Collect

4.0 mL whole blood from plain red-top tube (no gel separator)

Specimen Required

1.0 mL serum

Specimen Min Vol

0.5 mL serum

Specimen Preparation

Spindown and separate serum

Storage/Transport Temperature

Refer to Specimen stability criteria as shown below

Stability (from collection to initiation)

After Separation from cells:

Ambient

7 days

Refrigerated

2 weeks

Frozen

1 month

Unacceptable Conditions

Specimens that exceed stated stability, unlabeled/mislabeled/mismatched specimens, specimens submitted in leaking containers

Special Instructions

None

CPT Codes

80345

Synonyms

  • Nembutal

Methodology

Gas Chromatography/Mass Spectrometry (GCMS)

Performing Location

Chemistry (Toxicology)

Reported STAT

4 - 6 hours

Reported Routine

8 - 12 hours

Additional Information

None

Test set up frequency

Daily

Reference Interval

Pentobarbital: 1 - 5 mcg/mL

Toxic Concentration (automatic call-back): >5 mcg/mL

Treatment for intracranial pressure with supportive therapy:

Children <16 years: 20 - 30 mcg/mL

Adults: 25 - 35 mcg/mL

Additional Information

None
Collection

Collect

4.0 mL whole blood from plain red-top tube (no gel separator)

Specimen Required

1.0 mL serum

Specimen Min Vol

0.5 mL serum

Specimen Preparation

Spindown and separate serum

Storage/Transport Temperature

Refer to Specimen stability criteria as shown below

Stability (from collection to initiation)

After Separation from cells:

Ambient

7 days

Refrigerated

2 weeks

Frozen

1 month

Unacceptable Conditions

Specimens that exceed stated stability, unlabeled/mislabeled/mismatched specimens, specimens submitted in leaking containers

Special Instructions

None
Test Info

CPT Codes

80345

Synonyms

  • Nembutal

Methodology

Gas Chromatography/Mass Spectrometry (GCMS)

Performing Location

Chemistry (Toxicology)

Reported STAT

4 - 6 hours

Reported Routine

8 - 12 hours

Additional Information

None

Test set up frequency

Daily
Result Interpretation

Reference Interval

Pentobarbital: 1 - 5 mcg/mL

Toxic Concentration (automatic call-back): >5 mcg/mL

Treatment for intracranial pressure with supportive therapy:

Children <16 years: 20 - 30 mcg/mL

Adults: 25 - 35 mcg/mL

Additional Information

None
NICU/Pediatric Info

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