Performed

Daily

Methodology

Automated Cell Count with Flow Cell Differential

Reported

Routine: 4 hours
Stat: 1 hour

Synonyms

  • Automated Differential
  • Automated CBC
  • NRBC
  • CBC, Automated Differential, NRBC
  • CBC With Differential
  • CBC w/ Differential
  • Blood Cell Count
  • Cell Count
  • Hemogram
  • RBC Count
  • WBC Count
  • HH
  • Hematocrit
  • Hemoglobin
  • Platelet Count
  • Hematology Lab

Performing Lab

Hematology

Add-on Eligibility

Yes, within 48 hours of collection if stored refrigerated

Specimen Type

Blood

Specimen Volume

Adult: 1 mL (Minimum: 0.3 mL - Only if collected in microtainer)

Pediatric: 0.3 mL (Minimum: 0.3 mL)

*This refers to specimen volume at collection prior to any processing

Collection Container

3.0 mL EDTA Whole Blood Tube (Lavender Top Vacutainer)

Pediatric Collection

1 EDTA Microtainer (Lavender Top Microtainer)


OR

1 EDTA Microtainer MAP Microtube (Lavender Top MAP Microtube)

Unacceptable Conditions

1. Frozen specimens
2. Clotted or grossly hemolyzed specimens
3. Unlabeled or mislabeled specimens

Storage/Transport Temperature

Transport Instructions      
Collection Location Transport Temperature Processing Required Timeframe
ED/Inpatient Room Temperature None Specimen must be received by the lab within 24 hours of collection
Laboratory/Outpatient/Off-Site Room Temperature None Specimen must be received by the lab within 24 hours of collection
Refrigerated (Ice Packs) None Specimen must be received by the lab within 48 hours of collection

Storage: Refrigerated

Stability (from collection to initiation)

Stability:
  • Room Temperature: 24 hours
  • Refrigerated: 48 hours
  • Frozen: Unacceptable

Laboratory Storage: Refrigerated
Laboratory Retention: 7 days

Collection Instructions

Labelling Instructions:
  1. When labeling blood tubes, leave a small window visible for the lab to assess the fill volume and sample integrity
  2. Ensure that the barcode is in the correct orientation

Collection Instructions:
  1. Follow the correct order of draw when collecting with additional orders and tube types:
      

Reference Interval

Complete Blood Count with Differentials Reference Ranges    
Test Component 0 Days –
7 Days		 8 Days –
6 Months		 7 Months –
6 Years		 7 Years –
12 Years		 > 12 Years - Adult
WBC (1000/mm3) 8 – 15 6 – 16 6 – 14 4 – 11 4 – 10
RBC (million/mm3) 4.1 – 5.7 2.9 – 4.8 3.8 – 5.0 3.9 – 5.0 Male Female Gender Neutral
4.6 – 6.1 3.9 – 5.2 4.6 - 5.2
Hemoglobin (gm/dL) 13.4 – 20.0 8.9 – 15.3 10.1 – 12.7 10.6 – 13.4 Male Female Gender Neutral
13.7 – 17.5 11.2 – 15.7 13.7 - 15.7
Hematocrit (%) 39 – 57 27 – 45 31 – 38 32 – 40 Male Female Gender Neutral
40 – 50 34 – 45 40 - 45
Reticulocytes (%) 1.1 – 5.4 1.1 – 3.5 0.8 – 1.8 1.0 – 1.9 Male Female Gender Neutral
0.5 – 1.7 0.5 – 1.8 0.5 - 1.7
Absolute Reticulocytes (1000/mm3) 147 – 216 48 – 110 36 – 110 42 – 70 Male Female Gender Neutral
26 – 95 16 – 78 26 - 78
MCV (um3) 91 – 106 74 – 98 70 – 85 74 – 87 79 – 95
MCH (pgm) 31 – 36 24 – 35 23 – 29 25 – 29 26 – 32
MCHC (g/dL) 32 – 36 32 – 36 32 – 36 32 – 36 32 – 36
RDW-CV (%) 14 – 17 12 – 17 12 – 16 12 – 14 12 – 14
MPV (fL) 8.7 – 12.4 8.7 – 12.4 8.7 – 12.4 8.7 – 12.4 9.4 – 12.4
Platelet Count (1000/mm3) 220 – 450 230 – 600 190 – 460 200 – 370 140 – 370
% Bands 0 – 15 0 – 15 0 – 15 0 – 15 0 – 15
Absolute Neutrophil Count (1000/mm3) 3.2 – 11.4 2.5 – 9.4 2.3 – 6.4 2.6 – 6.3  1.6 – 7.0
Absolute Lymphocytes (1000/mm3) 1.8 – 8.0 2.1 – 9.1 1.1 – 8.1 1.0 – 4.3 0.8 – 3.1
Absolute Monocytes (1000/mm3) 0.5 – 1.7 0.3 – 1.4 0.2 – 1.1 0.2 – 0.8 0.2 – 0.8
Absolute Eosinophils (1000/mm3) 0.0 – 0.7 0.0 – 0.8 0.0 – 0.8 0.0 – 0.5 0.0 – 0.5
Absolute Basophils (1000/mm3) <0.2 <0.2 <0.2 <0.2 <0.2
% Immature Granulocytes (IG) ≤2 ≤1 <1 <1 <1
Absolute Immature Granulocytes (1000/mm3) <0.1 – 0.3 <0.1 – 0.2 <0.1 <0.1 <0.1
% Nucleated RBC/100WBC <1 – 8 <1 <1 <1 <1
Absolute Nucleated RBC (1000/mm3) <0.1 – 1.3 <0.1 – 0.1 <0.1 <0.1 <0.1
Ordering

Performed

Daily

Methodology

Automated Cell Count with Flow Cell Differential

Reported

Routine: 4 hours
Stat: 1 hour

Synonyms

  • Automated Differential
  • Automated CBC
  • NRBC
  • CBC, Automated Differential, NRBC
  • CBC With Differential
  • CBC w/ Differential
  • Blood Cell Count
  • Cell Count
  • Hemogram
  • RBC Count
  • WBC Count
  • HH
  • Hematocrit
  • Hemoglobin
  • Platelet Count
  • Hematology Lab

Performing Lab

Hematology

Add-on Eligibility

Yes, within 48 hours of collection if stored refrigerated
Collection

Specimen Type

Blood

Specimen Volume

Adult: 1 mL (Minimum: 0.3 mL - Only if collected in microtainer)

Pediatric: 0.3 mL (Minimum: 0.3 mL)

*This refers to specimen volume at collection prior to any processing

Collection Container

3.0 mL EDTA Whole Blood Tube (Lavender Top Vacutainer)

Pediatric Collection

1 EDTA Microtainer (Lavender Top Microtainer)


OR

1 EDTA Microtainer MAP Microtube (Lavender Top MAP Microtube)

Unacceptable Conditions

1. Frozen specimens
2. Clotted or grossly hemolyzed specimens
3. Unlabeled or mislabeled specimens

Storage/Transport Temperature

Transport Instructions      
Collection Location Transport Temperature Processing Required Timeframe
ED/Inpatient Room Temperature None Specimen must be received by the lab within 24 hours of collection
Laboratory/Outpatient/Off-Site Room Temperature None Specimen must be received by the lab within 24 hours of collection
Refrigerated (Ice Packs) None Specimen must be received by the lab within 48 hours of collection

Storage: Refrigerated

Stability (from collection to initiation)

Stability:
  • Room Temperature: 24 hours
  • Refrigerated: 48 hours
  • Frozen: Unacceptable

Laboratory Storage: Refrigerated
Laboratory Retention: 7 days

Collection Instructions

Labelling Instructions:
  1. When labeling blood tubes, leave a small window visible for the lab to assess the fill volume and sample integrity
  2. Ensure that the barcode is in the correct orientation

Collection Instructions:
  1. Follow the correct order of draw when collecting with additional orders and tube types:
      
Result Interpretation

Reference Interval

Complete Blood Count with Differentials Reference Ranges    
Test Component 0 Days –
7 Days		 8 Days –
6 Months		 7 Months –
6 Years		 7 Years –
12 Years		 > 12 Years - Adult
WBC (1000/mm3) 8 – 15 6 – 16 6 – 14 4 – 11 4 – 10
RBC (million/mm3) 4.1 – 5.7 2.9 – 4.8 3.8 – 5.0 3.9 – 5.0 Male Female Gender Neutral
4.6 – 6.1 3.9 – 5.2 4.6 - 5.2
Hemoglobin (gm/dL) 13.4 – 20.0 8.9 – 15.3 10.1 – 12.7 10.6 – 13.4 Male Female Gender Neutral
13.7 – 17.5 11.2 – 15.7 13.7 - 15.7
Hematocrit (%) 39 – 57 27 – 45 31 – 38 32 – 40 Male Female Gender Neutral
40 – 50 34 – 45 40 - 45
Reticulocytes (%) 1.1 – 5.4 1.1 – 3.5 0.8 – 1.8 1.0 – 1.9 Male Female Gender Neutral
0.5 – 1.7 0.5 – 1.8 0.5 - 1.7
Absolute Reticulocytes (1000/mm3) 147 – 216 48 – 110 36 – 110 42 – 70 Male Female Gender Neutral
26 – 95 16 – 78 26 - 78
MCV (um3) 91 – 106 74 – 98 70 – 85 74 – 87 79 – 95
MCH (pgm) 31 – 36 24 – 35 23 – 29 25 – 29 26 – 32
MCHC (g/dL) 32 – 36 32 – 36 32 – 36 32 – 36 32 – 36
RDW-CV (%) 14 – 17 12 – 17 12 – 16 12 – 14 12 – 14
MPV (fL) 8.7 – 12.4 8.7 – 12.4 8.7 – 12.4 8.7 – 12.4 9.4 – 12.4
Platelet Count (1000/mm3) 220 – 450 230 – 600 190 – 460 200 – 370 140 – 370
% Bands 0 – 15 0 – 15 0 – 15 0 – 15 0 – 15
Absolute Neutrophil Count (1000/mm3) 3.2 – 11.4 2.5 – 9.4 2.3 – 6.4 2.6 – 6.3  1.6 – 7.0
Absolute Lymphocytes (1000/mm3) 1.8 – 8.0 2.1 – 9.1 1.1 – 8.1 1.0 – 4.3 0.8 – 3.1
Absolute Monocytes (1000/mm3) 0.5 – 1.7 0.3 – 1.4 0.2 – 1.1 0.2 – 0.8 0.2 – 0.8
Absolute Eosinophils (1000/mm3) 0.0 – 0.7 0.0 – 0.8 0.0 – 0.8 0.0 – 0.5 0.0 – 0.5
Absolute Basophils (1000/mm3) <0.2 <0.2 <0.2 <0.2 <0.2
% Immature Granulocytes (IG) ≤2 ≤1 <1 <1 <1
Absolute Immature Granulocytes (1000/mm3) <0.1 – 0.3 <0.1 – 0.2 <0.1 <0.1 <0.1
% Nucleated RBC/100WBC <1 – 8 <1 <1 <1 <1
Absolute Nucleated RBC (1000/mm3) <0.1 – 1.3 <0.1 – 0.1 <0.1 <0.1 <0.1

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