| Specimen Type | Type of Container | Volume of Specimen | Status |
|---|---|---|---|
| Unstained tissue slides | Unstained slides and consecutive cut H&E | 10 slides, 10 microns thick | Preferred |
| Paraffin embedded tissue | Paraffin block | 1 block | Preferred |
| Tissue (Snap-frozen) | Tissue cassette | 50 mg | Alternate |
| Tissue (Snap-frozen) | Cryogenic tube | 50 mg | Alternate |
| OCT-embedded tissue | Tissue cassette | 50 mg | Alternate |
| OCT-embedded tissue | Cryogenic tube | 50 mg | Alternate |
| Tissue (Fresh) | Tissue culture transport media | 50 mg | Alternate |
| Tissue (Fresh) | Sterile container with saline | 50 mg | Alternate |
| Bone Marrow | 4 mL Purple tube (EDTA) | 4 mL | Alternate |
| Tissue scrolls (FFPE) Sterile container | Sterile container | 10-15 scrolls | Alternate |
| RNA *Must be extracted in a CLIA approved lab | Microcentrifuge tube | Contact IGM lab | Alternate |
Unstained slides:
Keep at room temperature
Protect from heat
Must be accompanied by H&E slide from adjacent section
Samples decalcified with a strong acid are not accepted. EDTA-based decalcification methods (such as Formical) are acceptable.
Paraffin embedded tissue:
Protect from heat
Keep at room temperature
Tissue (Snap-frozen):
Freeze immediately after collection.
Transport to laboratory as soon as possible
Protect from heat
Keep frozen
OCT-embedded tissue:
Keep frozen
Transport to laboratory as soon as possible
Protect from heat
Freeze immediately after collection.
Tissue (Fresh):
Refrigerate after collection. If delay in transport is greater than 24 hours, freeze.
Transport to lab on ice immediately after collection
Protect from heat
Do not add fixative
Do not formalin fix the sample
Keep refrigerated
Bone marrow:
If delay in transport is greater than 24 hours, refrigerate
Transport to the lab immediately after collection
Room temperature 24 hours
If you are an external healthcare provider with no access to Nationwide Children’s Epic system, submission of a completed Oncology Genetic Test Requisition Form is required. If you are an internal ordering provider with access to Nationwide Children’s Epic system, no requisition form is required; please place the lab order electronically in Epic.
Submission of 10-15 unstained slides at 5-10 microns is recommended. If submitting tissue scroll specimen, please use this Tissue Scroll Calculator tool to determine the thickness and number of tissue scrolls to send to the laboratory.
SPECIMEN INFORMATION:
Fresh and snap-frozen tissue specimens must contain minimum of 10% disease content. Formalin-fixed paraffin embedded (FFPE) tissue block and FFPE tissue scroll specimens must contain minimum of 25% disease content. Please submit the pathology report from the submitted sample. Internal pathology review will also be performed on non-frozen samples to determine the tumor content in specimen.
If sending FFPE tissue scrolls, an H&E slide from a consecutive cut is also required. Please see scroll calculator application above.
All specimens must be labeled with a patient name and one other identifier (DOB, MRN, Specimen ID#, etc). Sample acquisition prior to receiving therapy is strongly preferred.
SPECIMEN SHIPPING:
Specimens must be shipped as soon as possible after collection due to time-sensitive specimen stability. Ship all samples via overnight courier. Whenever possible, send specimen immediately after collection. For fresh tumor tissue, ship the sample refrigerated using cold packs. If specimen cannot be sent on the collection date, then snap-freeze the tumor tissue immediately after collection and ship the sample frozen (bury the entire tissue in dry ice). Lab must receive specimen within 48 hours. FFPE tissue and FFPE tissue scrolls should be sent at room temperature via overnight shipping. Saturday deliveries are accepted; please check Saturday Delivery on shipment labels.
| Specimen Type | Type of Container | Volume of Specimen | Status |
|---|---|---|---|
| Unstained tissue slides | Unstained slides and consecutive cut H&E | 10 slides, 10 microns thick | Preferred |
| Paraffin embedded tissue | Paraffin block | 1 block | Preferred |
| Tissue (Snap-frozen) | Tissue cassette | 50 mg | Alternate |
| Tissue (Snap-frozen) | Cryogenic tube | 50 mg | Alternate |
| OCT-embedded tissue | Tissue cassette | 50 mg | Alternate |
| OCT-embedded tissue | Cryogenic tube | 50 mg | Alternate |
| Tissue (Fresh) | Tissue culture transport media | 50 mg | Alternate |
| Tissue (Fresh) | Sterile container with saline | 50 mg | Alternate |
| Bone Marrow | 4 mL Purple tube (EDTA) | 4 mL | Alternate |
| Tissue scrolls (FFPE) Sterile container | Sterile container | 10-15 scrolls | Alternate |
| RNA *Must be extracted in a CLIA approved lab | Microcentrifuge tube | Contact IGM lab | Alternate |
| Specimen Type | Type of Container | Minimum Volume |
|---|---|---|
| Unstained tissue slides | Unstained slides and consecutive cut H&E | 10 slides, 10 microns thick |
| Paraffin embedded tissue | Paraffin block | 1 block |
| Tissue (Snap-frozen) | Tissue cassette | 50 mg |
| Tissue (Snap-frozen) | Cryogenic tube | 50 mg |
| OCT-embedded tissue | Tissue cassette | 50 mg |
| OCT-embedded tissue | Cryogenic tube | 50 mg |
| Tissue (Fresh) | Tissue culture transport media | 50 mg |
| Tissue (Fresh) | Sterile container with saline | 50 mg |
| Bone Marrow | 4 mL Purple tube (EDTA) | 4 mL |
| Tissue scrolls (FFPE) Sterile container | Sterile container | 10-15 scrolls |
| RNA *Must be extracted in a CLIA approved lab | Microcentrifuge tube | Contact IGM lab |
If you are an external healthcare provider with no access to Nationwide Children’s Epic system, submission of a completed Oncology Genetic Test Requisition Form is required. If you are an internal ordering provider with access to Nationwide Children’s Epic system, no requisition form is required; please place the lab order electronically in Epic.
Submission of 10-15 unstained slides at 5-10 microns is recommended. If submitting tissue scroll specimen, please use this Tissue Scroll Calculator tool to determine the thickness and number of tissue scrolls to send to the laboratory.
SPECIMEN INFORMATION:
Fresh and snap-frozen tissue specimens must contain minimum of 10% disease content. Formalin-fixed paraffin embedded (FFPE) tissue block and FFPE tissue scroll specimens must contain minimum of 25% disease content. Please submit the pathology report from the submitted sample. Internal pathology review will also be performed on non-frozen samples to determine the tumor content in specimen.
If sending FFPE tissue scrolls, an H&E slide from a consecutive cut is also required. Please see scroll calculator application above.
All specimens must be labeled with a patient name and one other identifier (DOB, MRN, Specimen ID#, etc). Sample acquisition prior to receiving therapy is strongly preferred.
SPECIMEN SHIPPING:
Specimens must be shipped as soon as possible after collection due to time-sensitive specimen stability. Ship all samples via overnight courier. Whenever possible, send specimen immediately after collection. For fresh tumor tissue, ship the sample refrigerated using cold packs. If specimen cannot be sent on the collection date, then snap-freeze the tumor tissue immediately after collection and ship the sample frozen (bury the entire tissue in dry ice). Lab must receive specimen within 48 hours. FFPE tissue and FFPE tissue scrolls should be sent at room temperature via overnight shipping. Saturday deliveries are accepted; please check Saturday Delivery on shipment labels.
| Specimen Type | Type of Container | Volume of Specimen | Status |
|---|---|---|---|
| Unstained tissue slides | Unstained slides and consecutive cut H&E | 10 slides, 10 microns thick | Preferred |
| Paraffin embedded tissue | Paraffin block | 1 block | Preferred |
| Tissue (Snap-frozen) | Tissue cassette | 50 mg | Alternate |
| Tissue (Snap-frozen) | Cryogenic tube | 50 mg | Alternate |
| OCT-embedded tissue | Tissue cassette | 50 mg | Alternate |
| OCT-embedded tissue | Cryogenic tube | 50 mg | Alternate |
| Tissue (Fresh) | Tissue culture transport media | 50 mg | Alternate |
| Tissue (Fresh) | Sterile container with saline | 50 mg | Alternate |
| Bone Marrow | 4 mL Purple tube (EDTA) | 4 mL | Alternate |
| Tissue scrolls (FFPE) Sterile container | Sterile container | 10-15 scrolls | Alternate |
| RNA *Must be extracted in a CLIA approved lab | Microcentrifuge tube | Contact IGM lab | Alternate |
| Specimen Type | Type of Container | Minimum Volume |
|---|---|---|
| Unstained tissue slides | Unstained slides and consecutive cut H&E | 10 slides, 10 microns thick |
| Paraffin embedded tissue | Paraffin block | 1 block |
| Tissue (Snap-frozen) | Tissue cassette | 50 mg |
| Tissue (Snap-frozen) | Cryogenic tube | 50 mg |
| OCT-embedded tissue | Tissue cassette | 50 mg |
| OCT-embedded tissue | Cryogenic tube | 50 mg |
| Tissue (Fresh) | Tissue culture transport media | 50 mg |
| Tissue (Fresh) | Sterile container with saline | 50 mg |
| Bone Marrow | 4 mL Purple tube (EDTA) | 4 mL |
| Tissue scrolls (FFPE) Sterile container | Sterile container | 10-15 scrolls |
| RNA *Must be extracted in a CLIA approved lab | Microcentrifuge tube | Contact IGM lab |
Unstained slides:
Keep at room temperature
Protect from heat
Must be accompanied by H&E slide from adjacent section
Samples decalcified with a strong acid are not accepted. EDTA-based decalcification methods (such as Formical) are acceptable.
Paraffin embedded tissue:
Protect from heat
Keep at room temperature
Tissue (Snap-frozen):
Freeze immediately after collection.
Transport to laboratory as soon as possible
Protect from heat
Keep frozen
OCT-embedded tissue:
Keep frozen
Transport to laboratory as soon as possible
Protect from heat
Freeze immediately after collection.
Tissue (Fresh):
Refrigerate after collection. If delay in transport is greater than 24 hours, freeze.
Transport to lab on ice immediately after collection
Protect from heat
Do not add fixative
Do not formalin fix the sample
Keep refrigerated
Bone marrow:
If delay in transport is greater than 24 hours, refrigerate
Transport to the lab immediately after collection
Room temperature 24 hours
If you are an external healthcare provider with no access to Nationwide Children’s Epic system, submission of a completed Oncology Genetic Test Requisition Form is required. If you are an internal ordering provider with access to Nationwide Children’s Epic system, no requisition form is required; please place the lab order electronically in Epic.
Submission of 10-15 unstained slides at 5-10 microns is recommended. If submitting tissue scroll specimen, please use this Tissue Scroll Calculator tool to determine the thickness and number of tissue scrolls to send to the laboratory.
SPECIMEN INFORMATION:
Fresh and snap-frozen tissue specimens must contain minimum of 10% disease content. Formalin-fixed paraffin embedded (FFPE) tissue block and FFPE tissue scroll specimens must contain minimum of 25% disease content. Please submit the pathology report from the submitted sample. Internal pathology review will also be performed on non-frozen samples to determine the tumor content in specimen.
If sending FFPE tissue scrolls, an H&E slide from a consecutive cut is also required. Please see scroll calculator application above.
All specimens must be labeled with a patient name and one other identifier (DOB, MRN, Specimen ID#, etc). Sample acquisition prior to receiving therapy is strongly preferred.
SPECIMEN SHIPPING:
Specimens must be shipped as soon as possible after collection due to time-sensitive specimen stability. Ship all samples via overnight courier. Whenever possible, send specimen immediately after collection. For fresh tumor tissue, ship the sample refrigerated using cold packs. If specimen cannot be sent on the collection date, then snap-freeze the tumor tissue immediately after collection and ship the sample frozen (bury the entire tissue in dry ice). Lab must receive specimen within 48 hours. FFPE tissue and FFPE tissue scrolls should be sent at room temperature via overnight shipping. Saturday deliveries are accepted; please check Saturday Delivery on shipment labels.
| Outpatient Requirements |
| Specimen Type | Type of Container | Volume of Specimen | Status |
|---|---|---|---|
| Unstained tissue slides | Unstained slides and consecutive cut H&E | 10 slides, 10 microns thick | Preferred |
| Paraffin embedded tissue | Paraffin block | 1 block | Preferred |
| Tissue (Snap-frozen) | Tissue cassette | 50 mg | Alternate |
| Tissue (Snap-frozen) | Cryogenic tube | 50 mg | Alternate |
| OCT-embedded tissue | Tissue cassette | 50 mg | Alternate |
| OCT-embedded tissue | Cryogenic tube | 50 mg | Alternate |
| Tissue (Fresh) | Tissue culture transport media | 50 mg | Alternate |
| Tissue (Fresh) | Sterile container with saline | 50 mg | Alternate |
| Bone Marrow | 4 mL Purple tube (EDTA) | 4 mL | Alternate |
| Tissue scrolls (FFPE) Sterile container | Sterile container | 10-15 scrolls | Alternate |
| RNA *Must be extracted in a CLIA approved lab | Microcentrifuge tube | Contact IGM lab | Alternate |
Unstained slides:
Keep at room temperature
Protect from heat
Must be accompanied by H&E slide from adjacent section
Samples decalcified with a strong acid are not accepted. EDTA-based decalcification methods (such as Formical) are acceptable.
Paraffin embedded tissue:
Protect from heat
Keep at room temperature
Tissue (Snap-frozen):
Freeze immediately after collection.
Transport to laboratory as soon as possible
Protect from heat
Keep frozen
OCT-embedded tissue:
Keep frozen
Transport to laboratory as soon as possible
Protect from heat
Freeze immediately after collection.
Tissue (Fresh):
Refrigerate after collection. If delay in transport is greater than 24 hours, freeze.
Transport to lab on ice immediately after collection
Protect from heat
Do not add fixative
Do not formalin fix the sample
Keep refrigerated
Bone marrow:
If delay in transport is greater than 24 hours, refrigerate
Transport to the lab immediately after collection
Room temperature 24 hours
If you are an external healthcare provider with no access to Nationwide Children’s Epic system, submission of a completed Oncology Genetic Test Requisition Form is required. If you are an internal ordering provider with access to Nationwide Children’s Epic system, no requisition form is required; please place the lab order electronically in Epic.
Submission of 10-15 unstained slides at 5-10 microns is recommended. If submitting tissue scroll specimen, please use this Tissue Scroll Calculator tool to determine the thickness and number of tissue scrolls to send to the laboratory.
SPECIMEN INFORMATION:
Fresh and snap-frozen tissue specimens must contain minimum of 10% disease content. Formalin-fixed paraffin embedded (FFPE) tissue block and FFPE tissue scroll specimens must contain minimum of 25% disease content. Please submit the pathology report from the submitted sample. Internal pathology review will also be performed on non-frozen samples to determine the tumor content in specimen.
If sending FFPE tissue scrolls, an H&E slide from a consecutive cut is also required. Please see scroll calculator application above.
All specimens must be labeled with a patient name and one other identifier (DOB, MRN, Specimen ID#, etc). Sample acquisition prior to receiving therapy is strongly preferred.
SPECIMEN SHIPPING:
Specimens must be shipped as soon as possible after collection due to time-sensitive specimen stability. Ship all samples via overnight courier. Whenever possible, send specimen immediately after collection. For fresh tumor tissue, ship the sample refrigerated using cold packs. If specimen cannot be sent on the collection date, then snap-freeze the tumor tissue immediately after collection and ship the sample frozen (bury the entire tissue in dry ice). Lab must receive specimen within 48 hours. FFPE tissue and FFPE tissue scrolls should be sent at room temperature via overnight shipping. Saturday deliveries are accepted; please check Saturday Delivery on shipment labels.
| Inpatient Requirements |
| Specimen Type | Type of Container | Volume of Specimen | Status |
|---|---|---|---|
| Unstained tissue slides | Unstained slides and consecutive cut H&E | 10 slides, 10 microns thick | Preferred |
| Paraffin embedded tissue | Paraffin block | 1 block | Preferred |
| Tissue (Snap-frozen) | Tissue cassette | 50 mg | Alternate |
| Tissue (Snap-frozen) | Cryogenic tube | 50 mg | Alternate |
| OCT-embedded tissue | Tissue cassette | 50 mg | Alternate |
| OCT-embedded tissue | Cryogenic tube | 50 mg | Alternate |
| Tissue (Fresh) | Tissue culture transport media | 50 mg | Alternate |
| Tissue (Fresh) | Sterile container with saline | 50 mg | Alternate |
| Bone Marrow | 4 mL Purple tube (EDTA) | 4 mL | Alternate |
| Tissue scrolls (FFPE) Sterile container | Sterile container | 10-15 scrolls | Alternate |
| RNA *Must be extracted in a CLIA approved lab | Microcentrifuge tube | Contact IGM lab | Alternate |
| Specimen Type | Type of Container | Minimum Volume |
|---|---|---|
| Unstained tissue slides | Unstained slides and consecutive cut H&E | 10 slides, 10 microns thick |
| Paraffin embedded tissue | Paraffin block | 1 block |
| Tissue (Snap-frozen) | Tissue cassette | 50 mg |
| Tissue (Snap-frozen) | Cryogenic tube | 50 mg |
| OCT-embedded tissue | Tissue cassette | 50 mg |
| OCT-embedded tissue | Cryogenic tube | 50 mg |
| Tissue (Fresh) | Tissue culture transport media | 50 mg |
| Tissue (Fresh) | Sterile container with saline | 50 mg |
| Bone Marrow | 4 mL Purple tube (EDTA) | 4 mL |
| Tissue scrolls (FFPE) Sterile container | Sterile container | 10-15 scrolls |
| RNA *Must be extracted in a CLIA approved lab | Microcentrifuge tube | Contact IGM lab |
If you are an external healthcare provider with no access to Nationwide Children’s Epic system, submission of a completed Oncology Genetic Test Requisition Form is required. If you are an internal ordering provider with access to Nationwide Children’s Epic system, no requisition form is required; please place the lab order electronically in Epic.
Submission of 10-15 unstained slides at 5-10 microns is recommended. If submitting tissue scroll specimen, please use this Tissue Scroll Calculator tool to determine the thickness and number of tissue scrolls to send to the laboratory.
SPECIMEN INFORMATION:
Fresh and snap-frozen tissue specimens must contain minimum of 10% disease content. Formalin-fixed paraffin embedded (FFPE) tissue block and FFPE tissue scroll specimens must contain minimum of 25% disease content. Please submit the pathology report from the submitted sample. Internal pathology review will also be performed on non-frozen samples to determine the tumor content in specimen.
If sending FFPE tissue scrolls, an H&E slide from a consecutive cut is also required. Please see scroll calculator application above.
All specimens must be labeled with a patient name and one other identifier (DOB, MRN, Specimen ID#, etc). Sample acquisition prior to receiving therapy is strongly preferred.
SPECIMEN SHIPPING:
Specimens must be shipped as soon as possible after collection due to time-sensitive specimen stability. Ship all samples via overnight courier. Whenever possible, send specimen immediately after collection. For fresh tumor tissue, ship the sample refrigerated using cold packs. If specimen cannot be sent on the collection date, then snap-freeze the tumor tissue immediately after collection and ship the sample frozen (bury the entire tissue in dry ice). Lab must receive specimen within 48 hours. FFPE tissue and FFPE tissue scrolls should be sent at room temperature via overnight shipping. Saturday deliveries are accepted; please check Saturday Delivery on shipment labels.
| Overview/Billing |
| Interpretation |
| NCH Lab Only |
| Specimen Type | Type of Container | Volume of Specimen | Status |
|---|---|---|---|
| Unstained tissue slides | Unstained slides and consecutive cut H&E | 10 slides, 10 microns thick | Preferred |
| Paraffin embedded tissue | Paraffin block | 1 block | Preferred |
| Tissue (Snap-frozen) | Tissue cassette | 50 mg | Alternate |
| Tissue (Snap-frozen) | Cryogenic tube | 50 mg | Alternate |
| OCT-embedded tissue | Tissue cassette | 50 mg | Alternate |
| OCT-embedded tissue | Cryogenic tube | 50 mg | Alternate |
| Tissue (Fresh) | Tissue culture transport media | 50 mg | Alternate |
| Tissue (Fresh) | Sterile container with saline | 50 mg | Alternate |
| Bone Marrow | 4 mL Purple tube (EDTA) | 4 mL | Alternate |
| Tissue scrolls (FFPE) Sterile container | Sterile container | 10-15 scrolls | Alternate |
| RNA *Must be extracted in a CLIA approved lab | Microcentrifuge tube | Contact IGM lab | Alternate |
| Specimen Type | Type of Container | Minimum Volume |
|---|---|---|
| Unstained tissue slides | Unstained slides and consecutive cut H&E | 10 slides, 10 microns thick |
| Paraffin embedded tissue | Paraffin block | 1 block |
| Tissue (Snap-frozen) | Tissue cassette | 50 mg |
| Tissue (Snap-frozen) | Cryogenic tube | 50 mg |
| OCT-embedded tissue | Tissue cassette | 50 mg |
| OCT-embedded tissue | Cryogenic tube | 50 mg |
| Tissue (Fresh) | Tissue culture transport media | 50 mg |
| Tissue (Fresh) | Sterile container with saline | 50 mg |
| Bone Marrow | 4 mL Purple tube (EDTA) | 4 mL |
| Tissue scrolls (FFPE) Sterile container | Sterile container | 10-15 scrolls |
| RNA *Must be extracted in a CLIA approved lab | Microcentrifuge tube | Contact IGM lab |
Unstained slides:
Keep at room temperature
Protect from heat
Must be accompanied by H&E slide from adjacent section
Samples decalcified with a strong acid are not accepted. EDTA-based decalcification methods (such as Formical) are acceptable.
Paraffin embedded tissue:
Protect from heat
Keep at room temperature
Tissue (Snap-frozen):
Freeze immediately after collection.
Transport to laboratory as soon as possible
Protect from heat
Keep frozen
OCT-embedded tissue:
Keep frozen
Transport to laboratory as soon as possible
Protect from heat
Freeze immediately after collection.
Tissue (Fresh):
Refrigerate after collection. If delay in transport is greater than 24 hours, freeze.
Transport to lab on ice immediately after collection
Protect from heat
Do not add fixative
Do not formalin fix the sample
Keep refrigerated
Bone marrow:
If delay in transport is greater than 24 hours, refrigerate
Transport to the lab immediately after collection
Room temperature 24 hours
If you are an external healthcare provider with no access to Nationwide Children’s Epic system, submission of a completed Oncology Genetic Test Requisition Form is required. If you are an internal ordering provider with access to Nationwide Children’s Epic system, no requisition form is required; please place the lab order electronically in Epic.
Submission of 10-15 unstained slides at 5-10 microns is recommended. If submitting tissue scroll specimen, please use this Tissue Scroll Calculator tool to determine the thickness and number of tissue scrolls to send to the laboratory.
SPECIMEN INFORMATION:
Fresh and snap-frozen tissue specimens must contain minimum of 10% disease content. Formalin-fixed paraffin embedded (FFPE) tissue block and FFPE tissue scroll specimens must contain minimum of 25% disease content. Please submit the pathology report from the submitted sample. Internal pathology review will also be performed on non-frozen samples to determine the tumor content in specimen.
If sending FFPE tissue scrolls, an H&E slide from a consecutive cut is also required. Please see scroll calculator application above.
All specimens must be labeled with a patient name and one other identifier (DOB, MRN, Specimen ID#, etc). Sample acquisition prior to receiving therapy is strongly preferred.
SPECIMEN SHIPPING:
Specimens must be shipped as soon as possible after collection due to time-sensitive specimen stability. Ship all samples via overnight courier. Whenever possible, send specimen immediately after collection. For fresh tumor tissue, ship the sample refrigerated using cold packs. If specimen cannot be sent on the collection date, then snap-freeze the tumor tissue immediately after collection and ship the sample frozen (bury the entire tissue in dry ice). Lab must receive specimen within 48 hours. FFPE tissue and FFPE tissue scrolls should be sent at room temperature via overnight shipping. Saturday deliveries are accepted; please check Saturday Delivery on shipment labels.
Not New York State approved
