Collect

Formalin-Fixed, paraffin-embedded tissue (FFPE) in microtainer: 3-5 10uM slices or >100K cells
Formalin-Fixed, paraffin-embedded tissue (FFPE) slides: 10 unstained slides with slices of 10uM.
Fresh tissue: min 0.1mL, 0.1 cc
Heme samples : one 5 mL blood in EDTA (lav top), OR one 3 mL bone marrow in EDTA (min 0.5mL or >100K nucleated cells). Blood samples in yellow top (ACD) Vacutainers are also acceptable.
Body Fluids: >200K cells
Fine needle aspirate slides: Minimum volume is dependent on the tumor size and cellularity.  Unfixed, ethanol fixed, or H&E stained – Soak slide in xylene to remove coverslip and dry before transporting to the lab.

Storage/Transport Temperature

20-25°C for several hours acceptable
Protect FFPE samples from heat exposure, especially in summer months.
Fresh tissue should be sent in saline soaked gauze, a tube with saline, or tissue culture medium.

Unacceptable Conditions

No tumor tissue. Frozen specimens and specimens fixed/processed in alternative fixatives.

Lab Section

Molecular Diagnostics

Methodology

Targeted Next Generation Sequencing (NGS)

Performed

1x/week

Reported

Expected TAT: 7-14 working days

Performing Laboratory Website (click below)

Notes

35-gene Solid Tumor NGS panel:  AKT1, ALK, AR, BRAF, CDK4, CTNNB1, DDR2, EGFR, ERBB2, ERBB4, ESR1, FGFR2, FGFR3, GNA11, GNAQ, HRAS, IDH1, IDH2, JAK1, JAK2, JAK3, KIT, KRAS, MAP2K1, MAP2K2, MET, MTOR, NRAS, PDGFRA, PIK3CA, RAF1, RET, ROS1, SMO

Interpretive Data

Resulted as part of Pathology report.

CPT Codes

81445

CPT Codes

81445
Specimen Requirements

Collect

Formalin-Fixed, paraffin-embedded tissue (FFPE) in microtainer: 3-5 10uM slices or >100K cells
Formalin-Fixed, paraffin-embedded tissue (FFPE) slides: 10 unstained slides with slices of 10uM.
Fresh tissue: min 0.1mL, 0.1 cc
Heme samples : one 5 mL blood in EDTA (lav top), OR one 3 mL bone marrow in EDTA (min 0.5mL or >100K nucleated cells). Blood samples in yellow top (ACD) Vacutainers are also acceptable.
Body Fluids: >200K cells
Fine needle aspirate slides: Minimum volume is dependent on the tumor size and cellularity.  Unfixed, ethanol fixed, or H&E stained – Soak slide in xylene to remove coverslip and dry before transporting to the lab.

Storage/Transport Temperature

20-25°C for several hours acceptable
Protect FFPE samples from heat exposure, especially in summer months.
Fresh tissue should be sent in saline soaked gauze, a tube with saline, or tissue culture medium.

Unacceptable Conditions

No tumor tissue. Frozen specimens and specimens fixed/processed in alternative fixatives.
Testing

Lab Section

Molecular Diagnostics

Methodology

Targeted Next Generation Sequencing (NGS)

Performed

1x/week

Reported

Expected TAT: 7-14 working days

Performing Laboratory Website (click below)

Notes

35-gene Solid Tumor NGS panel:  AKT1, ALK, AR, BRAF, CDK4, CTNNB1, DDR2, EGFR, ERBB2, ERBB4, ESR1, FGFR2, FGFR3, GNA11, GNAQ, HRAS, IDH1, IDH2, JAK1, JAK2, JAK3, KIT, KRAS, MAP2K1, MAP2K2, MET, MTOR, NRAS, PDGFRA, PIK3CA, RAF1, RET, ROS1, SMO
Result Interpretation

Interpretive Data

Resulted as part of Pathology report.
Coding

CPT Codes

81445
URM Labs Internal
Test Build

CPT Codes

81445