No special patient preparation is necessary
Tube Type:
One 2.7 mL 3.2% sodium citrate (light-blue top) tube
Alternative: 1.8 mL 3.2% sodium citrate tube (available for pediatric patients or difficult draws)
Also acceptable: Two 0.5 mL aliquots, frozen, platelet poor citrated plasma minimum
Aliquot tubes must be polypropylene
Collection Details:
Venipuncture using a 19- to 21-gauge needle is recommended to minimize hemolysis. If multiple specimens are collected, adhere to policy for order of draw
Tubes MUST be full. DO NOT overfill or underfill
Tube MUST be mixed immediately after filling with blood
Gently mix tube by inverting 5 to 6 times. Vigorous mixing or shaking may cause hemolysis, which will affect the result
DO NOT draw blood into a heparinized syringe or from a heparinized line
If unavoidable:
Flush the line with 5 mL saline, AND
Discard at least the first 5 mL of blood or six dead-space volumes of the vascular access device
For patients with hematocrit >55%, the citrate concentration should be adjusted. A special draw tube is available upon request - call Core Lab 402-559-9113.
Transport room temp
Centrifuge and test within 4 hours.
Specimens with Unfractionated Heparin testing must be centrifuged within 1 hour.
If testing cannot be completed within 4 hours, prepare platelet poor plasma and freeze at -20 C.
To prepare platelet poor plasma:
Centrifuge at ~3700 x g for 10 minutes at room temperature, or other current validated setting for producing platelet poor plasma
Using a plastic disposable pipette, carefully transfer the top ¾ of the plasma into a polypropylene tube, avoiding the buffy coat. DO NOT pour off.
If necessary, repeat centrifugation. Transfer the top ¾ of the plasma from the second spin into a polypropylene tube, again avoiding the buffy coat.
The residual platelet count MUST be < 10,000/uL. Each laboratory must validate its centrifugation method to consistently produce platelet poor plasma.
Inspect the plasma for clots or visible hemolysis.
Cap and freeze at -20 C immediately
Specimens collected in an anticoagulant other than 3.2% sodium citrate
Under or overfilled specimens
Clotted specimens
Grossly hemolyzed, lipemic, or icteric specimens
Refrigerated or frozen whole blood specimens
Coagulation
Daily, Routine or STAT
402-559-9113
Coagulation
Daily, Routine or STAT
402-559-9113
Main Lab: Instrumentation Laboratory ACL Top 750
Oakview: Instrumentation Laboratory ACL Top 300
Results same day.
STAT results within 1 hour.
Routine results within 2 hours.
Normal Values:
Main Lab: PTT: 25.0 - 36.5 seconds
Oakview: PTT: 24.2 - 37.3 seconds
Village Pointe: PTT: 24.5 - 37.5 seconds
Critical Values:
PTT: >100 seconds
Reference ranges are subject to change: each report will reflect the current reference range.
Therapeutic Ranges for Coagulation Therapy
| 85730 |
| OrderCode | OrderName | ResultCode | ResultName | Result Units | LOINC | CPT |
| PTTS | PTT | PTTS | PTT | sec | 14979-9 | 85730 |
| Collection |
No special patient preparation is necessary
Tube Type:
One 2.7 mL 3.2% sodium citrate (light-blue top) tube
Alternative: 1.8 mL 3.2% sodium citrate tube (available for pediatric patients or difficult draws)
Also acceptable: Two 0.5 mL aliquots, frozen, platelet poor citrated plasma minimum
Aliquot tubes must be polypropylene
Collection Details:
Venipuncture using a 19- to 21-gauge needle is recommended to minimize hemolysis. If multiple specimens are collected, adhere to policy for order of draw
Tubes MUST be full. DO NOT overfill or underfill
Tube MUST be mixed immediately after filling with blood
Gently mix tube by inverting 5 to 6 times. Vigorous mixing or shaking may cause hemolysis, which will affect the result
DO NOT draw blood into a heparinized syringe or from a heparinized line
If unavoidable:
Flush the line with 5 mL saline, AND
Discard at least the first 5 mL of blood or six dead-space volumes of the vascular access device
For patients with hematocrit >55%, the citrate concentration should be adjusted. A special draw tube is available upon request - call Core Lab 402-559-9113.
Transport room temp
Centrifuge and test within 4 hours.
Specimens with Unfractionated Heparin testing must be centrifuged within 1 hour.
If testing cannot be completed within 4 hours, prepare platelet poor plasma and freeze at -20 C.
To prepare platelet poor plasma:
Centrifuge at ~3700 x g for 10 minutes at room temperature, or other current validated setting for producing platelet poor plasma
Using a plastic disposable pipette, carefully transfer the top ¾ of the plasma into a polypropylene tube, avoiding the buffy coat. DO NOT pour off.
If necessary, repeat centrifugation. Transfer the top ¾ of the plasma from the second spin into a polypropylene tube, again avoiding the buffy coat.
The residual platelet count MUST be < 10,000/uL. Each laboratory must validate its centrifugation method to consistently produce platelet poor plasma.
Inspect the plasma for clots or visible hemolysis.
Cap and freeze at -20 C immediately
Specimens collected in an anticoagulant other than 3.2% sodium citrate
Under or overfilled specimens
Clotted specimens
Grossly hemolyzed, lipemic, or icteric specimens
Refrigerated or frozen whole blood specimens
Coagulation
Daily, Routine or STAT
402-559-9113
| Ordering |
Coagulation
Daily, Routine or STAT
402-559-9113
Main Lab: Instrumentation Laboratory ACL Top 750
Oakview: Instrumentation Laboratory ACL Top 300
Results same day.
STAT results within 1 hour.
Routine results within 2 hours.
| Result Interpretation |
Normal Values:
Main Lab: PTT: 25.0 - 36.5 seconds
Oakview: PTT: 24.2 - 37.3 seconds
Village Pointe: PTT: 24.5 - 37.5 seconds
Critical Values:
PTT: >100 seconds
Reference ranges are subject to change: each report will reflect the current reference range.
Therapeutic Ranges for Coagulation Therapy
| Administrative |
| 85730 |
| RPS Interface Information |
| OrderCode | OrderName | ResultCode | ResultName | Result Units | LOINC | CPT |
| PTTS | PTT | PTTS | PTT | sec | 14979-9 | 85730 |
