Collect

Buccal mucosa lesion, cervical or genital lesion, eye, throat or vesicle swab, bronchoalveolar lavage (BAL), tissue or vesicle fluid.

Specimen Preparation

Fluid: Transfer 3 mL specimen to a sterile container. (Min: 0.5 mL) Also acceptable: Transfer to 3 mL viral transport media (ARUP Supply #12884) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.
Swab or Tissue:
Place in 3 mL viral transport media (ARUP Supply #12884) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.

Unacceptable Conditions

Bone marrow, CSF, or whole blood. Calcium alginate, eSwab, dry, or wood swabs.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

Ambient: 4 hours; Refrigerated: 72 hours; Frozen: Unacceptable

Performed

Sun-Sat

Remarks

Specimen source preferred.

Notes

If DFA is negative or inadequate, then a HSV culture will be added. Additional charges apply.

Sensitivity of DFA methodology is dependent upon adequacy of the specimen. If specimen contains fewer than 20 cells, then the DFA result will be reported as "inadequate."

Ordering Recommendations

A rapid diagnostic test with culture backup for negative DFA results. Test may be helpful in identifying acute herpes simplex virus (HSV) infection in active lesions (eg, vesicles, ulcers, inflammation of mucous membranes). Molecular testing is generally preferred (refer to Herpes Simplex Virus by PCR (0060041)).

Performed

Sun-Sat

Methodology

Direct Fluorescent Antibody Stain/Cell Culture

Reported

Within 24 hours

ARUP Test Code

0060280

Reference Interval

Negative

CPT Codes

87274; 87273; if reflexed, add 87252; 87253

ARUP Test Code

0060280

Performing Lab

ARUP Laboratories

Test Build Information

OrderCode OrderName ResultCode ResultName ResultUnits LOINC CPT
HSVFAC HSV DFA REFLEX CULT HSV1FA HSV1, DFA   5846-1 87274, 87273 if reflexed, add 87252, 87253
HSVFAC HSV DFA REFLEX CULT HSV2FA HSV2, DFA   5849-5  
HSVFAC HSV DFA REFLEX CULT HSVSRC HSVFAC Source   31208-2  

REFLEX Order:
OrderCode OrderName ResultCode ResultName ResultUnits LOINC CPT
VHSVC HSV CULTURE VHSVF Final   16291-7 87252, 87253

AOE Information (Ask at Order Entry Questions)

Order Code Order Description AOE Code AOE Name Answer Answer code question type
HSVFAC HSV DFA REFLEX CULT HSVSRC HSVFAC Source     Freetext

Additional Information

For additional information or questions, contact RPSInterfaceSupport@unmc.edu
Collection

Collect

Buccal mucosa lesion, cervical or genital lesion, eye, throat or vesicle swab, bronchoalveolar lavage (BAL), tissue or vesicle fluid.

Specimen Preparation

Fluid: Transfer 3 mL specimen to a sterile container. (Min: 0.5 mL) Also acceptable: Transfer to 3 mL viral transport media (ARUP Supply #12884) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.
Swab or Tissue:
Place in 3 mL viral transport media (ARUP Supply #12884) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.

Unacceptable Conditions

Bone marrow, CSF, or whole blood. Calcium alginate, eSwab, dry, or wood swabs.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

Ambient: 4 hours; Refrigerated: 72 hours; Frozen: Unacceptable

Performed

Sun-Sat

Remarks

Specimen source preferred.

Notes

If DFA is negative or inadequate, then a HSV culture will be added. Additional charges apply.

Sensitivity of DFA methodology is dependent upon adequacy of the specimen. If specimen contains fewer than 20 cells, then the DFA result will be reported as "inadequate."
Ordering

Ordering Recommendations

A rapid diagnostic test with culture backup for negative DFA results. Test may be helpful in identifying acute herpes simplex virus (HSV) infection in active lesions (eg, vesicles, ulcers, inflammation of mucous membranes). Molecular testing is generally preferred (refer to Herpes Simplex Virus by PCR (0060041)).

Performed

Sun-Sat

Methodology

Direct Fluorescent Antibody Stain/Cell Culture

Reported

Within 24 hours

ARUP Test Code

0060280
Result Interpretation

Reference Interval

Negative
Administrative

CPT Codes

87274; 87273; if reflexed, add 87252; 87253

ARUP Test Code

0060280

Performing Lab

ARUP Laboratories
RPS Interface Information

Test Build Information

OrderCode OrderName ResultCode ResultName ResultUnits LOINC CPT
HSVFAC HSV DFA REFLEX CULT HSV1FA HSV1, DFA   5846-1 87274, 87273 if reflexed, add 87252, 87253
HSVFAC HSV DFA REFLEX CULT HSV2FA HSV2, DFA   5849-5  
HSVFAC HSV DFA REFLEX CULT HSVSRC HSVFAC Source   31208-2  

REFLEX Order:
OrderCode OrderName ResultCode ResultName ResultUnits LOINC CPT
VHSVC HSV CULTURE VHSVF Final   16291-7 87252, 87253

AOE Information (Ask at Order Entry Questions)

Order Code Order Description AOE Code AOE Name Answer Answer code question type
HSVFAC HSV DFA REFLEX CULT HSVSRC HSVFAC Source     Freetext

Additional Information

For additional information or questions, contact RPSInterfaceSupport@unmc.edu