Early morning Sputum (expectorated or induced). Source of specimen is required.
If for isolation release protocol, two sputum specimens may be tested per patient, but must be collected at least 8 hours apart.
Unacceptable Conditions
Bloody specimens, leaking containers, Non sputum specimen. Swabs. Specimens with food or solid particles.
Storage/Transport Temperature
Submit 5-15 mL sputum specimen in a sterile leak-proof container refrigerated at 2-8 °C. Place each individual specimen in a sealed bag.
Performed
Microbiology. Monday-Friday, results same day if received in department by 0800 that day. STAT testing is not indicated and should not be performed.
Remarks
Source of specimen indicated on requisition is required.
Testing must be accompanied by an Acid Fast Bacilli Culture order (AFBCU).
Notes
The Mycobacterium tuberculosis (MTB) amplified direct detection test is performed using the Cepheid GeneXpert MTB/RIF Assay which detects MTB complex directly from sputum specimens via real-time PCR. This test can be performed on both acid-fast bacilli (AFB) smear-positive and smear-negative specimens. The test is specific for but does not differentiate between member of the M. tuberculosis complex.
Per CDC recommendations, testing is appropriate for patients with symptoms of active pulmonary tuberculosis when results would impact case management or TB control activities.A first time AFB smear-positive specimen from a suspected tuberculosis patient will be reflexed for MTB Amplified Direct Detection.
Only one specimen per patient will be tested unless testing is being performed for the release from isolation, in which case two specimens collected at least 8 hours apart may be tested.
Performed
Microbiology. Monday-Friday, results same day if received in department by 0800 that day. STAT testing is not indicated and should not be performed.
Methodology
Quantitative Real Time PCR
Reference Interval
Negative
A negative result does not exclude M. tuberculosis infection.
CPT Codes
87556
Test Build Information
Below is the standard RPS build for microbiology cultures. EHR_EMR formatting can be different for some vendors. Please contact RPS Interface Support (RPSInterfaceSupport@unmc.edu) for specific information for the vendor specific formatting.
OrderCode
OrderName
ResultCode
ResultName
Result Units
LOINC
CPT
TBAPR
MTB Amplified Direct
SDES
Specimen Source
31208-2
87556
TBAPR
MTB Amplified Direct
SREQ
Additional Info
48767-8
TBAPR
MTB Amplified Direct
TBPRO
Probe Result
14556-5
TBAPR
MTB Amplified Direct
CULT
Culture Result:
41852-5
TBAPR
MTB Amplified Direct
RPT
Report Status
AOE Information (Ask at Order Entry Questions)
Order Code
Order Description
AOE Code
AOE Name
Answer
Answer code
question type
TBAPR
MTB Amplified Direct
SDES
Specimen Source
See Full list in Test Directory under Sources for RPS Clients.
Early morning Sputum (expectorated or induced). Source of specimen is required.
If for isolation release protocol, two sputum specimens may be tested per patient, but must be collected at least 8 hours apart.
Unacceptable Conditions
Bloody specimens, leaking containers, Non sputum specimen. Swabs. Specimens with food or solid particles.
Storage/Transport Temperature
Submit 5-15 mL sputum specimen in a sterile leak-proof container refrigerated at 2-8 °C. Place each individual specimen in a sealed bag.
Performed
Microbiology. Monday-Friday, results same day if received in department by 0800 that day. STAT testing is not indicated and should not be performed.
Remarks
Source of specimen indicated on requisition is required.
Testing must be accompanied by an Acid Fast Bacilli Culture order (AFBCU).
Notes
The Mycobacterium tuberculosis (MTB) amplified direct detection test is performed using the Cepheid GeneXpert MTB/RIF Assay which detects MTB complex directly from sputum specimens via real-time PCR. This test can be performed on both acid-fast bacilli (AFB) smear-positive and smear-negative specimens. The test is specific for but does not differentiate between member of the M. tuberculosis complex.
Per CDC recommendations, testing is appropriate for patients with symptoms of active pulmonary tuberculosis when results would impact case management or TB control activities.A first time AFB smear-positive specimen from a suspected tuberculosis patient will be reflexed for MTB Amplified Direct Detection.
Only one specimen per patient will be tested unless testing is being performed for the release from isolation, in which case two specimens collected at least 8 hours apart may be tested.
Ordering
Performed
Microbiology. Monday-Friday, results same day if received in department by 0800 that day. STAT testing is not indicated and should not be performed.
Methodology
Quantitative Real Time PCR
Result Interpretation
Reference Interval
Negative
A negative result does not exclude M. tuberculosis infection.
Administrative
CPT Codes
87556
RPS Interface Information
Test Build Information
Below is the standard RPS build for microbiology cultures. EHR_EMR formatting can be different for some vendors. Please contact RPS Interface Support (RPSInterfaceSupport@unmc.edu) for specific information for the vendor specific formatting.
OrderCode
OrderName
ResultCode
ResultName
Result Units
LOINC
CPT
TBAPR
MTB Amplified Direct
SDES
Specimen Source
31208-2
87556
TBAPR
MTB Amplified Direct
SREQ
Additional Info
48767-8
TBAPR
MTB Amplified Direct
TBPRO
Probe Result
14556-5
TBAPR
MTB Amplified Direct
CULT
Culture Result:
41852-5
TBAPR
MTB Amplified Direct
RPT
Report Status
AOE Information (Ask at Order Entry Questions)
Order Code
Order Description
AOE Code
AOE Name
Answer
Answer code
question type
TBAPR
MTB Amplified Direct
SDES
Specimen Source
See Full list in Test Directory under Sources for RPS Clients.
