Performed

Daily

Methodology

Turbidimetry

Reported

Routine: 2 - 5 days

Synonyms

  • Kappa/Lambda Quantitative Free Light Chains with Ratio
  • Kappa Free Light Chains, Quantitation
  • Lambda Free Light Chains, Quantitation
  • Kappa/Lambda Free Light Chain Ratio
  • Immunoglobulin Free Light Chains
  • Kappa/Lambda FLC Ratio
  • Kappa FLC
  • CALM Chemistry

Performing Lab

CALM Chemistry

Add-on Eligibility

Yes, within 7 days of collection
*Samples are stored for a maximum of 7 days

Specimen Type

Blood

Specimen Preparation

Blood specimens collected in Serum Separator Tubes (SSTs) must be centrifuged within 2 hours of the collection time for optimal sample integrity.

NOTE: Specimens collected in SSTs MUST sit for 30 minutes prior to centrifugation to allow for complete clot formation.

Specimen Volume

Adult: 6 mL (Minimum: 2 mL)

Pediatric: 0.5 mL (Minimum: 0.5 mL)
  • Microtainer must be FULL

*This refers to specimen volume at collection prior to any processing

Collection Container

Serum Separator Tube (SST, Gold Top Vacutainer)

Pediatric Collection

1 Serum Separator Microtainer (SST, Gold Top Microtainer)
  • Panel tests (BMP/CMP/CBC) require a full microtainer. If additional tests are ordered, an additional microtainer is needed.  Full microtainers should be sufficient for up to 5 individual tests.

Unacceptable Conditions

  1. Grossly hemolyzed or lipemic specimens
  2. Specimens containing particulate matter or exhibiting obvious microbial contamination
  3. Specimens that have been improperly collected, stored, or transported
  • Specimens received in a collection tube other than a Serum or Serum Separator Tube
  1. Specimens containing insufficient volume of serum once separated from cells
  2. Unlabeled or mislabeled specimens

Storage/Transport Temperature

Transport Instructions
Collection Location Transport Temperature Processing Required Timeframe
ED/Inpatient Room Temperature None Specimen must be received by the lab within 2 hours of collection
Refrigerated (Ice Packs) Allow blood to clot for 30 minutes in a vertical position. Centrifuge to separate from cells within 2 hours of collection. Specimen must be received by the lab within 21 days of collection
Laboratory/
Outpatient/
Off-Site		 Room Temperature Allow blood to clot for 30 minutes in a vertical position. Centrifuge to separate from cells within 2 hours of collection. Specimen must be received by the lab within 2 hours of collection
Refrigerated (Ice Packs) Allow blood to clot for 30 minutes in a vertical position. Centrifuge to separate from cells within 2 hours of collection. Specimen must be received by the lab within 21 days of collection
Frozen (Dry Ice) Allow blood to clot for 30 minutes in a vertical position. Centrifuge to separate from cells within 2 hours of collection. Specimen must be received by the lab within 1 year of collection

Storage: Refrigerated

Stability (from collection to initiation)

Stability:
Prior to separation from cells:
  • Room Temperature: 2 hours
After separation from cells:
  • Room Temperature: 2 hours
  • Refrigerated: 21 days
  • Frozen: 1 year
    • Avoid repeated freeze-thaws

Laboratory Storage: Refrigerated
Laboratory Retention: 7 days

Collection Instructions

Labeling Instructions:
  1. When labeling blood tubes, leave a small window visible for the lab to assess the fill volume and sample integrity
  2. Ensure that the barcode is in the correct orientation

Collection Instructions:
  1. Follow the correct order of draw when collecting with additional orders and tube types:
     
 
Processing Instructions:
  1. Invert the collection tube according to the number of inversions in the chart above
  2. Place the tube upright in a rack and allow the tube to sit for 30 minutes to ensure adequate clotting time
  3. Centrifuge within 2 hours of collection

Reference Interval

Kappa/Lambda Free Light Chain Reference Ranges
Test Component Range
Kappa Free Light Chain 3.3 - 19.4 mg/L
Lambda Free Light Chain 5.7 - 26.3 mg/L
Kappa:Lambda Free Light Chain Ratio 0.26 - 1.65
Ordering

Performed

Daily

Methodology

Turbidimetry

Reported

Routine: 2 - 5 days

Synonyms

  • Kappa/Lambda Quantitative Free Light Chains with Ratio
  • Kappa Free Light Chains, Quantitation
  • Lambda Free Light Chains, Quantitation
  • Kappa/Lambda Free Light Chain Ratio
  • Immunoglobulin Free Light Chains
  • Kappa/Lambda FLC Ratio
  • Kappa FLC
  • CALM Chemistry

Performing Lab

CALM Chemistry

Add-on Eligibility

Yes, within 7 days of collection
*Samples are stored for a maximum of 7 days
Collection

Specimen Type

Blood

Specimen Preparation

Blood specimens collected in Serum Separator Tubes (SSTs) must be centrifuged within 2 hours of the collection time for optimal sample integrity.

NOTE: Specimens collected in SSTs MUST sit for 30 minutes prior to centrifugation to allow for complete clot formation.

Specimen Volume

Adult: 6 mL (Minimum: 2 mL)

Pediatric: 0.5 mL (Minimum: 0.5 mL)
  • Microtainer must be FULL

*This refers to specimen volume at collection prior to any processing

Collection Container

Serum Separator Tube (SST, Gold Top Vacutainer)

Pediatric Collection

1 Serum Separator Microtainer (SST, Gold Top Microtainer)
  • Panel tests (BMP/CMP/CBC) require a full microtainer. If additional tests are ordered, an additional microtainer is needed.  Full microtainers should be sufficient for up to 5 individual tests.

Unacceptable Conditions

  1. Grossly hemolyzed or lipemic specimens
  2. Specimens containing particulate matter or exhibiting obvious microbial contamination
  3. Specimens that have been improperly collected, stored, or transported
  • Specimens received in a collection tube other than a Serum or Serum Separator Tube
  1. Specimens containing insufficient volume of serum once separated from cells
  2. Unlabeled or mislabeled specimens

Storage/Transport Temperature

Transport Instructions
Collection Location Transport Temperature Processing Required Timeframe
ED/Inpatient Room Temperature None Specimen must be received by the lab within 2 hours of collection
Refrigerated (Ice Packs) Allow blood to clot for 30 minutes in a vertical position. Centrifuge to separate from cells within 2 hours of collection. Specimen must be received by the lab within 21 days of collection
Laboratory/
Outpatient/
Off-Site		 Room Temperature Allow blood to clot for 30 minutes in a vertical position. Centrifuge to separate from cells within 2 hours of collection. Specimen must be received by the lab within 2 hours of collection
Refrigerated (Ice Packs) Allow blood to clot for 30 minutes in a vertical position. Centrifuge to separate from cells within 2 hours of collection. Specimen must be received by the lab within 21 days of collection
Frozen (Dry Ice) Allow blood to clot for 30 minutes in a vertical position. Centrifuge to separate from cells within 2 hours of collection. Specimen must be received by the lab within 1 year of collection

Storage: Refrigerated

Stability (from collection to initiation)

Stability:
Prior to separation from cells:
  • Room Temperature: 2 hours
After separation from cells:
  • Room Temperature: 2 hours
  • Refrigerated: 21 days
  • Frozen: 1 year
    • Avoid repeated freeze-thaws

Laboratory Storage: Refrigerated
Laboratory Retention: 7 days

Collection Instructions

Labeling Instructions:
  1. When labeling blood tubes, leave a small window visible for the lab to assess the fill volume and sample integrity
  2. Ensure that the barcode is in the correct orientation

Collection Instructions:
  1. Follow the correct order of draw when collecting with additional orders and tube types:
     
 
Processing Instructions:
  1. Invert the collection tube according to the number of inversions in the chart above
  2. Place the tube upright in a rack and allow the tube to sit for 30 minutes to ensure adequate clotting time
  3. Centrifuge within 2 hours of collection
Result Interpretation

Reference Interval

Kappa/Lambda Free Light Chain Reference Ranges
Test Component Range
Kappa Free Light Chain 3.3 - 19.4 mg/L
Lambda Free Light Chain 5.7 - 26.3 mg/L
Kappa:Lambda Free Light Chain Ratio 0.26 - 1.65

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