Nebraska Medicine Clinical Laboratory Test Directory

Patient Preparation

Collect between 6-10 a.m.

Collect

Serum separator tube or green (sodium or lithium heparin).

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum or plasma to an ARUP standard transport tube. (Min: 0.8 mL)

Unacceptable Conditions

EDTA plasma.

Regional Pathology Services Transport Information

Regional Pathology Services would prefer clients to collect 6 mL Red clot tube, centrifuge and send poured off serum.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: 24 hours; Refrigerated: 1 week; Frozen: 6 months

Performed

Sun-Sat

Notes

Please refer to individual components for stability of sample for this test.

The concentration of free testosterone is derived from a mathematical expression based on the constant for the binding of testosterone to sex hormone binding globulin.

Ordering Recommendations

Provides a calculated value for free testosterone concentration using total testosterone measured by mass spectrometry. May be used to evaluate hyperandrogenism in children and cisgender females, monitor testosterone-suppressing hormone therapies (eg, antiandrogens or estrogens), evaluate testosterone status in individuals with protein-binding abnormalities, or evaluate hypogonadism in cisgender males. To compare this test to other testosterone tests, refer to the ARUP Testosterone Tests Comparison table.

Performed

Sun-Sat

Methodology

Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry/Electrochemiluminescent Immunoassay/Calculation

Reported

1-5 days

Synonyms

Testosterone Panel Female

ARUP Test Code

0081056

Reference Interval

Components

Reference Interval

Testosterone by Mass Spec

Age	Male (ng/dL)	Female (ng/dL)
Premature (26-28 weeks)	59-125	5-16
Premature (31-35 weeks)	37-198	5-22
Newborn	75-400	20-64
1-5 months	14-363	Less than 20
6-24 months	Less than 37	Less than 9
2-3 years	Less than 15	Less than 20
4-5 years	Less than 19	Less than 30
6-7 years	Less than 13	Less than 7
8-9 years	2-8	1-11
10-11 years	2-165	3-32
12-13 years	3-619	6-50
14-15 years	31-733	6-52
16-17 years	158-826	9-58
18-39 years	300-1080	9-55
40-59 years	300-890	9-55
60 years and older	300-720	5-32
Premenopausal (18 years and older)	Not Applicable	9-55
Postmenopausal	Not Applicable	5-32
Tanner Stage I	2-15	2-17
Tanner Stage II	3-303	5-40
Tanner Stage III	10-851	10-63
Tanner Stage IV-V	162-847	11-62

Testosterone, Free by Mass Spec

Age	Male (pg/mL)	Female (pg/mL)
1-6 years	Less than 0.6	Less than 0.6
7-9 years	0.1-0.9	0.6-1.8
10-11	0.1-6.3	0.1-3.5
12-13	0.5-98.0	0.9-6.8
14-15	3-138.0	1.2-7.5
16-17	38.0-173.0	1.2-9.9
18 years and older	47-244	Not Applicable
18-30	Not Applicable	0.8-7.4
31-40	Not Applicable	1.3-9.2
41-51	Not Applicable	1.1-5.8
Postmenopausal	Not Applicable	0.6-3.8
Tanner Stage I	Less than or equal to 3.7	Less than 2.2
Tanner Stage II	0.3-21	0.4-4.5
Tanner Stage III	1.0-98.0	1.3-7.5
Tanner Stage IV	35.0-169.0	1.1-15.5
Tanner Stage V	41.0-239.0	0.8-9.2

Sex Hormone Binding Globulin

Age	Male (nmol/L)	Female (nmol/L)
1-30 days	13-85	14-60
31-364 days	70-250	60-215
1-3 years	50-180	60-190
4-6 years	45-175	55-170
7-9 years	28-190	35-170
10-12 years	23-160	17-155
13-15 years	13-140	11-120
16-17 years	10-60	19-145
18-49 years	17-56	25-122
50 years and older	19-76	17-125
Tanner Stage I	26-186	30-173
Tanner Stage II	22-169	16-127
Tanner Stage III	13-104	12-98
Tanner Stage IV	11-60	14-151
Tanner Stage V	11-71	23-165

Interpretive Data

Free testosterone concentration is calculated using total testosterone (measured by mass spectrometry) and the binding constant of testosterone and sex hormone-binding globulin (SHBG).

For individuals on testosterone-suppressing hormone therapies (e.g., antiandrogens or estrogens), refer to cisgender female reference intervals. For a complete set of all established reference intervals, refer to ltd.aruplab.com/Tests/Pub/0081056.

CPT Codes

84402; 84403; 84270

LOINC

2986-8
49042-5
13967-5

ARUP Test Code

0081056

Performing Lab

ARUP Laboratories

Test Build Information

OrderCode	OrderName	ResultCode	ResultName	Result Units	LOINC	CPT
TESTOS	TESTOSTERONE F/T W&C	TSTWC	TESTOSTERONE	ng/dL	2986-8	84270, 84403, 84402
TESTOS	TESTOSTERONE F/T W&C	TFREE	TESTOSTERONE, FREE	pg/mL	49042-5
TESTOS	TESTOSTERONE F/T W&C	SHBG	SEX HORM BIND GLOB	nmol/L	13967-5

Additional Information

For additional information or questions, contact RPSInterfaceSupport@unmc.edu

Collection

Patient Preparation

Collect between 6-10 a.m.

Collect

Serum separator tube or green (sodium or lithium heparin).

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum or plasma to an ARUP standard transport tube. (Min: 0.8 mL)

Unacceptable Conditions

EDTA plasma.

Regional Pathology Services Transport Information

Regional Pathology Services would prefer clients to collect 6 mL Red clot tube, centrifuge and send poured off serum.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: 24 hours; Refrigerated: 1 week; Frozen: 6 months

Performed

Sun-Sat

Notes

Ordering

Ordering Recommendations

Performed

Sun-Sat

Methodology

Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry/Electrochemiluminescent Immunoassay/Calculation

Reported

1-5 days

Synonyms

Testosterone Panel Female

ARUP Test Code

0081056

Result Interpretation

Reference Interval

Components

Reference Interval

Testosterone by Mass Spec

Age	Male (ng/dL)	Female (ng/dL)
Premature (26-28 weeks)	59-125	5-16
Premature (31-35 weeks)	37-198	5-22
Newborn	75-400	20-64
1-5 months	14-363	Less than 20
6-24 months	Less than 37	Less than 9
2-3 years	Less than 15	Less than 20
4-5 years	Less than 19	Less than 30
6-7 years	Less than 13	Less than 7
8-9 years	2-8	1-11
10-11 years	2-165	3-32
12-13 years	3-619	6-50
14-15 years	31-733	6-52
16-17 years	158-826	9-58
18-39 years	300-1080	9-55
40-59 years	300-890	9-55
60 years and older	300-720	5-32
Premenopausal (18 years and older)	Not Applicable	9-55
Postmenopausal	Not Applicable	5-32
Tanner Stage I	2-15	2-17
Tanner Stage II	3-303	5-40
Tanner Stage III	10-851	10-63
Tanner Stage IV-V	162-847	11-62

Testosterone, Free by Mass Spec

Age	Male (pg/mL)	Female (pg/mL)
1-6 years	Less than 0.6	Less than 0.6
7-9 years	0.1-0.9	0.6-1.8
10-11	0.1-6.3	0.1-3.5
12-13	0.5-98.0	0.9-6.8
14-15	3-138.0	1.2-7.5
16-17	38.0-173.0	1.2-9.9
18 years and older	47-244	Not Applicable
18-30	Not Applicable	0.8-7.4
31-40	Not Applicable	1.3-9.2
41-51	Not Applicable	1.1-5.8
Postmenopausal	Not Applicable	0.6-3.8
Tanner Stage I	Less than or equal to 3.7	Less than 2.2
Tanner Stage II	0.3-21	0.4-4.5
Tanner Stage III	1.0-98.0	1.3-7.5
Tanner Stage IV	35.0-169.0	1.1-15.5
Tanner Stage V	41.0-239.0	0.8-9.2

Sex Hormone Binding Globulin

Age	Male (nmol/L)	Female (nmol/L)
1-30 days	13-85	14-60
31-364 days	70-250	60-215
1-3 years	50-180	60-190
4-6 years	45-175	55-170
7-9 years	28-190	35-170
10-12 years	23-160	17-155
13-15 years	13-140	11-120
16-17 years	10-60	19-145
18-49 years	17-56	25-122
50 years and older	19-76	17-125
Tanner Stage I	26-186	30-173
Tanner Stage II	22-169	16-127
Tanner Stage III	13-104	12-98
Tanner Stage IV	11-60	14-151
Tanner Stage V	11-71	23-165

Interpretive Data

Administrative

CPT Codes

84402; 84403; 84270

LOINC

2986-8
49042-5
13967-5

ARUP Test Code

0081056

Performing Lab

ARUP Laboratories

RPS Interface Information

Test Build Information

OrderCode	OrderName	ResultCode	ResultName	Result Units	LOINC	CPT
TESTOS	TESTOSTERONE F/T W&C	TSTWC	TESTOSTERONE	ng/dL	2986-8	84270, 84403, 84402
TESTOS	TESTOSTERONE F/T W&C	TFREE	TESTOSTERONE, FREE	pg/mL	49042-5
TESTOS	TESTOSTERONE F/T W&C	SHBG	SEX HORM BIND GLOB	nmol/L	13967-5

Additional Information

For additional information or questions, contact RPSInterfaceSupport@unmc.edu

ARUP Consult®

Disease Topics

Patient Preparation

Collect

Specimen Preparation

Unacceptable Conditions

Regional Pathology Services Transport Information

Storage/Transport Temperature

Stability (from collection to initiation)

Performed

Notes

Ordering Recommendations

Performed

Methodology

Reported

Synonyms

ARUP Test Code

Reference Interval

Interpretive Data

CPT Codes

LOINC

ARUP Test Code

Performing Lab

Test Build Information

Additional Information

Patient Preparation

Collect

Specimen Preparation

Unacceptable Conditions

Regional Pathology Services Transport Information

Storage/Transport Temperature

Stability (from collection to initiation)

Performed

Notes

Ordering Recommendations

Performed

Methodology

Reported

Synonyms

ARUP Test Code

Reference Interval

Interpretive Data

CPT Codes

LOINC

ARUP Test Code

Performing Lab

Test Build Information

Additional Information

ARUP Consult®

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