Tests Included

If insufficient sample volume is received for an infant, a dilution protocol will be performed

Performed

Monday - Friday

Methodology

Quantitative Real-Time Polymerase Chain Reaction

Reported

Routine: 1 - 3 days

Synonyms

  • Human Immunodeficiency Virus 1
  • HIV Quantitative PCR
  • HIV-1 Quantitative RNA PCR
  • HIV Quantitative Viral Load
  • HIV-1 Quantitation
  • HIV-1 RNA Viral Load
  • HIV Viral Load
  • HIV Detection, PCR
  • HIV RNA Quant
  • HIV PCR
  • HIV Quant PCR
  • HIV QNT
  • Viral Load Monitoring, HIV-1
  • HIV-1 Viral Load Monitoring
  • HIV Viral Load Monitoring
  • Acquired Immune Deficiency Syndrome (AIDS)
  • Molecular Microbiology

Performing Lab

Microbiology

Add-on Eligibility

Yes
*Add-on orders can be accepted for specimens already submitted to the Molecular Microbiology Lab for testing. Samples processed and stored in other lab departments cannot be used for add-on testing for Molecular Microbiology tests.

Specimen Type

Blood

Specimen Preparation

Specimens must be centrifuged and separated from cells within 24 hours of the collection time for optimal sample integrity.

Specimen Volume

Adult: 6 mL (Minimum: 2 mL)
Pediatric: 1 mL (Minimum: 1 mL)

Collection Container

EDTA Whole Blood Tube (Lavender Top Vacutainer)

Pediatric Collection

2 EDTA Microtainers (Lavender Top Microtainers)
     

Unacceptable Conditions

  1. More than one specimen within 7 days
  2. Lipemic or grossly hemolyzed specimens
  3. Specimens that have been improperly collected, stored, or transported
  • Specimens received in a collection tube other than an EDTA Whole Blood Tube
  • Plasma not separated within 24 hours of collection
  • Whole blood or specimens in primary tubes that have been stored frozen
  1. Specimens containing insufficient volume of plasma once separated from cells
  2. Unlabeled or mislabeled specimens

Storage/Transport Temperature

Transport Instructions  
Collection LocationTransport TemperatureProcessing RequiredTimeframe
ED/InpatientRoom TemperatureNoneSpecimen must be received by the lab within 24 hours of collection
Laboratory/
Outpatient/
Off-Site		Room TemperatureNoneSpecimen must be received by the lab within 24 hours of collection
Refrigerated (Ice Packs)NoneSpecimen must be received by the lab within 24 hours of collection
Refrigerated (Ice Packs)Centrifuge and separate from cells within
24 hours of collection. Send plasma aliquot.		Specimen must be received by the lab within 6 days of collection

Storage: Refrigerated

Stability (from collection to initiation)

Stability:
Prior to separation from cells:
  • Room Temperature: 24 hours
  • Refrigerated: 24 hours
After separation from cells:
  • Refrigerated: 6 days
  • Frozen at -20°C: 12 weeks
  • Frozen at -80°C: 6 months

Laboratory Storage: Frozen at -80°C
Laboratory Retention: 3 months

Remarks

When multiple tests are ordered, a separate specimen must be submitted for each requested test.

Collection Instructions

Labeling Instructions:
  1. When labeling blood tubes, leave a small window visible for the lab to assess the fill volume and sample integrity
  2. Ensure that the barcode is in the correct orientation


Collection Instructions:
  1. Follow the correct order of draw when collecting with additional orders and tube types:
     

Processing Instructions:
  1. Invert the collection tube according to the number of inversions in the chart above
  2. Centrifuge and separate from cells within 24 hours of collection

Reference Interval

Not Detected

Interpretive Data

The quantitative range of this assay is 20 - 10,000,000 copies/mL. An interpretation of "Not Detected" does not rule out the presence of HIV virus RNA concentrations below the level of detection of the assay.

If the assay did not detect the virus, the test result will be reported as "Not Detected". If the assay detected the presence of the virus below the quantitation limit (20 cp/mL) but was not able to accurately quantify the number of copies, the test result will be reported as "<20 cp/mL". This test has been approved by the US Food and Drug Administration (FDA).
 
HIV Quantitative Interpretation
Result Interpretation
Not Detected HIV-1 RNA Not Detected
<20 copies/mL HIV-1 RNA Detected
Calculated titer is below the Lower Limit of Quantitation (LLoQ) of the assay.
Titer
(20 - 10,000,000 copies/mL)		 HIV-1 RNA Detected
Calculated titer is within the Linear Range of the assay – greater than or equal to 20 copies/mL and less than or equal to 10,000,000 copies/mL.
>10,000,000 copies/mL HIV-1 RNA Detected
Calculated titer is above the Upper Limit of Quantitation (ULoQ) of the assay of 10,000,000 copies/mL.
Ordering

Tests Included

If insufficient sample volume is received for an infant, a dilution protocol will be performed

Performed

Monday - Friday

Methodology

Quantitative Real-Time Polymerase Chain Reaction

Reported

Routine: 1 - 3 days

Synonyms

  • Human Immunodeficiency Virus 1
  • HIV Quantitative PCR
  • HIV-1 Quantitative RNA PCR
  • HIV Quantitative Viral Load
  • HIV-1 Quantitation
  • HIV-1 RNA Viral Load
  • HIV Viral Load
  • HIV Detection, PCR
  • HIV RNA Quant
  • HIV PCR
  • HIV Quant PCR
  • HIV QNT
  • Viral Load Monitoring, HIV-1
  • HIV-1 Viral Load Monitoring
  • HIV Viral Load Monitoring
  • Acquired Immune Deficiency Syndrome (AIDS)
  • Molecular Microbiology

Performing Lab

Microbiology

Add-on Eligibility

Yes
*Add-on orders can be accepted for specimens already submitted to the Molecular Microbiology Lab for testing. Samples processed and stored in other lab departments cannot be used for add-on testing for Molecular Microbiology tests.
Collection

Specimen Type

Blood

Specimen Preparation

Specimens must be centrifuged and separated from cells within 24 hours of the collection time for optimal sample integrity.

Specimen Volume

Adult: 6 mL (Minimum: 2 mL)
Pediatric: 1 mL (Minimum: 1 mL)

Collection Container

EDTA Whole Blood Tube (Lavender Top Vacutainer)

Pediatric Collection

2 EDTA Microtainers (Lavender Top Microtainers)
     

Unacceptable Conditions

  1. More than one specimen within 7 days
  2. Lipemic or grossly hemolyzed specimens
  3. Specimens that have been improperly collected, stored, or transported
  • Specimens received in a collection tube other than an EDTA Whole Blood Tube
  • Plasma not separated within 24 hours of collection
  • Whole blood or specimens in primary tubes that have been stored frozen
  1. Specimens containing insufficient volume of plasma once separated from cells
  2. Unlabeled or mislabeled specimens

Storage/Transport Temperature

Transport Instructions  
Collection LocationTransport TemperatureProcessing RequiredTimeframe
ED/InpatientRoom TemperatureNoneSpecimen must be received by the lab within 24 hours of collection
Laboratory/
Outpatient/
Off-Site		Room TemperatureNoneSpecimen must be received by the lab within 24 hours of collection
Refrigerated (Ice Packs)NoneSpecimen must be received by the lab within 24 hours of collection
Refrigerated (Ice Packs)Centrifuge and separate from cells within
24 hours of collection. Send plasma aliquot.		Specimen must be received by the lab within 6 days of collection

Storage: Refrigerated

Stability (from collection to initiation)

Stability:
Prior to separation from cells:
  • Room Temperature: 24 hours
  • Refrigerated: 24 hours
After separation from cells:
  • Refrigerated: 6 days
  • Frozen at -20°C: 12 weeks
  • Frozen at -80°C: 6 months

Laboratory Storage: Frozen at -80°C
Laboratory Retention: 3 months

Remarks

When multiple tests are ordered, a separate specimen must be submitted for each requested test.

Collection Instructions

Labeling Instructions:
  1. When labeling blood tubes, leave a small window visible for the lab to assess the fill volume and sample integrity
  2. Ensure that the barcode is in the correct orientation


Collection Instructions:
  1. Follow the correct order of draw when collecting with additional orders and tube types:
     

Processing Instructions:
  1. Invert the collection tube according to the number of inversions in the chart above
  2. Centrifuge and separate from cells within 24 hours of collection
Result Interpretation

Reference Interval

Not Detected

Interpretive Data

The quantitative range of this assay is 20 - 10,000,000 copies/mL. An interpretation of "Not Detected" does not rule out the presence of HIV virus RNA concentrations below the level of detection of the assay.

If the assay did not detect the virus, the test result will be reported as "Not Detected". If the assay detected the presence of the virus below the quantitation limit (20 cp/mL) but was not able to accurately quantify the number of copies, the test result will be reported as "<20 cp/mL". This test has been approved by the US Food and Drug Administration (FDA).
 
HIV Quantitative Interpretation
Result Interpretation
Not Detected HIV-1 RNA Not Detected
<20 copies/mL HIV-1 RNA Detected
Calculated titer is below the Lower Limit of Quantitation (LLoQ) of the assay.
Titer
(20 - 10,000,000 copies/mL)		 HIV-1 RNA Detected
Calculated titer is within the Linear Range of the assay – greater than or equal to 20 copies/mL and less than or equal to 10,000,000 copies/mL.
>10,000,000 copies/mL HIV-1 RNA Detected
Calculated titer is above the Upper Limit of Quantitation (ULoQ) of the assay of 10,000,000 copies/mL.

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