Patient Preparation

No special patient preparations are necessary.

Collect

Tube type:

 One 6.0 mL K2 EDTA (Lavender) tube

   Volume Requirement:

   Optimum: 6.0 mL whole blood

   Minimum: 1.0 mL whole blood

 

Tube must be mixed immediately after filling with blood.

Labeling:

  • Specimen must be labeled with patient name, hospital number, date &  time collected and name of person who collected specimen.

Unacceptable Conditions

Specimen > 72 hours old; Frozen, clotted or grossly hemolyzed samples.

Regional Pathology Services Transport Information

Provide patient's height and weight in order comments.

Storage/Transport Temperature

Storage:

   Room temperature for 4 hours

   Refrigerator temperature for 0-72 hours

Performed

Hematology.
Daily, routine and STAT.
STAT requests are honored until 2100.

Remarks

The Kleihauer-Betke test is done to determine the amount of fetal blood or transplacental hemorrhage (TPH) present in a mother's circulation. TPH can be present after a baby's delivery. This is of main concern in Rh antigen negative mothers. The presence of fetal blood positive for the Rh antigen can immunize an Rh negative mother to the Rh antigen. Knowing the amount of TPH present is used to determine the correct  prophylactic dose of the immunoglobulin (RhoGAM) to be administered to the mother to protect her from Rh immunization. TPH can also be present after some sort of trauma to the mother or fetus. An example of this could be a "traumatic amniocentisis" where the placenta may have been accidentally nicked and fetal blood has leaked into the mother's circulation or amniotic fluid. Trauma can be of concern in both Rh positive and Rh negative mothers.

Notes

Test determines % fetal cells.
Patient’s height and weight should be provided. These parameters enable more accurate calculation of the volume of fetomaternal bleeding as well as the number of vials of Rh immune globulin to administer to an Rh-negative woman. If the weight &/or height are not given, only the volume of the bleed will be reported. This better serves the patient by allowing for individual determination of the amount of Rh immune globulin to administer. Questions concerning dosage calculations may be directed to the pathology resident on-call (pager 888-1317 or 888-1380) or Dr. Scott Koepsell (pager 888-0374).

Performed

Hematology.
Daily, routine and STAT.
STAT requests are honored until 2100.

Methodology

Fetal Hemoglobin Test kit

Reported

Results within 24 hours.

Reference Interval

Percent Fetal Cells = 0.00%

Critical Value

Percent Fetal Cells > 0.00%

CPT Codes

85460

Test Build Information

OrderCode OrderName ResultCode ResultName ResultUnits LOINC CPT
KLEIH KLEIHAUER-BETKE PFC % FETAL CELLS % 48556-5 85460
KLEIH KLEIHAUER-BETKE KLHT MOTHER'S HEIGHT Inches 8302-2  
KLEIH KLEIHAUER-BETKE KLWT MOTHER'S WEIGHT Lbs 29463-7  
KLEIH KLEIHAUER-BETKE BV MOTHER'S BLOOD VOL mL    
KLEIH KLEIHAUER-BETKE VFMH VOL FETOMATERNAL HEMORRHAGE mL 48555-7  
KLEIH KLEIHAUER-BETKE KBINT K/B INTERPRETATION   59466-3  
KLEIH KLEIHAUER-BETKE RHVIAL NUMBER OF RH VIALS vial(s) 1313-6  
KLEIH KLEIHAUER-BETKE KBSL K/B SLIDE      

Additional Information

For additional information or questions, contact RPSInterfaceSupport@unmc.edu
Collection

Patient Preparation

No special patient preparations are necessary.

Collect

Tube type:

 One 6.0 mL K2 EDTA (Lavender) tube

   Volume Requirement:

   Optimum: 6.0 mL whole blood

   Minimum: 1.0 mL whole blood

 

Tube must be mixed immediately after filling with blood.

Labeling:

  • Specimen must be labeled with patient name, hospital number, date &  time collected and name of person who collected specimen.

Unacceptable Conditions

Specimen > 72 hours old; Frozen, clotted or grossly hemolyzed samples.

Regional Pathology Services Transport Information

Provide patient's height and weight in order comments.

Storage/Transport Temperature

Storage:

   Room temperature for 4 hours

   Refrigerator temperature for 0-72 hours

Performed

Hematology.
Daily, routine and STAT.
STAT requests are honored until 2100.

Remarks

The Kleihauer-Betke test is done to determine the amount of fetal blood or transplacental hemorrhage (TPH) present in a mother's circulation. TPH can be present after a baby's delivery. This is of main concern in Rh antigen negative mothers. The presence of fetal blood positive for the Rh antigen can immunize an Rh negative mother to the Rh antigen. Knowing the amount of TPH present is used to determine the correct  prophylactic dose of the immunoglobulin (RhoGAM) to be administered to the mother to protect her from Rh immunization. TPH can also be present after some sort of trauma to the mother or fetus. An example of this could be a "traumatic amniocentisis" where the placenta may have been accidentally nicked and fetal blood has leaked into the mother's circulation or amniotic fluid. Trauma can be of concern in both Rh positive and Rh negative mothers.

Notes

Test determines % fetal cells.
Patient’s height and weight should be provided. These parameters enable more accurate calculation of the volume of fetomaternal bleeding as well as the number of vials of Rh immune globulin to administer to an Rh-negative woman. If the weight &/or height are not given, only the volume of the bleed will be reported. This better serves the patient by allowing for individual determination of the amount of Rh immune globulin to administer. Questions concerning dosage calculations may be directed to the pathology resident on-call (pager 888-1317 or 888-1380) or Dr. Scott Koepsell (pager 888-0374).

Ordering

Performed

Hematology.
Daily, routine and STAT.
STAT requests are honored until 2100.

Methodology

Fetal Hemoglobin Test kit

Reported

Results within 24 hours.

Result Interpretation

Reference Interval

Percent Fetal Cells = 0.00%

Critical Value

Percent Fetal Cells > 0.00%

Administrative

CPT Codes

85460
RPS Interface Information

Test Build Information

OrderCode OrderName ResultCode ResultName ResultUnits LOINC CPT
KLEIH KLEIHAUER-BETKE PFC % FETAL CELLS % 48556-5 85460
KLEIH KLEIHAUER-BETKE KLHT MOTHER'S HEIGHT Inches 8302-2  
KLEIH KLEIHAUER-BETKE KLWT MOTHER'S WEIGHT Lbs 29463-7  
KLEIH KLEIHAUER-BETKE BV MOTHER'S BLOOD VOL mL    
KLEIH KLEIHAUER-BETKE VFMH VOL FETOMATERNAL HEMORRHAGE mL 48555-7  
KLEIH KLEIHAUER-BETKE KBINT K/B INTERPRETATION   59466-3  
KLEIH KLEIHAUER-BETKE RHVIAL NUMBER OF RH VIALS vial(s) 1313-6  
KLEIH KLEIHAUER-BETKE KBSL K/B SLIDE      

Additional Information

For additional information or questions, contact RPSInterfaceSupport@unmc.edu