Special Collection

FFPE sample is NOT accepted for this test.

Outpatient Submit with Specimen

Oncology Genetic Requisition

Collect

Specimen Type Type of Container Volume of Specimen Status
Tissue (Snap-frozen) Tissue cassette   Preferred
Tissue (Snap-frozen) Cryogenic tube   Alternate
OCT-embedded tissue Tissue cassette   Preferred
OCT-embedded tissue Cryogenic tube   Alternate

Outpatient Specimen Preparation

Tissue (Snap-frozen): Keep frozen
                                    Do not add fixative
                                    Immediately place on dry ice and transport frozen
                                    Transport to laboratory as soon as possible
                                    Protect from heat

OCT-embedded tissue: Keep frozen
                                      Transport to laboratory as soon as possible
                                      Protect from heat
                                      Immediately place on dry ice and transport frozen
 

Unacceptable Conditions

Delayed or improper handling, Tissue degradation, Formalin Fixed Tissue, Thawed frozen specimen, Wrong type of specimen

Stability

Tissue (Snap-frozen): Frozen 6 month(s)

OCT-embedded tissue: Frozen 6 month(s)
 

Remarks

If you are an external healthcare provider with no access to Nationwide Children’s Epic system, submission of a completed Oncology Genetic Test Requisition Form is required;. If you are an internal ordering provider with access to Nationwide Children’s Epic system, no requisition form is required; please place the lab order electronically in Epic.

Only snap-frozen or OCT-embedded tissue is accepted for this testing. Paraffin-embedded tissue is NOT accepted for this testing. Handle samples using RNA precautions and transport on dry ice. Please send any available pathology reports, even if preliminary. Ship all samples frozen via overnight delivery. Saturday deliveries are accepted. Please check "Saturday Delivery" on shipment label. Please call (614) 722-5321 with questions.

Clinical Information

This test detects specific gene fusions associated with the following sarcomas listed below.

  • Ewing sarcoma(EWS) / Primitive neuroectodermal tumors (PNET) ..... CPT codes 81401 (x2):
    • EWSR1-FLI1 (also known as EWS-FLI-1) fusion [t(11;22)(q24;q12)] and
    • EWSR1-ERG (also known as EWS-ERG) fusion [t(21;22)(q22;q12)]
  • Alveolar rhabdomyosarcoma (ARMS) ..... CPT codes 81401 (x2):
    • PAX3-FOXO1 fusion [t(2;13)(q35;q14)] and
    • PAX7-FOXO1 fusion [t(1;13)(p36;q14)]
  • Synovial sarcoma (SS) ..... CPT codes 81401 (x2):
    • SS18-SSX1 (also known as SYT-SSX1) fusion [t(X;18)(p11.2;q11.2)] and
    • SS18-SSX2 (also known as SYT-SSX2) fusion [t(X;18)(p11.2;q11.2)]
  • Desmoplastic small round cell tumor (DSRC) ..... CPT code 81401 (x1):
    • EWSR1-WT1 (also known as EWS-WT1) fusion [t(11;22)(p13;q12)]
  • Congenital fibrosarcoma/Cellular mesoblastic nephroma (CMN) ..... CPT code 81193 (x1):
    • ETV6-NTRK3 fusion [t(12;15)(p13;q25)]

Testing for one or more specific sarcoma types is available, as well as for all sarcoma types (Full Panel) -- Please specify on the Requisition Form. Fusion partners are confirmed by DNA sequence analysis of the RT-PCR product.

Days Performed

Monday through Friday

Set Up Schedule

All tests not performed daily.

Typical Turnaround

7 days

Lab Area

Institute for Genomic Medicine

Methodology

Reverse transcriptase polymerase chain reaction (RT-PCR), DNA sequencing

CPT Codes

81401 (x7), 81193

Synonyms

  • RT-PCR Fusion Transcript Analysis, fusion, Pediatric Solid Tumors, ARMS, PNET, Sarcoma, Ewing sarcoma, Alveolar rhabdomyosarcoma, Synovial sarcoma, Desmoplastic small round cell tumor, DSRCT, Congenital fibrosarcoma, Cellular mesoblastic nephroma, Primitive neuroectodermal tumor, Soft tissue sarcoma fusion transcript, Sarcoma RT-PCR, RT-PCR, EWSR1, FLI1, FLI-1, ERG, EWS, PAX3, FOXO1, PAX7, SYT, SSX1, SS18, SSX2, WT1, ETV6, NTRK3, Sarcoma gene fusion panel, Sarcoma translocation, IGM Test

Special Collection

FFPE sample is NOT accepted for this test.

Collect

Specimen Type Type of Container Volume of Specimen Status
Tissue (Snap-frozen) Tissue cassette   Preferred
Tissue (Snap-frozen) Cryogenic tube   Alternate
OCT-embedded tissue Tissue cassette   Preferred
OCT-embedded tissue Cryogenic tube   Alternate

Minimum Volume

Specimen Type Type of Container Minimum Volume
Tissue (Snap-frozen) Tissue cassette  
Tissue (Snap-frozen) Cryogenic tube  
OCT-embedded tissue Tissue cassette  
OCT-embedded tissue Cryogenic tube  

Inpatient Specimen Preparation

Tissue (Snap-frozen): Keep frozen
                                    Do not add fixative
                                    Immediately place on dry ice and transport frozen
                                    Transport to laboratory as soon as possible
                                    Protect from heat

OCT-embedded tissue: Keep frozen
                                      Transport to laboratory as soon as possible
                                      Protect from heat
                                      Immediately place on dry ice and transport frozen

Unacceptable Conditions

Delayed or improper handling, Tissue degradation, Formalin Fixed Tissue, Thawed frozen specimen, Wrong type of specimen

Stability

Tissue (Snap-frozen): Frozen 6 month(s)

OCT-embedded tissue: Frozen 6 month(s)
 

Remarks

If you are an external healthcare provider with no access to Nationwide Children’s Epic system, submission of a completed Oncology Genetic Test Requisition Form is required;. If you are an internal ordering provider with access to Nationwide Children’s Epic system, no requisition form is required; please place the lab order electronically in Epic.

Only snap-frozen or OCT-embedded tissue is accepted for this testing. Paraffin-embedded tissue is NOT accepted for this testing. Handle samples using RNA precautions and transport on dry ice. Please send any available pathology reports, even if preliminary. Ship all samples frozen via overnight delivery. Saturday deliveries are accepted. Please check "Saturday Delivery" on shipment label. Please call (614) 722-5321 with questions.

Clinical Information

This test detects specific gene fusions associated with the following sarcomas listed below.

  • Ewing sarcoma(EWS) / Primitive neuroectodermal tumors (PNET) ..... CPT codes 81401 (x2):
    • EWSR1-FLI1 (also known as EWS-FLI-1) fusion [t(11;22)(q24;q12)] and
    • EWSR1-ERG (also known as EWS-ERG) fusion [t(21;22)(q22;q12)]
  • Alveolar rhabdomyosarcoma (ARMS) ..... CPT codes 81401 (x2):
    • PAX3-FOXO1 fusion [t(2;13)(q35;q14)] and
    • PAX7-FOXO1 fusion [t(1;13)(p36;q14)]
  • Synovial sarcoma (SS) ..... CPT codes 81401 (x2):
    • SS18-SSX1 (also known as SYT-SSX1) fusion [t(X;18)(p11.2;q11.2)] and
    • SS18-SSX2 (also known as SYT-SSX2) fusion [t(X;18)(p11.2;q11.2)]
  • Desmoplastic small round cell tumor (DSRC) ..... CPT code 81401 (x1):
    • EWSR1-WT1 (also known as EWS-WT1) fusion [t(11;22)(p13;q12)]
  • Congenital fibrosarcoma/Cellular mesoblastic nephroma (CMN) ..... CPT code 81193 (x1):
    • ETV6-NTRK3 fusion [t(12;15)(p13;q25)]

Testing for one or more specific sarcoma types is available, as well as for all sarcoma types (Full Panel) -- Please specify on the Requisition Form. Fusion partners are confirmed by DNA sequence analysis of the RT-PCR product.

Days Performed

Monday through Friday

Set Up Schedule

All tests not performed daily.

Typical Turnaround

7 days

CPT Codes

81401 (x7), 81193

Lab Area

Institute for Genomic Medicine

Synonyms

  • RT-PCR Fusion Transcript Analysis, fusion, Pediatric Solid Tumors, ARMS, PNET, Sarcoma, Ewing sarcoma, Alveolar rhabdomyosarcoma, Synovial sarcoma, Desmoplastic small round cell tumor, DSRCT, Congenital fibrosarcoma, Cellular mesoblastic nephroma, Primitive neuroectodermal tumor, Soft tissue sarcoma fusion transcript, Sarcoma RT-PCR, RT-PCR, EWSR1, FLI1, FLI-1, ERG, EWS, PAX3, FOXO1, PAX7, SYT, SSX1, SS18, SSX2, WT1, ETV6, NTRK3, Sarcoma gene fusion panel, Sarcoma translocation, IGM Test

Estimated Patient Price

$1,000 - $2,500

Synonyms

  • RT-PCR Fusion Transcript Analysis, fusion, Pediatric Solid Tumors, ARMS, PNET, Sarcoma, Ewing sarcoma, Alveolar rhabdomyosarcoma, Synovial sarcoma, Desmoplastic small round cell tumor, DSRCT, Congenital fibrosarcoma, Cellular mesoblastic nephroma, Primitive neuroectodermal tumor, Soft tissue sarcoma fusion transcript, Sarcoma RT-PCR, RT-PCR, EWSR1, FLI1, FLI-1, ERG, EWS, PAX3, FOXO1, PAX7, SYT, SSX1, SS18, SSX2, WT1, ETV6, NTRK3, Sarcoma gene fusion panel, Sarcoma translocation, IGM Test

CPT Codes

81401 (x7), 81193

Clinical Information

This test detects specific gene fusions associated with the following sarcomas listed below.

  • Ewing sarcoma(EWS) / Primitive neuroectodermal tumors (PNET) ..... CPT codes 81401 (x2):
    • EWSR1-FLI1 (also known as EWS-FLI-1) fusion [t(11;22)(q24;q12)] and
    • EWSR1-ERG (also known as EWS-ERG) fusion [t(21;22)(q22;q12)]
  • Alveolar rhabdomyosarcoma (ARMS) ..... CPT codes 81401 (x2):
    • PAX3-FOXO1 fusion [t(2;13)(q35;q14)] and
    • PAX7-FOXO1 fusion [t(1;13)(p36;q14)]
  • Synovial sarcoma (SS) ..... CPT codes 81401 (x2):
    • SS18-SSX1 (also known as SYT-SSX1) fusion [t(X;18)(p11.2;q11.2)] and
    • SS18-SSX2 (also known as SYT-SSX2) fusion [t(X;18)(p11.2;q11.2)]
  • Desmoplastic small round cell tumor (DSRC) ..... CPT code 81401 (x1):
    • EWSR1-WT1 (also known as EWS-WT1) fusion [t(11;22)(p13;q12)]
  • Congenital fibrosarcoma/Cellular mesoblastic nephroma (CMN) ..... CPT code 81193 (x1):
    • ETV6-NTRK3 fusion [t(12;15)(p13;q25)]

Testing for one or more specific sarcoma types is available, as well as for all sarcoma types (Full Panel) -- Please specify on the Requisition Form. Fusion partners are confirmed by DNA sequence analysis of the RT-PCR product.

Methodology

Reverse transcriptase polymerase chain reaction (RT-PCR), DNA sequencing

Special Collection

FFPE sample is NOT accepted for this test.

Outpatient Submit with Specimen

Oncology Genetic Requisition

Collect

Specimen Type Type of Container Volume of Specimen Status
Tissue (Snap-frozen) Tissue cassette   Preferred
Tissue (Snap-frozen) Cryogenic tube   Alternate
OCT-embedded tissue Tissue cassette   Preferred
OCT-embedded tissue Cryogenic tube   Alternate

Minimum Volume

Specimen Type Type of Container Minimum Volume
Tissue (Snap-frozen) Tissue cassette  
Tissue (Snap-frozen) Cryogenic tube  
OCT-embedded tissue Tissue cassette  
OCT-embedded tissue Cryogenic tube  

Inpatient Specimen Preparation

Tissue (Snap-frozen): Keep frozen
                                    Do not add fixative
                                    Immediately place on dry ice and transport frozen
                                    Transport to laboratory as soon as possible
                                    Protect from heat

OCT-embedded tissue: Keep frozen
                                      Transport to laboratory as soon as possible
                                      Protect from heat
                                      Immediately place on dry ice and transport frozen

Outpatient Specimen Preparation

Tissue (Snap-frozen): Keep frozen
                                    Do not add fixative
                                    Immediately place on dry ice and transport frozen
                                    Transport to laboratory as soon as possible
                                    Protect from heat

OCT-embedded tissue: Keep frozen
                                      Transport to laboratory as soon as possible
                                      Protect from heat
                                      Immediately place on dry ice and transport frozen
 

InLab Processing

TIME SENSITIVE SPECIMEN. Send to Molecular Genetics Lab ASAP with all submitted paperwork. For Non-EPIC lab orders, lab staff to order GENSP in Sunquest.

Stability

Tissue (Snap-frozen): Frozen 6 month(s)

OCT-embedded tissue: Frozen 6 month(s)
 

Unacceptable Conditions

Delayed or improper handling, Tissue degradation, Formalin Fixed Tissue, Thawed frozen specimen, Wrong type of specimen

Days Performed

Monday through Friday

Set Up Schedule

All tests not performed daily.

Typical Turnaround

7 days

Remarks

If you are an external healthcare provider with no access to Nationwide Children’s Epic system, submission of a completed Oncology Genetic Test Requisition Form is required;. If you are an internal ordering provider with access to Nationwide Children’s Epic system, no requisition form is required; please place the lab order electronically in Epic.

Only snap-frozen or OCT-embedded tissue is accepted for this testing. Paraffin-embedded tissue is NOT accepted for this testing. Handle samples using RNA precautions and transport on dry ice. Please send any available pathology reports, even if preliminary. Ship all samples frozen via overnight delivery. Saturday deliveries are accepted. Please check "Saturday Delivery" on shipment label. Please call (614) 722-5321 with questions.

Clinical Information

This test detects specific gene fusions associated with the following sarcomas listed below.

  • Ewing sarcoma(EWS) / Primitive neuroectodermal tumors (PNET) ..... CPT codes 81401 (x2):
    • EWSR1-FLI1 (also known as EWS-FLI-1) fusion [t(11;22)(q24;q12)] and
    • EWSR1-ERG (also known as EWS-ERG) fusion [t(21;22)(q22;q12)]
  • Alveolar rhabdomyosarcoma (ARMS) ..... CPT codes 81401 (x2):
    • PAX3-FOXO1 fusion [t(2;13)(q35;q14)] and
    • PAX7-FOXO1 fusion [t(1;13)(p36;q14)]
  • Synovial sarcoma (SS) ..... CPT codes 81401 (x2):
    • SS18-SSX1 (also known as SYT-SSX1) fusion [t(X;18)(p11.2;q11.2)] and
    • SS18-SSX2 (also known as SYT-SSX2) fusion [t(X;18)(p11.2;q11.2)]
  • Desmoplastic small round cell tumor (DSRC) ..... CPT code 81401 (x1):
    • EWSR1-WT1 (also known as EWS-WT1) fusion [t(11;22)(p13;q12)]
  • Congenital fibrosarcoma/Cellular mesoblastic nephroma (CMN) ..... CPT code 81193 (x1):
    • ETV6-NTRK3 fusion [t(12;15)(p13;q25)]

Testing for one or more specific sarcoma types is available, as well as for all sarcoma types (Full Panel) -- Please specify on the Requisition Form. Fusion partners are confirmed by DNA sequence analysis of the RT-PCR product.

Synonyms

  • RT-PCR Fusion Transcript Analysis, fusion, Pediatric Solid Tumors, ARMS, PNET, Sarcoma, Ewing sarcoma, Alveolar rhabdomyosarcoma, Synovial sarcoma, Desmoplastic small round cell tumor, DSRCT, Congenital fibrosarcoma, Cellular mesoblastic nephroma, Primitive neuroectodermal tumor, Soft tissue sarcoma fusion transcript, Sarcoma RT-PCR, RT-PCR, EWSR1, FLI1, FLI-1, ERG, EWS, PAX3, FOXO1, PAX7, SYT, SSX1, SS18, SSX2, WT1, ETV6, NTRK3, Sarcoma gene fusion panel, Sarcoma translocation, IGM Test

Methodology

Reverse transcriptase polymerase chain reaction (RT-PCR), DNA sequencing

CPT Codes

81401 (x7), 81193

Estimated Patient Price

$1,000 - $2,500

DC Code

5321

Downtime Availability

4-Not available
Outpatient Requirements

Special Collection

FFPE sample is NOT accepted for this test.

Outpatient Submit with Specimen

Oncology Genetic Requisition

Collect

Specimen Type Type of Container Volume of Specimen Status
Tissue (Snap-frozen) Tissue cassette   Preferred
Tissue (Snap-frozen) Cryogenic tube   Alternate
OCT-embedded tissue Tissue cassette   Preferred
OCT-embedded tissue Cryogenic tube   Alternate

Outpatient Specimen Preparation

Tissue (Snap-frozen): Keep frozen
                                    Do not add fixative
                                    Immediately place on dry ice and transport frozen
                                    Transport to laboratory as soon as possible
                                    Protect from heat

OCT-embedded tissue: Keep frozen
                                      Transport to laboratory as soon as possible
                                      Protect from heat
                                      Immediately place on dry ice and transport frozen
 

Unacceptable Conditions

Delayed or improper handling, Tissue degradation, Formalin Fixed Tissue, Thawed frozen specimen, Wrong type of specimen

Stability

Tissue (Snap-frozen): Frozen 6 month(s)

OCT-embedded tissue: Frozen 6 month(s)
 

Remarks

If you are an external healthcare provider with no access to Nationwide Children’s Epic system, submission of a completed Oncology Genetic Test Requisition Form is required;. If you are an internal ordering provider with access to Nationwide Children’s Epic system, no requisition form is required; please place the lab order electronically in Epic.

Only snap-frozen or OCT-embedded tissue is accepted for this testing. Paraffin-embedded tissue is NOT accepted for this testing. Handle samples using RNA precautions and transport on dry ice. Please send any available pathology reports, even if preliminary. Ship all samples frozen via overnight delivery. Saturday deliveries are accepted. Please check "Saturday Delivery" on shipment label. Please call (614) 722-5321 with questions.

Clinical Information

This test detects specific gene fusions associated with the following sarcomas listed below.

  • Ewing sarcoma(EWS) / Primitive neuroectodermal tumors (PNET) ..... CPT codes 81401 (x2):
    • EWSR1-FLI1 (also known as EWS-FLI-1) fusion [t(11;22)(q24;q12)] and
    • EWSR1-ERG (also known as EWS-ERG) fusion [t(21;22)(q22;q12)]
  • Alveolar rhabdomyosarcoma (ARMS) ..... CPT codes 81401 (x2):
    • PAX3-FOXO1 fusion [t(2;13)(q35;q14)] and
    • PAX7-FOXO1 fusion [t(1;13)(p36;q14)]
  • Synovial sarcoma (SS) ..... CPT codes 81401 (x2):
    • SS18-SSX1 (also known as SYT-SSX1) fusion [t(X;18)(p11.2;q11.2)] and
    • SS18-SSX2 (also known as SYT-SSX2) fusion [t(X;18)(p11.2;q11.2)]
  • Desmoplastic small round cell tumor (DSRC) ..... CPT code 81401 (x1):
    • EWSR1-WT1 (also known as EWS-WT1) fusion [t(11;22)(p13;q12)]
  • Congenital fibrosarcoma/Cellular mesoblastic nephroma (CMN) ..... CPT code 81193 (x1):
    • ETV6-NTRK3 fusion [t(12;15)(p13;q25)]

Testing for one or more specific sarcoma types is available, as well as for all sarcoma types (Full Panel) -- Please specify on the Requisition Form. Fusion partners are confirmed by DNA sequence analysis of the RT-PCR product.

Days Performed

Monday through Friday

Set Up Schedule

All tests not performed daily.

Typical Turnaround

7 days

Lab Area

Institute for Genomic Medicine

Methodology

Reverse transcriptase polymerase chain reaction (RT-PCR), DNA sequencing

CPT Codes

81401 (x7), 81193

Synonyms

  • RT-PCR Fusion Transcript Analysis, fusion, Pediatric Solid Tumors, ARMS, PNET, Sarcoma, Ewing sarcoma, Alveolar rhabdomyosarcoma, Synovial sarcoma, Desmoplastic small round cell tumor, DSRCT, Congenital fibrosarcoma, Cellular mesoblastic nephroma, Primitive neuroectodermal tumor, Soft tissue sarcoma fusion transcript, Sarcoma RT-PCR, RT-PCR, EWSR1, FLI1, FLI-1, ERG, EWS, PAX3, FOXO1, PAX7, SYT, SSX1, SS18, SSX2, WT1, ETV6, NTRK3, Sarcoma gene fusion panel, Sarcoma translocation, IGM Test
Inpatient Requirements

Special Collection

FFPE sample is NOT accepted for this test.

Collect

Specimen Type Type of Container Volume of Specimen Status
Tissue (Snap-frozen) Tissue cassette   Preferred
Tissue (Snap-frozen) Cryogenic tube   Alternate
OCT-embedded tissue Tissue cassette   Preferred
OCT-embedded tissue Cryogenic tube   Alternate

Minimum Volume

Specimen Type Type of Container Minimum Volume
Tissue (Snap-frozen) Tissue cassette  
Tissue (Snap-frozen) Cryogenic tube  
OCT-embedded tissue Tissue cassette  
OCT-embedded tissue Cryogenic tube  

Inpatient Specimen Preparation

Tissue (Snap-frozen): Keep frozen
                                    Do not add fixative
                                    Immediately place on dry ice and transport frozen
                                    Transport to laboratory as soon as possible
                                    Protect from heat

OCT-embedded tissue: Keep frozen
                                      Transport to laboratory as soon as possible
                                      Protect from heat
                                      Immediately place on dry ice and transport frozen

Unacceptable Conditions

Delayed or improper handling, Tissue degradation, Formalin Fixed Tissue, Thawed frozen specimen, Wrong type of specimen

Stability

Tissue (Snap-frozen): Frozen 6 month(s)

OCT-embedded tissue: Frozen 6 month(s)
 

Remarks

If you are an external healthcare provider with no access to Nationwide Children’s Epic system, submission of a completed Oncology Genetic Test Requisition Form is required;. If you are an internal ordering provider with access to Nationwide Children’s Epic system, no requisition form is required; please place the lab order electronically in Epic.

Only snap-frozen or OCT-embedded tissue is accepted for this testing. Paraffin-embedded tissue is NOT accepted for this testing. Handle samples using RNA precautions and transport on dry ice. Please send any available pathology reports, even if preliminary. Ship all samples frozen via overnight delivery. Saturday deliveries are accepted. Please check "Saturday Delivery" on shipment label. Please call (614) 722-5321 with questions.

Clinical Information

This test detects specific gene fusions associated with the following sarcomas listed below.

  • Ewing sarcoma(EWS) / Primitive neuroectodermal tumors (PNET) ..... CPT codes 81401 (x2):
    • EWSR1-FLI1 (also known as EWS-FLI-1) fusion [t(11;22)(q24;q12)] and
    • EWSR1-ERG (also known as EWS-ERG) fusion [t(21;22)(q22;q12)]
  • Alveolar rhabdomyosarcoma (ARMS) ..... CPT codes 81401 (x2):
    • PAX3-FOXO1 fusion [t(2;13)(q35;q14)] and
    • PAX7-FOXO1 fusion [t(1;13)(p36;q14)]
  • Synovial sarcoma (SS) ..... CPT codes 81401 (x2):
    • SS18-SSX1 (also known as SYT-SSX1) fusion [t(X;18)(p11.2;q11.2)] and
    • SS18-SSX2 (also known as SYT-SSX2) fusion [t(X;18)(p11.2;q11.2)]
  • Desmoplastic small round cell tumor (DSRC) ..... CPT code 81401 (x1):
    • EWSR1-WT1 (also known as EWS-WT1) fusion [t(11;22)(p13;q12)]
  • Congenital fibrosarcoma/Cellular mesoblastic nephroma (CMN) ..... CPT code 81193 (x1):
    • ETV6-NTRK3 fusion [t(12;15)(p13;q25)]

Testing for one or more specific sarcoma types is available, as well as for all sarcoma types (Full Panel) -- Please specify on the Requisition Form. Fusion partners are confirmed by DNA sequence analysis of the RT-PCR product.

Days Performed

Monday through Friday

Set Up Schedule

All tests not performed daily.

Typical Turnaround

7 days

CPT Codes

81401 (x7), 81193

Lab Area

Institute for Genomic Medicine

Synonyms

  • RT-PCR Fusion Transcript Analysis, fusion, Pediatric Solid Tumors, ARMS, PNET, Sarcoma, Ewing sarcoma, Alveolar rhabdomyosarcoma, Synovial sarcoma, Desmoplastic small round cell tumor, DSRCT, Congenital fibrosarcoma, Cellular mesoblastic nephroma, Primitive neuroectodermal tumor, Soft tissue sarcoma fusion transcript, Sarcoma RT-PCR, RT-PCR, EWSR1, FLI1, FLI-1, ERG, EWS, PAX3, FOXO1, PAX7, SYT, SSX1, SS18, SSX2, WT1, ETV6, NTRK3, Sarcoma gene fusion panel, Sarcoma translocation, IGM Test

Estimated Patient Price

$1,000 - $2,500
Overview/Billing

Synonyms

  • RT-PCR Fusion Transcript Analysis, fusion, Pediatric Solid Tumors, ARMS, PNET, Sarcoma, Ewing sarcoma, Alveolar rhabdomyosarcoma, Synovial sarcoma, Desmoplastic small round cell tumor, DSRCT, Congenital fibrosarcoma, Cellular mesoblastic nephroma, Primitive neuroectodermal tumor, Soft tissue sarcoma fusion transcript, Sarcoma RT-PCR, RT-PCR, EWSR1, FLI1, FLI-1, ERG, EWS, PAX3, FOXO1, PAX7, SYT, SSX1, SS18, SSX2, WT1, ETV6, NTRK3, Sarcoma gene fusion panel, Sarcoma translocation, IGM Test

CPT Codes

81401 (x7), 81193
Interpretation

Clinical Information

This test detects specific gene fusions associated with the following sarcomas listed below.

  • Ewing sarcoma(EWS) / Primitive neuroectodermal tumors (PNET) ..... CPT codes 81401 (x2):
    • EWSR1-FLI1 (also known as EWS-FLI-1) fusion [t(11;22)(q24;q12)] and
    • EWSR1-ERG (also known as EWS-ERG) fusion [t(21;22)(q22;q12)]
  • Alveolar rhabdomyosarcoma (ARMS) ..... CPT codes 81401 (x2):
    • PAX3-FOXO1 fusion [t(2;13)(q35;q14)] and
    • PAX7-FOXO1 fusion [t(1;13)(p36;q14)]
  • Synovial sarcoma (SS) ..... CPT codes 81401 (x2):
    • SS18-SSX1 (also known as SYT-SSX1) fusion [t(X;18)(p11.2;q11.2)] and
    • SS18-SSX2 (also known as SYT-SSX2) fusion [t(X;18)(p11.2;q11.2)]
  • Desmoplastic small round cell tumor (DSRC) ..... CPT code 81401 (x1):
    • EWSR1-WT1 (also known as EWS-WT1) fusion [t(11;22)(p13;q12)]
  • Congenital fibrosarcoma/Cellular mesoblastic nephroma (CMN) ..... CPT code 81193 (x1):
    • ETV6-NTRK3 fusion [t(12;15)(p13;q25)]

Testing for one or more specific sarcoma types is available, as well as for all sarcoma types (Full Panel) -- Please specify on the Requisition Form. Fusion partners are confirmed by DNA sequence analysis of the RT-PCR product.

Methodology

Reverse transcriptase polymerase chain reaction (RT-PCR), DNA sequencing
NCH Lab Only

Special Collection

FFPE sample is NOT accepted for this test.

Outpatient Submit with Specimen

Oncology Genetic Requisition

Collect

Specimen Type Type of Container Volume of Specimen Status
Tissue (Snap-frozen) Tissue cassette   Preferred
Tissue (Snap-frozen) Cryogenic tube   Alternate
OCT-embedded tissue Tissue cassette   Preferred
OCT-embedded tissue Cryogenic tube   Alternate

Minimum Volume

Specimen Type Type of Container Minimum Volume
Tissue (Snap-frozen) Tissue cassette  
Tissue (Snap-frozen) Cryogenic tube  
OCT-embedded tissue Tissue cassette  
OCT-embedded tissue Cryogenic tube  

Inpatient Specimen Preparation

Tissue (Snap-frozen): Keep frozen
                                    Do not add fixative
                                    Immediately place on dry ice and transport frozen
                                    Transport to laboratory as soon as possible
                                    Protect from heat

OCT-embedded tissue: Keep frozen
                                      Transport to laboratory as soon as possible
                                      Protect from heat
                                      Immediately place on dry ice and transport frozen

Outpatient Specimen Preparation

Tissue (Snap-frozen): Keep frozen
                                    Do not add fixative
                                    Immediately place on dry ice and transport frozen
                                    Transport to laboratory as soon as possible
                                    Protect from heat

OCT-embedded tissue: Keep frozen
                                      Transport to laboratory as soon as possible
                                      Protect from heat
                                      Immediately place on dry ice and transport frozen
 

InLab Processing

TIME SENSITIVE SPECIMEN. Send to Molecular Genetics Lab ASAP with all submitted paperwork. For Non-EPIC lab orders, lab staff to order GENSP in Sunquest.

Stability

Tissue (Snap-frozen): Frozen 6 month(s)

OCT-embedded tissue: Frozen 6 month(s)
 

Unacceptable Conditions

Delayed or improper handling, Tissue degradation, Formalin Fixed Tissue, Thawed frozen specimen, Wrong type of specimen

Days Performed

Monday through Friday

Set Up Schedule

All tests not performed daily.

Typical Turnaround

7 days

Remarks

If you are an external healthcare provider with no access to Nationwide Children’s Epic system, submission of a completed Oncology Genetic Test Requisition Form is required;. If you are an internal ordering provider with access to Nationwide Children’s Epic system, no requisition form is required; please place the lab order electronically in Epic.

Only snap-frozen or OCT-embedded tissue is accepted for this testing. Paraffin-embedded tissue is NOT accepted for this testing. Handle samples using RNA precautions and transport on dry ice. Please send any available pathology reports, even if preliminary. Ship all samples frozen via overnight delivery. Saturday deliveries are accepted. Please check "Saturday Delivery" on shipment label. Please call (614) 722-5321 with questions.

Clinical Information

This test detects specific gene fusions associated with the following sarcomas listed below.

  • Ewing sarcoma(EWS) / Primitive neuroectodermal tumors (PNET) ..... CPT codes 81401 (x2):
    • EWSR1-FLI1 (also known as EWS-FLI-1) fusion [t(11;22)(q24;q12)] and
    • EWSR1-ERG (also known as EWS-ERG) fusion [t(21;22)(q22;q12)]
  • Alveolar rhabdomyosarcoma (ARMS) ..... CPT codes 81401 (x2):
    • PAX3-FOXO1 fusion [t(2;13)(q35;q14)] and
    • PAX7-FOXO1 fusion [t(1;13)(p36;q14)]
  • Synovial sarcoma (SS) ..... CPT codes 81401 (x2):
    • SS18-SSX1 (also known as SYT-SSX1) fusion [t(X;18)(p11.2;q11.2)] and
    • SS18-SSX2 (also known as SYT-SSX2) fusion [t(X;18)(p11.2;q11.2)]
  • Desmoplastic small round cell tumor (DSRC) ..... CPT code 81401 (x1):
    • EWSR1-WT1 (also known as EWS-WT1) fusion [t(11;22)(p13;q12)]
  • Congenital fibrosarcoma/Cellular mesoblastic nephroma (CMN) ..... CPT code 81193 (x1):
    • ETV6-NTRK3 fusion [t(12;15)(p13;q25)]

Testing for one or more specific sarcoma types is available, as well as for all sarcoma types (Full Panel) -- Please specify on the Requisition Form. Fusion partners are confirmed by DNA sequence analysis of the RT-PCR product.

Synonyms

  • RT-PCR Fusion Transcript Analysis, fusion, Pediatric Solid Tumors, ARMS, PNET, Sarcoma, Ewing sarcoma, Alveolar rhabdomyosarcoma, Synovial sarcoma, Desmoplastic small round cell tumor, DSRCT, Congenital fibrosarcoma, Cellular mesoblastic nephroma, Primitive neuroectodermal tumor, Soft tissue sarcoma fusion transcript, Sarcoma RT-PCR, RT-PCR, EWSR1, FLI1, FLI-1, ERG, EWS, PAX3, FOXO1, PAX7, SYT, SSX1, SS18, SSX2, WT1, ETV6, NTRK3, Sarcoma gene fusion panel, Sarcoma translocation, IGM Test

Methodology

Reverse transcriptase polymerase chain reaction (RT-PCR), DNA sequencing

CPT Codes

81401 (x7), 81193

Estimated Patient Price

$1,000 - $2,500

DC Code

5321

Downtime Availability

4-Not available

Lab Area

Lab Area
Institute for Genomic Medicine

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