5 USS cut 5-10um thick tumor tissue (at least 5x5mm, preferably 10x10mm)
RECOMMENDATIONS: Send accompanying H&E
Unacceptable Conditions
Paraffin blocks with low tumor percentage
Tissue fixed in heavy metal fixative
Decalcified tissue
Storage/Transport Temperature
Ambient temperature
Performed
Molecular Diagnostics
Notes
TEST DESCRIPTION: The Solid Tumor Precision Panel is for assessment of molecular genetic biomarker targets for FDA-approved therapies in solid tumors. This gene panel will assess 49 genes (see below) for somatically-derived, selected hotspot mutations including single nucleotide variants [SNVs], small insertions or deletions [indels], copy number variations [CNV] and structural variants [inter-genetic and intra-genetic fusions].
TEST DETAILS: Hotspot regions in the following genes are tested for variants with potential clinical utillity.
SNVs and Indels (DNA)-
ATK1
CDKN2A
FGFR1
HRAS
MTOR
RAF1
AKT2
CHECK2
FGFR2
IDH1
NRAS
RET
AKT3
CTNNB1
FGFR3
IDH2
NTRK1
ROS1
ALK
EGFR
FGFR4
KIT
NTRK2
SMO
AR
ERBB2
FLT3
KRAS
NTRK3
TP53
ARAF
ERBB3
GNA11
MAP2K1
PDGFRA
BRAF
ERBB4
GNAQ
MAP2K2
PIK3CA
CDK4
ESR1
GNAS
MET
PTEN
CNVs (DNA)-
CDKN2A del
PTEN del
EGFR amp
ERBB2 amp
FGFR1 amp
MET amp
Fusions (RNA)-
ALK
MET
RSPO2
AR
NRG1
RSPO3
BRAF
NTRK1
EGFR
NTRK2
ESR1
NTRK3
FGFR1
NUTM1
FGFR2
RET
FGFR3
ROS1
Note: This assay is designed to identify well-characterized Tier 1 and Tier 2 hotspot variants [CAP/ASCO/AMP Guidelines; Li, M. et. al. JMD 2017 19(1):4-23] and does not assess full genes. For specific gene panel coverage information, please contact the laboratory.
LIMITATIONS: The following limits of detection have been validated for this assay.
RECOMMENDATIONS: This new gene panel is especially recommended for the subpanel tumor types (Lung, Colorectal, Melanoma, Gastrointestinal stromal tumors-GIST). Other panel testing may be indicated for certian solid tumors (e.g. ovarian, endometrial, breast, prostate), depending on therapeutic considerations (e.g. PARP inhibitors, etc.).
Ordering Recommendations
Replaces the 50 Gene Cancer Panel, Lung Cancer Panel, Colorectal Panel, Melanoma Panel, and the Gastrointestinal stromal tumors-GIST panel)
Performed
Molecular Diagnostics
Synonyms
Lung Cancer (ALK, ROS1, RET)
Colorectal Cancer (BRAF, CHEK2, HRAS, KRAS, NRAS, PIK3CA)
81445
UHC reference code: 56200048
*This test will either require prior authorization or a signed ABN
Test Build Information
OrderCode
OrderName
ResultCode
ResultName
CPT
STPP
Solid Tumor Precision Panel
STPPR
Solid Tumor Precision Panel
81445
Collection
Collect
FIXED (any specimen type):
tissue, paraffin emedded block
tissue, unstained slides (USS)
5 USS cut 5-10um thick tumor tissue (at least 5x5mm, preferably 10x10mm)
RECOMMENDATIONS: Send accompanying H&E
Unacceptable Conditions
Paraffin blocks with low tumor percentage
Tissue fixed in heavy metal fixative
Decalcified tissue
Storage/Transport Temperature
Ambient temperature
Performed
Molecular Diagnostics
Notes
TEST DESCRIPTION: The Solid Tumor Precision Panel is for assessment of molecular genetic biomarker targets for FDA-approved therapies in solid tumors. This gene panel will assess 49 genes (see below) for somatically-derived, selected hotspot mutations including single nucleotide variants [SNVs], small insertions or deletions [indels], copy number variations [CNV] and structural variants [inter-genetic and intra-genetic fusions].
TEST DETAILS: Hotspot regions in the following genes are tested for variants with potential clinical utillity.
SNVs and Indels (DNA)-
ATK1
CDKN2A
FGFR1
HRAS
MTOR
RAF1
AKT2
CHECK2
FGFR2
IDH1
NRAS
RET
AKT3
CTNNB1
FGFR3
IDH2
NTRK1
ROS1
ALK
EGFR
FGFR4
KIT
NTRK2
SMO
AR
ERBB2
FLT3
KRAS
NTRK3
TP53
ARAF
ERBB3
GNA11
MAP2K1
PDGFRA
BRAF
ERBB4
GNAQ
MAP2K2
PIK3CA
CDK4
ESR1
GNAS
MET
PTEN
CNVs (DNA)-
CDKN2A del
PTEN del
EGFR amp
ERBB2 amp
FGFR1 amp
MET amp
Fusions (RNA)-
ALK
MET
RSPO2
AR
NRG1
RSPO3
BRAF
NTRK1
EGFR
NTRK2
ESR1
NTRK3
FGFR1
NUTM1
FGFR2
RET
FGFR3
ROS1
Note: This assay is designed to identify well-characterized Tier 1 and Tier 2 hotspot variants [CAP/ASCO/AMP Guidelines; Li, M. et. al. JMD 2017 19(1):4-23] and does not assess full genes. For specific gene panel coverage information, please contact the laboratory.
LIMITATIONS: The following limits of detection have been validated for this assay.
RECOMMENDATIONS: This new gene panel is especially recommended for the subpanel tumor types (Lung, Colorectal, Melanoma, Gastrointestinal stromal tumors-GIST). Other panel testing may be indicated for certian solid tumors (e.g. ovarian, endometrial, breast, prostate), depending on therapeutic considerations (e.g. PARP inhibitors, etc.).
Ordering
Ordering Recommendations
Replaces the 50 Gene Cancer Panel, Lung Cancer Panel, Colorectal Panel, Melanoma Panel, and the Gastrointestinal stromal tumors-GIST panel)
Performed
Molecular Diagnostics
Synonyms
Lung Cancer (ALK, ROS1, RET)
Colorectal Cancer (BRAF, CHEK2, HRAS, KRAS, NRAS, PIK3CA)