Special Ordering Instructions

This test is NOT for MRD detection

Special Collection

At least 10% tumor must be present in the submitted hematologic or solid tumor sample (based on internal pathology review). This test is NOT for MRD detection. Copy of previous gene fusion result is REQUIRED.

Outpatient Submit with Specimen

Collect

Specimen Type Type of Container Volume of Specimen Status
Tissue (Snap-frozen) Tissue cassette 5mm x 5mm or larger Preferred
Tissue (Snap-frozen) Cryogenic tube 5mm x 5mm or larger Alternate
Bone marrow 4 mL Purple tube (EDTA) 4 mL Preferred
OCT-embedded tissue Tissue cassette 5mm x 5mm or larger Preferred
OCT-embedded tissue Cryogenic tube 5mm x 5mm or larger Alternate
Paraffin embedded tissue Paraffin block   Preferred
Tissue (Fresh) Tissue culture transport media 5mm x 5mm or larger Preferred
Tissue scrolls (FFPE) Sterile container   Preferred

Outpatient Specimen Preparation

Tissue (Snap-frozen): Keep frozen
                                    Protect from heat
                                    Immediately place on dry ice and transport frozen
                                    Transport to laboratory as soon as possible

Bone marrow: Do not freeze

OCT-embedded tissue: Keep frozen
                                      Protect from heat
                                      Immediately place on dry ice and transport frozen
                                      Transport to laboratory as soon as possible

Paraffin embedded tissue: Protect from heat
                                           Keep at room temperature

Tissue (Fresh): Do not add fixative
                         Transport to laboratory as soon as possible
                         Do not freeze
                    
Tissue scrolls (FFPE): Protect from heat
                                    Keep at room temperature
                                    Must accompany H&E slide from the same tissue block used to make FFPE scrolls
 

Unacceptable Conditions

Inadequate tissue, Delayed or improper handling, Tissue degradation

Stability

Tissue (Snap-frozen): Frozen 12 month(s)

Bone marrow: Room temperature 24 hour(s)

OCT-embedded tissue: Frozen 12 month(s)

Paraffin embedded tissue: Room temperature 6 month(s)

Tissue (Fresh): Room temperature 24 hour(s)

Tissue scrolls (FFPE): Room temperature 6 month(s)

Remarks

If you are an external healthcare provider with no access to Nationwide Children’s Epic system, submission of a completed Oncology Genetic Test Requisition Form is required. If you are an internal ordering provider with access to Nationwide Children’s Epic system, no requisition form is required; please place the lab order electronically in Epic. For questions, please call (614) 722-5321.

Submitted tumor sample must contain at least 10% tumor based on Nationwide Children's Laboratory internal pathology review. Please send frozen samples (e.g. snap-frozen tissue or OCT-embedded tissue) buried in adequate amount of dry ice and ship by overnight courier. Formalin-fixed paraffin-embedded (FFPE) tissue block and FFPE tissue scrolls can be transported at room temperature.

If submitting tissue scroll specimen, please use this Tissue Scroll Calculator tool to determine the thickness and number of tissue scrolls to send to the laboratory.

 

Clinical Information

This test is a targeted RT-PCR analysis used to confirm the presence of a specific gene fusion (translocation) in a tumor sample that was previously identified by a research testing or by testing performed by another clinical laboratory. If a gene fusion is detected by this RT-PCR assay, a Sanger sequencing of PCR product will be performed to confirm the presence of gene fusion in the submitted sample.

This test is only available as a CONFIRMATORY TEST and only offered to patients who have a known gene fusion detected by previous research or clinical testing. If patient does not have a previously detected gene fusion, then this test cannot be ordered and sample will be rejected.

Please submit a copy of the previous tumor fusion test result, if available.

This test is a qualitative test that can test for the presence or absence of gene fusion previously identified in the patient. This test is not a qualitative test, will not be able to report what % of the sample harbors the gene fusion, and is not a test for Minimal Residual Disease (MRD). 

Days Performed

Monday through Friday

Set Up Schedule

All tests not performed daily.

Typical Turnaround

3 weeks

Lab Area

Institute for Genomic Medicine

Methodology

Reverse transcriptase polymerase chain reaction (RT-PCR), Sanger sequencing

CPT Codes

81479

Synonyms

  • Cancer gene fusion confirmation, Tumor gene fusion confirmation, Cancer translocation confirmation, Tumor translocation confirmation, Somatic gene fusion confirmation, RNA fusion confirmation, Confirmatory tumor fusion testing, Targeted gene fusion confirmation, Targeted RT-PCR, IGM Test

Special Ordering Instructions

This test is NOT for MRD detection

Special Collection

At least 10% tumor must be present in the submitted hematologic or solid tumor sample (based on internal pathology review). This test is NOT for MRD detection. Copy of previous gene fusion result is REQUIRED.

Collect

Specimen Type Type of Container Volume of Specimen Status
Tissue (Snap-frozen) Tissue cassette 5mm x 5mm or larger Preferred
Tissue (Snap-frozen) Cryogenic tube 5mm x 5mm or larger Alternate
Bone marrow 4 mL Purple tube (EDTA) 4 mL Preferred
OCT-embedded tissue Tissue cassette 5mm x 5mm or larger Preferred
OCT-embedded tissue Cryogenic tube 5mm x 5mm or larger Alternate
Paraffin embedded tissue Paraffin block   Preferred
Tissue (Fresh) Tissue culture transport media 5mm x 5mm or larger Preferred
Tissue scrolls (FFPE) Sterile container   Preferred

Minimum Volume

Specimen Type Type of Container Minimum Volume
Tissue (Snap-frozen) Tissue cassette 5mm x 5mm
Tissue (Snap-frozen) Cryogenic tube 5mm x 5mm
Bone marrow 4 mL Purple tube (EDTA) 3 mL
OCT-embedded tissue Tissue cassette 5mm x 5mm
OCT-embedded tissue Cryogenic tube 5mm x 5mm
Paraffin embedded tissue Paraffin block  
Tissue (Fresh) Tissue culture transport media 5mm x 5mm
Tissue scrolls (FFPE) Sterile container  

Inpatient Specimen Preparation

Tissue (Snap-frozen): Keep frozen
                                    Protect from heat
                                    Immediately place on dry ice and transport frozen
                                    Transport to laboratory as soon as possible

Bone marrow: Do not freeze

OCT-embedded tissue: Keep frozen
                                      Protect from heat
                                      Immediately place on dry ice and transport frozen
                                      Transport to laboratory as soon as possible

Paraffin embedded tissue: Protect from heat
                                           Keep at room temperature

Tissue (Fresh): Do not add fixative
                         Transport to laboratory as soon as possible
                         Do not freeze
                    
Tissue scrolls (FFPE): Protect from heat
                                    Keep at room temperature
                                    Must accompany H&E slide from the same tissue block used to make FFPE scrolls

Unacceptable Conditions

Inadequate tissue, Delayed or improper handling, Tissue degradation

Stability

Tissue (Snap-frozen): Frozen 12 month(s)

Bone marrow: Room temperature 24 hour(s)

OCT-embedded tissue: Frozen 12 month(s)

Paraffin embedded tissue: Room temperature 6 month(s)

Tissue (Fresh): Room temperature 24 hour(s)

Tissue scrolls (FFPE): Room temperature 6 month(s)

Remarks

If you are an external healthcare provider with no access to Nationwide Children’s Epic system, submission of a completed Oncology Genetic Test Requisition Form is required. If you are an internal ordering provider with access to Nationwide Children’s Epic system, no requisition form is required; please place the lab order electronically in Epic. For questions, please call (614) 722-5321.

Submitted tumor sample must contain at least 10% tumor based on Nationwide Children's Laboratory internal pathology review. Please send frozen samples (e.g. snap-frozen tissue or OCT-embedded tissue) buried in adequate amount of dry ice and ship by overnight courier. Formalin-fixed paraffin-embedded (FFPE) tissue block and FFPE tissue scrolls can be transported at room temperature.

If submitting tissue scroll specimen, please use this Tissue Scroll Calculator tool to determine the thickness and number of tissue scrolls to send to the laboratory.

 

Clinical Information

This test is a targeted RT-PCR analysis used to confirm the presence of a specific gene fusion (translocation) in a tumor sample that was previously identified by a research testing or by testing performed by another clinical laboratory. If a gene fusion is detected by this RT-PCR assay, a Sanger sequencing of PCR product will be performed to confirm the presence of gene fusion in the submitted sample.

This test is only available as a CONFIRMATORY TEST and only offered to patients who have a known gene fusion detected by previous research or clinical testing. If patient does not have a previously detected gene fusion, then this test cannot be ordered and sample will be rejected.

Please submit a copy of the previous tumor fusion test result, if available.

This test is a qualitative test that can test for the presence or absence of gene fusion previously identified in the patient. This test is not a qualitative test, will not be able to report what % of the sample harbors the gene fusion, and is not a test for Minimal Residual Disease (MRD). 

Days Performed

Monday through Friday

Set Up Schedule

All tests not performed daily.

Typical Turnaround

3 weeks

CPT Codes

81479

Lab Area

Institute for Genomic Medicine

Synonyms

  • Cancer gene fusion confirmation, Tumor gene fusion confirmation, Cancer translocation confirmation, Tumor translocation confirmation, Somatic gene fusion confirmation, RNA fusion confirmation, Confirmatory tumor fusion testing, Targeted gene fusion confirmation, Targeted RT-PCR, IGM Test

Estimated Patient Price

< $1,000

Synonyms

  • Cancer gene fusion confirmation, Tumor gene fusion confirmation, Cancer translocation confirmation, Tumor translocation confirmation, Somatic gene fusion confirmation, RNA fusion confirmation, Confirmatory tumor fusion testing, Targeted gene fusion confirmation, Targeted RT-PCR, IGM Test

CPT Codes

81479

Clinical Information

This test is a targeted RT-PCR analysis used to confirm the presence of a specific gene fusion (translocation) in a tumor sample that was previously identified by a research testing or by testing performed by another clinical laboratory. If a gene fusion is detected by this RT-PCR assay, a Sanger sequencing of PCR product will be performed to confirm the presence of gene fusion in the submitted sample.

This test is only available as a CONFIRMATORY TEST and only offered to patients who have a known gene fusion detected by previous research or clinical testing. If patient does not have a previously detected gene fusion, then this test cannot be ordered and sample will be rejected.

Please submit a copy of the previous tumor fusion test result, if available.

This test is a qualitative test that can test for the presence or absence of gene fusion previously identified in the patient. This test is not a qualitative test, will not be able to report what % of the sample harbors the gene fusion, and is not a test for Minimal Residual Disease (MRD). 

Methodology

Reverse transcriptase polymerase chain reaction (RT-PCR), Sanger sequencing

Special Ordering Instructions

This test is NOT for MRD detection

Special Collection

At least 10% tumor must be present in the submitted hematologic or solid tumor sample (based on internal pathology review). This test is NOT for MRD detection. Copy of previous gene fusion result is REQUIRED.

Outpatient Submit with Specimen

Collect

Specimen Type Type of Container Volume of Specimen Status
Tissue (Snap-frozen) Tissue cassette 5mm x 5mm or larger Preferred
Tissue (Snap-frozen) Cryogenic tube 5mm x 5mm or larger Alternate
Bone marrow 4 mL Purple tube (EDTA) 4 mL Preferred
OCT-embedded tissue Tissue cassette 5mm x 5mm or larger Preferred
OCT-embedded tissue Cryogenic tube 5mm x 5mm or larger Alternate
Paraffin embedded tissue Paraffin block   Preferred
Tissue (Fresh) Tissue culture transport media 5mm x 5mm or larger Preferred
Tissue scrolls (FFPE) Sterile container   Preferred

Minimum Volume

Specimen Type Type of Container Minimum Volume
Tissue (Snap-frozen) Tissue cassette 5mm x 5mm
Tissue (Snap-frozen) Cryogenic tube 5mm x 5mm
Bone marrow 4 mL Purple tube (EDTA) 3 mL
OCT-embedded tissue Tissue cassette 5mm x 5mm
OCT-embedded tissue Cryogenic tube 5mm x 5mm
Paraffin embedded tissue Paraffin block  
Tissue (Fresh) Tissue culture transport media 5mm x 5mm
Tissue scrolls (FFPE) Sterile container  

Inpatient Specimen Preparation

Tissue (Snap-frozen): Keep frozen
                                    Protect from heat
                                    Immediately place on dry ice and transport frozen
                                    Transport to laboratory as soon as possible

Bone marrow: Do not freeze

OCT-embedded tissue: Keep frozen
                                      Protect from heat
                                      Immediately place on dry ice and transport frozen
                                      Transport to laboratory as soon as possible

Paraffin embedded tissue: Protect from heat
                                           Keep at room temperature

Tissue (Fresh): Do not add fixative
                         Transport to laboratory as soon as possible
                         Do not freeze
                    
Tissue scrolls (FFPE): Protect from heat
                                    Keep at room temperature
                                    Must accompany H&E slide from the same tissue block used to make FFPE scrolls

Outpatient Specimen Preparation

Tissue (Snap-frozen): Keep frozen
                                    Protect from heat
                                    Immediately place on dry ice and transport frozen
                                    Transport to laboratory as soon as possible

Bone marrow: Do not freeze

OCT-embedded tissue: Keep frozen
                                      Protect from heat
                                      Immediately place on dry ice and transport frozen
                                      Transport to laboratory as soon as possible

Paraffin embedded tissue: Protect from heat
                                           Keep at room temperature

Tissue (Fresh): Do not add fixative
                         Transport to laboratory as soon as possible
                         Do not freeze
                    
Tissue scrolls (FFPE): Protect from heat
                                    Keep at room temperature
                                    Must accompany H&E slide from the same tissue block used to make FFPE scrolls
 

InLab Processing

TIME SENSITIVE SPECIMEN. Send to Molecular Genetics Lab immediately with all submitted paperwork. For Non-EPIC lab orders, NCH lab staff to order GENSP in Sunqest.

Stability

Tissue (Snap-frozen): Frozen 12 month(s)

Bone marrow: Room temperature 24 hour(s)

OCT-embedded tissue: Frozen 12 month(s)

Paraffin embedded tissue: Room temperature 6 month(s)

Tissue (Fresh): Room temperature 24 hour(s)

Tissue scrolls (FFPE): Room temperature 6 month(s)

Unacceptable Conditions

Inadequate tissue, Delayed or improper handling, Tissue degradation

Days Performed

Monday through Friday

Set Up Schedule

All tests not performed daily.

Typical Turnaround

3 weeks

Remarks

If you are an external healthcare provider with no access to Nationwide Children’s Epic system, submission of a completed Oncology Genetic Test Requisition Form is required. If you are an internal ordering provider with access to Nationwide Children’s Epic system, no requisition form is required; please place the lab order electronically in Epic. For questions, please call (614) 722-5321.

Submitted tumor sample must contain at least 10% tumor based on Nationwide Children's Laboratory internal pathology review. Please send frozen samples (e.g. snap-frozen tissue or OCT-embedded tissue) buried in adequate amount of dry ice and ship by overnight courier. Formalin-fixed paraffin-embedded (FFPE) tissue block and FFPE tissue scrolls can be transported at room temperature.

If submitting tissue scroll specimen, please use this Tissue Scroll Calculator tool to determine the thickness and number of tissue scrolls to send to the laboratory.

 

Clinical Information

This test is a targeted RT-PCR analysis used to confirm the presence of a specific gene fusion (translocation) in a tumor sample that was previously identified by a research testing or by testing performed by another clinical laboratory. If a gene fusion is detected by this RT-PCR assay, a Sanger sequencing of PCR product will be performed to confirm the presence of gene fusion in the submitted sample.

This test is only available as a CONFIRMATORY TEST and only offered to patients who have a known gene fusion detected by previous research or clinical testing. If patient does not have a previously detected gene fusion, then this test cannot be ordered and sample will be rejected.

Please submit a copy of the previous tumor fusion test result, if available.

This test is a qualitative test that can test for the presence or absence of gene fusion previously identified in the patient. This test is not a qualitative test, will not be able to report what % of the sample harbors the gene fusion, and is not a test for Minimal Residual Disease (MRD). 

Synonyms

  • Cancer gene fusion confirmation, Tumor gene fusion confirmation, Cancer translocation confirmation, Tumor translocation confirmation, Somatic gene fusion confirmation, RNA fusion confirmation, Confirmatory tumor fusion testing, Targeted gene fusion confirmation, Targeted RT-PCR, IGM Test

Methodology

Reverse transcriptase polymerase chain reaction (RT-PCR), Sanger sequencing

CPT Codes

81479

Estimated Patient Price

< $1,000

DC Code

5321

Downtime Availability

4-Not available
Outpatient Requirements

Special Ordering Instructions

This test is NOT for MRD detection

Special Collection

At least 10% tumor must be present in the submitted hematologic or solid tumor sample (based on internal pathology review). This test is NOT for MRD detection. Copy of previous gene fusion result is REQUIRED.

Outpatient Submit with Specimen

Collect

Specimen Type Type of Container Volume of Specimen Status
Tissue (Snap-frozen) Tissue cassette 5mm x 5mm or larger Preferred
Tissue (Snap-frozen) Cryogenic tube 5mm x 5mm or larger Alternate
Bone marrow 4 mL Purple tube (EDTA) 4 mL Preferred
OCT-embedded tissue Tissue cassette 5mm x 5mm or larger Preferred
OCT-embedded tissue Cryogenic tube 5mm x 5mm or larger Alternate
Paraffin embedded tissue Paraffin block   Preferred
Tissue (Fresh) Tissue culture transport media 5mm x 5mm or larger Preferred
Tissue scrolls (FFPE) Sterile container   Preferred

Outpatient Specimen Preparation

Tissue (Snap-frozen): Keep frozen
                                    Protect from heat
                                    Immediately place on dry ice and transport frozen
                                    Transport to laboratory as soon as possible

Bone marrow: Do not freeze

OCT-embedded tissue: Keep frozen
                                      Protect from heat
                                      Immediately place on dry ice and transport frozen
                                      Transport to laboratory as soon as possible

Paraffin embedded tissue: Protect from heat
                                           Keep at room temperature

Tissue (Fresh): Do not add fixative
                         Transport to laboratory as soon as possible
                         Do not freeze
                    
Tissue scrolls (FFPE): Protect from heat
                                    Keep at room temperature
                                    Must accompany H&E slide from the same tissue block used to make FFPE scrolls
 

Unacceptable Conditions

Inadequate tissue, Delayed or improper handling, Tissue degradation

Stability

Tissue (Snap-frozen): Frozen 12 month(s)

Bone marrow: Room temperature 24 hour(s)

OCT-embedded tissue: Frozen 12 month(s)

Paraffin embedded tissue: Room temperature 6 month(s)

Tissue (Fresh): Room temperature 24 hour(s)

Tissue scrolls (FFPE): Room temperature 6 month(s)

Remarks

If you are an external healthcare provider with no access to Nationwide Children’s Epic system, submission of a completed Oncology Genetic Test Requisition Form is required. If you are an internal ordering provider with access to Nationwide Children’s Epic system, no requisition form is required; please place the lab order electronically in Epic. For questions, please call (614) 722-5321.

Submitted tumor sample must contain at least 10% tumor based on Nationwide Children's Laboratory internal pathology review. Please send frozen samples (e.g. snap-frozen tissue or OCT-embedded tissue) buried in adequate amount of dry ice and ship by overnight courier. Formalin-fixed paraffin-embedded (FFPE) tissue block and FFPE tissue scrolls can be transported at room temperature.

If submitting tissue scroll specimen, please use this Tissue Scroll Calculator tool to determine the thickness and number of tissue scrolls to send to the laboratory.

 

Clinical Information

This test is a targeted RT-PCR analysis used to confirm the presence of a specific gene fusion (translocation) in a tumor sample that was previously identified by a research testing or by testing performed by another clinical laboratory. If a gene fusion is detected by this RT-PCR assay, a Sanger sequencing of PCR product will be performed to confirm the presence of gene fusion in the submitted sample.

This test is only available as a CONFIRMATORY TEST and only offered to patients who have a known gene fusion detected by previous research or clinical testing. If patient does not have a previously detected gene fusion, then this test cannot be ordered and sample will be rejected.

Please submit a copy of the previous tumor fusion test result, if available.

This test is a qualitative test that can test for the presence or absence of gene fusion previously identified in the patient. This test is not a qualitative test, will not be able to report what % of the sample harbors the gene fusion, and is not a test for Minimal Residual Disease (MRD). 

Days Performed

Monday through Friday

Set Up Schedule

All tests not performed daily.

Typical Turnaround

3 weeks

Lab Area

Institute for Genomic Medicine

Methodology

Reverse transcriptase polymerase chain reaction (RT-PCR), Sanger sequencing

CPT Codes

81479

Synonyms

  • Cancer gene fusion confirmation, Tumor gene fusion confirmation, Cancer translocation confirmation, Tumor translocation confirmation, Somatic gene fusion confirmation, RNA fusion confirmation, Confirmatory tumor fusion testing, Targeted gene fusion confirmation, Targeted RT-PCR, IGM Test
Inpatient Requirements

Special Ordering Instructions

This test is NOT for MRD detection

Special Collection

At least 10% tumor must be present in the submitted hematologic or solid tumor sample (based on internal pathology review). This test is NOT for MRD detection. Copy of previous gene fusion result is REQUIRED.

Collect

Specimen Type Type of Container Volume of Specimen Status
Tissue (Snap-frozen) Tissue cassette 5mm x 5mm or larger Preferred
Tissue (Snap-frozen) Cryogenic tube 5mm x 5mm or larger Alternate
Bone marrow 4 mL Purple tube (EDTA) 4 mL Preferred
OCT-embedded tissue Tissue cassette 5mm x 5mm or larger Preferred
OCT-embedded tissue Cryogenic tube 5mm x 5mm or larger Alternate
Paraffin embedded tissue Paraffin block   Preferred
Tissue (Fresh) Tissue culture transport media 5mm x 5mm or larger Preferred
Tissue scrolls (FFPE) Sterile container   Preferred

Minimum Volume

Specimen Type Type of Container Minimum Volume
Tissue (Snap-frozen) Tissue cassette 5mm x 5mm
Tissue (Snap-frozen) Cryogenic tube 5mm x 5mm
Bone marrow 4 mL Purple tube (EDTA) 3 mL
OCT-embedded tissue Tissue cassette 5mm x 5mm
OCT-embedded tissue Cryogenic tube 5mm x 5mm
Paraffin embedded tissue Paraffin block  
Tissue (Fresh) Tissue culture transport media 5mm x 5mm
Tissue scrolls (FFPE) Sterile container  

Inpatient Specimen Preparation

Tissue (Snap-frozen): Keep frozen
                                    Protect from heat
                                    Immediately place on dry ice and transport frozen
                                    Transport to laboratory as soon as possible

Bone marrow: Do not freeze

OCT-embedded tissue: Keep frozen
                                      Protect from heat
                                      Immediately place on dry ice and transport frozen
                                      Transport to laboratory as soon as possible

Paraffin embedded tissue: Protect from heat
                                           Keep at room temperature

Tissue (Fresh): Do not add fixative
                         Transport to laboratory as soon as possible
                         Do not freeze
                    
Tissue scrolls (FFPE): Protect from heat
                                    Keep at room temperature
                                    Must accompany H&E slide from the same tissue block used to make FFPE scrolls

Unacceptable Conditions

Inadequate tissue, Delayed or improper handling, Tissue degradation

Stability

Tissue (Snap-frozen): Frozen 12 month(s)

Bone marrow: Room temperature 24 hour(s)

OCT-embedded tissue: Frozen 12 month(s)

Paraffin embedded tissue: Room temperature 6 month(s)

Tissue (Fresh): Room temperature 24 hour(s)

Tissue scrolls (FFPE): Room temperature 6 month(s)

Remarks

If you are an external healthcare provider with no access to Nationwide Children’s Epic system, submission of a completed Oncology Genetic Test Requisition Form is required. If you are an internal ordering provider with access to Nationwide Children’s Epic system, no requisition form is required; please place the lab order electronically in Epic. For questions, please call (614) 722-5321.

Submitted tumor sample must contain at least 10% tumor based on Nationwide Children's Laboratory internal pathology review. Please send frozen samples (e.g. snap-frozen tissue or OCT-embedded tissue) buried in adequate amount of dry ice and ship by overnight courier. Formalin-fixed paraffin-embedded (FFPE) tissue block and FFPE tissue scrolls can be transported at room temperature.

If submitting tissue scroll specimen, please use this Tissue Scroll Calculator tool to determine the thickness and number of tissue scrolls to send to the laboratory.

 

Clinical Information

This test is a targeted RT-PCR analysis used to confirm the presence of a specific gene fusion (translocation) in a tumor sample that was previously identified by a research testing or by testing performed by another clinical laboratory. If a gene fusion is detected by this RT-PCR assay, a Sanger sequencing of PCR product will be performed to confirm the presence of gene fusion in the submitted sample.

This test is only available as a CONFIRMATORY TEST and only offered to patients who have a known gene fusion detected by previous research or clinical testing. If patient does not have a previously detected gene fusion, then this test cannot be ordered and sample will be rejected.

Please submit a copy of the previous tumor fusion test result, if available.

This test is a qualitative test that can test for the presence or absence of gene fusion previously identified in the patient. This test is not a qualitative test, will not be able to report what % of the sample harbors the gene fusion, and is not a test for Minimal Residual Disease (MRD). 

Days Performed

Monday through Friday

Set Up Schedule

All tests not performed daily.

Typical Turnaround

3 weeks

CPT Codes

81479

Lab Area

Institute for Genomic Medicine

Synonyms

  • Cancer gene fusion confirmation, Tumor gene fusion confirmation, Cancer translocation confirmation, Tumor translocation confirmation, Somatic gene fusion confirmation, RNA fusion confirmation, Confirmatory tumor fusion testing, Targeted gene fusion confirmation, Targeted RT-PCR, IGM Test

Estimated Patient Price

< $1,000
Overview/Billing

Synonyms

  • Cancer gene fusion confirmation, Tumor gene fusion confirmation, Cancer translocation confirmation, Tumor translocation confirmation, Somatic gene fusion confirmation, RNA fusion confirmation, Confirmatory tumor fusion testing, Targeted gene fusion confirmation, Targeted RT-PCR, IGM Test

CPT Codes

81479
Interpretation

Clinical Information

This test is a targeted RT-PCR analysis used to confirm the presence of a specific gene fusion (translocation) in a tumor sample that was previously identified by a research testing or by testing performed by another clinical laboratory. If a gene fusion is detected by this RT-PCR assay, a Sanger sequencing of PCR product will be performed to confirm the presence of gene fusion in the submitted sample.

This test is only available as a CONFIRMATORY TEST and only offered to patients who have a known gene fusion detected by previous research or clinical testing. If patient does not have a previously detected gene fusion, then this test cannot be ordered and sample will be rejected.

Please submit a copy of the previous tumor fusion test result, if available.

This test is a qualitative test that can test for the presence or absence of gene fusion previously identified in the patient. This test is not a qualitative test, will not be able to report what % of the sample harbors the gene fusion, and is not a test for Minimal Residual Disease (MRD). 

Methodology

Reverse transcriptase polymerase chain reaction (RT-PCR), Sanger sequencing
NCH Lab Only

Special Ordering Instructions

This test is NOT for MRD detection

Special Collection

At least 10% tumor must be present in the submitted hematologic or solid tumor sample (based on internal pathology review). This test is NOT for MRD detection. Copy of previous gene fusion result is REQUIRED.

Outpatient Submit with Specimen

Collect

Specimen Type Type of Container Volume of Specimen Status
Tissue (Snap-frozen) Tissue cassette 5mm x 5mm or larger Preferred
Tissue (Snap-frozen) Cryogenic tube 5mm x 5mm or larger Alternate
Bone marrow 4 mL Purple tube (EDTA) 4 mL Preferred
OCT-embedded tissue Tissue cassette 5mm x 5mm or larger Preferred
OCT-embedded tissue Cryogenic tube 5mm x 5mm or larger Alternate
Paraffin embedded tissue Paraffin block   Preferred
Tissue (Fresh) Tissue culture transport media 5mm x 5mm or larger Preferred
Tissue scrolls (FFPE) Sterile container   Preferred

Minimum Volume

Specimen Type Type of Container Minimum Volume
Tissue (Snap-frozen) Tissue cassette 5mm x 5mm
Tissue (Snap-frozen) Cryogenic tube 5mm x 5mm
Bone marrow 4 mL Purple tube (EDTA) 3 mL
OCT-embedded tissue Tissue cassette 5mm x 5mm
OCT-embedded tissue Cryogenic tube 5mm x 5mm
Paraffin embedded tissue Paraffin block  
Tissue (Fresh) Tissue culture transport media 5mm x 5mm
Tissue scrolls (FFPE) Sterile container  

Inpatient Specimen Preparation

Tissue (Snap-frozen): Keep frozen
                                    Protect from heat
                                    Immediately place on dry ice and transport frozen
                                    Transport to laboratory as soon as possible

Bone marrow: Do not freeze

OCT-embedded tissue: Keep frozen
                                      Protect from heat
                                      Immediately place on dry ice and transport frozen
                                      Transport to laboratory as soon as possible

Paraffin embedded tissue: Protect from heat
                                           Keep at room temperature

Tissue (Fresh): Do not add fixative
                         Transport to laboratory as soon as possible
                         Do not freeze
                    
Tissue scrolls (FFPE): Protect from heat
                                    Keep at room temperature
                                    Must accompany H&E slide from the same tissue block used to make FFPE scrolls

Outpatient Specimen Preparation

Tissue (Snap-frozen): Keep frozen
                                    Protect from heat
                                    Immediately place on dry ice and transport frozen
                                    Transport to laboratory as soon as possible

Bone marrow: Do not freeze

OCT-embedded tissue: Keep frozen
                                      Protect from heat
                                      Immediately place on dry ice and transport frozen
                                      Transport to laboratory as soon as possible

Paraffin embedded tissue: Protect from heat
                                           Keep at room temperature

Tissue (Fresh): Do not add fixative
                         Transport to laboratory as soon as possible
                         Do not freeze
                    
Tissue scrolls (FFPE): Protect from heat
                                    Keep at room temperature
                                    Must accompany H&E slide from the same tissue block used to make FFPE scrolls
 

InLab Processing

TIME SENSITIVE SPECIMEN. Send to Molecular Genetics Lab immediately with all submitted paperwork. For Non-EPIC lab orders, NCH lab staff to order GENSP in Sunqest.

Stability

Tissue (Snap-frozen): Frozen 12 month(s)

Bone marrow: Room temperature 24 hour(s)

OCT-embedded tissue: Frozen 12 month(s)

Paraffin embedded tissue: Room temperature 6 month(s)

Tissue (Fresh): Room temperature 24 hour(s)

Tissue scrolls (FFPE): Room temperature 6 month(s)

Unacceptable Conditions

Inadequate tissue, Delayed or improper handling, Tissue degradation

Days Performed

Monday through Friday

Set Up Schedule

All tests not performed daily.

Typical Turnaround

3 weeks

Remarks

If you are an external healthcare provider with no access to Nationwide Children’s Epic system, submission of a completed Oncology Genetic Test Requisition Form is required. If you are an internal ordering provider with access to Nationwide Children’s Epic system, no requisition form is required; please place the lab order electronically in Epic. For questions, please call (614) 722-5321.

Submitted tumor sample must contain at least 10% tumor based on Nationwide Children's Laboratory internal pathology review. Please send frozen samples (e.g. snap-frozen tissue or OCT-embedded tissue) buried in adequate amount of dry ice and ship by overnight courier. Formalin-fixed paraffin-embedded (FFPE) tissue block and FFPE tissue scrolls can be transported at room temperature.

If submitting tissue scroll specimen, please use this Tissue Scroll Calculator tool to determine the thickness and number of tissue scrolls to send to the laboratory.

 

Clinical Information

This test is a targeted RT-PCR analysis used to confirm the presence of a specific gene fusion (translocation) in a tumor sample that was previously identified by a research testing or by testing performed by another clinical laboratory. If a gene fusion is detected by this RT-PCR assay, a Sanger sequencing of PCR product will be performed to confirm the presence of gene fusion in the submitted sample.

This test is only available as a CONFIRMATORY TEST and only offered to patients who have a known gene fusion detected by previous research or clinical testing. If patient does not have a previously detected gene fusion, then this test cannot be ordered and sample will be rejected.

Please submit a copy of the previous tumor fusion test result, if available.

This test is a qualitative test that can test for the presence or absence of gene fusion previously identified in the patient. This test is not a qualitative test, will not be able to report what % of the sample harbors the gene fusion, and is not a test for Minimal Residual Disease (MRD). 

Synonyms

  • Cancer gene fusion confirmation, Tumor gene fusion confirmation, Cancer translocation confirmation, Tumor translocation confirmation, Somatic gene fusion confirmation, RNA fusion confirmation, Confirmatory tumor fusion testing, Targeted gene fusion confirmation, Targeted RT-PCR, IGM Test

Methodology

Reverse transcriptase polymerase chain reaction (RT-PCR), Sanger sequencing

CPT Codes

81479

Estimated Patient Price

< $1,000

DC Code

5321

Downtime Availability

4-Not available

Lab Area

Lab Area
Institute for Genomic Medicine