Reflexed ANA Autoantibody Panel - Serum from SST or Red preferred, EDTA or Heparinized Plasma acceptable but not preferred.
Volume Requirement: Optimum: 0.5 mL serum or plasma Minimum: 0.3 mL serum or plasma
Unacceptable Conditions
Body fluids. Heat inactivated, hemolyzed, lipemic or contaminated specimens.
Storage/Transport Temperature
Refrigerated
Stability (from collection to initiation)
Ambient: Separate serum from cells ASAP (within 8 hours). Transport and store refrigerated (2-8 º Celsius) for up to 7 days; For longer storage freeze at -20º Celsius. Avoid multiple freeze/thaw cycles.
Performed
Microbiology/Serology
Remarks
ANA IFA positive at 1:40 or greater will have titer and pattern reported.
ANA IFA titers of 1:160 or greater will reflex to the ANAPN, ANA Autoantibody panel.
Notes
The ANA panel includes an antibody screen, a quantitative Anti-dsDNA result and qaulitative results for Anti-Chromatin, Anti-Ribosomal P, Anti-SS-A (52kD and 60kD), Anti-SS-B, Anti-Sm/RNP, Anti-RNP (A and 68), Anti-Scl-70, Anti-Jo-1, and Anti-Centromere B.
Performed
Microbiology/Serology
Methodology
Indirect fluorescence assay (IFA) using HEp-2000 cells.
ANA Panel: Multiplex Flow Immunoassay
Reported
Monday - Friday if received by 7 am.
Reference Interval
Normal individuals are negative at the < 1:40 screening dilution.
Results are reported with a titer, and nuclear staining pattern, if applicable.
ANA Panel:
dsDNA Reference Ranges
Negative: <= 4 IU/mL
Indeterminant: 5-9 IU/mL
Positive: >= 10 IU/mL
ANA Reference Ranges
(Excluding dsDNA)
Negative: <1.0 AI
Positive: >= 1.0 AI
*IU=International units AI = Antibody index
CPT Codes
86255; if positive titer add 86256; if positive add 86235 x 10; dsDNA 86225
Reflexed ANA Autoantibody Panel - Serum from SST or Red preferred, EDTA or Heparinized Plasma acceptable but not preferred.
Volume Requirement: Optimum: 0.5 mL serum or plasma Minimum: 0.3 mL serum or plasma
Unacceptable Conditions
Body fluids. Heat inactivated, hemolyzed, lipemic or contaminated specimens.
Storage/Transport Temperature
Refrigerated
Stability (from collection to initiation)
Ambient: Separate serum from cells ASAP (within 8 hours). Transport and store refrigerated (2-8 º Celsius) for up to 7 days; For longer storage freeze at -20º Celsius. Avoid multiple freeze/thaw cycles.
Performed
Microbiology/Serology
Remarks
ANA IFA positive at 1:40 or greater will have titer and pattern reported.
ANA IFA titers of 1:160 or greater will reflex to the ANAPN, ANA Autoantibody panel.
Notes
The ANA panel includes an antibody screen, a quantitative Anti-dsDNA result and qaulitative results for Anti-Chromatin, Anti-Ribosomal P, Anti-SS-A (52kD and 60kD), Anti-SS-B, Anti-Sm/RNP, Anti-RNP (A and 68), Anti-Scl-70, Anti-Jo-1, and Anti-Centromere B.
Ordering
Performed
Microbiology/Serology
Methodology
Indirect fluorescence assay (IFA) using HEp-2000 cells.
ANA Panel: Multiplex Flow Immunoassay
Reported
Monday - Friday if received by 7 am.
Result Interpretation
Reference Interval
Normal individuals are negative at the < 1:40 screening dilution.
Results are reported with a titer, and nuclear staining pattern, if applicable.
ANA Panel:
dsDNA Reference Ranges
Negative: <= 4 IU/mL
Indeterminant: 5-9 IU/mL
Positive: >= 10 IU/mL
ANA Reference Ranges
(Excluding dsDNA)
Negative: <1.0 AI
Positive: >= 1.0 AI
*IU=International units AI = Antibody index
Administrative
CPT Codes
86255; if positive titer add 86256; if positive add 86235 x 10; dsDNA 86225
