preparation

Tissue (Snap-frozen): Keep frozen
                                   Protect from heat
                                   Transport to laboratory as soon as possible
                                   Immediately place on dry ice and transport frozen
                                   Tumor sample must contain a minimum of 40% tumor

Bone marrow: Do not freeze
                       Keep at room temperature or refrigerate
                       Do not centrifuge
                       Tumor sample must contain a minimum of 50% tumor

OCT-embedded tissue: Keep frozen
                                      Protect from heat
                                      Immediately place on dry ice and transport frozen
                                      Transport to laboratory as soon as possible
                                      Tumor sample must contain a minimum of 40% tumor

Paraffin embedded tissue: Protect from heat
                                          Keep at room temperature

Tissue (Fresh): Do not add fixative
                         Do not freeze
                         Keep at room temperature or refrigerate
                         Transport to laboratory as soon as possible

Tissue scrolls (FFPE): Keep at room temperature
                                    Protect from heat
                                    Must accompany H&E slide from the same tissue block used to make FFPE scrolls

Tissue scrolls (OCT): Keep frozen
                                  Protect from heat
                                  Immediately place on dry ice and transport frozen
                                  Transport to laboratory as soon as possible
                                  Must accompany H&E slide from the same OCT-embedded tissue used to make OCT                                              tissue scrolls

Outpatient Specimen Preparation

Tissue (Snap-frozen): Keep frozen
                                   Protect from heat
                                   Transport to laboratory as soon as possible
                                   Immediately place on dry ice and transport frozen
                                   Tumor sample must contain a minimum of 40% tumor

Bone marrow: Do not freeze
                       Keep at room temperature or refrigerate
                       Do not centrifuge
                       Tumor sample must contain a minimum of 50% tumor

OCT-embedded tissue: Keep frozen
                                      Protect from heat
                                      Immediately place on dry ice and transport frozen
                                      Transport to laboratory as soon as possible
                                      Tumor sample must contain a minimum of 40% tumor

Paraffin embedded tissue: Protect from heat
                                          Keep at room temperature

Tissue (Fresh): Do not add fixative
                         Do not freeze
                         Keep at room temperature or refrigerate
                         Transport to laboratory as soon as possible

Tissue scrolls (FFPE): Keep at room temperature
                                    Protect from heat
                                    Must accompany H&E slide from the same tissue block used to make FFPE scrolls

Tissue scrolls (OCT): Keep frozen
                                  Protect from heat
                                  Immediately place on dry ice and transport frozen
                                  Transport to laboratory as soon as possible
                                  Must accompany H&E slide from the same OCT-embedded tissue used to make OCT                                              tissue scrolls

InLab Processing

Send to Molecular Genetics Lab with all submitted paperwork. For Non-EPIC lab orders, lab staff to order GENSP in Sunqest.

Stability

Tissue (Snap-frozen): Frozen 12 month(s)

Bone marrow: Room temperature 24 hour(s)
Bone marrow: Refrigerated 72 hour(s)

OCT-embedded tissue: Frozen 12 month(s)

Paraffin embedded tissue: Room temperature 6 month(s)

Tissue (Fresh): Room temperature 24 hour(s)
Tissue (Fresh): Refrigerated 48 hour(s)

Tissue scrolls (FFPE): Room temperature 6 month(s)
Tissue scrolls (OCT): Frozen 12 month(s)

Unacceptable Conditions

Inadequate tissue, Tissue degradation, Delayed or improper handling, Wrong type of specimen

Days Performed

Monday through Saturday

Set Up Schedule

All tests not performed daily.

Typical Turnaround

3 weeks

Remarks

If you are an external healthcare provider with no access to Nationwide Children’s Epic system, submission of a completed Oncology Genetic Test Requisition Form is required. If you are an internal ordering provider with access to Nationwide Children’s Epic system, no requisition form is required; please place the lab order electronically in Epic.

If submitting tissue scroll specimen, please use this Tissue Scroll Calculator tool to determine the thickness and number of tissue scrolls to send to the laboratory.

For liquid tumor, sample must contain at least 50% blasts by internal pathology review. For solid tumor, sample must contain at least 40% tumor by internal pathology review. Submission of a normal sample containing 0% tumor is recommended, but not required, to rule out germline origin of the variant. Please send frozen samples (e.g. snap-frozen tissue or OCT-embedded tissue) buried in adequate amount of dry ice and ship by overnight courier. Formalin-fixed paraffin embedded (FFPE) tissue blocks and FFPE tissue scrolls can be transported at room temperature.

For questions, please call (614) 722-5321 and ask to speak with a lab genetic counselor.

Clinical Information

This test is a targeted Sanger sequencing analysis used to confirm the presence of a specific DNA sequence change in tumor sample that was previously identified by a research testing or testing performed by another clinical laboratory. This test is only available as a CONFIRMATORY TEST and only offered to patients who have a known somatic sequence variant(s) detected by previous research or clinical testing. If patient does not have a previously detected somatic variant(s), then this test cannot be ordered and samples will be rejected.

 Please submit a copy of the previous tumor sequencing result, if available.

Synonyms

  • Targeted tumor mutation analysis, Targeted cancer mutation confirmation, Known tumor mutation testing, Targeted somatic mutation testing, Single site tumor mutation analysis, Tumor mutation Sanger confirmation, Targeted tumor Sanger sequencing, Confirmatory tumor mutation testing, Targeted Sanger sequencing for tumor, IGM Test
  • RT-PCR Fusion Transcript Analysis, fusion, Pediatric Solid Tumors, ARMS, PNET, Sarcoma, Ewing sarcoma, Alveolar rhabdomyosarcoma, Synovial sarcoma, Desmoplastic small round cell tumor, DSRCT, Congenital fibrosarcoma, Cellular mesoblastic nephroma, Primitive neuroectodermal tumor, Soft tissue sarcoma fusion transcript, Sarcoma RT-PCR, RT-PCR, EWSR1, FLI1, FLI-1, ERG, EWS, PAX3, FOXO1, PAX7, SYT, SSX1, SS18, SSX2, WT1, ETV6, NTRK3, Sarcoma gene fusion panel, Sarcoma translocation, IGM Test

Methodology

DNA extraction, Polymerase chain reaction (PCR), Sanger sequencing

CPT Codes

81479

Estimated Patient Price

< $1,000

DC Code

5321

Downtime Availability

4-Not available

Lab Area

Lab Area
Institute for Genomic Medicine

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