5.0 mL blood in plain red top tube(s). Green (sodium heparin), lavender (EDTA) also acceptable. NO Gel Separator. Separate serum/plasma from cells as soon as possible. Refrigerated as soon as possible after collection and stored frozen (-20°C or colder) if NOT analyzed within 24 hours.
Specimen Required
2.0 mL serum/plasma
Specimen Min Vol
1.0 mL serum/plasma
Specimen Preparation
Spin down and separate serum/plasma as soon as possible.
Storage/Transport Temperature
Refer to Specimen stability criteria as shown below
Stability (from collection to initiation)
After Separation from cells:
Ambient
4 hours
Refrigerated
1 week
Frozen
3 months
Unacceptable Conditions
Specimens that exceed stated stability, unlabeled/mislabeled/mismatched specimens, specimens submitted in leaking containers, tube with gel separator
Special Instructions
Time and date of patient's last dose is on the request form.
CPT Codes
80204
Methodology
Enzyme Immunoassay (EMIT)
Performing Location
Chemistry (Toxicology)
Reported STAT
2 - 4 hours
Reported Routine
1 - 2 days
Additional Information
Test developed and performance characteristics determined by University of California Irvine Medical Center, Department of Pathology and Laboratory Medicine. It has not been cleared or approved by the FDA, however, FDA approval is not required for clinical use.
Test set up frequency
Daily
Reference Interval
<0.1 µmol/L Nontoxic drug concentration after 72 hours
Following 4-6 hours Intravenous Methotrexate High Dose Infusion Therapy with dosages ranging from 50 mg/m2 to to 15 g/m2, a patient is at increased risk of developing toxicity at the following levels:
Toxic Levels:
>10.0 µmol/L 24 hours after high dose therapy
>1.0 µmol/L 48 hours after high dose therapy
>0.2 µmol/L 72 hours after high dose therapy
Additional Information
Test developed and performance characteristics determined by University of California Irvine Medical Center, Department of Pathology and Laboratory Medicine. It has not been cleared or approved by the FDA, however, FDA approval is not required for clinical use.
Collection
Collect
5.0 mL blood in plain red top tube(s). Green (sodium heparin), lavender (EDTA) also acceptable. NO Gel Separator. Separate serum/plasma from cells as soon as possible. Refrigerated as soon as possible after collection and stored frozen (-20°C or colder) if NOT analyzed within 24 hours.
Specimen Required
2.0 mL serum/plasma
Specimen Min Vol
1.0 mL serum/plasma
Specimen Preparation
Spin down and separate serum/plasma as soon as possible.
Storage/Transport Temperature
Refer to Specimen stability criteria as shown below
Stability (from collection to initiation)
After Separation from cells:
Ambient
4 hours
Refrigerated
1 week
Frozen
3 months
Unacceptable Conditions
Specimens that exceed stated stability, unlabeled/mislabeled/mismatched specimens, specimens submitted in leaking containers, tube with gel separator
Special Instructions
Time and date of patient's last dose is on the request form.
Test Info
CPT Codes
80204
Methodology
Enzyme Immunoassay (EMIT)
Performing Location
Chemistry (Toxicology)
Reported STAT
2 - 4 hours
Reported Routine
1 - 2 days
Additional Information
Test developed and performance characteristics determined by University of California Irvine Medical Center, Department of Pathology and Laboratory Medicine. It has not been cleared or approved by the FDA, however, FDA approval is not required for clinical use.
Test set up frequency
Daily
Result Interpretation
Reference Interval
<0.1 µmol/L Nontoxic drug concentration after 72 hours
Following 4-6 hours Intravenous Methotrexate High Dose Infusion Therapy with dosages ranging from 50 mg/m2 to to 15 g/m2, a patient is at increased risk of developing toxicity at the following levels:
Toxic Levels:
>10.0 µmol/L 24 hours after high dose therapy
>1.0 µmol/L 48 hours after high dose therapy
>0.2 µmol/L 72 hours after high dose therapy
Additional Information
Test developed and performance characteristics determined by University of California Irvine Medical Center, Department of Pathology and Laboratory Medicine. It has not been cleared or approved by the FDA, however, FDA approval is not required for clinical use.
